• KSBB Journal
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• v.10 no.1
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• pp.23-29
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• 1995

Calli were induced from leaf base region of germinated rice(Oryza sativa L. cv. Nakdong) with high frequency of up to 65% on LS medium supplemented with $2.5mg/{\ell}2$, 4-D in the dark at $27^{\circ}C$. Embryogenic calli of pale yellow, globular type were selected and used for the initiation of cell suspension cultures in AA2 liquid medium with $2mg/\ell$ 2,4-D, 0.2mg/$\ell$ kinetin arid $0.1mg/\ell$ GA3. Protoplasts were isolated from the embryogenic cell suspensions after 4 months of culture and then were electroporated with 400V/cm for 1 msec. Electroporated protoplasts divided with plating efficiency of 1.1% on PCM liquid medium supplemented with $2.5mg/\ell$ 2, 4-D, $0.1mg/\ell$ kinetin and 10mM proline. The protoplasts-derived microcalli were cultured on $0.2{\mu}m$ membrane fitter placed onto LS2.5 solid medium containing fine suspension cells as a feeder cells, for 2 weeks in the dark at $27^{\circ}C$. After an additional 2 weeks of culture under fluorescent light of $30{\pm}/3{\mu}E$.m^{-2}S^{-1}, yellow calli of 2mm diameter were transferred to regeneration medium. Shoots were produced from the green spot of protoplasts-derived calli and plants were regenerated form protoplast-derived green calli with frequencies of 11∼33%.

• Proceedings of the KSRS Conference
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• v.2
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• pp.1011-1014
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• 2006

Coarse resolution (9 - 50 km pixels) Sea Surface Temperature satellite data are frequently considered adequate for open ocean research. However, coastal regions, including coral reef, estuarine and mesoscale upwelling regions require high-resolution (1-km pixel) SST data. The AVHRR SST data often suffer from navigation errors of several kilometres and still require manual navigation adjustments. The second serious problem is faulty and ineffective cloud-detection algorithms used operationally; many of these are based on radiance thresholds and moving window tests. With these methods, increasing sensitivity leads to masking of valid pixels. These errors lead to significant cold pixel biases and hamper image compositing, anomaly detection, and time-series analysis. Here, after manual navigation of over 40,000 AVHRR images, we implemented a new cloud filter that differs from other published methods. The filter first compares a pixel value with a climatological value built from the historical database, and then tests it against a time-based median value derived for that pixel from all satellite passes collected within ${\pm}3$ days. If the difference is larger than a predefined threshold, the pixel is flagged as cloud. We tested the method and compared to in situ SST from several shallow water buoys in the Florida Keys. Cloud statistics from all satellite sensors (AVHRR, MODIS) shows that a climatology filter with a $4^{\circ}C$ threshold and a median filter threshold of $2^{\circ}C$ are effective and accurate to filter clouds without masking good data. RMS difference between concurrent in situ and satellite SST data for the shallow waters (< 10 m bottom depth) is < $1^{\circ}C$, with only a small bias. The filter has been applied to the entire series of high-resolution SST data since1993 (including MODIS SST data since 2003), and a climatology is constructed to serve as the baseline to detect anomaly events.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2003.05a
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• pp.149-156
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• 2003

This paper presents an underwater hybrid navigation system for a semi-autonomous underwater vehicle (SAUV). The navigation system consists of an inertial measurement unit (IMU), an ultra-short baseline (USBL) acoustic navigation sensor and a doppler velocity log (DVL) accompanying a magnetic compass. The errors of inertial measurement units increase with time due to the bias errors of gyros and accelerometers. A navigational system model is derived to include the error model of the USBL acoustic navigation sensor and the scale effect and bias errors of the DVL, of which the state equation composed of the navigation states and sensor parameters is 25 in the order. The conventional extended Kalman filter was used to propagate the error covariance, update the measurement errors and correct the state equation when the measurements are available. Simulation was performed with the 6-d.o.f. equations of motion of SAUV in a lawn-mowing survey mode. The hybrid underwater navigation system shows good tracking performance by updating the error covariance and correcting the system's states with the measurement errors from a DVL, a magnetic compass and a depth senor. The error of the estimated position still slowly drifts in horizontal plane about 3.5m for 500 seconds, which could be eliminated with the help of additional USBL information.

