• Journal of Gastric Cancer
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• 제13권3호
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• pp.192-195
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• 2013

Gastrointestinal stromal tumor is a rare tumor which arises from the whole gastrointestinal tracts and most of it is detected in the stomach. It is uncommon with small intestine originated gastrointestinal stromal tumor and more uncommon with lymphatic metastasis. We experienced an unusual case of the small bowel gastrointestinal stromal tumor during experimental autopsy. Two primary tumors with central necrosis were detected in the ileum. The sizes of each tumor were $6.1{\times}3.4{\times}4.0$ cm and $3.7{\times}4.2{\times}3.2$ cm. There was extensive lymphatic metastasis on the greater omentum and mesenteric, iliac lymph nodes were also involved. With histologic findings, the eosinophilic spindle cells were densely distributed. Immunohistochemical findings were CD117 (-), CD34 (+), desmin (-), and S-100 protein (-). Therefore, we diagnosed the tumors as small bowel gastrointestinal stromal tumors with broad lymph node mestasis.

• 대한수의학회지
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• 제34권2호
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• pp.229-235
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• 1994

The hemal nodes of the Korean native cattle were studied by gross anatomy and light microscopy. Hemal nodes were spherical or ovoid in shape, and red or black in colors. A hemal node consisted of a thick capsule and a hilum, and had extensive subcapsular and deep sinuses distended by a great number of erythrocytes. Although a few lymphatic nodules and tissues were seen in the parenchyma, no typical cortex and medulla was defined. Blood vessels occurred, but lymph vessel was not observed in nodes. The stroma of the hemal node was composed of reticular cells and fibers. The parenchyma consisted of many erythrocytes and lymphocytes, and a few macrophages and megakaryocytes. The capsule and trabecula was a collagenous connective tissue with smooth muscle cells. B-lymphocytes were principally located in the lymphatic nodules of the hemal node. T-lymphocytes were scattered in the diffuse lymphatic tissues of the hemal node.

• Journal of Magnetics
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• 제18권2호
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• pp.188-191
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• 2013

The magnetic properties for sanal's mobility inside of the lymphatic primo vascular system, the so-called Kyungrak (or meridian) system, are investigated under a low static magnetic field with the anatomy technology and optical microscope. One sanal with a size of 1 ${\mu}m$ under microscope selected and separated from the primo vessels of the primo vascular system are observed in rabbits' lymphatic vessels around abdominal aorta and placed in PBS solution with petridish. The moving displacement of sanal versus the measuring time of 20 Oe below a magnetic field of 80 Oe is stronger in dominanting dependence according to the x-direction than y-direction.

• 핵의학기술
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• 제28권1호
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• pp.13-17
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• 2024

Bone Mineral Densitometry (BMD) values is determined numerically with T or Z-score while the image is not considered in the interpretation. Abnormal finding was observed in the left femoral bone density image of a 64-year-old female patient who underwent a left kidney transplantation. This was due to the ethiodized oil used for embolization in the treatment of lymphatic leakage. Radiologic technologists should not only monitor changes of BMD values, but keep in mind to assess the changes in the image to provide accurate information to the patient.

• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1363-1367
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• 2014

This research was conducted to compare differences in colon cancer lymphatic vessel invasion (LVI) with D2-40 antibody labeling and regular HE staining, blood vessel invasion (BVI) with CD34 antibody labeling and HE staining and to assess the possibility of using D2-40-LVI/CD34-BVI in combination for predicting stage II colon cancer prognosis and guiding adjuvant chemotherapy.Anti-D2-40 and anti-CD34 antibodies were applied to tissue samples of 220 cases of stage II colon cancer to label lymphatic vessels and small blood vessels, respectively. LVI and BVI were assessed and multivariate COX regression analysis was performed for associations with colon cancer prognosis. Regular HE staining proved unable to differentiate lymphatic vessels from blood vessels, while D2-40 selectively labeled lymphatic endothelial cell cytosol and CD34 was widely expressed in large and small blood vessels of tumors as well as normal tissues. Compared to regular HE staining, D2-40-labeling for LVI and CD34-labeling for BVI significantly increased positive rate (22.3% vs 10.0% for LVI, and 19.1% vs 9.1% for BVI). Multivariate analysis indicated that TNM stage, pathology tissue type, post-surgery adjuvant chemotherapy, D2-40-LVI, and CD34-BVI were independent factors affecting whole group colon cancer prognosis, while HE staining-BVI, HE staining-LVI were not significantly related. When CD34-BVI/D2-40-LVI were used in combination for detection, the risk of death for patients with two or one positive results was 5.003 times that in the LVI(-)&BVI(-) group (95% CI 2.365 - 9.679). D2-40 antibody LVI labeling and CD34 antibody BVI labeling have higher specificity and accuracy than regular HE staining and can be used as molecular biological indicators for prognosis prediction and guidance of adjuvant chemotherapy for stage II colon cancer.

