• 한국토양비료학회지
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• 제40권4호
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• pp.229-233
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• 2007

탈수소효소(Dehydrogenase)생성균과 탈수소효소활성의 논토양 건전성평가 생물지표로서의 기능을 평가하고자, 동일 비료 장기연용 논토양의 무처리구, 질소시용구 (N), 화학비료시용구 (NPK), 그리고 퇴비와 화학비료 혼합시용구 (CNPK)를 대상으로 담수전에 시료를 채취하여 탈수소효소를 분비하는 세균과 Bacillus 그리고 탈수소효소활성을 조사하였다. 논토양의 탈수소효소활성은 유기물함량이 비교적 높고 유효인산 많은 화학비료시용구(NPK) 및 퇴비와 화학비료 혼합시용구(CNPK)에서 높은 경향이었다. 토양의 탈수소효소생성균 밀도와 탈수소효소활성간에는 유의성이 높은 정의 상관관계를 보였다.

• 한국토양비료학회지
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• 제46권6호
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• pp.474-481
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• 2013

Understanding the microbial community structure of agricultural soils is important for better soil management in order to improve soil quality. Phospholipid fatty acid analysis has been popularly used in determining the microbial community structure in different ecosystems. The microbial community structure of paddy soil under long-term fertilizer treatments was investigated after 45 years using PLFA analysis. Treatments were control (no fertilization, Con), compost (COM), NPK, NPK+compost (NPKC), PK, NK, and NP. Soil chemical properties were mainly affected by the addition of compost and inorganic P fertilizer. Total nitrogen and organic matter contents were significantly higher in treatments with compost while available $P_2O_5$ and exchangeable calcium were significantly higher in treatments with added inorganic P fertilizer. It was found that microbial communities were responsive to the different fertilizer treatments. PLFA results showed that the soils were dominated by gram-negative bacteria, followed by the actinomycetes, then gram-positive bacteria, and fungi. Principal component analysis of the soil chemical properties and PLFA composition proved to be a more reliable tool because it was more responsive to the changes in soil chemical properties.

• Applied Biological Chemistry
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• 제49권3호
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• pp.175-179
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• 2006

동일비료를 장기연용한 토양에서 벼를 단작으로 재배하여 비료의 시용에 따른 벼 품종간 근권 세균 및 방선균의 밀도와 우점균을 조사하였다. 비료는 3요소(NPK)를 기본으로 하여 무질소(PK), 무인산(NK) 무칼리(NP) 및 NPK+퇴비를 처리하였다. 벼의 근권 미생물 밀도는 품종에 따라서는 큰 차이를 보이지 않았고 시용비료에 의해 약간의 차이를 보였는데 세균은 감소하고 방선균은 증가하는 경향을 나타내었다. 근권 미생물의 분포상에는 유기물의 영향이 뚜렷하여 방선균의 경우 NPK+퇴비구 처리구에서 가장 높은 균 밀도를 나타냈다. 배양가능한 호기성 세균에서는 Bacillus속의 B. megaterium, B. mycoides, B. licheniformis, B. subtilis 등이, 방선균에서는 Streptomyces속의 S. spororaveus, S. canus, S. tauricus, S. galbus 등과 Micromonospora속이 우점하여 분포하였다.

• 한국토양비료학회지
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• 제31권4호
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• pp.330-333
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• 1998

본 시험은 석회 및 규산의 시용이 콩의 수량 및 종실에 미치는 영향을 조사하기 위하여 수행하였다. 콩의 수량은 NPK+석회 $1.5Mg\;ha^{-1}$ 처리가 가장 높았다. 탄수화물 함량은 무비에서 가장 높았고 단백질 함량은 NPK+석회 $1.5Mg\;ha^{-1}$ 및 NPK+규산 $1.5Mg\;ha^{-1}$ 처리구에서 높았으며 지방 및 회분함량은 NPK+규산 $1.5Mg\;ha^{-1}$ 처리구에서 높았다. 무기성분 함량은 처리간에 차이가 없었다. $P_2O_5$/T-N과 탄수화물 사이에는 $-0.99^{**}$, $P_2O_5$/T-N과 지방 사이에는 $-0.94^{**}$의 상관이 있었다. 그리고 Mn과 탄수화물사이에는 $0.95^*$의 상관이 있었다. 석회의 시용은 콩의 수량에, 규산의 시용은 단백질 함량증가에 효과적이었다.

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1292-1298
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• 2022

