• BMB Reports
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• v.40 no.6
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• pp.911-920
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• 2007

Tuberculosis, caused by Mycobacterium tuberculosis, continues to be one of the leading infectious diseases to humans. It is urgent to discover novel drug targets for the development of antitubercular agents. The 2-C-methyl-Derythritol-4-phosphate (MEP) pathway for isoprenoid biosynthesis has been considered as an attractive target for the discovery of novel antibiotics for its essentiality in bacteria and absence in mammals. MEP cytidyltransferase (IspD), the third-step enzyme of the pathway, catalyzes MEP and CTP to form 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) and PPi. In the work, ispD gene from M. tuberculosis H37Rv (MtIspD) was cloned and expressed. With N-terminal fusion of a histidine-tagged sequence, MtIspD could be purified to homogeneity by one-step nickel affinity chromatography. MtIspD exists as a homodimer with an apparent molecular mass of 52 kDa. Enzyme property analysis revealed that MtIspD has high specificity for pyrimidine bases and narrow divalent cation requirements, with maximal activity found in the presence of CTP and $Mg^{2+}$. The turnover number of MtIspD is $3.4 s^{-1}$. The Km for MEP and CTP are 43 and $92{\mu}M$, respectively. Furthermore, MtIspD shows thermal instable above $50^{\circ}C$. Circular dichroism spectra revealed that the alteration of tertiary conformation is closely related with sharp loss of enzyme activity at higher temperature. This study is expected to help better understand the features of IspD and provide useful information for the development of novel antibiotics to treat M. tuberculosis.

• Journal of Korean Society of Forest Science
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• v.84 no.2
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• pp.247-257
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• 1995

This research carried out forest community classification on the basis of the methods of ZM school, in order to furnish useful information needed for forest management, and for afforestation and preservations of Abies koreana forest which was Korean native species, and the research results could be summarized as follows. Abies koreana forest was divided into 10 vegetation units ; This forest was divided into Abies koreana-Quercus mongolica community and Abies koreana-Sasa quelpartensis community. Abies koreana-Quercus mongolica community was divided into Tripterygium regelii group, Patrinia saniculaefolia group, and Typical group, and Tripterygium regelii group seas divided into Typical subgroup. Pimpinella brachycarpa subgroup, and Hemirocallis fulva subgroup. Abies koreana-Sasa quelpartensis community was divided into Berberis koreana group, Trachelospermum asiaticum var. intermedium group, and Typical group. Berberis koreana group was divided into Typical subgroup and Hepatica asiatica subgroup, and Tranchelospermum asiaticum var. intermedium group was divided into Hepatica asiatica subgroup and Typical subgroup. According to the results of the analysis by coincidence method, Abies koreana-Quercus mongolica community represented in Mt. Chiri, Mt. Deokyu and Mt. Kaya which were located in the Sobaek mountains, Abies koreana-Sasa quelpartensis community represented in Mt. Hanla. Therefore it was thought that Abies koreana forest was classified by geographical position, and vegetation units of Abies koreana forest tended to be classified by the altitude and topography. As for DBH and height of Abies koreana, mean DBH and height of Abies koreana-Quercus mongolica community were each 28.4cm, 10.6m that were larger than mean DBH and mean height(each 23.6cm, 6.3m) of Sasa quelpartensis community. Among Abies koreana-Quercus mongolica community, Patrinia saniculae-folia group had the smallest mean DBH and mean height(20cm, 5m), and among Abies koreana-Sasa quelpartensis community, Typical group had the smallest mean DBH and mean height(4cm, 3m).

• Journal of Animal Environmental Science
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• v.6 no.1
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• pp.53-58
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• 2000

A manure management plan is important for all daily operations. This study was conducted to investigate the effect of application level of liquid cattle manure on the soil properties and changes of BOD and COD content in run-off water at the mixtures swards of National Livestock Research Institute RDA Suweon in 1995. The experiment was arranged in a randomized block design with five treatments consisting of no fertilizer chemical fertilizer application of 40, 60 and 80MT/ha as liquid cattle manure. The quantity of run-off for a no fertilizer was the highest of 1,469.4mm but that for a application level of liquid cattle manure of 60MT/ha was the lowest of 1,278.1mm. The change of BOD in run-off for a no fertilizer was the lowest of $19.84m{\ell}/{\;}{\ell}$, but that for a application level of liquid cattle manure of 80MT/ha was the highest of $36.22m{\ell}/{\;}{\ell}$. Change of COD in run-off for a no fertilizer was the lowest of $21.28m{\ell}/{\;}{\ell}$ but that for a application level of liquid cattle manure of 80MT/ha was the highest of $37.51m{\ell}/{\;}{\ell}$. Available phosphorus and total-N content of soil chemical properties was higher at liquid cattle manure than chemical fertilizer.