• 대한화장품학회지
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• 제50권2호
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• pp.119-129
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• 2024

인체 피부 림프계는 간질액을 배출하고 면역 시스템을 활성화하는 중요한 역할을 한다. 자외선과 자연적인 노화와 같은 환경 요인들은 종종 이러한 림프관의 구조적 변화를 일으키며, 이로 인해 피부 기능 장애를 발생시키기도 한다. 그러나 이러한 연구를 위한 동물 실험 대체 방안이 없기 때문에 여전히 연구를 진행하기엔 적합하지 않은 제한 사항들이 존재한다. 인체 피부 림프계를 더 잘 이해하고 림프관 형성에 관련된 분자 및 생리학적 변화를 조사하기 위해, 생체 모방 미세유체 플랫폼인 'skin-lymph-on-a-chip'을 제작하여, 새로운 체외 인체 피부 림프 모델을 개발하였다. 간단히 말해, 이 플랫폼은 체외에서 분화된 일차 정상 인간 표피 각질형성세포(NHEKs)와 피부 림프 내피세포(HDLECs)를 공동 배양하는 것을 의미한다. 약 500 시간 동안 자연 발효를 통해 확보하고 집약된 인삼 뿌리 추출물인 Lymphanax^TM의 림프관 형성 효과를 평가하기 위해 해당 시스템에 적용하였고, 분자 수준 요인들의 변화는 딥러닝 기반 알고리즘을 사용하여 분석하였다. 결론적으로, Lymphanax^TM는 skin-lymph-on-a-chip에서 건강한 림프관 형성을 촉진하였고, 그 성분들이 칩내에서 분화된 NHEKs를 거의 침투하지 않는 결과를 통해, HDELCs에 간접적으로 영향을 미쳤음을 확인하였다. 전반적으로, 이 연구는 기존과 차별화된 체외 인체 피부 림프모델 시스템의 확보와 더불어 이를 통한 Lymphanax^TM의 림프 활성화 효과에 대한 새로운 관점을 제공한다.

• Journal of Chest Surgery
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• 제28권2호
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• pp.209-212
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• 1995

Cystic hygroma is a cystic tumor developed by lymphatic stasis due to congenital blockage of regional lymphatic drainage. It ususally occurs at neck and axilla. However, in some cases of cervical cystic hygroma, cervical portions of the cysts may extend into the mediastinum. We experienced a case of huge cervico-mediastinal cystic tumor in 11 month old girl presenting a large fluctuating neck mass and severe respiratory distress. Surgical resection was done through combined approach of cervical incision and median sternotomy. Pathological diagnosis was confirmed to cervico-mediastinal cystic hygroma.

• 한국영양학회:학술대회논문집
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• 한국영양학회 1997년도 추계학술대회 초록
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• pp.37-37
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• 1997

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2823-2828
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• 2012

Objective: To explore a new model of in-situ xenograft lymphangiogenesis of human colonic adenocarcinomas in nude mice. Method: On the basis of establishing subcutaneous xenograft lymphangiogenesis model of human colonic adenocarcinoms, in-situ xenografts were established through the in situ growth of the HT-29 human colonic adenocarcinoma cell line in nude mice. The numbers of lymphangiogenic microvessels, the expression of lymphatic endothelial cell markers lymphatic vessel endothelial hyaloronic acid receptor-1 (LYVE-1), D2-40 and the lymphatic endothelial growth factors vascular endothelial growth factor-C (VEGF-C), -D (VEGF-D) and receptor-3 (VEGFR-3) were compared by immunohistochemical staining, Western bolt and quantitative RT-PCR in xenograft in-situ models. Results: Some microlymphatics with thin walls, large and irregular or collapsed cavities and increased LMVD, with strong positive of LYVE-1, D2-40 in immunohistochemistry, were observed, identical with the morphological characteristics of lymphatic vessels and capillaries. Expression of LYVE-1 and D2-40 proteins and mRNAs were significantly higher in xenograpfts in-situ than in the negative control group(both P<0.01). Moreover, the expression of VEGF-C, VEGF-D and VEGFR-3 proteins and mRNAs were significantly higher in xenografts in-situ (both P<0.01), in conformity with the signal regulation of the VEGF-C,-D/VEGFR-3 axis of tumor lymphangiogenesis. Conclusions: In-situ xenografts of a human colonic adenocarcinoma cell line demonstrate tumor lymphangiogenesis. This novel in-situ animal model should be useful for further studying mechanisms of lymph node metastasis, drug intervention and anti-metastasis therapy in colorectal cancer.

• 대한수의학회지
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• 제37권3호
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• pp.463-469
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• 1997

This study was performed to investigate the gross anatomical features and microscopical structures of the hemal nodes and the hemolymph nodes in the water deer (Hydropotes inermis) found in Kangwon-do, Korea. The hemal nodes and hemolymph nodes were observed mainly in the periphery of the thoracic and abdominal aortae of the animals. The size of hemal nodes was generally smaller than that of the hemolymph nodes, and the shape of the both organs was spherical or ovoid. The color of the hemal nodes was red or black while that of the hemolymph nodes was gray with red bands. The hemal nodes were surrounded by a thin connective tissue capsule and there were extensive subcapsular and deep sinuses distended by a great number of erythrocytes. Although a few number of lymphatic nodules and small areas of diffuse lymphatic tissues were observed in the parenchyma, no typical cortex and medulla were defined in the hemal node. Small numbers of blood vessels were found at the connective tissue capsule but lymph vessel was not observed microscopically in this organ. The hemolymph nodes were covered by a relatively thick connective tissue capsule and there was a hilus in each node. The parenchyma was divided into cortex and medulla. The cortex was composed of a few numbers of lymphatic nodules and some diffuse lymphatic tissues. The medulla comprised medullary sinus and cords. Afferent and efferent lymph vessels were observed at the periphery of the capsule and the hilus, respectively. The subcapsular and medullary sinuses were not extensive but filled with small numbers of erythrocytes. The stroma of hemal node and hemolymph node was composed of reticular cells and fibers, and the capsule and trabecula consisted of collagenous fibers with smooth muscle fibers.