• 한국환경보건학회지
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• 제28권2호
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• pp.149-160
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• 2002

Analysis of benthic macroinvertebrate community structure and biological estimation of water quality by the benthic macroinvertebrates from the Mt. Myungji(1267m), Mt. Kalbong(840m), Mt. Daegeum(704m) and Mt. Jubal(489m) region of Gapyung-gun, Kyunggi-do were investigated from April to November, 2001. Total taxa of benthic macroinvertebrates were 105 species, 38 families, 13 orders, 5 classes in 4 phyla. Aquatic insects were 33 species in ephemeroptera, 5 species in odonata, 13 species in plecoptera, 2 species in hemiptera, 1 species in megaloptera, 27 species in trichoptera, 3 species in coleoptera and 13 species in diptera, respectively. Non-insects were 1 species in platyhelminthes, 5 species in gastropoda, 1 species in oligochaeta, 1 species in crustacea, respectively. Ephemeroptera, trichoptera and plecoptera as indicators in clean water were very abundantly. Occurrence species at each survey area was 67 species at Mt. Myungji and 69 species at Mt. Kalbong, 48 species at Mt. Daege.um and 47 species at Mt. Jubal, respectively. Mean species diversity indices at Mt. Myungji, Mt. Kalbong, Mt. Daegeum and Mt. Jubal were 3.30, 3.33, 2.59 and 3.43, respectively. According to the saprobic system based on the species diversity indices of benthic macroinvertebrates, Mt. Myungji, Mt. Kalbong and Mt. Jubal are determined as Limnosaprobic area, respectively, but Mt. Daegeum is determined as $\beta$-mesosaprobic area.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2000년도 제7차 국제 심포지움(생약자원개발에 관한연구) 및 추계정기 학술발표회 초록집
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• pp.60-64
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• 2000

This examination of vegetation is conducted from lune to Oct. 1999 through on the spot survey, centering on the habitat of Abies koreana around Imgeolryeong, langteomok, and Jeseokbong in Mt Jiri and Yeongsil path and Seongpanak path in Mt. Halla. Species composition tables of the Abies koreana community by altitude and slope in Mt. Jiri and Mt. Halla are made based on the community composition tables examined in quadrat. The Abies koreana-Saso quelpaertensis community in a west slope of Mt. Halla is found that Abies koreana of 4-6m in height forms subtree layer and that of 8-9m in height the tree layer and it reflects a difference between community structure by slope and main composition species. While the tree layer of Abies koreana community is 12-14 in height around the area of 1, 290-1, 560m above the sea of Imgeolryong and path from Baekmudong to Jangteomok, it is 8-12m in height in the area of 1, 680-1, 780m above the sea of path from Jangteomok to Jeseokbong. It means that community structure depends on area and attitude. This study finds out that commonly appearing composition species similar to coverage and presence are Lepisorus thunberianus, Quercus mongolica, Soidago virga-aurea var. asiatica, Ligularia fischeri, Carex lanceolata, Clintonia udensis, Magnolia sieboldii, Betula ermani, Acer Pseudo-sieboldianum, As soil environment of surveyed area, pH of surveyed spots is similar in Mt. Jiri and Mt. Halla, electrical conductvity is higher in Mt Jiri, and contents of organic matter is relatively higher in a west slope of Mt. Jiri and Mt. Halla and lower in their south and west slopes. Contents of organic matter and total nitrogen show that the area of 1, 550m above the sea in a west slope of Mt. Halla is some higher. For pH and contents of total nitrogen and organic matter, Mt. Halla is higher than Mt. Jiri but for electrical conductivity , Mt. Jiri is relatively higher than Mt. Halla.

• Plant Resources
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• 제3권2호
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• pp.138-147
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• 2000

This examination of vegetation is conducted from June to Oct. 1999 through on the spot survey, centering on the habitat of Abies koreana around Imgeolryeong, Jangteomok, and Jeseokbong in Mt Jiri and Yeongsil path and Seongpanak path in Mt. Halla. Species composition tables of the Abies koreana community by altitude and slope in Mt. Jiri and Mt. Halla are made based on the community composition tables examined in quadrat. The Abies koreana-Saso quelpaertensis community in a west slope of Mt. Halla is found that Abies koreana of 4-6m in height forms subtree layer and that of 8-9m in height the tree layer and it reflects a difference between community structure by slope and main composition species. While the tree layer of Abies koreana community is 12-14 in height around the area of 1,290-1,560m above the sea of Imgeolryong and path from Baekmudong to Jangteomok, it is 8-12m in height in the area of 1,680-1,780m above the sea of path from Jangteomok to Jeseokbong. It means that community structure depends on area and attitude. This study finds out that commonly appearing composition species similar to coverage and presence are Lepisorus thunberianus, Quercus mongolica, Solidago virga-aurea var. asiatica, Ligularia fischeri, Carex lanceolata, Clintonia udensis, Magnolia sieboldii, Betula ermani, Acer pseudo-sieboldianum. As soil environment of surveyed area, pH of surveyed spots is similar in Mt. Jiri and Mt. Halla, electrical conductivity is higher in Mt Jiri, and contents of organic matter is relatively higher in a west slope of Mt. Jiri and Mt. Halla and lower in their south and west slopes. Contents of organic matter and total nitrogen show that the area of 1,550m above the sea in a west slope of Mt. Halla is some higher. For pH and contents of total nitrogen and organic matter, Mt. Halla is higher than Mt. Jiri but for electrical conductivity, Mt. Jiri is relatively higher than Mt. Halla.

