• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.4
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• pp.387-394
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• 1983

The factor, which was capable of accelerating the yeast cell wall lysis of the zymolyase(${\beta}-1$, 3-glucanase), was purified to a homogeneous state from the protease fraction of the crude zymolyase by Sephadex G-75 gel filtration and preparative polyacrylamide gel disc electrophoresis. The molecular weight of the purified factor was estimated to be 40,500 by SDS-polylacrylamide gel disc electrophoresis and it's iso-electric point was pH 9.6. The factor was found to be a basic protease consisted of single polypeptide chain with 395 amino acid residues and it showed the $E_{280,cm}^{1%}$ of 11.9 and the molecular extinction coefficient of $4.83{\times}10^4$, respectively.

• Journal of Microbiology
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• v.35 no.4
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• pp.354-359
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• 1997

Methods for the extraction of DNA from water sample were approximated. Four different procedures of DNA extraction were carried out with pellets obtained from centrifugation of 4 liter water samples. The recovery efficiency and purity of DNA extracted by each method from different sources were compared. DNA yield varied with extraction methods, Method I, which involves enzymatic and freeze-thaw lysis steps and phenol and phenol-chloroform purification of extracted nucleic acid, showed a significantly higher yield and purity than the other methods. The use of glass beads in the DNA extraction methods improved the purity of DNA suitable for PCR. Bovine serum albumin in the PCR reaction mixture was useful in reducing inhibitory effects of contaminants. The efficiency of an extraction method was determined by the detection of the aer of Aeromonas hydrophila with PCR. The lower limit of detection of A. hydrophila from seeded tap water was 2 CFU/ml in PCR when method I was used for DNA preparation.

• Preventive Nutrition and Food Science
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• v.1 no.2
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• pp.269-277
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• 1996

Bacteriocins are proteins produced by a heterogeneous group of bacteria that have a bactericidal effect on closely related organisms. Recently, bacteriocins from lactic acid bacteria and other food-related organisms have been the subject of much research because of their potential as food biopreservatives. Various modifications of agar plate diffusion assays are the most widely used methods even though the limitations of such assays are generally recognized. The ability to obtain a concentrated crude preparation on bacteriocin by optimizing production parameters greatly simplifies recovery of bacteriocin on subsequent purification steps. Some studies performed to optimize bacteriocins have been purified to homogeneity, and the amino acid sequences of many of these purified bacteriocins have been determined. Obtaining characterization data on purified bacteriocin will minimize the risk of overlapping of research and confusion on identification of these compounds. Several me-chanisms leading to cell death have been hypothesized. These include depletion of the proton motive force(PMF) across the cell membrane: RNase and/or DNase activity within the sensitive cell; and pore formation and lysis of sensitive cells at the cell membrane.

• Journal of Acupuncture Research
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• v.25 no.3
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• pp.87-94
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• 2008

Objectives : This study was to compare the effect of moxibustion therapy on peripheral facial para- lysis according to location of acupoint. Methods : We investigated 41 cases of inpatients with Peripheral Facial Paralysis, and divided them into two groups, One group was treated by moxibustion on local point(ML), and the other was treated by moxibustion on distant point(MD). We evaluated the effect of moxibustion in each group by using Gross Grading System of House-Brackmann(HB score) and Yanagihara's Unweighed Grading System(Y score). Results : 1. In ML and MD, compared with baseline, at final, HB score was significantly decreased and Y score was significantly increased. 2. At final, ML showed significant decrease on HB score and significant increase on Y score compared with MD. Conclusions : These results suggested that ML should be more useful for improving symptoms related with peripheral facial paralysis than MD.

