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http://dx.doi.org/10.3839/jabc.2022.035

Studies on the effect of Betula platyphylla extract on human dermal papilla cell proliferation and its mechanism of action  

Seunghyun Ahn (Department of Applied Chemistry, Dongduk Women's University)
Jung Yeon Lee (Department of Applied Chemistry, Dongduk Women's University)
Eunbi Hong (Division of Applied Chemistry and Cosmetic Science, Dongduk Women's University)
Jiyun Kim (Division of Applied Chemistry and Cosmetic Science, Dongduk Women's University)
Won Seok Jeong (RNS Inc. R&D Center)
Kown Ki Moon (VICHE Cosmetic R&D Center)
CheongTaek Kim (RNS Inc. R&D Center)
Jiha Sung (Division of Applied Chemistry and Cosmetic Science, Dongduk Women's University)
Seyeon Park (Division of Applied Chemistry and Cosmetic Science, Dongduk Women's University)
Publication Information
Journal of Applied Biological Chemistry / v.65, no.4, 2022 , pp. 269-275 More about this Journal
Abstract
Betula platyphylla extract includes various materials which showed biological activity such as terpenoids. For this reason, Betula platyphylla extract has been used to alleviate inflammation. In this study, extract of Betula platyphylla was obtained and purified using several solvents and evaluated whether they showed effect on prevention of hair loss. Cell cytotoxicity assay was performed to investigate the effect of extracts on cell proliferation. Western blotting was performed to observe the changes in expression of several related growth factors such as β-catenin, VEGF, IGF1, and cyclin D. Also, 5-α-reductase activity was measured. The ethyl acetate extract was divided into four partial extracts and named as H3-1, H3-2, H3-3, and H3-4. The H3-2 extract showed proliferation activity of human derma papilla cell and increased the protein expression of several related growth factors such as β-catenin, VEGF, IGF1, and cyclin D, comparable to the effect of Ethyl 3,4,5-Trimethoxy Benzoate (ETB)and Lupeol (LPO). Moreover, we found that the fraction H3 was shown to decrease 5-α-reductase activity while ETB and LPO had no significant effect on 5-α-reductase activity.
Keywords
5-${\alpha}$-reductase activity; Betula platyphylla extract; Ethyl 3,4,5-trimethoxy benzoate; Hair growth; Human derma papilla cell (HDP); Lupeol;
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Times Cited By KSCI : 2  (Citation Analysis)
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