• Title/Summary/Keyword: mPer2

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Effect of mPER1 on the Expression of HSP105 Gene in the Mouse SCN

  • Kim Han-Gyu;Bae Ki-Ho
    • Biomedical Science Letters
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    • v.12 no.1
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    • pp.53-56
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    • 2006
  • The suprachiasmatic nucleus (SCN) of the anterior hypothalamus is the circadian pacemaker entrained to the 24-hr day by environmental time cues. Major circadian genes such as mPeriod ($mPer1{\sim}3$) and mCryptochrome ($mCry1{\sim}2$) are actively transcribed by the action of CLOCK/BMAL heterodimers, and in turn, these are being suppressed by the mPER/mCRY complex. In the study, the locomotor activity rhythms of mPer1 Knockout (KO) mice are measured, and the expression profiles of Heat Shock Protein 105kDa (HSP 105) genes in the SCN were measured by in situ hybridization. In agreement with previous reports, the locomotor activity rhythm of mPer1 KO mice was much shorter than that of wildtype. In addition, the total bout of activity of mPer1 KO was less in comparison to control mice. The expression of HSP 105 in the SCN of mPer1 KO mice was ranged from CT6 to CT22, with a peak level at CT14, implying that the gene are under the control of circadian clock. However, the expression of HSP 105 in the SCN of wildtype could not be detected in our study. Further analysis will reveal the direct or indirect regulation by mPer1 on the expression in the SCN and the role of the gene in the circadian clock.

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Comparison of yield and its components in spring sown wheat and barley by path coefficient analysis

  • Choi, In-Bae;Kim, Hak-Sin;Hwang, Jae-Bok;Bae, Hui-Su;Ku, Bon-Il;Park, Hong-Kyu;Par, Tae-Seon;Lee, Geon-Hwi
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.234-234
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    • 2017
  • Recent abnormal weather, especially continued rainfall during sowing season causes difficulty in proper sowing of wheat and delayed sowing after November 15 is concerned about freezing damage during winter, resulting in reduction of wheat yield. To correspond government policy of crop sufficiency improvement and produce and supply raw wheat and barley steadily, expansion of cultivation area is necessary and spring sowing of wheat is required. To obtain basic information on the improvement of spring sown wheat and barley production, comparison and path coefficients analysis was conducted for yield and yield related components from autumn and spring sown wheat and barley. Path analyses were known as very useful in clarifying the effects of yield components on grain yield formation, which were not accurately reflected in simple correlation anaylses. Most cultivated 5 wheat and 9 barley cultivars were sown on October and February at Cheon-ju province according to standard sowing method. For the spring sowing of wheat and barley, the varieties having vernalization degree I~III are seeded in the mid of February and seeding rate is 200~250kg/ha which is increased by 25% than autumn sowing. N-fertilizer of 95 kg/ha and the same amount of P, K dressed in autumn are applied at once as basal fertilizer. The magnitude of direct effect in each yield components on yield was in sequence as follows. In autumn wheat, grain number per $spike{\geq}$ the number of spike per $m^2$>1000-grain weight and in spring wheat, grain number per $ spike{\geq}the$ number of spike per $m^2$> 1000-grain weight. In autumn naked barley, 1000-grain weight> the number of spike per $m^2$, grain number per spike and in spring barely, the number of spike per $m^2$> grain number per spike > 1000-grain weight. In autumn covered barley, grain number per spike>the number of spike per $m^2$ and in spring coverd barley, the number of spike per $m^2$> grain number per spike, 1000-grain weight. In autumn malt barley, the number of spike per $m^2$>1000-grain weight and in spring malt barley, the direct effects of three yield components were similar. According to the path analysis of yield components for spring sown wheat and barley, it was suggested that adequate number of spike per $m^2$ was most important factor for yield increase.

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Effects of Number of Seedling per Hill and Plant Density on Rice Yield Components and Yield in the Late Season Culture of Rice (수도 만식재배에 있어서 재식밀도가 수량구성요소 및 수량에 미치는 영향)

  • In-Bea Kim;Joo-Yeol Lee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.24 no.2
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    • pp.57-63
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    • 1979
  • This study has been carried out to find out the optimum plant density and number of seedling per hill in the late season culture of rice at Chung Buk. P.O.R.D. Up to the level of 33 hills per m2 and 6 number of seedling per hill, rice yield was increased as the number of seedling per hill and plant density per unit areas increased. However it is recommendable to have a plant density of 28~33 hills per m$^2$ with 4~6 number of seedling per hill for the late season culture.

