• Journal of the Korean Chemical Society
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• v.46 no.2
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• pp.145-150
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• 2002

A Method to determine Cr(III)ion in aqueous solution by chemiluminescence method using a stopped flow system has been studied. The method is based on the increased chemiluminescence intensity with the addition of Cr(III) to a solution of lucigenin a nd hyrogen peroxide. The effects of pH, injection volumes of reagent and sample, and concentration of lucigenin and hyrogen peroxide on the chemiluminescence intensity have been investigated. The calibration curve for Cr(III) ion was linear over the range from 1.0${\times}$$10^{-6}$ to 1.0${\times}$$10^{-3}$M and the detection limit was 5.2${\times}$$10^{-8}$M under the optimal experimental condition of 437nm, 12.8,and 1.0${\times}$$10^{-6}$ and 2.0M for emission wavelength, pH, and concentration of lucigenin and hyrogen peroxide, respectively.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.11a
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• pp.103-108
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• 2003

A method to determine chromium(III) ion in aqueous solution by chemiluminescence method using a lucigenin entrapped silica sol-gel film has been studied. An optical probe for chromium(III) ion has been prepared by entrapping lucigenin into silica sol-gel film coated on a glass support by dip coating. The chromium(III) optical sensor is based on the catalytic effect of chromium(IIII) ion on the reaction between lucigenin and hydrogen peroxide in basic solutions. The effects of Nafion, DMF and Triton X-100 were investigated to find the optimum condition to minimize cracking and leaching from the probe. The effects of pH and concentrations of lucigenin and hydrogen peroxide on the chemiluminescence intensity were investigated. The chemiluminescence intensity was increased linearly with increasing chromium(III) concentration from $2.5{\times}10^{-4}$M to $8.0{\times}10^{-7}$M and the detection limit was $4.0{\times}10^{-7}$M.

• Analytical Science and Technology
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• v.21 no.1
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• pp.20-24
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• 2008

A chemiluminescence method has been developed to determine vitamin $P_1$ in aqueous solution which is based on the enhancement of the intensity of lucigenin using lucigenin-$H_2O_2$ as chemiluminogenic system. The effects of experimental parameters such as concentrations of lucigenin, pH and concentrations of $H_2O_2$ were studied. The present method allows the determination of vitamin $P_1$ over the range $7.5{\times}10^{-6}{\sim}5.0{\times}10^{-4}mol/L$. The detection limit was $5.7{\times}10^{-7}mol/L$. The relative standard deviation was 0.75 % for 10 determinations of $7.5{\times}10^{-5}mol/L$ vitamin $P_1$. The correlation coefficient of the working curve was 0.9984 (S/N=3).

• Korean Journal of Pharmacognosy
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• v.29 no.4
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• pp.391-395
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• 1998

The phagocytic activity of murine peritoneal macrophages was determined by lucigenin chemiluminescence with luminometer and engulfment of fluorescein-conjugated E. coli particles. 70% MeOH extract of Lithospermi Radix was fractionated successively with hexane, methylene chloride, n-BuOH and water. The water fraction (m.w. 500 to 1,000) enhanced the lucigenin chemiluminescence and the engulfment of fluorescein-conjugated E. coli particles in murine peritoneal macrophages. The water fraction suppressed the production of nitric oxide in the macrophages. These results suggest that an active fraction of phagocytosis in Lithos-permi Radix is the water fraction and the molecular weight is 500 to 1,000.

