• Title/Summary/Keyword: low density lipoprotein(LDL)

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Antioxidant Activity of Aspergillus oryzae A-5 on Oxidation of Low Density Lipoprotein (Aspergillus oryzae A-5로부터 Low Density Lipoprotein(LDL)의 산화에 대한 항산화 효과)

  • Ryu, Beung-Ho;Kim, Dong-Suck;Cho, Kyung-Ja;Lee, Hong-Su;Jin, Sung-Hyun
    • Journal of Life Science
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    • v.7 no.4
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    • pp.289-296
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    • 1997
  • Antioxidative activity of fraction extracted from cultivation of Aspergillus sp. A-5 against oxidation of human low density lipoprotein(LDL) was investigated. Fractions of Aspergillus sp. A-5 cultivation was sucessively purified with ethyl acetate and silica gel column chromatography. The concentration of fraction 4 inhibited Cu$^{2+}$-induced oxidation of LDL almost completely. Band 3 isolated by the further purification of fraction 4 was higher than that of same concentration of $\alpha$-tocopherol, BHA and BHT. The elcetrophoretic mobility of oxidized LDL by addition of Band 3 was faster than that of native LDL, but slower than that of oxidzed LDL. It is concluded that fraction of Aspergillus cultivation contained antioxidants with the capacity to inhibit oxidative modification of LDL.

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Antioxidative Activity of Extracts of Aged Black Garlic on Oxidation of Human Low Density Lipoprotein (숙성 흑마늘 추출물의 Low Density Lipoprotein (LDL)의 항산화 효과)

  • Yang, Seung-Taek
    • Journal of Life Science
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    • v.17 no.10
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    • pp.1330-1335
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    • 2007
  • This study was developed to assess the antioxidative activity of aged black garlic extract on lipid peroxidation and low density lipoprotein (LDL). Antioxidative activity of aged black garlic extract on human low density lipoprotein (LDL) was investigated by monitoring a barbituric acid reactive substance (TBARS). Electron donating ability (EDA) of aged black garlic, ethanol extract was higher than that alliin and water extract. Aged black garlic water and ethanol extracts inhibited the $Cu^{2+}$ mediated oxidation of human LDL in a dose dependent manner at concentration of 10 and $20\;{\mu}g/ml$. Ethanol extract and water extract of aged black garlic almost completely inhibited J774 mediated LDL oxidation in electrophoretic mobility and conjugated diene. These results indicate that aged black garlic might play a protective antioxidant effects on LDL, probably affecting the structural properties for the LDL oxidation.

Antioxidative Activity of Streptomyces sp. BH-405 on Macrophage Mediated Modification of Human Low Density Lipoprotein[LDL) (Macrophage 유도 사람 Low Density Lipoprotein[LDL]의 수식에 대한 Streptomyces sp. BH-405 배양액의 항산화 활성)

  • 류병호;박종옥;김희숙;김민정
    • KSBB Journal
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    • v.15 no.2
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    • pp.156-161
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    • 2000
  • This study was designed to investigate the antioxidative activity on oxidation of human low density lipoprotein(LDL) of band 2 fractionated from culture broth of Streptomyces sp. BH-405. Antioxidative activity of band 2 obtained from fractionation of BH-405 culture purification was measured against $Cu^{2+}$ mediated human LDL oxidation by thiobarbituric acid reactive substance. $CuSO_4$ mediated oxidation of LDL was degraded at a much higher rate than native LDL. Band 2 at a concentration of 100 or 200 !lg/mL inhibited the oxidation of LDL induced by $CuSO_4$, The formation of conjugated dienes induced in the presence of 5 !1M CuS04 of the mouse macrophage and J744. The electrophoretic mobility of the LDL in addition of $200\mu\textrm{g}$ band 2 in the presence of $5\mu\textrm{m}$ $CuSO_4$ was lower than that of native LDL. LDL modified by copper mediated or cell mediated uptake was degraded by macrophage at much greater than native LDL, and band 2 was found as potential inhibitor of modification of 125I-labelled LDL by macrophage. phage.

