• 자연과학논문집
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• 제13권1호
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• pp.83-96
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• 2003

Effect of Nutrient Concentrations, fertigation frequency, and learching percentage on crop growth and nutrient concentrations in root media were evaluated. The treatment of each irrigation with $50 mg.L^{-1}$ of nitrogen in stage 2 and increase to $80 mg.L^{-1}$ nitrogen in stage 3 had the highest crop growth at 34 days after sowing among treatments tested. Feeding with low nutrient concentrations and elevated frequency decreased crop growth. In treatments of each leaching percentage, feeding with low nutrient concentrations and elevated frequency resulted in increased tissue nutrient contents. The less tissue potassium content and higher calcium and magnesium contents were observed in treatment of 50% leach than those in 0% leach. All treatment tested had soil solution pH higher than 6.8. Electrical conductivity in treatments of 50% leach were lower than those of 0% leach. Feeding with low nutrient concentrations and elevated feeding frequency in each leaching percentage resulted in increased electrical conductivity in soil solution of root media. Trends of medium nutrient concentrations were similar to those of electrical conductivity.

• 생물환경조절학회지
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• 제7권1호
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• pp.71-77
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• 1998

본 실험은 배지의 종류 및 환경조건에 대하여 배지의 온도변화 특성을 분석하는 것을 목적으로 하였다. NFT DFT, 슬라브형 성형암면, 면상암면, 펄라이트 배지를 사용하는 순환식 양액재배시스템을 구축하였다. NFT와DFT시스템은 양액 및 재배조 내의 공기온도를 측정하였고, 고형배지는 배지온도를 측정 .비교하였다. NFT시스탬의 양액 공급유량을 3$\ell$/min, 5$\ell$/min로 할 때, 양액 온도는 주간에는 전자가 후자에 비하여 약 3t 정도 높게 나타났다. 재배조내의 공기온도도 동일한 경향을 보이며 약 2$^{\circ}C$ 정도 차이를 나타냈다. DFT 시스템의 수위를 0.8cm, 1.8cm로 할 때, 양액 온도는 주간에는 전자가 후자에 비하여 약 1-2$^{\circ}C$ 정도 높게 나타났다. 또한 재배조내의 공기 온도도 동일한 경향을 보였다. 펄라이트 배지는, 주간에 멀칭한 배지가 약 3$^{\circ}C$ 정도, 면상암면 배지도 약 3$^{\circ}C$ 정도 높게 나타났고, 슬라브형 암면배지는 주야간 멀칭한 배지가 1-4$^{\circ}C$정도 높게 나타났고. 특히 주간에 높은 온도상승을 나타냈다. 고형배지중에서 펄라이트 배지온도가 암면에 비교하여 상대적으로 야간은 낮고 주간이 높게 나타났다. 슬라브형 암면의 경우는 표면적 전체가 공기중에 노출되어 있기 때문에 다른 고형배지보다 주간의 온도상승이 크게 나타났다. 고형배지의 뿌리부분의 온도가 작물과 작물사이의 배지온도에 비하여 주간 약 3f정도 낮게 나타났으며, 야간의 경우는 거의 차이가 없었다.

• 한국식품영양학회지
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• 제27권3호
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• pp.495-506
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• 2014

This study was conducted to measure the effect of a community-based nutritional intervention program on dietary behaviors and nutrient intakes of low-income elderly women in Korea. The subject of the study was a group of 88 elderly women using the free meal service in Dong-gu, Gwangju city in Korea. The study was carried out from April to July 2009, and the pretest, application of nutrition education, and post-test were applied in each stage. This community-based nutrition intervention program consisted of snack supplement and nutrition education provided three times a week and 36 times in total. The nutrition education was led by the nutritionists and professionals using various educational media. Snack supplement included were milk, soymilk, carrot juice, bread, yogurt, and bananas. The result of pretest showed that the nutrient intakes of the subjects were much below the Recommended Nutrient Intake (RNI) level of Dietary Reference Intake for Koreans. Comparison of the dietary behavior score and nutrient intakes before and after the nutritional intervention program indicated that the food behavior score increased from 3.1 to 5.6 (P<0.001) and intake of energy, protein, carbohydrate, thiamin, niacin, vitamin C, iron, and potassium of the subjects all increased significantly (P<0.05). Mean Adequacy Ratio (MAR) of their nutrient intakes was also improved from 0.53 to 0.64 (P<0.01). These results indicated that the nutrition intervention program conducted in this study was effective in improving dietary behavior and nutrient intakes of elderly women in local community.

