• The Korean Journal of Food And Nutrition
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• v.17 no.1
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• pp.47-52
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• 2004

In this study, I attempted to develope the expression and purification system of human mammaglobin proteins in Escherichia coli and to produce anti-human mammaglobin rabbit antibody for the detection of human mammaglobin protein in the peripheral blood of breast cancer patients. Human mammaglobin gene was cloned and sequenced from m-RNAs purified from donated breast cancer tissues using RT-PCR. The cloned gene was inserted into pET30, pET22, and pET32 plasmid. The cloned gene in pET30 yields insoluble proteins which was difficult to purify from the cells extracts. The mammaglobin gene in pET32 was strongly expressed soluble proteins which were isolated using Ni-NTA affinity chromagraphy and DEAE-ion exchange chromatography, followed by enterokinase digestion of the purified proteins. The isolated proteins had enough purity to use as a antigen for the production of anti-mammaglobin antibody in rabbits. The polyclonal antibody produced against the isolated mammaglobin showed a specificity to mammaglobin after Westernblot immuno assay. In conclusion, the isolated mammaglobin protein and the anti-mammaglobin rabbit antibody may be used for diagnosis of breast cancer as well as development of anti-breast cancer drug.

• Clinical and Experimental Reproductive Medicine
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• v.25 no.2
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• pp.115-122
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• 1998

The maintenance of a viable pregnancy has long been viewed as an immunological paradox. The deveolping embryo and trophoblast are immunologically foreign to the maternal immune system due to their maternally inherited genes products and tissue-specific differentiation antigens (Hill & Anderson, 1988). Therefore, speculation has arisen that spontaneous abortion may be caused by impaired maternal immune tolerance to the semiallogenic conceptus (Hill, 1990). Loss of recall antigen has been reported in immunosuppressed transplant recipients and is associated with graft survival (Muluk et al., 1991; Schulik et al., 1994). Progesterone $(10^{-5}M)$ has immunosuppressive capabilities (Szekeres-Bartho et al., 1985). Previous study showed that fertile women, but not women with unexplained recurrent abortion (URA), lose their immune response to recall antigens when pregnant (Bermas & Hill, 1997). Therefore, we hypothesized that immunosuppressive doses of progesterone may affect proliferative response of lymphocytes to trophoblast antigen and alloantigen. Proliferative responses using $^3H$-thymidine ($^3H$-TdR) incorporation of peripheral blood mononuclear cells (PBMCs) to the irradiated allogeneic periperal blood mononuclear cells as alloantigen, trophoblast extract and Flu as recall antigen, and PHA as mitogen were serially checked in 9 women who had experienced unexplained recurrent miscarriage. Progesterone vaginal suppositories (100mg b.i.d; Utrogestan, Organon) beginning 3 days after ovulation were given to 9 women with unexplained RSA who had prior evidence of Th1 immunity to trophoblast. We checked proliferation responses to conception cycle before and after progesterone supplementation once a week through the first 7 weeks of pregnancy. All patients of alloantigen and PHA had a positive proliferation response that occmed in the baseline phase. But 4 out of 9 patients (44.4%) of trophoblast antigen and Flu antigen had a positive proliferative response. The suppression of proliferation response to each antigen were started after proliferative phase and during pregnancy cycles. Our data demonstrated that since in vivo progesterone treated PBMCs suppressed more T-lymphocyte activation and $^3H$-TdR incorporation compare to PBMCs, which are not influenced by progesterone. This data suggested that it might be influenced by immunosuppressive effect of progesterone. In conclusion, progesterone may play an important immunological role in regulating local immune response in the fetal-placental unit. Furthermore, in the 9 women given progesterone during a conception cycle, Only two (22%) repeat pregnancy losses occured in these 9 women despite loss of antigen responsiveness (one chemical pregnancy loss and one loss at 8 weeks of growth which was karyotyped as a Trisomy 4). These finding suggested that pregnancy loss due to fetal aneuploidy is not associated with immunological phenomena.