• Journal of the Korean Geosynthetics Society
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• v.12 no.3
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• pp.65-73
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• 2013

The information of cut slope in Mt. Jang area, Busan is investigated in order to construct the Slope Management System in Urban Area. The slope inspection sheet is made to record the characteristics for cut slopes, and that is capable to be inputted slope information systematically. The cut slopes in Mt. Jang area are consisting of 69 slopes. Most of the cut slopes are constructed in cutting slope and retaining wall (CR). The cut slopes located in housing facilities are 46 slopes, and the slopes located in school facilities are 12 slopes. The traverse of cut slopes is mainly ranged from 50 m to 150 m, and the height is mainly ranged from 10 m to 20 m. The slopes combined with soil and rock are mostly distributed. The retaining wall was installed in the toe part of cut slope in order to increase the slope stability, and the additional reinforcement methods including the anchor, drainage, preventing rock fall, shotcrete and vegetation were installed at the toe part of cut slopes.

• Journal of Life Science
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• v.29 no.7
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• pp.762-768
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• 2019

Doellingeria scabra Thunb. (syn. Aster scaber Thunb.), a perennial herb in the family Asteraceae, is frequently found in the wild mountain regions of Korea. This aim of this work was to measure the characteristics of patchiness of D. scabra in a local population on Mt. Maebong in Taeback-ci, Gangwon-do. The spatial distribution pattern of this species was estimated by analyzing ecological data by methods including the index of dispersion, Lloyd's mean crowding, and Morisita's index. The mean population density of the D. scabra population was 2.94. The D. scabra individuals were uniformly or randomly distributed in small-scale plots and were aggregately distributed in two large-scale plots ($16{\times}32m^2$ and $32{\times}32m^2$). The mean crowding ($M^*$) was 0.916. The mean patchiness index (PAI) was 0.796. Morisita's coefficient tended to decrease the density of the population as the plot size increased. The expected value of Eberhardt's index ($I_E$) in the local population was 2.623. Moran's I of D. scabra significantly differed from the expected value in 6 of 8 cases (75.0%). The first five classes were positive, with four showing statistical significance, indicating similarity among individuals in the first four distance classes (I-IV, 8 m), The results presented here could provide a theoretical basis for the conservation of D. scabra (Korean: chamchwi) and for the rehabilitation and sustainable management of forest ecosystems on Mt. Maebong, as well as on other mountains.

• Korean Journal of Environmental Agriculture
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• v.35 no.2
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• pp.137-142
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• 2016

BACKGROUND: Identification of endophytic yeasts inhabiting the internal roots of the Mankyua chejuense tree requires techniques involving biotechnology. There is a need for a culture-based method to isolate and identify yeast strains associated with M. chejuense.METHODS AND RESULTS: We spread homogenized M. chejuense root samples onto glucose-peptone- yeast agar containing antibiotics, Triton X-100, and L-sorbose. A total of 152 yeast isolates were obtained and identified via phylogenetic analysis based on ITS gene sequencing. The results revealed that the root-associated yeast species included the genera Cyberlindnera (140 isolates), Candida (11 isolates), and Kluyveromyces (one isolate). Additionally, three yeast isolates showed high bioethanol production.CONCLUSION: We identified the specific yeast community associated with M. chejuense roots. These yeast isolates may have industrial applications as bioethanol producers. Our findings revealed that Cyberlindnera isolates included C. suaverolens and C. satumus, while Kluyveromyces isolates showed high bioethanol production.

• Microbiology and Biotechnology Letters
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• v.18 no.4
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• pp.433-436
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• 1990

The TOL plasmid, pWWO, conjugally transferred from Pseudomonas putida mt-2 was dissociated into TOL* and TOL $\Delta$A in P. putidu PpGl carrying CAM plasmid. The TOL* was integrated into the CAM plasmid, and the resulting plasmid was designated as CAM::TOL*. The introduction of NAH plasmid, belonging to Inc P9 incompatibility group, into P. putida CSTBA carrying CAM::TOLt plasmid and TOL A plasmid did not affect m-toluate catabolism, but resulted in expelling the TOL $\Delta$ plasmid.

