• 대한수의학회지
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• 제36권3호
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• pp.647-655
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• 1996

To establish the effective ways to prevent bovine mastitis, the study has been performed to investigate the attributable factors causing down-regulation of immune responses in mammary gland of non-lactating cows. Lymphocytes from peripheral blood and mammary gland secretions(MGS) were obtained from normal healthy cows and mastitic cows, respectively. Lymphoblastogenesis were investigated carefully by adding different concentrations of supernatants collected from pure-cultures of neutrophils seperated from peripheral blood and MGS, respectively. The results obtained are as follows ; 1. Lymphoblastogenesis activity stimulated by Con A, PWM and PHA were significantly reduced in MGS from mastitic cows. 2. Supernatants collected from pure-culture of neutrophils separated both from peripheral blood and MGS showed inhibitory effect on mitogenic lymphoblastogenesis. 3. Supernatants from mammary gland neutrophils have shown 7 times more inhibitory activity than those from peripheral blood and this inhibitory effect was increased in proportion to increasing concentrations of supernatants when those were added to lymphoblast cells in culture.

• Asian-Australasian Journal of Animal Sciences
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• 제20권9호
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• pp.1438-1443
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• 2007

The experiment was conducted to evaluate the effects of Ligustrum lucidum (LL) and Schisandra chinensis (SC) on the growth, antioxidative metabolism and immunity of laying strain male chicks. The results showed that diets supplemented with 1% of either LL or SC had no effects on the growth performance of chicks compared with the control. Furthermore, both LL and SC significantly reduced malondialdehyde (MDA) concentration of serum and heart of chicks (p<0.05). In addition, superoxide dismutase (SOD) activity of serum of the birds was significantly elevated by supplementation with SC (p<0.05). Glutathione reductase (GR) activity of heart and serum of the birds was significantly elevated by supplementation with LL or SC (p<0.05). LL supplementation significantly elevated antibody values against Newcastle Disease virus (NDV)(p<0.05) and lymphoblastogenesis (p<0.05) of the birds. The results suggest that diets supplemented with 1% of either LL or SC may improve immune function and antioxidant status of chicks.

• 생약학회지
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• 제25권4호통권99호
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• pp.348-355
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• 1994

An antitumor component, F-D-P, was purified from the hot water extract of the carpophores of Elfvingia applanata by precipitation with ethanol, dialysis, and passage through a column of DEAE-cellulose ion exchange. F-D-P inhibited the growth of Sarcoma 180 in mice showing the tumor inhibition ratio of 88.3% in doses of 20 mg/kg for ten days. Chemical analysis of F-D-P showed that it was composed of polysaccharide(65.3%) and protein(6.5%0, and that the monosaccharides consisting of the polysaccharide was glucose(89.1%) and mannose(10.9%). The immunomodulatory activities of F-D-P were explored by determining its effect on the proliferation of the whole and subpopulations of lymphocytes, and on the generation of natural killer(NK) cell activity in vitro. F-D-P was mitogenic to total lymphocytes and B cells, but not to purified T cells, even in the presence of accessory cells. F-D-P did not increase NK cell activity when added to cultures of resting lymphocytes. From these results, it is clear that F-D-P modulates primarily the humoral immune responeses.

• 한국자원식물학회지
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• 제23권2호
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• pp.157-164
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• 2010

본 연구에서는 해당화의 과육(RRF)과 종자(RRS) 부위의 추출물이 골다공증 및 골질환에 미치는 영향을 연구하기 위하여 각각 조골세포의 세포 증식능과 LPS로 유도된 염증상태에서 전구-파골세포가 염증유발 물질로 분비하는 NO와 TNF-$\alpha$ 분비 억제 조절능을 측정하였다. 또한, 과육(RRF)과 종자(RRS)가 골다공증 및 골질환이 빈번하게 발생하는 노화된 상태에서도 적용할 수 있는지 확인하기 위하여 정상생쥐와 노화생쥐로부터 분리한 비장세포에서 B 림프구와 T 림프구의 세포 증식능을 측정하였다. 연구 결과에서 과육(RRF)과 종자(RRS)는 그 효능과 농도에서 차이를 나타내었으나, 모두에서 조골세포의 세포 증식효과와 LPS에 의한 NO 분비 억제효과를 보였으며, TNF-$\alpha$ 조절에는 영향을 나타내지 않았다. 그러나 종자(RRS)는 $500\;{\mu}g/m{\ell}$에서 pre-osteoclastic cell에 세포 독성을 보였으며, LPS 에 의해 유도 증가된 TNF-$\alpha$의 분비도 오히려 증가시켰다. 따라서 과육(RRF)이 종자(RRS)보다 안전하고 효능성 있게 사용할 수 있음을 나타내었다. 정상 및 노화생쥐의 비장 세포에 대한 림프구 증식효과에서도 과육(RRF)과 종자(RRS)는 모두 비장세포 증식을 나타내었고, ConA 처리에 의한 T세포 증식에도 효과를 나타내었다. 그러나 LPS에 의한 B림프구의 세포증식효과에서 과육(RRF)의 경우에는 효과를 나타내지 않았나, 종자(RRS) $1000\;{\mu}g/m{\ell}$에서는 LPS 처리에 의한 B림프구의 증식을 오히려 감소시킴으로써 B 림프구의 면역을 억제할 수 있음을 나타내었다. 과육(RRF)과 종자(RRS)는 정상 및 노화생쥐에서 같은 양상을 보임으로써 노화된 상태에서 특별히 다른 효과를 보이지는 않았으나, 종자(RRS)가 LPS에 의한 B세포의 증식에 오히려 감소효과를 나타낸 점을 고려할 때, 종자(RRS)에 대해서는 처리 농도와 효능에 대한 연구가 더욱 이루어져야 할것으로 생각되며, 과육(RRF)이 노화 및 정상상태에서의 골다공증 및 골질환에 좀 더 안전하고 효능성 있게 사용될 수 있을 것으로 사료된다.