• ETRI Journal
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• v.41 no.2
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• pp.184-196
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• 2019

Wavelet packet modulation (WPM) is a multicarrier modulation (MCM) technique that has emerged as a potential alternative to the widely used orthogonal frequency-division multiplexing (OFDM) method. Because WPM has overlapped symbols, equalization cannot rely on the use of the cyclic prefix (CP), which is used in OFDM. This study applies linear minimum mean-square error (MMSE) equalization in the time domain instead of in the frequency domain to achieve low computational complexity. With a modest equalizer filter length, the imperfection of MMSE equalization results in subcarrier attenuation and noise amplification, which are considered in the development of a bit-loading algorithm. Analytical expressions for the bit error rate (BER) performance are derived and validated using simulation results. A performance evaluation is carried out in different test scenarios as per Recommendation ITU-R M.1225. Numerical results show that WPM with equalization-aware bit loading outperforms OFDM with bit loading. Because previous comparisons between WPM and OFDM did not include bit loading, the results obtained provide additional evidence of the benefits of WPM over OFDM.

• Journal of Plant Biotechnology
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• v.32 no.1
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• pp.23-29
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• 2005

Shoots of balloon flower (Platycodon grandiflorum A. DC.) derived from in vitro germinated seeds were cultured on MS medium containing $0.1\;\cal{mg/L}$ NAA under various photosynthetic photon flux (PPF) 33, 66, and $99\;{\mu}mol\;m^{-2}s^{-1}$ with or without membrane filter. Number of air exchanges per hour (NAEH) of the culture vessel with membrane filter on the lid was $4.9 h^{-1}$ and that without membrane filter was $0.1 h^{-1}$ Plantlets grown in $4.9 h^{-1}$ NAEH showed greater growth than in $0.1 h^{-1}$ NAEH. According to increase of PPF, plantlets growth decreased in $0.1 h^{-1}$ NAEH while it increased in $4.9 h^{-1}$ NAEH. At the same PPF, fresh weight and sugar content in plantlets in $4.9 h^{-1}$ NAEH were above 1.9, 2.0 times higher than those in $0.1 h^{-1}$ NAEH, respectively. Also they were enhanced in $4.9 h^{-1}$ NAEH by increase of PPF whereas no significance in $0.1 h^{-1}$ NAEH. The percentage of water content of plantlets in $4.9 h^{-1}$ NAEH was $4.2\~5.5\%$ lower than those in $0.1 h^{-1}$ and no difference in PPF. The content of total chlorophyll in plantlets in $4.9 h^{-1}$ NAEH was higher $0.27\~0.79\;\cal{mg/g}$ F.W. than that in $0.1 h^{-1}$ NAEH. By increase of PPF, it was decreased in $0.1 h^{-1}$ NAEH while had no significant difference in $4.9 h^{-1}$ NAEH. Guard and subsidiary cells of leaves in $4.9 h^{-1}$ NAEH were more developed than in $0.1 h^{-1}$ NAEH. Especially, in $99\;{\mu}mol\;m^{-2}s^{-1}$ leaves in $0.1 h^{-1}$ NAEH had undeveloped subsidiary cells and wide open stomata whereas those in $4.9 h^{-1}$ NAEH had well-developed subsidiary cells.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.42 no.12
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• pp.45-52
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• 2005

A systolic array formed by interconnecting a set of identical data-processing cells in a uniform manner is a combination of an algorithm and a circuit that implements it, and is closely related conceptually to arithmetic pipeline. High-performance computation on a large array of cells has been an important feature of systolic array. To achieve even higher degree of concurrency, it is desirable to make cells of systolic array themselves systolic array as well. The structure of systolic array with its cells consisting of another systolic array is to be called super-systolic array. This paper proposes a scalable bit-level super-systolic amy which can be adopted in the VLSI design including regular interconnection and functional primitives that are typical for a systolic architecture. This architecture is focused on highly regular computational structures that avoids the need for a large number of global interconnection required in general VLSI implementation. A bit-level super-systolic FIR filter is selected as an example of bit-level super-systolic array. The derived bit-level super-systolic FIR filter has been modeled and simulated in RT level using VHDL, then synthesized using Synopsys Design Compiler based on Hynix $0.35{\mu}m$ cell library. Compared conventional word-level systolic array, the newly proposed bit-level super-systolic arrays are efficient when it comes to area and throughput.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.78-78
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• 2003

