• Journal of Applied Biological Chemistry
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• 제65권4호
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• pp.269-275
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• 2022

자작나무(Betula platyphylla) 수피 추출물에는 생리활성을 보일 수 있는 다수의 terpenoids 물질이 포함되어 있고 염증반응을 완화시키는 치료제로 사용되어 왔다. 본 연구는 탈모예방 효과를 보일 수 있는 천연 물질을 확보하기 위해 자작나무를 여러 용매에서 추출 및 분리 정제하여 몇몇 분획을 획득하였다. 이를 사용하여 사람 모낭 모유두세포(HDP)에 대한 세포 독성 및 성장촉진 효과 그리고 β-catenin, VEGF, IGF1, Cyclin D의 발현변화와 5-α-reductase activity에 미치는 영향을 평가하였다. Ethyl acetate를 이용하여 분리한 4개의 분획 중 H3-2 분획은 알려진 유효물질인 ETB, LPO와 동등수준의 모낭 모유두세포(HDP)에 대한 성장 촉진 효과를 보였고 성장에 관여하는 주요인자들의 발현을 높이는 효과를 보였다. 또한, 5-α-reductase activity에 ETB, LPO가 큰 영향을 보이지 못한 반면 최종분획물인 H3은 5-α-reductase activity 감소활성이 있음을 확인할 수 있었다.

• Natural Product Sciences
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• 제22권4호
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• pp.225-230
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• 2016

Phytochemical investigation from the stem bark of Beilschmiedia pulverulenta resulted in the isolation of five lignans, (+)-yangambin (1), (+)-sesartemin (2), (+)-excelsin (3), (+)-sesamin (4), and (+)-syringaresinol (5), together with lupeol (6), lupenone (7), ${\beta}-sitosterol$ (8), and ${\beta}-sitostenone$ (9). Their structures were established by the analysis of their spectroscopic (1D and 2D NMR) and spectrometric (MS) data, as well as by comparison with those reported in the literature. The isolated lignans were tested for their anticholinesterase (AChE: acetylcholine esterase and BChE: butyryl cholineesterase) and anti-inflammatory (COX-2: cyclooxygenase-2 and LOX: lipoxygenase) activities. All the isolated lignans (1 - 5) exhibited significant inhibition activities in AChE/BChE and COX-2/LOX assays with $IC_{50}$ values ranging from $168.8-504.2{\mu}M$ and $21.0-59.4{\mu}M$, respectively.

• Applied Biological Chemistry
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• 제21권1호
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• pp.51-57
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• 1978

3종(種)의 가지과(科) 식물중(植物中)의 sterol조성(組成)을 TLC, GLC 및 GLC-MS에 의(依)해서 분석(分析)하여 4-desmethylsterol fraction에서 7종(種), 4-monomethylsterol fraction에서 9종(種), 그리고 4.4-dimethylsterol fraction에서는 8종(種) 모두 24종(種) sterol을 동정(同定)하였고, 이들 sterol들이 식물체내(植物體內)에서 어떤 생합성경로(生合成經路)를 거치는가를 검토(檢討)하였다.

• Journal of Plant Biotechnology
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• 제5권4호
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• pp.239-244
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• 2003

A protocol has been developed for rapid large scale clonal propagation of an aromatic endangered medicinal plant, Hemidesmus indicus R. Br. with the elimination of the problems such as premature leaf fall and callus formation during caulogenesis and rhizogenesis. Multiple shoots were induced from shoot tip and nodal explants on Murashige and Skoog (MS) medium supplemented with 1 mg/L Benzylaminopurine (BAP) and 0.5 mg/L Napthaleneaceticacid (NAA). Addition of 15 mg/L adenine sulphate to the above medium checked leaf abscission completely, reduced the time required for caulogenesis and restored morphogenetic potential after several subcultures. The in vitro grown propagules were rooted in 1/2 MS medium supplemented with 2 mg/L Indolebutyric acid (IBA) +1 mg/L NAA and sucrose 0.7% (w/v). Addition of charcoal at 100 mg/L to the rooting medium quickened root initiation with a complete check on callus formation. The effect of sucrose concentration on both caulogenesis and rhizogenesis was also studied. The resultant plantlets were acclimatized and grown in fields where ninety eight percent of the rooted shoots survived and grew normally. The estimation of the secondary metabolite content in the shoots of the regenerated plant and the mother plant indicated that the concentration of the three secondary metabolites lupeol, vanillin and rutin was similar.

• 생약학회지
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• 제26권1호
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• pp.40-46
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• 1995

A methanol extract of the herbs of Lactuca indica L. effectively decreased the serum levels of total cholesterol and LDL-cholesterol when orally administered with diet. On fractionation of the extract, a chloroform-soluble fraction showed the similar effects with the methanol extract. Chromatographic separation afforded a mixture of triterpene alcohols and their acyl derivatives. A mixture of triterpene alcohols were identified as ${\beta}-amyrin$, ${\alpha}-amyrin$, lupeol, pseudotaraxasterol, taraxasterol and germanicol on the basis of spectroscopic methods. The acyl moieties in the corresponding acyl mixture were characterized as acetates and palmitates, respectively. And three kinds of sterol such as ${\beta}-sitossterol$, compesterol and stigmasterol were isolated as a mixture state.