• Journal of the Society of Naval Architects of Korea
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• v.36 no.2
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• pp.9-21
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• 1999

The waterjet is very widely used today in propulsion system of high speed vessel but manufacturer is limited because of an efficiency and a difficulty of a manufacture on the inlet configuration of the waterjet. The importancy in designing the inlet configuration of the waterjet lies on the minimization of the cavitation which is largely affecting the efficiency. In this paper, the configuration analysis is carried out to find a optimum shape which is minimizing the cavitation using a two dimensional potential-based panel method with an inlet configuration of a flush type. Also, it is developed a direct design method finding an inlet configuration by a given pressure distributions. The numerically obtained optimum shape using this configuration analysis method show a good agreement compared to the Kashiwadani's results. It is carried out a direct design method over a lip and a ramp of an inlet configuration wish pressure distributions obtained a result of the configuration analysis and the results show a good agreement compared to original configuration.

• The Journal of Korean Medicine
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• v.17 no.1 s.31
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• pp.171-189
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• 1996

Background and Purpose: The greater part of patients to visit Hospital of Oriental Medicine suffer from cerebrovascular accident(CVA). There is transcranial Doppler(TCD) in the diagnostic method to confirm cerebrovascular accident(CVA). Transcranial Doppler(TCD) is an accurate method of monitoring the blood flow velocities of the cerebral vessels and have been generally used to prevent symptomatic vasospasm and confirm cerebral infarction. So we studied, in the crebrovascular accident(CVA), to estimate whether transcranial Doppler(TCD) is useful to. Methods: Using transcranial doppler(Multigon Model 500M Transcranial Doppler System), we measured the mean and peak velocity and the direction of blood flow in 10 cerebrovascular accident(CVA)'s subjects who had been examined by Computed Tomography(CT) or Magnetic Resonance Imaging (MRI). Results : As an anticipation, in cerebrovascular accident(CVA)'s subject with Cb-infarction, the mean and peak velocity of blood flow fell down remarkably and the direction of blood flow was change or irritable. But didn't find out any signal in lacunar infarction. Also, in case with spontaneous hemorrhage, the velocity and direction of blood flow was change but this signal was short of diagnosis for Cb-hemorrhage. Besides, we found signals about embolism, stenosis, thrombosis and occlusion in cerebrovascular accident(CVA)'s subjects. Conclusion: In Cb-infarction, the result of TCD was equal to diagnosis with Computed Tomography(CT) or Magnetic Resonance Imaging (MRI). But about lacunar infarction or spontaneous hemorrhage, signals of TCD couldn't be found out or was insufficient more than Computed Tomography(CT) or Magnetic Resonance Imaging(MRI). In cerebrovascular accident(CVA)'s subject with embolism, stenosis, thrombosis or occlusion, signals of TCD were found out more than Computed Tomography(CT) or Magnetic Resonance Imaging(MRI). Therefore transcranial doppler(TCD) is necessary method which makes a diagnosis of cerebrovascular accident(CVA), with Computed Tomography(CT) or Magnetic Resonance Imaging(MRI).

• Journal of Korean Society of Environmental Engineers
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• v.31 no.10
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• pp.927-932
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• 2009

Toxic and recalcitrant organic pollutants in wastewaters can be effectively treated when advanced oxidation and biodegradation are combined, ideally with intimate coupling, in which both processes occur simultaneously in the same system. One means to achieve intimate coupling is to coat nanoscale $TiO_2$ on the outside of macroporous biofilm carriers. This study investigated the kinetics of photocatalysis with $TiO_2$-coated porous carriers. The carriers were made of polyvinyl alcohol (PVA) and coated with $TiO_2$ using a low-temperature sol-gel process. The $TiO_2$-coated carriers catalyzed the oxidation of methylene blue (MB) effectively under irradiation of UV light. The overall reaction rate with adsorption and photolysis saturated at high MB concentration, and approached the adsorption rate, which was first order for all MB concent rations. This result indicates that adsorbed MB may have slowed photocatalysis by blocking active sites for photocatalysis. The overall kinetics could be described by a quasi-Langmuir model. The estimated maximum specific (per unit mass of $TiO_2$) transformation rate of MB by the $TiO_2$-coated carriers was four times larger than that obtained from slurry-$TiO_2$ reactors. This observation demonstrated that the $TiO_2$ present as a coating on the carriers maintained high efficiency for transforming recalcitrant organic matter via photocatalysis. These findings serve as a foundation for advancement of an intimate coupling of photocatalysis to biodegradation.