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A Study on the Parking Demand of Apartment Complex in Daegu city (대구시 아파트단지의 주차수요에 관한 연구)

  • Park Chan-Don;Ha Jae-Myung
    • Journal of the Korean housing association
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    • v.15 no.5
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    • pp.51-58
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    • 2004
  • After 1980's, the ratio of car possessions has been increased rapidly. Nowaday, most large cities have suffered a lot of problems about traffic and car parking. Specially in apartment complexes, they have parking problem which is caused by the parking demand. The ratio of parking demand at multi-family housing sites has been increased significantly, therefore several parking problems have occurred. The goal of this study is to investigate the parking demand per housing unit size of apartment complex in Daegu city. The results of this study is as follows. (1) The parking demand of 60 $m^2$ below sized housing unit is 1.09 car per the unit. (2) The parking demand of 60 $m^2$ over 85 $m^2$ below sized housing unit is 1.31 car per the unit. (3) The parking demand of 85 $m^2$ over 135 $m^2$ below sized housing unit is 1.74 car per the unit. (4) The parking demand of 135 $m^2$ over sized housing unit is 2.10 car per the unit.

Proteomic Analysis of Circadian Clock Mutant Mice

  • Lee Joon-Woo;Kim Han-Gyu;Bae Kiho
    • Biomedical Science Letters
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    • v.11 no.4
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    • pp.493-501
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    • 2005
  • Circadian rhythms, time on a scale of about 24 hours, are present in a number of organisms including animals, plants, and bacteria. The control of the biochemical, physiological and behavioral processes is regulated by endogenous clocks in the suprachiasmatic nucleus (SCN). At the core of this timing mechanism is molecular machinery that are present both in the brain and in the peripheral tissues throughout the body, and even in a single cultured cell. In this study, we performed two-dimensional gel electrophoresis to figure out any correlation between protein expression patterns and the requirement of two canonical clock proteins, either mPER1 or mPER2, by comparing global protein expression profiles in livers from wildtype or mPer1/mPer2 double mutant mice. We could identify several differentially expressed protein candidates with respect to time and genotypes. Further analysis of these candidate proteins in detail in vivo will lead us to the better understanding of how circadian clock functions in mammals.

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Bacteriological Contamination of Water in Han River basin (한강유역의 수중미생물 오염도 조사)

  • Choi, Han-Young;Park, Chung-Oh
    • Journal of environmental and Sanitary engineering
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    • v.4 no.2 s.7
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    • pp.47-59
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    • 1989
  • In order to investigate the bacteriological contamination of water in Han river, the survey was carried out in eight reservoirs of Seoul water supply during the period from January to December in 1985. 1. The counts by means of total bacteria in eight reservoirs by standard plate count method were as follows: $7.7\times10^2$ per ml in Paldang reservior, $9.6\times10^3$ per ml in Gueiri, $8.4\times10^4$ per ml in Doogdo, $1.6\times10^6$ per ml in Bogwang, $2.5\times10^6$ per ml in Noryangjin, $2.2\times10^6$per ml in Seon yoo, $5.9\times10^6$ per ml in Yungdeungpo and $1.9\times10^7$per ml in Gayang. 2. The average counts of total coliform in eight reservoirs by MPN method were as follows : $2.4\times10$ per 100 ml in Paldang, $5.6\times10^2$ per 100 ml in Gueiri, $2.3\times10^3$ per 100 ml in Doogdo, $5.1\times10^4$ per 100 ml in Noryang-jin, $1.2\times10^5$ per 100 ml in Bogwang, $6.2\times10^4$ per 100 ml in Seonyoo, $1.1\times10^5$ per 100 ml in Yungdeungpo and $2.8\times10^5$ per 100 ml Gayang. 3. The counts by means of fecal coliform in eight reservoirs by MPN method were as follows : non detection per 100 ml in Paldang, 5.2 per 100 ml in Gueiri, $1.2\times10^2$ per 100 ml in Doogdo, $1.6\times10^3$ per 100ml in Bogwang, $2.0\times10^3$per 100ml in Noryangjin, $6.6\times10^2$ per 100ml in Seonyoo, $1.2\times10^3$ per 100 ml in Yungdeungpo and $2.5\times10^3$per 100 ml in Gayang. 4. The counts by means of fecal streptococci in eight reservoirs by MPN method were as follows: non detection per 100 ml in Paldang and Gueiri, $6.9\times10$ per 100 ml in Doogdo, $3.2\times10^2$ 102 per 100 ml in Bogwang, $2.9\times10^2$ per 100 ml in Noryangjin, $3.0\times10^2$ per 100 ml in Seonyoo, $4.0\times10^2$ per 100 ml in Yungdeungpo and $14\times10^3$ per 100 ml in Gayang. 5. The counts means of pseudomonas aeruginosa in eight reservoirs by MPN method were as follows; non detection per 100 ml in Paldang, 2.4 per 100 ml in Gueiri, $1.5\times10$ per 100 ml in Doogdo, $2.0\times10$ per 100ml in Bogwang,$6.2\times10$ per 100mI in Noryangjin, $2.1\times10$ per 100ml in Seonyoo, $6.4\times10$ per 100mI in Yungdeungpo and $7.1\times10$ per 100ml in Gaynag.

