• Title/Summary/Keyword: low-temperature induction

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Antioxidant Enzyme Activity and Cell Membrane Stability of Korean Bermudagrass Genotypes Different in Ploidy at Dormant Stage (배수성이 다른 자생 버뮤다그래스의 휴면 전후 항산화 효소활성 및 세포막 안정성 변화)

  • Lee, Geung-Joo;Lee, Hye-Jung;Ma, Ki-Yoon;Jeon, Young-Ju;Kim, In-Kyung
    • Asian Journal of Turfgrass Science
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    • v.25 no.1
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    • pp.17-21
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    • 2011
  • Korean bennudagrass collections showed diverse genetic variations in their morphology, growth habit, and cytological aspects. Chromosome number and nuclear DNA content of the bennudagrasses indicated a ploidy level ranging from triploid (2n=3x) to hexaploid (2n=6x). In this study, we investigated the different responses of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, ascorbate peroxidase) and cell membrane stability of those bennudagrass cytotypes to lower temperature and shorter day length, which meets a dormant induction in Korea. All the antioxidant enzymes were found to be higher during dormant stage, while the heme-containing catalase which converts hydrogen peroxide ($H_2O_2$) to water and oxygen molecules was activated before dormant initiation in the three cytotypes except for hexaploid bennudagrass. The triploid and tetraploid which exhibited relatively finer leaves and a rapid establishment speed were found to show increased activities of superoxide dismutase and peroxidase enzyme. The malondialdehyde(MDA) which is a product of lipid peroxidation in the cell membrane damaged by the hydroxyl radical was increased in all cytotypes as temperature declined, and tri- and tetraploids which had more protective antioxidant enzymes demonstrated a significantly lower MDA production. Similarly electrolyte leakage was higher in penta- and hexaploidy, seemingly more damage to cell membrane when low temperature was implemented. Results indicated that antioxidant responses of different cytotypes were genetically specific, which needs to be investigated the relevance with the low temperature tolerance in the bermudagrass further at the molecular level.

Practical Usage of Low-Temperature Metal Catalyst for the Destruction of Volatile Organic Compounds (VOCs) (휘발성 유기화합물(VOCs) 제거를 위한 저온금속촉매 실용화에 관한 연구)

  • Jung, Sung-Chul;Lee, Seung-Hwan
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.6
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    • pp.397-405
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    • 2012
  • In this study, performance evaluation of newly developed technology for the economical and safe removal of volatile organic compounds (VOCs) coming out from electronic devices washing operation and offensive odor induction materials was made. Metal oxidization catalyst has shown 50% of removal efficiency at the temperature of $220^{\circ}C$. Composite metal oxidization catalyst applied in this study has shown that the actual catalysis has started at the temperature of $100^{\circ}C$. Comprehensive analysis on the catalyst property using Mn-Cu metal oxidization catalyst in the pilot-scale unit was made and the removal efficiency was variable with temperature and space velocity. Full-scale unit developed based on the pilot-scale unit operation has shown 95% of removal efficiency at the temperature of $160^{\circ}C$. Optimum elimination effective rates for the space velocity was found to be $6,000hr^{-1}$. The most appropriate processing treatment range for the inflow concentration of VOCs was between 200 ppm to 4,000 ppm. Catalyst control temperature showed high destruction efficiency at $150{\sim}200^{\circ}C$ degrees Celsius in 90~99%. External heat source was not necessary due to the self-heat reaction incase of VOCs inflow concentration is more than 1,000 ppm. Equipment and fuel costs compared to the conventional RTO/RCO method can be reduced by 50% and 75% respectively. And it was checked when there was poisoning for sulfide and acid gas.