• Korean Journal of Food Science and Technology
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• v.29 no.3
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• pp.395-399
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• 1997

Meat model emulsions ware prepared with salt-soluble protein and soybean oil. Effects of water-soluble protein (WSP) on the meat model emulsion treated with/without BHT during 8 day storage $5^{\circ}C$ under both dark and light illumination were studied by measuring POV and TBA. An emulsion without BHA and WSP was used as a control. Under light storage, there was no significant difference in peroxide values between the control and the sample treated with BHA except the 2nd day of storage. However, TBA values of the sample treated with BHA were significantly (p<0.05) lower than those of control except the 4th day of storage. TBA and POV of the samples treated with WSP and WSP + BHA were higher than control after 4th day of storage under light. That is, water soluble protein, which was composed mainly of myoglobin, increased lipid oxidation under light storage. The similar trends were also shown in the samples stored under dark. These results suggested that acceleration of lipid oxidation of the meat model emulsions by water soluble protein (WSP) under both light and dark might not be due to the singlet oxygen formation, but due to superoxide anion formed.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 1996.10a
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• pp.239-242
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• 1996

본 논문에서는 BP(Back Propagation)에 비해서 빠른 학습시간과 다른 경쟁학습 신경회로망 알고리즘에 비해서 비교적 우수한 성능으로 패턴인식 등에 많이 이용되고 있는 LVQ(Learning Vector Quantization) 알고리즘의 성능을 향상시키기 위한 방법을 논의하고자 한다. 일반적으로 LVQ는 음(negative)의 학습을 하기 때문에 초기 가중치가 제대로 설정되지 않으면 발산할 수 있다는 단점이 있으며, 경쟁학습 계열의 신경망이기 때문에 출력 층의 뉴런 수에 따라 성능에 큰 영향을 받는다고 알려져 있다.[1]. 지도학습 형태를 지닌 LVQ의 경우에 학습패턴이 n개의 클래스를 가지고, 각 클래스 별로 학습패턴의 수가 같은 경우에 일반적으로 전체 출력뉴런에 대해서 (출력뉴런수/n)개의 뉴런을 각 클래스의 목표(desired) 클러스터로 할당하여 학습을 수행하는데, 본 논문에서는 각 클래스에 동일한 수의 출력뉴런을 할당하지 않고, 학습데이터에서 각 클래스의 분산을 추정하여 각 클래스의 분산을 추정분산에 비례하게 목표 출력뉴런을 할당하고, 초기 가중치도 추정분산에 비례하게 각 클래스의 초기 임의 위치 입력백터를 사용하여 학습을 수행하는 방법을 제안한다. 본 논문에서 제안하는 방법은 분류하고자 하는 데이터에 대해서 필요한 최적의 출력뉴런 수를 찾는 것이 아니라 이미 결정되어 있는 출력뉴런 수에 대해서 각 클래스에 할당할 출력 뉴런 수를 데이터의 추정분산에 의해서 결정하는 것으로, 추정분산이 크면 상대적으로 많은 출력 뉴런을 할당하고 작으면 상대적으로 적은 출력뉴런을 할당하고 초기 가중치도 마찬가지 방법으로 결정하며, 이렇게 하면 정해진 출력뉴런 개수 안에서 각 클래스 별로 분류의 어려움에 따라서 출력뉴런을 할당하기 때문에 미학습 뉴런이 줄어들게 되어 성능의 향상을 기대할 수 있으며, 실험적으로 제안된 방법이 더 나은 성능을 보임을 확인했다.initially they expected a more practical program about planting than programs that teach community design. Many people are active in their own towns to create better environments and communities. The network system "Alpha Green-Net" is functional to support graduates of the course. In the future these educational programs for citizens will becomes very important. Other cities are starting to have their own progrms, but they are still very short term. "Alpha Green-Net" is in the process of growing. Many members are very keen to develop their own abilities. In the future these NPOs should become independent. To help these NPOs become independent and active the educational programs should consider and teach about how to do this more in the future.단하였는데 그 결과, 좌측 촉각엽에서 제4형의 신경연접이 퇴행성 변화를 나타내었다. 그러므로 촉각의 지각신경세포는 뇌의 같은 족 촉각엽에 뻗어와 제4형 신경연접을 형성한다고 결론되었다.$/ 값이 210 $\mu\textrm{g}$/$m\ell$로서 효과적인 저해 활성을 나타내었다 따라서, 본 연구에서 빈