• Biotechnology and Bioprocess Engineering:BBE
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• 제4권1호
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• pp.21-25
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• 1999

Two low-salt complex media, bactopeptone and desalted yeast extract, were used for high density cultivation of the hyperthermophilic archaeon Sulfolobus solfataricus (DSM 1617). Bactopeptone, which has low mineral ion content among various complex media, was good for cell growth in batch cultures; the maximal cell density in bactopeptone was comparable to that in yeast extract. However, cell growth was rather poor when bactopeptone was added by the fed-batch procedure. Since several vitamins are deficient in abctopeptone, the effect of vitamins on cell growth was examined. Among the vitamins tested, pyridoxine was found to improve the growth rate of S. solfataricus. To reduce the growth inhibition caused by mineral ions, yeast extract was dialyzed against distilled water and then fed-batch cultures were carried out using a fed medium containing desalted yeast extract. Although the concentrations of mineral ions in yeast extract were significantly lowered by the dialysis whether low molecular weight solutes in yest extract are crucial for cell growth, we investigated the effect of trehalose, a most abundant compatible solute in yeast extract, on the growth pattern. Cell densities were increased and the length of the lag phase was markedly shortened by the presence of trehalose, indicating that trehalose plays an important role in the growth of S. solfataricus.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2003년도 International Meeting of the Microbiological Society of Korea
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• pp.26-33
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• 2003

We isolated and identifed culturable Arctic bacteria that have inhabited around Korean Arctic Research Station Dasan located at Ny-Alsund, Svalbard, Norway $(79^{\circ}N,\;12^{\circ}E)$. The pure colonies were inoculated into nutrient liquid media, genomic DNA was extracted, and phylogenetic analysis was performed on the basis of 16S rDNA sequences. Out of total 227 strains, 198 strains were overlapped or unidentified, and 43 bacteria were finally identified: 31 strains belonged to Pseudomonas, 7 strains Arthrobacter, two Flavobacterium sp., an Achromobacter sp., a Pedobacter sp., and a Psychrobacter sp. For isolation of diverse bacteria, we need more effective transport method than 3M petri-films, which were used for convenience of transportation that was restricted by volume. We also need to use other culture media than nutrient media. We expect these Arctic bacteria can be used for screening to develop new antibiotics or industrial enzymes that are active at low temperature.

• 한국조경학회지
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• 제27권3호
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• pp.109-113
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• 1999

The objective of this research was to develop growth media for artificial ground by blending calcined clay and coconut peat. To achieve this, aggregates of clay particles were mixed with disel oil and heated to high temperature(1150~120$0^{\circ}C$) to expand clays. The particle sizes of expanded clay were controlled to 2~5mm in diameter. Then expanded clayes were mixed with coconut peat and changes of soil physicochemical properties and their effect on plant growth of Hedera L. were determined. The infiltration rate of calcined clay was very high, but the water holding capacity, the cation exchange capacity(CEC), and the nutrient contents were low. The characteritics of coconut peat was vice verse to calcined clay. This indicates that the mixture of calcined clay and coconut peat have the better characteristics than each material. As compared to mineral soil, the infiltration rate, the water holding capacity, the CEC and the nutrient contents increased, but bulk density decreased to about 1/4. And, Hedera L. grown in the mixture of calcined clay and coconut peat(6:4, v/v) had higher plant height, longer leaf length, more total number of leaves per plant and fresh weight than that grown in mineral soil, but statistical differences were not observed between two treatments.

• 한국유기농업학회지
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• 제25권4호
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• pp.749-758
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• 2017

This Research was the standardization of nutrient sources by reviewing the possibility of cultivation and analysis of imported nutrient sources used in Pleurotus ostreatus culture. As a first result, the concentration of heavy metals was not detected or trace, and the total nitrogen content of cotton seed pomace was 6.22% in Australia, which was higher than that of Chinese or USA. As a second result of physical properties of nutrient sources, the water holding capacity of beet pulp from Egypt was higher with increasing pressure. The volume water content of US was highest at 400hpa and 500hpa in case of cottonseed meal. Third, the content of total nitrogen was the highest at pH 5.51 and the total nitrogen content was the highest at 2.45, and the C/N ratio was the highest at 28.18. Last result shows that first germination day, yield and biological effciency of all treatments were not significant differnet except T2. These results shows that australian cootton was inappropriate to Pleurotus ostreatus culture. Nevertheless, the safety and standardization of the Pleurotus ostreatus culture medium should be assured by reviewing the media low material importing season, mushroom yield and nutrient sources.