• Archives of Pharmacal Research
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• v.29 no.11
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• pp.957-962
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• 2006

A novel series of 2-(m-Chlorobenzyl)-4-substituted-1, 1, 3-trioxo-2H, 4H-pyrazolo[4, 5-e][1, 2, 4] thiadiazines (7a-k) were synthesized, and evaluated for their anti-HIV replication in MT-4 cell cultures. Compound (7a) showed activity against HIV-1-induced cytopathicity, with an $EC_{50}$ value of $45.6\;{\mu}M$, but none of the compounds exhibited inhibitory activity against HIV-2.

• Korean Journal of Environment and Ecology
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• v.35 no.5
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• pp.438-479
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• 2021

Basic information such as flora and their distribution are the key factors to consider for preserving plant diversity on a regional level. The number of plant species identified through a survey is closely related to the survey area and the number of habitats distributed within the survey area. In this study, we surveyed the flora of Mt. Maryong-san (PH) and Mt. Daeso-san (YH) located in Uljin-gun, Gyeongsangbuk-do, six and nine times, respectively. We planned to include a variety of habitats in the survey routes. As a result, we identified a total of 594 taxa composing of 112 families, 349 genera, 548 species, 10 subspecies, 34 varieties, and 2 formulas in the Mt. Maryong-san (PH) area and a total of 434 taxa composing of 89 families, 270 genera, 400 species, 9 subspecies, and 25 varieties in the Mt. Daeso-san (YH) area. Of the plants observed in the 21 habitats, the plants distributed in the transition zone around the valley and the dry grassland accounted for the highest proportion. The traditional graveyards located on the coast also served as the major habitat for the distribution of some plant species. In the two graveyards, 19 taxa of endemic plant species were surveyed. Moreover, the identified floristic target species included 1 taxon for grade V, 11 taxa for grade IV, and 24 taxa for grade III. A total of 77 taxa of naturalized plants were identified, and four of them were invasive alien plants as designated by the Ministry of Environment. The differences in the plants distributed in the area of Mt. Maryong-san (PH) and Mt. Daeso-san (YH) were mainly due to the difference in the size of each habitat distributed within the survey area. Moreover, it was judged that the entire vicinity of Mt. Daeso-san (YH) was not connected to the surrounding mountains and was an isolated area surrounded by farmland and residential areas.

• The Journal of the Petrological Society of Korea
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• v.19 no.2
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• pp.109-121
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• 2010

Mt. Mudeung is located in Gwangju city, Damyang-Gun, Hwasun-Gun and its round form give us the mood of soft and rich. Its location is $126^{\circ}06'-127^{\circ}01'E$ and $35^{\circ}06'-35^{\circ}10'N$ and its highest peak is Cheonwang-bong with the height of 1,187 m. The Gwangju city is located in the West of Mt. Mudeng and the mountain range with a small basin in its East. The pavilion such as the Soswaewon, Songganjeong, Sigyongjeong are distributed along the stream in the north of Mt. Mudeung. The mountain is formed from the volcanic activity, Gwangju cauldron during the Cretaceous. The top part of Mt. Mudeung is composed of dark gray quartz-andesite and its K-Ar whole rock age is $48.1{\pm}1.7Ma$. The composition of the north area, where the Wonhyosa temple is located, is micrographic granite, whereas the composition of south area is rhyolite mainly. The main ridge of Mt. Mudeung runs from North, starting from the Bukbong, to south, passing Cheonwangbong, Jangbuljae and ending Anyangsan. Geologic feature of the mountain includes volcanic landform, mountaineous landform, and stream landform. The Seosukdae, Ipseokdae, Gyubongam, which are main ridges and formed from volcanic activity, are composed of mainly columnar joint. Saeinbong and Majipbong in the south-west are composed of mainly cliff and dome. The typical erosion landform of the mountain has three different types of the weathering-cave, each of which reflect the property of the original rock. Four different area of wide block stream, they makes the geological feature of spring-water, though its scale is small compared to that of water fall.