• BMB Reports
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• v.28 no.6
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• pp.533-537
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• 1995

The detection with lucigenin under physiological conditions is selective for ${\cdot} O_{2}^{-}$, for it can be accepted that lucigenin indicates actual intramembranal $\cdot O_{2}^{-}- formation$. Lucigenin chemiluminescence (CL) was elicited from the plasma membrane (PM) only by addition of reduced pyridine nucleotide. NADPH was preferred to NADH in PM and hepatocytes. This specificity was masked by $NAD(P)^+$ inhibition. The half maximum rate of CL increase was obtained with 1.5 ${\mu}m$ NADH or 55 ${\mu}m$ NADPH in hepatocytes and 6 ${\mu}m$ NADH or 30 ${\mu}m$ NADPH in plasma membranes. Measurement of these NADPH values required the presence of a NADPH-regenerating system. With NADPH the maximal rate obtained was 10 fold higher than with NADH. NADPH and NADH could produce CL when having access from either side of the membrane. They seemed to react with the identical acceptor because NADH-induced CL was also inhibited by $NADP^+$. The characteristics of ${\cdot}O_{2}^{-}-formation$ produced by pyridine nucleotide will be discussed.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.11a
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• pp.109-112
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• 2003

A method to determine As(V) ions in aqueous solution by chemiluminescence method has been studied using a stopped flow system. The method is based on the increased chemiluminescence intensity with the addition of As(V) ion to a solution of lucigenin and hydrogen peroxide. The effects of KOH concentration, $H_2O_2$ concentration and flow rate of reagents on the chemiluminescence intensity have been investigated. The calibration curve for As(V) was linear over the range from $1.0{\times}l0^{-6}$M to $1.0{\times}l0^{-4}$M, the coefficient of correlation was 0.997 and the detection limit was $3.3{\times}l0^{-7}$M under the optimal experimental conditions.

• YAKHAK HOEJI
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• v.42 no.6
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• pp.567-571
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• 1998

The phagocytic activity of murine peritoneal macrophage, was determined by lucigenin chemiluminescence and engulfment of fluorescein-conjugated E. coli particle. The acti vity-guided fractionation upon the methylenechloride fraction of Aurantii immaturi pericarpium led to the isolation of a flavonoid, isosinensetin, as a suppressive component of phagocytosis. Isosinensetin suppressed the lucigenin chemiluminescence and the engulfment of fluorescein-conjugated E. coli particles and enhanced the production of nitric oxide in murine peritoneal macrophage.

• Korean Journal of Animal Reproduction
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• v.26 no.3
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• pp.275-289
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• 2002

The objective of this study was to develop an in vitro assessment of sperm fertilizing capacity of bulls and investigate the factors influencing sperm function and characteristics of frozen-thawed bovine spermatozoa. in vitro fertilization (IVF), the evaluation of motility and normal morphology, HOST (hypoosmotic swelling test), Ca-ionophore induced acrosome reaction, luminol and lucigenin-dependent chemiluminescence for the measurement of reactive oxygen species (ROS), the measurement of malondialdehyde formation for the analysis of lipid peroxidation (LPO), and the evaluation of DNA fragmentation using the method of 747-mediated nick end labelling (TUNEL) by flow cytometry were performed in frozen-thawed bovine spermatozoa. Correlations between the rates of fertilization, blastocyst formation after IVF and the values of respective assays were investigated. 1. IVF rate and blastocyst formation rate averaged 64.4% and 34.3% for spermatozoa from high -fertility bull group and averaged 18.5% and 6.2% for spermatozoa from low-fertility bull group, respectively. There were significantly different between two bull groups. Sperm motility and percentage acrosome reaction averaged 79.0% and 66.2% for spermatozoa from high-fertility bull group and averaged 40.7% and 22.9% for spermatozoa from low-fertility bull group, respectivitely. There were not different between two bull groups. 2. Luminol depenent chemiluminescence, LPO and DNA fragementation averaged 6.4, 2.0 nmol and 2.6% from spermatozoa from high-fertility bull group and averaged 6.5, 3.1 nmol and 7.4% for spermatozoa from low-fertility bull group, respectively. There were significantly different between two bull groups. There was no significant difference in lucigenin dependent chemiluminescence between two bull groups. 3. Fertilization rate was positively correlated with motility and the rate of Ca-ionophore induced acrosome reaction, but negatively correlated with the frequency of luminol-dependent chemiluminescence, the rate of LPO, and the percentage of sperm with DNA fragmentation. There was no correlation between fertilization rate and the percentage of swollen spermatozoa, normal morphology, and the frequency of lucigenin-dependent chemiluminescence. 4. Blastocyst formation rate was positively correlated with the rate of Ca-ionophore induced acrosome reaction, but negatively correlated with the frequency of luminol-dependent chemiluminescence, the rate of LPO, and the percentage of sperm with DNA fragmentation. There was no correlation between blastocyst formation rate and motility, the percentage of swollen spermatozoa, normal morphology, and the frequency of lucigenin-dependent chemiluminescence. In conclusion, these data suggest that ROS significantly impact semen quality. The assays of this study may provide a basis fur improving in vitro assessment of sperm fertilizing capacity.