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Screening of Antioxidative Compounds toward Human Low Density Lipoprotein (LDL) from Useful Plants (유용식물로부터 Human Low Density Lipoprotein(LDL)에 대한 항산화제의 탐색)

  • 임복규;류병호
    • The Korean Journal of Food And Nutrition
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    • v.17 no.2
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    • pp.138-146
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    • 2004
  • This study was designed to investigate antioxidative activity of substances isolated from 25 kinds of useful plants resources toward free radical and human low density lipoprotein(LDL). Methanol extracts of Oenothers odorate had the highest antioxidative activity similar with ${\alpha}$-tocopherol. Methanol extracts of Oenothers odorate was extracted again by the ethylacetate. The ethylacetate soluble acidic fraction obtained from methanol extract of Oenothers odorate showed highest activity toward human LDL. Each fraction was purified through Sepadex LH-20 chromatography by elution of chloroform-methanol mixture (90:10 v/v). Fraction, F-2 obtained from Oenothers odorate showed at highest levels of electron donating activity. Fraction, F-2 was identified as 3,4-dihydroxybenzoic acid and 3-hydroxycinnamic acid.

Discovery of Antioxidant on Human Low Density Lipoprotein (LDL) by Bacillus sp. RH-5 Isolated from Marine Origin (해양에서 분리한 Bacillus sp. RH-5에 의한 사람 Low Density Lipoprotein(LDL) 산화에 대한 항산화제의 개발)

  • 류병호;박종옥;김동석
    • Journal of Life Science
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    • v.9 no.1
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    • pp.99-105
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    • 1999
  • The aims of this studies were carried out to investigate the antioxidant activity on low density lipoprotein(LDL) using substances extracted from Bacillus sp. RH-5. The antioxidative substances produced extracellular in the culture broth by Bacillius sp. RH-S was obtained by elution of chloroform : methanol from silicagel column (80cm x100cm) chromatography. Band 4 eluted from fraction 3 by TLC was appeared at highest level of antioxidative activity using thiocyanate methed. Band 4 at a concentration of 100 or 200$\mu$g/$m\ell$ inhibited oxidation of LDL induced by the mouse transformed macrophage. According to IR. NMR or GC/MASS, the antioxidant substance was identified as 5-hydroxyindole.

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Antioxidative Effect of Silmarin Purified from Silybum Marianum on Modificaion of Human Low Density Lipoprotein (엉겅퀴에서 분리 정제한 Silymarin의 사람 Low Density Lipoprotein에 대한 항산화 효과)

  • 류병호;이백천;진성현;조경자;김동석
    • Journal of Food Hygiene and Safety
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    • v.12 no.1
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    • pp.1-8
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    • 1997
  • This study was performed to evaluate the antioxidant activity of silymarin against human low density lipoproteins(LDL) oxidation. Silymarin extracted from Silybum marianum was successively purified with solvent fractionation and followed by silica gel column chromatography. The active substances were separated by HPLC and the isolated active substances, silymarin were identified by IR, NMR, GC-MS as silymarin. Silymarin inhibited at the 5 $\mu$M Cu2+-mediated oxidation of human low density lipoprotein (LDL) in a dose dependent manner. Silymarin completely inhibited LDL oxidation at 50 $\mu\textrm{g}$/$m\ell$ concentration. These findings suggest that silymarin may protect LDL against oxidation in atherosclerotic lessions.

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Antioxidative Effect of Silybin Purified from Silybum marianum on modification of Human Low Density Lipoprotein (엉겅퀴에서 분리정제한 silybin의 Human Low Density Lipoprotein 수식에 대한 항산화 효과)

  • Ryu, Beung-Ho;Kim, Hee-Suck;Lee, Baek-Cheon;Lee, Hong-Su
    • Journal of Life Science
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    • v.8 no.4
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    • pp.353-359
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    • 1998
  • This study was initiated to antioxidant activity of silybin on oxidation of human low density lipoproteins(LDL). Siltbin was extracted from Silybum marianum by the combination of fractionation and it was futher purified by silica gel column chromatography, and isolated active substances were identified silybin by IR, NMR and GC-MS. siltbin inhibited the ozidation of human low density lipoprotein(LDL) mediated by 5$^{\mu}$m CU $^{2+}$ ion in a dose dependent manner. LDL oxidation by congugated dines formation was completely inhibited by silybin at a concentration of 5$^{\mu}$M. The results provide a possibility that silybin might protect LDL against oxidation in atherosclerotic lessions.