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Population Ecological Characteristics of the Soft-shelled Clam, Mya japonica in the Intertidal Zone of South Sea in Korea (한국 남해안 조간대에 서식하는 우럭, Mya japonica의 자원생태학적 특성)

  • 이선길;장창익
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.36 no.3
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    • pp.234-243
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    • 2000
  • This paper is to study population ecological characteristics, including growth parameters, survival rate, instantaneous coefficients of natural and fishing mortalities, and age at first capture of the soft-shelled clam, Mya japonioa in the intertidal zone of South Sea in Korea. For describing growth of the clam a von Bertalanffy growth model was adopted, The von Bertalanffy growth curve had an additive error structure and the growth parameters estimated from a non-linear regression were SH/sub ∞/=79.83mm, K=0.26, and t/sub 0/= -0.01. Survival rate (S) of the soft-shelled clam was 0.26 (SD=0.02). The instantaneous coefficients of natural mortality (M) was estimated to be 0.78/year and fishing mortality (F) 0.57/year for the soft-shelled clam. The age at first capture (t/sub c/) was estimated as 2.69 year. The mean densities of the soft-shelled clam by bottom type were 3.40 inds./m²(SE=0.18) in the sand, 63.4 inds./m²(SE= 0.53) in the muddy sand, and 0 inds./m2 (SE=0) in the gravelly sand. The mean densities of the soft-shelled clam by 3 different areas were 4.88 inds./m²(SE=0.09), 2.61 inds./m²(SE=0.13), 7.20 inds./m²(SE=0.18), respectively and the biomass of the clam were estimated as 131mt, 121mt, 665mt, respectively. An yield-per-recruit analysis showed that the current yield-per-recruit of about 8.30g with F=0.57/year and the age at first capture (t/sub c/) 2.69 year, was lower than the maximum possible yield-per-recruit of 9.60g. Fixing to at the current level and increased fishing intensity (F) could produce an increase in the predicted yield-per-recruit from 8.30g to about 9.40. However, estimated yield-per-recruit increased to 1.30g by decreasing to from the current age (2.69 year) to age two with F fixed at the current level. Yield-per-recruit was estimated under harvest strategies based on F/sub max/ and F/sub 0.1/.

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The Calculation of NPS Load per Unit Area in Orchard to the Nakdong River Basin (낙동강유역 과수재배지의 단위면적당 비점오염부하량 산정에 관한 연구)

  • Lee, Jae-Woon;Kwon, Heon-Gak;Yi, Youn-Jeong;Cheon, Se-Uk
    • Journal of Environmental Impact Assessment
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    • v.22 no.6
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    • pp.557-568
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    • 2013
  • In this study, Calculated the nonpoint sources(NPS) load per unit area about various rainy events in vineyard of Nakdong River basin. NPS monitoring and calculation for NPS load per unit area were estimated from 'Investigation method of precipitation discharge(National Institute of Environmental Research, 2007)'. The evaluation of applicability for NPS load per unit by compared with prior research data and Total Maximum Daily Load(TMDL) data. Five target areas were each $2000m^2$, $1800m^2$, $1943m^2$, $2484m^2$, $864m^2$ and located in Gyeongsangbukdo Gyeongju, Gyeongsangbukdo Sangju, Gyeongsangnamdo Hapcheon in Korea. Since fruits were the only crop on the target area, the characteristics of stormwater discharge at survey sites could be evaluated independently. A total of 115 rainfall events in the Orchard area during five years(2008-2012) was surveyed, and 38 of them became stormwater discharge. In the Nakdong River watershed, average of event mean concentrations(EMCs) in Orchard area for biochemical oxyzen demand(BOD), Chemical oxyzen demand(COD), total nitrogen(T-N), total phosphorus(T-P) were 2.0mg/L, 10.1mg/L, 3.195mg/L, 0.578mg/L, respectively. NPS load per unit area in Orchard area showed BOD : $2.0kg/km^2{\cdot}day$, COD : $10.2kg/km^2{\cdot}day$, T-N : $3.220kg/km^2{\cdot}day$, T-P : $0.606kg/km^2{\cdot}day$.