• Fisheries and Aquatic Sciences
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• v.6 no.4
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• pp.209-212
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• 2003

Lucigenin (Lg)- and luminol (Lm)-dependent chemiluminescence (CL) was used to compare the respiratory burst of rockfish (Sebastes schlegeli) phagocytes after stimulation with phorbol myristate acetate (PMA). To establish which reactive oxygen species (ROS) contributes to the observed CL, the modulators of ROS metabolism, such as superoxide dismutase (SOD), catalase, and sodium azide $(NaN_3)$ were used. Although LgCL responses were inhibited significantly by the addition of either SOD or catalase, in comparison to the control, significantly lower LgCL responses were recorded by SOD than catalase. LmCL also showed significantly decreased responses by the addition of SOD and catalase. However, there were no statistical differences in CL responses between SOD and catalase additions. More profound and significant decrease of LmCL responses were recorded by simultaneous addition of SOD and catalase. Sodium azide markedly enhanced LgCL responses, while it significantly inhibited LmCL responses. These results indicate that LgCL and LmCL can be used to measure extracellular $O_2$ production and myeloperoxidase (MPO)-mediated ROS production in fish phagocytes, respectively. Furthermore, LmCL can be used for analyzing intracellular ROS production by simultaneous addition of both SOD and catalase.

• The Journal of Korean Medicine
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• v.19 no.2
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• pp.420-438
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• 1998

Herba Xanthii(HX), Radix Xantluii(RX) and Fructus Xanthii(FX) is one of the oriental medicine that has been used for the treatment of such infectious diseases and tumors. However, the mechanism of the drug is not investigated much. This study was done to know the effects of HX, RX and FX extract on the such innate immunities as phagocytic function and reactive radical formtions from phagocytes and the such acquired immunities as humoral and cell-mediated immunities. The followings are the results obtained from this study: 1. HX2 and FX1 groups increases the in vivo phagocytic activity of mononuclear phagocytes. 2. HXB, RXB, RXC, FXB and FXC groups increase the in vitro phagocytic activities. 3. RXB group stimulated the macrophages to produce nitric oxide in the presence of $interferon-{\gamma}$ $(IFN-{\gamma})$. 4. HX and RX whole groups increased the luminol-amplified reactive oxygen intermediate production in vivo. 5. HX whole and RX1, FX2 groups increased the lucigenin-amplified reactive oxygen intennediate production in vivo. 6. HXC group only increased the luminol-amplified reactive oxygen intermediate production in vitro. 7. HXB, FXB and FXC groups increased the lucigenin-amplified reactive oxygen intermediate production in vitro. 8. HX2, RX1 and FX whole groups increased the hemolysin formations from B cells. 9. HX, RX and FX whole groups significantly increased the rosette forming cells from the spleen. 10. HX, RX and FX whole groups significantly decreased the delayed-type hypersensitivity measured by footpad swelling. The above results demonstrate that HX, RX and FX has enhancing effects on innate immunity selectively and decreasing effects on delayed-type hypersensitivity of cell-mediated immunity according to medicinal part and diluted condition. This immunomodulating effects of HX, RX and FX might be responsible for the treatment of immune-mediated disorders.