• Journal of Microbiology and Biotechnology
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• 제29권10호
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• pp.1495-1505
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• 2019

The Burkholderia cepacia complex (BCC) is capable of remaining viable in low-nutrient environments and harsh conditions, posing a contamination risk in non-sterile pharmaceutical products as well as a challenge for detection. To develop optimal recovery methods to detect BCC, three oligotrophic media were evaluated and compared with nutrient media for the recovery of BCC from autoclaved distilled water or antiseptic solutions. Serial dilutions ($10^{-1}$ to $10^{-12}CFU/ml$) of 20 BCC strains were inoculated into autoclaved distilled water and stored at $6^{\circ}C$, $23^{\circ}C$ and $42^{\circ}C$ for 42 days. Six suspensions of Burkholderia cenocepacia were used to inoculate aqueous solutions containing $5{\mu}g/ml$ and $50{\mu}g/ml$ chlorhexidine gluconate (CHX) and $10{\mu}g/ml$ benzalkonium chloride (BZK), and stored at $23^{\circ}C$ for a further 199 days. Nutrient media such as Tryptic Soy Agar (TSA) or Tryptic Soy Broth (TSB), oligotrophic media (1/10 strength TSA or TSB, Reasoner's $2^{nd}$ Agar [R2A] or Reasoner's $2^{nd}$ Broth [R2AB], and 1/3 strength R2A or R2AB) were compared by inoculating these media with BCC from autoclaved distilled water and from antiseptic samples. The recovery of BCC in water or antiseptics was higher in culture broth than on solid media. Oligotrophic medium showed a higher recovery efficiency than TSA or TSB for the detection of 20 BCC samples. Results from multiple comparisons allowed us to directly identify significant differences between TSA or TSB and oligotrophic media. An oligotrophic medium pre-enrichment resuscitation step is offered for the United States Pharmacopeia (USP) proposed compendial test method for BCC detection.

• 생물환경조절학회지
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• 제17권3호
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• pp.215-220
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• 2008

본 시험은 펄라이트 배지에서 적산일사량에 따른 급액이 장미 'Cardinal'의 생육에 미치는 영향을 고찰하기 위하여 수행하였다. 배지내의 pH는 6.0에서 6.1사이로 안정적이었고 EC는 급액보다는 배액에서 높게 나타났는데 후기로 갈수록 높아지는 경향이었다. 무기 성분은 비순환식 C. Sonneveld처방 기준으로 보면 인산과 칼리, 마그네슘이 집적됨을 알 수 있었는데, 특히 칼리와 마그네슘은 각각 기준량 195ppm, 18보다 집적됨을 알 수 있었다. 배지의 배액율은 전체적으로는 일사량이 많으면 높아지는 경향이었다. 장미 수액은 오전 9시 경부터 12시까지 흐름이 있었는데, 10시 30분에서 11시 사이에 $273g{\cdot}hr^{-1}$이었는데 일사량에 비례하여 수액흐름이 증가하지는 않았다. 양액소모량은 $250W{\cdot}hr^{-1}$에서는 50m1구에서 212.8ml로 많았는데 수량도 154.6본/10a로 많아 양액소모량이 수량에 영향을 미치는 것을 알 수 있었다. 펄라이트 배지에서 양액공급량은 11월부터 3월에 $200W{\cdot}hr^{-1}$에서 50ml를 1일 $6\sim10$회 급액하고 일사량이 많을 때는 $250W{\cdot}hr^{-1}$에서 30ml를 1일 $22\sim30$회 급액하는 것이 좋을 것으로 생각된다.

• Journal of Nutrition and Health
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• 제34권7호
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• pp.770-777
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• 2001

This study investigated nutritional status and eating behaviors among 59 nephritic patients with mild kidney malfunction in Korea. Nutritional status was measured by blood analysis and 1-3 day dietary recall and records, and eating behaviors were assessed by a questionnaire. Mean Body Mass Index(BMI) was within the normal range, while 21% and 14% of the patients were underweight and overweight, respectively. They received nutrition information mainly from doctors, nurses and mass media, but rarely from nutrition professionals. A quarter of patients skipped breakfast at least 3 times per 12% of the patients, respectively. Energy intake of 83% of the patients was less than the recommended level. Protein intakes of 56% of them were either under or over the recommended levels. Patients had low vitamin B$_2$ and calcium(<75% of the RDA) and excessive phosphorus(138% RDA) and vitamin C(170% RDA). Major food sources to absolute nutrient intakes were similar to those for the average Koreas, except for milk. Milk intake was low in our patients. Dietary quantity and quality were associated positively with BMI, albumin, and HDL-cholesterol and negatively with triglycerides and BUN. Results of this study indicate low nutritional status of the patients and, in turn a need for conducting nutritional education or counselling at regular at regular basis for the nephritic patients with mild kidney malfunction.