Embryonic stem (ES) cells, derived from preimplantation embryo, are able to differentiate into various types of cells consisting the whole body, or pluripotency. In contrast, terminally differentiated cells do not usually alter their nature but frequently die or transform if they are exposed to inappropriate external stimulations. In addition to the plasticity, ES cells are expected to be different from terminally differentiated cells in very many ways, such as patterns of gene expressions, ability and response of the cells in confronting environmental stimulations, metabolism, and growth rate. As a model system to differentiate these two types of cells, human ES cells (MB03) and terminally differentiated cells (HeLa), we examined the ability of these two types of cells in confronting a severe oxidative insult, that is $H_2O$$_2$. Approximately 1$\times$10$^4$ cells were plated in 96 well plate and serum starved for overnight. The conditioned cells were exposed to a various concentration of $H_2O$$_2$ fur 24 hrs and loaded with neutral red (50$\mu\textrm{g}$/ml) for 4 hrs, washed with PBS for 2 min three times, and entrapped dye was dissolved out using acetic ethanol. Cytotoxicity was determined by reading the amount of dye in the medium using microplate reader. equipped with 575 nm filter. Relative amount of the dye entrapped within MB03 or HeLa were not significantly different when cells were exposed up to 0.4 mM $H_2O$$_2$. However, this sharply decreased down to 0.12% in HeLa cells when the cells were exposed to 0.8 mM $H_2O$$_2$, while it was approximately 54% in MB03 suggesting that this concentration of $H_2O$$_2$ is the defensive threshold for HeLa cells. The resistance to oxidative stimulation reversed, however, when cells were co-treated with BSO (L-buthionine- 〔S, R〕-sulfoximine) which chelates intracellular GSH. This result suggests that cellular GSH is the major defensive mechanism of human ES cells. Induction of enzymes involved in GSH metabolism and type of cell death is currently being studied.

• The Journal of Engineering Geology
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• v.30 no.4
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• pp.603-615
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• 2020

The design factors of artificial recharge are determined by considering the hydrogeological characteristics of the aquifer. The optimal design factors for artificial recharge were derived after performing the injection tests step by step for each injection type (vertical well, ditch and mixed type), which were built in the test site of the study area. It was analyzed that the difference in the injection effect according to the diameter of the injection well was not large, and the 100 mm well was evaluated as appropriate in consideration of the availability and economy of land use. Since the injection effect was well maintained even in the upper rock, the depth of the injection well was proposed for the alluvial layer and the upper rock layer. On the other hand, in four cases of filter media in the ditch, it was analyzed that the penetration efficiency and the hydraulic interference effect indicated excellent injection performance when a filter medium of 10 to 30 mm diameter was filled in the ditch. In addition, the proper spacing of the injection wells was analyzed as 9~12 m considering the interference efficiency. The interference efficiency attenuation coefficient per 1 m of hole spacing was calculated to be 1.75% in this area. In the future study, the artificial recharge design factors obtained in this stage are applied and verified on site construction and operation. Also it is expected to contribute to securing water in areas where there is always a lack of water.

• Korean Journal of Ecology and Environment
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• v.51 no.3
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• pp.245-252
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• 2018

Stable isotope tracers were first applied to evaluate the Microcystis cell assimilation efficiency of bivalves, since the past identification method has been limited to tracking the changes of each chl-a, clearity, and nutrient. The filter-feeders (Sinanodonta woodiana and Unio douglasiae) were assessed under the condition of cyanobacteria (Microcystis aeruginosa) blooms through an in mesocosm experiment using $^{13}C$ and $^{15}N$ dual isotope tracers. chl-a concentration in the treatment mesocosm was dramatically decreased after the beginning of the second day, ranging from 116 to $66{\mu}g\;L^{-1}$. In addition, the incorporated $^{13}C$ and $^{15}N$ atom % in the S. woodiana bivalve showed higher values than U. douglasiae bivalves. The results demonstrate that U. douglasiae has less capacity to assimilate toxic cyanobacteria derived from diet. Our results therefore also indicate that S. woodiana can eliminate the toxin more rapidly than U. douglasiae, having a larger detoxification capacity.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.26 no.3
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• pp.443-448
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• 2022

This paper presents a subband affine projection algorithm with variable step size to improve convergence performance in adaptive filtering applications with long adaptive filters and highly correlated input signals. The proposed algorithm can obtain fast convergence speed and small steady-state error by using different step sizes for each adaptive sub-filter in the subband structure to which polyphase decomposition and noble identity are applied. The step size derived to minimize the mean square error of the adaptive filter at each update time shows better convergence performance than the existing algorithm using a variable step size. In order to confirm the convergence performance of the proposed algorithm, which is superior to the existing algorithm, computer simulations are performed for mean square deviation(MSD) for AR(1) and AR(2) colored input signals considering the system identification model.