Germination Induction of Some Weed Seeds Stored Under the Dry and Low Temperature Condition (건조저온저장(乾燥低溫貯藏)한 몇가지 잡초종자(雜草種子)의 발아유기(發芽誘起))

  • Cho, K.Y.;Kim, J.S.;Kim, Y.S.
    • Korean Journal of Weed Science
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    • v.7 no.1
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    • pp.19-28
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    • 1987
  • These experiments were conducted to recognize the germination change in relation to storage period, using 25 kinds of field-collected weed seeds stored under the dry and low temperature ($4{\pm}1^{\circ}C$), and to establish a simple germination induction methods in some kinds of them. No or weak dormancy was observed in Lepidium virginicum, Rumex japonicus, Cirsium japonicum var. ussuriense, Aeschnomene indica and Ludwigia prostrate. The germination of Digltaria sanguinalis, Portulaca oleracea, Amaranthus spp., Echinochloa crux-galli, Polygonum persicaria, Cyperus iria, and Aeschynnmene indica was enhanced by $H_2SO_4$ treatment for 20, 2, 2, 50, 40, 3 or 5 minutes, respectively. Favorable germination in Polygonum persicaria and Echinochloa crus-galli was obtained by soaking in water for 25 days and 50 days, respectively. Moisture treatment for 21 days and 14 days caused the enhancement of germination in Cyperus difformis and Aneilema keisak, respectively. Combination of various treatments in Cyperus iria was most effective when the seeds treated with $H_2SO_4$ for 3 minutes incubated in alternating temp. after soaking in 0.2% $KNO_3$, but their effect on Digitaria sanguinnlis was little.

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Effects of water temperature and salinity on the formation of prezoosporangia and zoosporangia of the protozoan parasite, Perkinsus olseni, isolated from the Manila clam Ruditapes philippinarum on the west coast of Korea (퍼킨서스편모충 (Perkinsus olseni) 의 휴면포자와 유주자 형성에 수온과 염분이 미치는 영향)

  • Kim, Hyon-Joong;Bang, In-Seok;Park, Kyung-Il
    • The Korean Journal of Malacology
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    • v.26 no.3
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    • pp.211-215
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    • 2010
  • The genus Perkinsus are parasitic protozoans that cause massive inflammatory responses in infected marine shellfish worldwide. This ultimately leads to great economic losses. This study examined the effects of water temperature and salinity on the formation of prezoosporangia and zoosporangia in order to understand the ecology of the pathogens. The induction of prezoosporangia from trophozoites occurred readily at higher water temperatures (20 and $30^{\circ}C$) and they had larger diameters than those incubated at lower temperatures (4 and $10^{\circ}C$). The formation of zoospores in prezoosporangia was also strongly influenced by water temperature and salinity; prezoosporangia exposed to water temperatures of 20 and $30^{\circ}C$ and salinities of 20 and 30 ppt had high rates of zoosporulation, while no or very low rates of zoosporulation were observed at temperatures below $10^{\circ}C$ or salinity below 10 ppt. Our data will be useful for the development of strategies to counter P. olseni proliferation in Korean waters.

CHILLING SENSITIVITY OF CUCUMBER PLANTS MONITORED IN TERMS OF CHLOROPHYLL FLUORESCENCE

  • Kang, In-Soon;Moon, Byoung-Yong;Seo, Kye-Hong;Chun, Hyun-Sik;Lee, Chin-Bum
    • Journal of Photoscience
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    • v.3 no.1
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    • pp.9-14
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    • 1996
  • For three cultivars of chilling-sensitive cucumber plants, chilling sensitivity was evaluated in terms of photosynthetic activity using Chl fluorescence techniques. Low-temperature treatment caused a decrease in photosynthetic activities of cucumber leaves, measured as CO$_2$ exchange, as well as the decrease in the stomatal conductance. FR of the three cultivars decreased after chilling for 24 h in light and the extent of decline of F$_R$ was the greatest in 'Chosaeng' cultivar. When these plants were recovered from light-chilling, 'Chosaeng' and 'Samchuk' cultivars did not fully restore the original value of F$_R$ after 24 h of recovery, in contrast to 'Ilmi' cultivar which showed a rather efficient recovery. The results of FR study showed that 'Chosaeng' was most susceptible, whereas Ilmi was most resistant, to chilling among the three cultivars of cucumber plants. When quenching coefficients for chlorophyll fluorescence was analyzed after chilling the cucumber plants for 24 h in light, 'Chosaeng' elicited more rapid declines in the coefficients for photochemical quenching (qQ), non-photochemical quenching (qNP) and energy-dependent quenching (qE) than 'Ilmi' and 'Samchuk'. The implications of these observations are discussed in relation to the growth habits of the respective cultivars in the field. The results showed that measurement of chlorophyll fluorescence was an effective means of screening chilling tolerance of cucumber plants. Furthermore, the study on the chlorophyll fluorescence induction and fluorescence quenching charactersitics showed that low temperature could accelerate inhibition of photosynthesis in chilling-sensitive plants, by limiting Calvin cycle activity and disrupting, in part, the energy dissipation mechanims of the photosystem II.