• 한국발생생물학회지:발생과생식
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• 제4권1호
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• pp.45-52
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• 2000

Membrane-type matrix metalloproteinase(MT-MMP)는 세포막에 부착된 채로 작용하는 단백질 가수분해효소로서 최근들어 정상 및 암세포 등 각종 조직세포의 재구성에 중요한 역할을 하는 것으로 알려지고 있다. 본 연구에서는 RT-PCR 방법을 사용하여 생쥐 난자와 초기배아에서의 MT1-MMP와 MT2-MMP 유전자 발현 양상을 조사하였다. 생후 3주 및 8주된 생쥐의 난소로부터 얻은 미성숙난자와 체외 및 체내에서 성숙시킨 난자에서의 MTl-MMP와 MT2-MMP mRMA의 발현을 조사하였다. 그 결과 미성숙난자와 체외 및 체내에서 성숙시킨 난자 모두에서 MT1-MMP 및 MT2-MMP의 mRNA가 발현되었으나 미성숙난자에서는 매우 약하게 발현되는 것이 관찰되었다. 2세포기 배아, 4세포기 배아, 상실배, 포배 및 탈각한 포배를 대상으로 MT2-MMP mRNA의 발현양상을 조사한 결과 2세포기에서는 발현이 일어나지 않았고 4세포기에 이르러서야 뚜렷한 발현이 관찰되었다. 상실배와 포배에서는 현저히 강한 발현이 관찰되었다. 생쥐의 난소조직에서도 MT1-과 MT2-MMP모두 발현되는 것이 관찰되었는데 각각은 배란을 전후로 한 시기에 상관없이 항상 같은 정도의 mRNA발현을 나타내었다. 생쥐의 수란관조직도 난소조직과 유사하게 배란시기에 상관없이 같은 수준의 MT1-과 MT2-MMP mRNA 발현양상을 보여주었다. 이러한 결과들로 미루어 생쥐 배아에서 발현되는 MT2-MMP는 초기배아의 분화과정에서 중요한 역할을 할 것으로 사료된다. 난소와 수란관의 조직재구성과 관련한 MT1-과 MT2-MMP의 역할은 앞으로 더 연구되어야 할 것이다.

• 한국동물학회지
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• 제31권3호
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• pp.218-224
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• 1988

Drosophila melanogaster의 세계 14지역 계통으로부터 mitochondrial DNA(mtDNA)를 추출하여, 제한 요소에 의하여 mtDNA종내 변이를 조사하였다. 그 결과, site variation(Hpall와 Haelll 및 Seal효소)과 length variation(최대550bp)이 나타났다. 또한 6종류(Ml, M2, M3, M4, M6 및 M7)의 mtDNA genotype이 검출되었으며, 종내 평균 염기 치환율은 1.88%로써 낮은 지역 분화(low divergence)를 나타내었다. 그러나 일본의 Ogasawara계통의 M5 type은 본 연구에서는 검출되지않았다. 이처럼 지역 계통간의 낮은 지역 분화는 세계14지역 계통의 D. melanogaster가 최근에 소수의 개체로부터 확산되었기 때문에 집단전체에 아직 충분한 mtDNA변이가 축적되지 않았거나 혹은 지리적 격리가 충분함에도 불구하고 지역 계통간에 빈번한 migration이 일어났기 때문에 mtDNA의 지역 분화가 방해되지않은 것으로 추측된다.

• Plant Resources
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• 제5권1호
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• pp.51-58
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• 2002

Many higher fungi were collected at Mt.Manrae, Mt.Kirin, Mt.Nam, Mt.Unjang, Mt.Yonsuk, Mt.Minjuji from May, 2000 to June, 2001 and they were identified. As the result, Marasmius buxi, M. epiphylloides, M. hudsoni, Mycena erubescens, Agaricus bisporus var. bisporus, Panellus ringens, Lactarius aquiflus, Leccinum quericinum were newly discovered in Korea. They were designed Korean common names by author.