• Journal of the Korean Society for information Management
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• v.9 no.2
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• pp.118-153
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• 1992

This study att.empts to conduct a system an;~lysis and dcsign for circt~lation control in a univcr~ity library. In this proccss, structured sysltcm analys15 tcrhn~clucs Iikr data flolv diagrnms. data dictiomiry, and entity - relationship analysis, arc. vmploycd to construct both concqitual and p h p c a l data models of current and new circulation systems. Thc whole design aims at an al~tomntctf c~r.culation system on thc [nlcro-compurcr --basis in a mid dle - sized library.

• Korean Journal of Veterinary Research
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• v.30 no.1
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• pp.29-33
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• 1990

The study was attempted to scrutinize the normal osmotic fragility of erythrocyte in the domestic ruminants, making a comparison with that of human and canine. The animals used in the experiment were apparently healthy adult Holstein cattle, Korean native cattle, sheep (Corriedale) and Korean native goats. Osmotic fragility of erythrocytes was the highest in the Korean native goats, and the next were sheep and Korean native cattle, and Holstein cattle in order. In other words, erythrocytic resistance to osmotic lysis was the strongest in Holstein cattle and the most weak in Korean native goats among the domestic ruminants. In general, resistance of erythrocytes was stronger in human and canine than in the domestic ruminants.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.582-585
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• 2001

GL, a glycolipid type produced from yeast, inhibited the growth of bacteria and fungi. Algicidal effect by GL against dinoflagellate was motility stop and lysis. When over 10 ${\mu}g/ml$ showed strong algicidal effect. GL attached to the algal body directly.

• Journal of Plant Biology
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• v.36 no.4
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• pp.327-335
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• 1993

The active sites, intracellular transport, function of cellulase in association with the disintegration of the storage materials of the endosperm cells during seed maturation and after-ripening of Panax ginseng C.A. Meyer seeds were studied by electron microscopy. Cytochemical activities of the cellulase occurred in protein bodies and vesicles of endosperm cells in seed with red seed coat. In after-ripening seed, the activities were strongly found in the cell wall of endosperm near the umbiliform layer and on neighbouring vesicles, so it is assumed that these cells begin to be decomposed. Cellulase activities were initiated before the decomposition of storage materials. But, no activity was observed in the umbiliform layer, so it is suggested that cellulase lose its activity after the completion of lysis process.

• Biomolecules & Therapeutics
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• v.1 no.2
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• pp.196-203
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• 1993

We compared in vitro antibacterial activity of DWC-751, a new parenteral cephalosporin antibiotic, with those of cefpirome (CPR), cefotaxime (CTX) and ceftazidime (CAZ). DWC-751 showed a broad antimicrobial spectrum against Gram-positive and negative bacteria. The antibacterial activity of DWC-751 against Stapylococcus aureus was equal to that of CPR and superior to those of CTX and CAZ. The activity of it against Excherichia coli was more potent than those of CPR, CTX and CAZ. Against Pseudomonas aeruginosa, DWC-751 was slightly inferior to that of CAZ and superior to those of CPR and CTX. The antibacterial activity of DWC-751 was superior to those of CPR, CTX and CAZ against clinical isolates and ofloxacin resistant strains. DWC-751 showed bactericidal action against Escherichia coli at concentrations close to the MIC and induced the formation of filament and burge and lysis of Escherichia coli in a microscopic examination.

• Preventive Nutrition and Food Science
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• v.17 no.1
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• pp.78-82
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• 2012

Antithrombotic and fibrinolytic activity of natto was evaluated on platelet aggregation in vitro and in vivo. Natto showed inhibitory effects on platelet aggregation induced by adenosine 5’diphosphate (ADP) and collagen. Orally administered natto also showed fibrinolytic activity in hypercholesterolemia rats. Normal levels of natto, when administered for four weeks, shortened euglobulin clot lysis time (ECLT) and prolonged partial thromboplastin time (PATT) significantly compared to non-treated group. In addition, the natto treatment decreased total cholesterol in serum. These results showed that intake of normal levels of natto can elicit antithrombotic and fibrinolytic effects, suggesting its consumption may improve blood circulation.