• Geophysics and Geophysical Exploration
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• v.3 no.2
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• pp.48-52
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• 2000

We have calculated and analyzed the electromagnetic responses of buried conductive pipes due to a horizontal magnetic dipole source on the pound using a three-dimensional (3-D) finite element method to provide useful guidelines for designing electromagnetic pipe locator and for field operation of the system. For single buried pipe, the horizontal component and the horizontal difference of the vertical component of magnetic field show peaks above the pipe. When comparing the width of response curves of both cases around the peak, horizontal difference of vertical component of magnetic field shows much narrower peak, 2 times narrower at a half of maximum amplitude, than that of horizontal component of magnetic field. Accordingly, we can pinpoint the horizontal location of pipe on the ground more accurately by measuring the horizontal difference of vertical component of magnetic fold. Moreover, it will have a merit in determining the depth of pipe, because the equation for depth estimation is defined just above the pipe. When there are two buried pipes separated by two meters with each other, the response of horizontal difference of vertical component of magnetic field has two separate peaks each of which is located above the pipe whereas horizontal magnetic field response has only one peak above the pipe just below the transmitter. Thus, when there exist more than a buried pipe, measuring the horizontal difference of vertical magnetic field can effectively detect not only the pipe under transmitter but also adjacent ones. The width of response curves also indicates higher resolving ability of horizontal difference of vertical component of magnetic field.

• Proceedings of the Korea Association of Information Systems Conference
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• 2004.11a
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• pp.349-365
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• 2004