• 한국어류학회지
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• 제23권1호
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• pp.61-69
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• 2011

멸종위기 어류 미호종개(Cobitis choii)로부터 중금속해독 단백질(metallothionein) 유전자를 분리, 클로닝하고 중금속 및 고온 스트레스에 대한 전사 발현 특정을 분석하였다. 미호종개 metallothionein는 gDNA, mRNA 및 아미노산 서열 모두에서 경골 어류 MT들의 구조적 특징을 잘 보전하고 있었으며, 생물정보분석을 통해 미호종개 MT 유전자 5'-upstream 영역은 중금속 조절, 면역 반응 및 온도 반응에 관여하는 다양한 전사 조절인자들의 부착 위치들을 포함하는 것으로 관찰되었다. 카드뮴(Cd), 구리(Cu), 니켈(Ni), 망간(Mn) 및 아연(Zn)을 이용한 침지 노출 실험(0.5 및 $1.0\;{\mu}M$; 24시간)에서 미호종개 MT mRNA 발현은 구리 및 카드뮴 처리군에서 가장 많이 유도되었고($1.0\;{\mu}M$ Cu 처리군에서 최대 10배), 망간 처리군에서는 비교적 적은 양의 MT 발현이 유도된 반면(2배), 아연 및 니켈 노출 군에서는 유의적인 MT 발현의 증감이 관찰되지 않았다. 또한 미호종개 MT 전사 발현은 고온 자극 ($25^{\circ}C$로부터 $31^{\circ}C$까지 증가)에도 민감하게 반응하는 것으로 나타나, $31^{\circ}C$ 도달시점에서 $25^{\circ}C$ 초기 MT mRNA 발현 수준보다 9배 높은 mRNA 발현이 관찰되었다. 본 연구 결과는 MT 기반의 유전자 발현 분석을 이용함으로써, 향후 멸종위기 어류 미호종개의 스트레스 반응 연구에 유용한 기초 자료를 제공할 수 있다고 기대된다.

• The Korean Journal of Ecology
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• 제10권3호
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• pp.109-118
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• 1987

The production and decomposition rates of litters were studied in three mountains, Mt. Halla, Mt. So-back and Mt. Tae-back. The amounts of N, P, K, Ca and Na in the soils were measured and the relationships between the mineral nutrients and decay rates were reserched, The annual litter production was the most as $1, 077.3g/m^2$ and the least as $248.0g/m^2$ in a stand of Quercus acutissima at 820m of the Mt. So-back and at 1, 350m of the Mt. So-back among the study sites, respectively. The decay rates of litter was the highest as k=0.448 and the lowest as k=0.082 in a stand of Q. acutissima at 820m of the Mt. So-back and at 1, 450m of the Mt. Tae-back at 1, 450m of the Mt. Tae-back among the study sites. The higher altitude was, the slower the decay rates were. The annual contents of mineral nutrient and their amounts inputted into the forest soil were proportional to the decay rate of organic metters.

• Fisheries and Aquatic Sciences
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• 제13권2호
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• pp.112-117
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• 2010

Altered mRNA expression of two metallothionein isoforms (MT-IA and MT-IB) in response to acute heavy metal exposure was examined in mud loach, Misgurnus mizolepis, fry using a real-time RTPCR assay. Sublethal exposure (1 or 5 ${\mu}M$) to Cd, Cr, Fe, Mn, Ni, and Zn resulted in highly variable transcriptional responses of the two MT isoforms to the heavy metal ions, including upregulation, a steady state, and downregulation. Overall, the most potent inducer of both MT isoforms was Cd (up to 6-fold). Another exposure experiment using a series of doses of Cu revealed that the stimulation patterns of the two MT isoforms differed: MT-IA transcription was soon saturated at higher concentrations (about 2-fold at 1-4 ${\mu}M$ of Cu), whereas the activation of MT-IB was more dependent on the treatment dose (increased up to 5-fold at 3 ${\mu}M$). The isoform-specific allotment of constitutive and inducible functions was not as clear in fry as in adult tissues. Coordinated interaction between the MT-IA and MT-IB isoforms was hypothesized based on the finding that MT-IA represented a primary action under 'less stressful' or 'sublethal' conditions, whereas the activation of MT-IB became important under 'more stressful' or 'lethal' circumstances in this species.

• Animal cells and systems
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• 제5권3호
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• pp.263-266
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• 2001

Metallothionein (MT) is induced in the regenerating rat liver. We have investigated expression of MT gene by RT PCR as well as specific localization of MT by immunocytochemistry in regenerating rat liver after partial hepatectomy (PH). MT mRNA level started to increase from 1 h and reached the peak at 8 h after PH. The level decreased gradually by 24 h, and became similar to that of control group. In the immunocytochemical study, in all groups treated with primary antibody, immunogold particles indicating the presence of MT were evenly distributed throughout both cytoplasm and nucleus of the rat hepatocytes. Within the nucleus, the gold particles appeared to be intensely localized in the areas of euchromatin and nucleolus. Within the cytoplasm, gold particles did not seem to adhere to mitochondria or Iysosomes, but were freely distributed. However, rough endoplasmic reticulum was the obvious compartment on which the gold particles were localized. Time course of MT immunoreactivity revealed that distribution of gold particles in hepatocytes increased gradually by 24 h, and decreased at 48 h after PH. Briefly, PH resulted in the sharpest increase in the expression of MT mRNA at 8 h and in the immunoreactivity of MT at 24 h, respectively. It is suggested that the increase of MT mRNA expression, the intensity of immunoreactivity and the specific localization of MT may be associated with the compensatory cell proliferation followed by PH.