Effect of Number of Sowing Grain on the Rice Growth and Yield in Direct Sowing Culture on Irrigated Paddy Field (벼담수 직파 재배에서 파종 입수가 생육 및 수량에 미치는 영향)

  • Jong-Chul Lee;Chang-Sik Moon;Hae-Yeong Suh;Beom-Yeol Choi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.14
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    • pp.41-45
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    • 1973
  • This study was carried out in the central part of Korea in 1972 to investigate optimum grain number per square meter and variation of the yield and its components in the direct sowing culture on irrigated paddy field. The results could be summarized as follows; 1. Number of maximum tillerings was directly increased with increase of grain number from 120 to 280 per square meter and it was gradually decreased with increase of number sowing grain. So number of maximum tillerings showed at the plot of 280 grain per square meter. Also it was showed remarkable competition at the plot more than 280 grain per square meter. 2. Percentage of effective tiller was decreased with increase of number of sowing grain from 120 to 280 per square meter and after it was gradually decreased with increase of number of sowing grain. 3. It was not remarkable competition in plot less than 1, 000 tillering per square meter on the number of maximum tillerings. 4. It was remarkable positive correlation between the number of panicle and the number of sowing grain, but it was tendency to negative correlation between the number of panicle and the number of spiklets per panicle, and between the number of panicle and the ripening ratio. 5. Correlation coefficient between the yield and the number of spiklet per square meter of land area was 0.929, r=0.695 in the yield and the number of panicle, r=0.796 in the number of panicles and the number of spiklets per square meter Also in rank correlation, it was showed most highly positive correlation(r=0.954) between the yield and the number of panicles, so number of panicles was most largely dominated on yield among the yield components in direct sowing culture on irrigated paddy field. 6. It was producted highest yield in the plot of 280 grain sowing per square meter.

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Optimum Crop Load in Different Planting Densities of Adult 'Fuji'/M.9 Apple Tree for Preventing Biennial Bearing and Stabilizing Tree Vigor (성목기 '후지'/M.9 사과나무의 해거리 방지와 수세안정을 위한 재식거리별 적정 착과 수준)

  • Sagong, Dong-Hoon;Yoon, Tae-Myung
    • Horticultural Science & Technology
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    • v.33 no.1
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    • pp.1-10
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    • 2015
  • This study was conducted in three years (7-9 years after planting) to investigate vegetative growth, yield, fruit quality, and return bloom for optimum crop load based on different planting densities of adult 'Fuji'/M.9 apple trees. As plant materials, 'Fuji'/M.9 apple trees planted at $3.5{\times}1.5m$ (190 trees per 10 a), $3.5{\times}1.2m$ (238 trees per 10 a), and $3.2{\times}1.2m$ (260 trees per 10 a) spacing and trained as slender spindles were used. The crop load was assigned to five different object ranges as follows: 55-64, 65-74, 75-84, 85-94, and 95-104 fruit per tree. TCA increment, total shoot growth, return bloom, yield per tree, and yield efficiency tended to increase as planting density decreased, and fruit weight and soluble solid content tended to increase as the object range of crop load decreased. Fruit red color tended to increase as shoot growth decreased. For apple trees planted with 238 trees and 260 trees per 10a, biennial bearing occurred when the crop load was over 85-94 and 75-84 fruits, respectively. However, biennial bearing did not occur when the crop load was 95-104 fruits in apple trees planted with 190 trees per 10a. Accumulated yield tended to increase as planting density and crop load increased, but that of biennial bearing did not show such a difference. Based on our results, optimum crop load recommendations are to set 95-104 fruits per tree in 'Fuji'/M.9 mature apple trees planted at 190 trees per 10a, 75-84 fruits per tree at 238 trees per 10a, and 65-74 fruits per tree at 260 trees per 10a.