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Induction of antioxygenic enzymes as defense systems in plant cells against low temperature stress : (I) Accumulation of pyruvate in cells during cold treatment and activation of antioxygenic enzymes during post-chilling period (식물의 냉해에 대한 생체방어기구로서 항산소성 효소의 유도 : (1) 저온처리중 pyruvate의 세포내 축적과 상온환원후 항산소성 효소의 활성화)

  • Kim, Jong-Pyung;Hahn, Chang-Kyun;Jung, Jin
    • Applied Biological Chemistry
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    • v.34 no.2
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    • pp.162-167
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    • 1991
  • In an attempt to explore the mechanistic aspects of chilling injury in plants and their defensive measures against the low temperature stress, the time sequential measurements of pyruvate, superoxide radicals$(O_{\overline{2}})$ and antioxygenic enzymes during whole period of injury-inducing treatment were performed using mostly rice seedlings. Pyruvate was substantialy accumulated in leaf tissues during the exposure period to $5^{\circ}C$ of the seedlings ; the relative extent of the accumulation was increased with increasing time of the cold treatment. When the cold-treated plants were translocated to ambient temperature$({\sim}25^{\circ}C)$, the accumulation started to dissipate, concomitantly accompaning a remarkable increase in the $O_{\overline{2}}$ level of tissues. Superoxide dismutase(SOD) and catalase were also activated during post-chilling period, although they showed a considerable lag time for activation. In contrast, glutathione peroxidase, another antioxygenic enzyme in cells, was not activated at all by preceding cold treatment of plants. The uptake of exogenous $O_{\overline{2}}$ by the roots of rice seedlings resulted in increase in the activities of SOD and catalase in root tissues. The supply of $H_2O_2$ to plan st brought about the activation of catalase in situ, while failing to exert any effect on the activation state of glutathione peroxidase. The results obtained in this work suggest that pyruvate accumulation in cells is the direct cause of the overproduction of $O_{\overline{2}}$ and thereby other toxic activated oxygen species, and that SOD and catalase may play a crucial role in the protection of plant cells against active oxygen-mediated chilling injury.

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A Study on the Development of High Permeability and Low Coercivity Ni-Zn Ferrite (고투자율, 저보자력을 갖는 Ni-Zn Ferrite의 개발에 관한 연구)

  • 고재귀
    • Journal of the Korean Magnetics Society
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    • v.7 no.1
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    • pp.13-18
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    • 1997
  • The effects of the various raw material composition and sintered temperature on the physical properties of Ni-Zn ferrite have been investigated. They turned out to be spinel structure by X-ray diffraction and the size of grain from microscope was from 6 ${\mu}{\textrm}{m}$ to 16 ${\mu}{\textrm}{m}$. As the sintering temperature was increased from 1030 $^{\circ}C$ to 1070 $^{\circ}C$, the initial permeability and magnetic induction has increased and the both of Q factor and coercive force has decreased. The coercive force and curie temperature were almost the same at each specimen. Their values were about 0.20 Oe and 220 $^{\circ}C$. The frequency of specimen will used in the range from 400 kHz to 20 MHz. The basic composition of $Ni_{0.14}Zn_{0.64}Cu_{0.22}Fe_2O_4$ (specimen B) sintered at 1050 $^{\circ}C$ shows the best results at magnetic induction($B_r & B_m$).