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Screening of Antioxidants to Human Low Density Lipoprotein (LDL) from Marine Resources (수산자원으로부터 Human Low Density Lipoprotein (LDL)에 대한 항산화제의 탐색)

  • 류병호
    • Journal of Food Hygiene and Safety
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    • v.14 no.2
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    • pp.153-159
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    • 1999
  • This study was undertaken to evaluate antioxidative activities of substances isolated from marine resources against human low density lipoprotein (LDL). Methanol-water extract(80 : 20, v/v) of Sargassum ringgoldianum had the highest antioxidant activity and the active substance was purified by silica gel column chromatography by eluting chlorform : methanol mixture (80 : 20 v/v). The active fraction was seperated to several spots on the TLC in chlorofrom : methanol (10 : 1, v/v) mixture. Antioxidative activity of band 4 of fraction 2 on TLC was highest than that of $\alpha$-tocopherol against human LDL oxidation by the method of thiobarbituric acid reactive substance (TBARS). The band 4 of fraction 2 inhibited the copper mediated oxidation of human LDL with almost completely at 1 or 2 mg/ml.

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Inhibition of Chitin Sulfate on Human Low Density Lipoprotein(LDL) Oxidation by Macrophages

  • Ryu, Beung-Ho;Yang, Seung-Taek;Moon, Yoon-Hee
    • Journal of Food Hygiene and Safety
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    • v.16 no.4
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    • pp.342-348
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    • 2001
  • Growing evidence indicates that oxidized low density lipoprotein (LDL) may promote atherogenesis. Therefore, inhibition of LDL oxidation may impede this process. The effect of chitin sulfate on the susceptibility of human low density lipoprotein (LDL) to macrophages-induced oxidation was investigated by monitoring a thiobarbituric acid reactive substance (TBARS). Chitin sulfate inhibited LDL oxidation by macrophages in a dose dependent manner, with a 50~100$\mu$M, as assessed by TBAaS assay. Chitin sulfate, at 100 $\mu$M, almost completely inhibited the macrophage-induced increase in electrophoretic mobility of LDL. Also, chitin sulfate almost completely inhibit $O_2$ ̄ at concentration of 100 $\mu$M. These observations suggest that chitin sulfate might be an effective in prevention of atherosclerosis.

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Protection Effects of Allylmercaptan, Metabolite of Garlic on Endothelial Cell Injury Induced by Oxidized Low Density Lipoprotein (산화된 low density lipoprotein (LDL)에 의해 유도된 내피세포의 손상에 대한 마늘 대사산물인 allylmercaptan의 보호 효과)

  • Yang, Seung-Taek
    • Journal of Life Science
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    • v.20 no.11
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    • pp.1595-1602
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    • 2010
  • Oxidation of low density lipoprotein (LDL) has been recognized as an important role in the initiation and progression of atherosclerosis. In this study, effects of allylmercaptan, a major metabolite compound of garlic, was studied on endothelial cell injury induced by oxidized low density lipoprotein (ox-LDL). The antioxidative activity of allylmercaptan was investigated by monitoring a thiobarbituric acid substance (TBARS). Allylmercaptan inhibited LDL oxidation induced by $Cu^{2+}$ at concentrations of 0.1, 1 and 10 mM in a dose dependent manner. Lactate dehydrogenase (LDH) release, as an index of cell injury, and intracellular glutathione levels were determined. Pulmonary artery endothelial cells were preincubated with allylmercaptan at $37^{\circ}C$ and 5% $CO_2$ for 24 hr, washed, and then exposed to 0.1 mg/ml oxidized LDL for 24 hr. Preincubation of endothelial cells with allylmercaptan significantly prevented the LDH release and depletion of GSH. Peroxides were measured directly in 24 well plates using a fluorometric assay. Allylmercaptan inhibited release of peroxides induced by ox-LDL in pulmonary artery endothelial cells. In a free system, allylmercaptan was shown to scavenge hydrogen peroxide. The data indicate that allylmercaptan can protect pulmonary artery endothelial cells from injury caused by oxidized LDL, and suggest that allylmercaptan may be useful for the prevention of atherosclerosis.