기업의 또는 부문에서 구축된 각 정보시스템들은 시간이 지남에 따라 기업의 경쟁우위를 확보하기 위해 통합의 필요성이 증대하게 되었는데, 이러한 필요에 의해 제기된 것이 ERP(Enterprise Resource Planning: 전사적 자원관리)이다. 급변하는 기업의 내${\cdot}$외부 환경에 대해 신속하게 대응하기 위해 ERP 시스템을 도입하는 기업들의 수가 증가함에도 불구하고 ERP 원가관리시스템에 대한 연구는 아직까지 그 범위가 한정되었다고 할 수 있다. 본 논문은 ERP 도입 현황 및 원가관리시스템의 도입${\cdot}$설계현황자료를 바탕으로 하여, 중소기업의 ERP 원가관리시스템의 성공적 도입을 위한 방향을 제시하고자 한다. 중소기업에서 ERP 원가관리시스템을 도입할 때는, 원가관리시스템의 특성과 구축 목표, 구조 설계, 원가대상 설정 등과 관련된 다음과 같은 사항들을 고려하여야 성공적인 시스템을 구축할 수 있을 것이다. 첫째, ERP 원가관리시스템 특성 분석단계에서는 원가정보를 구성하는 내용의 충실성뿐만 아니라 정보가 전달${\cdot}$제공되는 범위와 대상의 적합성과 함께 그 표현의 형식 또한 고려되어야 한다. 둘째, ERP 원가관리시스템 구축목표 설정단계에서는 인가관리정보의 산출요건에 대한 명확한 이해와 목표설정을 기반으로 해야 한다. 셋째, ERP 원가 관리시스템 구조 분석 및 선계단계에서는 생산관리시스템 및 원가대상 설정 분석이 이루어져야 한다. 넷째, ERP 원가관리시스템 구현단계에서는 원가관리시스템과 타 계열시스템과의 인터페이스를 고려해야 한다. 따라서 원가관리시스템의 구현 시에는 관련시스템에서 어떠한 정보론 인터페이스 받을 것인가를 명확히 하여 시스템 가동 시에 타 관련시스템과 원활한 연계가 되도록 함으로써 전사적 종합시스템이 되도록 하여야 학 것이다.RS와 제진장치에 대한 전체적인 성능평가를 성공적으로 수행하였으며, 운전결과 및 경험은 향후 상용설비를 위한 기본자료로 활용할 것이다.X>, 그리고 입원기간은 $21.6\pm14.3일(13\~56)$이었다. 수술 후 평균 CK-MB는 $11.3\pm14.1ng/mL$였다. 수술 후 조기 혈관 개존율은 $100\% (24/24)$였다. 모든 환자에서 완전 추적이 가능하였으며 평균 추적기간은 $20.4\pm15.2개월(5\~43)$이었다. 이 기간 중 사망환자나 흉통이 재발한 환자는 없었다. 걸론: 80세 이상 고령의 환자에서 OPCAB은 수술 후 합병증을 줄이고 좋은 결과를 보여 주었다. 그러므로 고령의 환자에서도 관상동맥우회술의 적응증이 되면 적극적으로 수술을 시행할 필요가 있으며, 수술방법은 OPCAB이 좋을 것으로 생각한다서 실용적 개발의 가능성을 보였다.에 따라 현저한 차이가 있었으며 Dimethoate처리$(30^{\circ}C,\; 0.2\%$액에서 24시간)에 의하여 볍씨의 호흡량이 감소되었다. 9) 산소호흡량과 평균발아소요일수와는 $\gamma=-0.945$로 부의 유의한 상관을 보였는데 산소호흡량이 많은 품종은 평균발아소요일수가 짧은 경향을 보였다. 10) 볍씨의 산소호흡량과 Dimethoate 처리에 의한 볍씨의 발아저해도와는 $\gamma=-0,771$의 높은 부의 상관을 보였으며 산색호흡량이 많은 품종이 발아저해도가 낮고 적은 품종에서는 높았다. 현재까지는 그 활동이 11.2년의 주기성을 보여주지만 그 이전에 있어서는 그 활동이 극히 약화되었을 뿐만 아니라 매우 불규칙하다는 것이 Schneider와 Mass(1975)에 의해 밝혀졌다. 결국 1710년대부터 현재까지 우리나라에 있어서 벼멸구와 흰

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2011.06a
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• pp.27-28
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• 2011

A solar flares have the 11-year cycle and release a large energy which may produce coronal mass ejections (CME). The NOAA (National Oceanic and Atmospheric Administration) predicted that the sun spot activity will be maximized in 2013-2014. A strong solar flare can cause the disturbance of global positioning system including various communication of TV, radio broadcasting. The actual solar storm in 1989 caused power outages in Canada during 9 hours and about 600 million people had experienced a blackout. Such a solar storm can shorten the GPS satellite's life span about 5 to 10 years which can be resulted in economic loss considering the amount of multi-billion won. This paper analyzed the recent solar storm of X-class occurred on 15th of February about 10:45 this year that was reached Korea (Bohyun observatory) on 18th of February about 10:30 (local time), and compared with the data of before and after a week. The proton data of 18th of February considered that the solar strom reached on earth showed a fluctuation compared to the data of before and after a week. The positioning results at Daejeon also showed higher positioning error compared to the data of before and after a week results.