• 셀메드
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• 제10권4호
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• pp.26.1-26.7
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• 2020

Sahdevi is an Indian herb commonly found in all part of the country in rainy season. It is adopted by Arabs due to its easy availability and various important pharmacological actions and uses. It is known by different vernaculars in different parts of the country such as Mahabala in Marathi, Sadodi in Gujrati, Kukseem in Bengali and in English it is called ash-colour fleabane or purple fleabane. The herb contains β-amyrin, lupeol, β-sitosterol, stigmasterol, α-spinasterol, phenolic resin and potassium chloride etc. It contains Dafi'-i Hummiyat (antipyretic) Muqawwi-i Badan (general tonic) and Musaffī-i-Dam (blood purifier) actions and used for management of many disease conditions. It has been used for the management of chronic fevers like fever associated with tuberculosis. It is a priceless herb with important pharmacological action and can be used effectively in place of costly drugs.

• Advances in Traditional Medicine
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• 제9권3호
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• pp.238-244
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• 2009

Gymnosporia rothiana (walp) Lawson (celastraceae), commonly known as Maytenus rothiana, is used in Indian folk medicine as an antiulcerogenic agent. However, there have been no scientific reports regarding its antiulcer activity. Therefore, this study was intended to evaluate the antiulcer property of petroleum ether, chloroform, and methanolic extract of leaves of Gymnosporia rothiana at different dose levels in ethanol induced and indomethacin induced gastric ulcer models. It was observed that oral administration of all the extract of Gymnosporia rothiana produces significant reduction in ulcer lesion index as well as increase in volume and pH of gastric content in both experimental models, being petroleum ether extract the most effective at dose of 250 mg/kg; it significantly reduced gastric lesion index (70.06%), in comparison to omeprazole (71.20%) and methanolic extract at a dose of 500 mg/kg (67.22%). Increased gastric mucosal defense mechanism by petroleum ether extract is probably due to its high levels of terpenoids like $\beta$ amyrin, lupeol acetate. The present results clearly shows antiulcer effect of Gymnosporia rothiana against various irritants has been mainly due to cytoprotective effect mediated through prostaglandin and partly due to free radical scavenging activity.

• 생약학회지
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• 제46권2호
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• pp.93-98
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• 2015

Eleven compounds, lupenone (1), lupeol (2), stigmasterol (3), β-sitosterol (4), 24-methylene-3,4-seco-cycloart-4(28)-en-3-oic acid (5), 24-methylene-3,4-seco-cycloart-4(28)-en-3-methyl ester (6), (+)-(1S,4R)-7-hydroxycalamenene (7), hibicuslide C (8), isopropyl-${\beta}$-D-glucopyranoside (9), syringaresinol-4'-O-${\beta}$-D-glucoside (10), and rutin (11) were isolated from the aerial parts of Abutilon theophrasti. The chemical structures of compounds 1-11 were determined by the basis of physicochemical properties and spectroscopic methods such as 1D and 2D NMR. These compounds were isolated from this plant for the first time. In addition, compounds 6 and 9 were obtained for the first time as natural products not as synthetics.

• 셀메드
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• 제9권4호
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• pp.4.1-4.5
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• 2019

The medicinal plants are extensively used for curing variegated malady in day to day life. There is an emergent demand for plant based medicines, food supplements, health products, pharmaceuticals etc. Mochrus (Bombax ceiba Linn.) is one of the valuable medicinal plants used in Unani system of medicine since relic belongs to family Bombacaceae. It is a tall tree and widely distributed through India, Africa, Australia and tropical Asia. Many parts of the plant (root, stem bark, gum, leaf, prickles, flower, fruit, seed and heartwood) are used for the treatment of a variety of ailments. It is reported to possess nafe sailanur reham (beneficial in leucorrhea), mujaffif (siccative), muqawwi reham (uterine tonic), qabiz (constipative), muallide mani (production of semen), mumsik wa mughalliz mani (increase consistency of semen), dafe fasaad khoon wa safra (purifies blood and bile) etc. It is used in asthma, diarrhoea, wound, leprosy, boils and many other skin diseases. Also possess some important pharmacological activities such as antioxidant, analgesic, antipyretic, antibacterial, diuretic, hepatoprotective, anticancer, hypoglycaemic and hypotensive etc. It is reported to contain phytoconstituents like polysaccharides, naphthoquinones, anthocyanins, lupeol and naphthol etc. This paper provides a compendium review on pharmacological, phytochemical properties and therapeutic benefits of the plant.

• Natural Product Sciences
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• 제24권4호
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• pp.219-224
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• 2018

During the screening for cytotoxic compounds from plants grown in Korea, Betula platyphylla (BP) showed potent activity against the adenocarcinomic human alveolar basal epithelial A549 cell line. To identify the cytotoxic components from BP, the $CH_2Cl_2$ fraction with the most significant cytotoxic effect was applied to the column chromatographies. Seven compounds were isolated: lupeol (1), betulinic acid (2), (-)-rhododendrol (3), platyphyllenone (4), platyphyllone (5), (-)-centrolobol (6), and oleanolic acid (7). Among them, three diarylheptanoids (4 - 6) exhibited cytotoxicity toward A549 cells. Especially, $50{\mu}M$ of 4 reduced A549 cell viability to $18.93{\pm}0.82%$ compared to control ($100.00{\pm}21.48%$). Lactate dehydrogenase (LDH) leakage and intracellular reactive oxygen species (ROS) production were also induced by $50{\mu}M$ 4. This is the first report on the cytotoxic effect of BP-derived diarylheptanoids 4-6 against A549 cells. The compound 4 may be useful for the development of early hit compounds for non-small cell lung carcinoma, but the consideration about selectivity of 4 is required since 4 also showed the cytotoxicity in the human normal lung epithelial BEAS-2B cell line.