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Synthesis of high purity carbon powders using inductively thermal plasma (유도 열플라즈마 공정을 이용한 고순도 카본분말 합성)

  • Kim, Kyung-In;Han, Kyu-Sung;Hwang, Kwang-Taek;Kim, Jin-Ho
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.23 no.6
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    • pp.309-313
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    • 2013
  • Silicon carbide (SiC) has recently drawn an enormous industrial interest because of its useful mechanical properties such as thermal resistance, abrasion resistance and thermal conductivity at high temperature. Especially, high purity SiC is applicable to the fields of power semiconductor and lighting emitting diode (LED). In this work, high purity carbon powders as raw material for high purity SiC were prepared by a RF induction thermal plasma. Dodecane ($C_{12}H_{26}$) as hydrocarbon liquid precursor has been utilized for synthesis of high purity carbon powders. It is found that the filtercollected carbon powders showed smaller particle size (10~20 nm) and low crystallinity compared to the reactor-collected carbon powders. The purities of reactor-collected and filter-collected carbon powders were 99.9997 % (5N7) and 99.9993 % (5N3), respectively. In addition, the impurities of carbon powders synthesized by RF induction thermal plasma were mainly originated from the surrounding environment.

Anther Culture Efficiency According to Plating Method in Naked Barley (쌀보리 약배양을 위한 약치상 방법별 배양효율)

  • Park, Tae-Il;Jeoung, Sun-Ok;Kim, Young-Jin;Kim, Hyun-Soon;Seo, Jae-Hwan;Park, Ki-Hun;Kim, Jung-Gon;Yun, Song-Joong
    • Journal of Plant Biotechnology
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    • v.34 no.3
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    • pp.237-242
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    • 2007
  • Barley anther culture is hard working to plating picking out anther from the glume and demand long time comparing to be short available development stage for effective culture. Also, it has been treatment massive materials due to low plantlet comparing to get desirable plants intensively. Consequently, this experiment was carried out trying to be more high barley anther culture effectively in terms of save plating effort. Plating materials and culture temperature affected anther culture efficiency are among the inoculation tissues or organs such as anthers, spikelets and whole panicles, culture efficiency was higher with spikelets in two-rowed than six-rowed barley due primarily to a lower contamination, and calli were induced within 30 to 50 days. Callus induction and plant regeneration rates were higher in cultures at $25^{\circ}C$ than at $15^{\circ}C$ and $20^{\circ}C$. Days to callus induction were 25 to 50 days at $25^{\circ}C$ and 50 to 60 days at $20^{\circ}C$.

Effect of Melt-Spinning Process on Hydrogen Storage Properties of Mass-Produced Ti0.85Zr0.13(Fex-V)0.56Mn1.47Ni0.05 Alloy (대량용해 Ti0.85Zr0.13(Fex-V)0.56Mn1.47Ni0.05 수소저장합금의 용융방사공정을 통한 수소저장특성)

  • Kim, Jinho;Han, Kyusung
    • Journal of Hydrogen and New Energy
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    • v.24 no.5
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    • pp.367-372
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    • 2013
  • Hydrogen storage as a metal hydride is the most promising alternative because of its relatively large hydrogen storage capacities near room temperature. TiMn2-based C14 Laves phases alloys are one of the promising hydrogen storage materials with easy activation, good hydriding-dehydriding kinetics, high hydrogen storage capacity and relatively low cost. In this work, multi-component, hyper-stoichiometric $Ti_{0.85}Zr_{0.13}(Fe_x-V)_{0.56}Mn_{1.47}Ni_{0.05}$ C14 Laves phase alloys were prepared by a vacuum induction melting for a hydrogen storage tank. Since pure vanadium (V) is quite expensive, the substitution of the V element in these alloys has been tried and some interesting results were achieved by replacing V by commercial ferrovanadium (FeV) raw material. In addition, the melt-spinning process, which was applied to the manufacturing of some of these alloys, could make the plateau slopes much flatter, which resulted in the increase of reversible hydrogen storage capacity. The improvement of sloping properties of melt-spun $Ti_{0.85}Zr_{0.13}(Fe_x-V)_{0.56}Mn_{1.47}Ni_{0.05}$ alloys was mainly attributed to the homogeneity of chemical composition.