Soil autotrophic bacterial communities play a significant role in the soil carbon (C) cycle in paddy fields, but little is known about how rhizosphere soil microorganisms respond to different long-term (35 years) fertilization practices under double rice cropping ecosystems in southern China. Here, we investigated the variation characteristics of rhizosphere soil RubisCO gene cbbL in the double rice ecosystems of in southern China where such fertilization practices are used. For this experiment we set up the following fertilizer regime: without any fertilizer input as a control (CK), inorganic fertilizer (MF), straw returning (RF), and organic and inorganic fertilizer (OM). We found that abundances of cbbL, 16S rRNA genes and RubisCO activity in rhizosphere soil with OM, RF and MF treatments were significantly higher than that of CK treatment. The abundances of cbbL and 16S rRNA genes in rhizosphere soil with OM treatment were 5.46 and 3.64 times higher than that of CK treatment, respectively. Rhizosphere soil RubisCO activity with OM and RF treatments increased by 50.56 and 45.22%, compared to CK treatment. Shannon and Chao1 indices for rhizosphere soil cbbL libraries with RF and OM treatments increased by 44.28, 28.56, 29.60, and 23.13% compared to CK treatment. Rhizosphere soil cbbL sequences with MF, RF and OM treatments mainly belonged to Variovorax paradoxus, uncultured proteobacterium, Ralstonia pickettii, Thermononospora curvata, and Azoarcus sp.KH33C. Meanwhile, cbbL-carrying bacterial composition was obviously influenced by soil bulk density, rhizosphere soil dissolved organic C, soil organic C, and microbial biomass C contents. Fertilizer practices were the principal factor influencing rhizosphere soil cbbL-carrying bacterial communities. These results showed that rhizosphere soil autotrophic bacterial communities were significantly changed under conditions of different long-term fertilization practices Therefore, increasing rhizosphere soil autotrophic bacteria community with crop residue and organic manure practices was found to be beneficial for management of double rice ecosystems in southern China.

• 한국토양비료학회지
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• 제29권2호
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• pp.150-157
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• 1996

동일비료(同一肥料) 및 퇴비(堆肥), 석회(石灰), 규산질비료(珪酸質肥料) 등(等)의 장기연용(長期連用)이 논 토양(土壤) 및 현미중(玄米中) 중금속함량(重金屬含量)에 미치는 토양개량효과(土壤改良效果)를 밝힐 목적(目的)에서 조사(調査) 분석(分析)한 결과(結果)는 다음과 같다. 1. 황산(黃酸)암모늄, 요소(尿素), 3요소(要素) 등(等) 비료(肥料)의 장기연용(長期連用)으로 토양중(土壤中) Cu, Zn(0.1N-HCl 침출)함량(含量)은 증가(增加)한 반면 현미중(玄米中) Cu, Zn함량(含量)은 감소(減少)하는 경향(傾向)이었고 Cd, Pb함량(含量)은 토양(土壤) 및 현미(玄米)에서 일정한 경향을 보이지 않았다. 2. 석회(石灰) 및 규산질비료(珪酸質肥料)의 장기연용(長期連用)으로 토양중(土壤中) Cu, Zn함량(含量)은 증가(增加)한 반면 현미중(玄米中) Cu, Zn함량(含量)은 감소(減少)하는 경향(傾向)이였고 Cd, Pb함량(含量)은 토양(土壤) 및 현미(玄米)에서 일정한 경향을 보이지 않았다. 3. 퇴비장기(堆肥長期) 연용(連用)으로 토양중(土壤中) Cu 및 Zu함량(含量)은 증가(增加)하였으나 Cd, Pb함량(含量)은 토양(土壤) 및 현미(玄米)에서 일정한 경향이 없었다.

• Journal of Microbiology and Biotechnology
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• 제29권2호
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• pp.292-296
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• 2019

Soils amended for long-term with high levels of compost demonstrated greater abundance of bacterial members of the phylum Bacteroidetes whereas a decreasing trend in the relative abundance of phylum Acidobacteria was noted with increasing levels of compost. Metabolic profiles predicted by PICRUSt demonstrated differences in functional responses of the bacterial community according to the treatments. Soils amended with lower compost levels were characterized by abundance of genes encoding enzymes contributing to membrane transport and cell growth whereas genes encoding enzymes related to protein folding and transcription were enriched in soils amended with high levels of compost. Thus, the results of the current study provide extensive evidence of the influence of different compost levels on bacterial diversity and community structure in paddy soils.

• 한국토양비료학회지
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• 제49권2호
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• pp.194-199
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• 2016

In an experiment conducted at the research field of the National Institute of Agricultural Science, we investigated the effects of mineral fertilizer and rice straw compost on exchangeable potassium and K balances, and rice grain yield under a rice single system. The treatments were no fertilization (No fert.), inorganic fertilization (N), inorganic fertilizer (N, P, K) plus rice straw compost at rates of 7.5, 15.0, 22.5, and $30.0ton\;ha^{-1}$ (NPKC7.5, NPKC15.0, NPKC22.5, and NPKC30.0, respectively). The inorganic fertilizers(N, P, K) were added with standard fertilizer application rate in which nitrogen (N), phosphate ($P_2O_5$), and potassium ($K_2O$) were applied with $75{\sim}150kg\;ha^{-1}$, $70{\sim}86kg\;ha^{-1}$, $75{\sim}86kg\;ha^{-1}$, respectively. Exchangeable potassium for NPKC15.0 NPKC22.5, and NPK30.0 treatments was higher by $0.05{\sim}0.19cmol_c\;kg^{-1}$ than that of NPKC7.5 treatment. Increasing levels of rice straw compost resulted in an increase in the K balance from - $19.9kg\;ha^{-1}yr^{-1}$ (No fert.) to $41.9kg\;ha^{-1}yr^{-1}$ at NPKC22.5 treatment and $62.9kg\;ha^{-1}$ at NPKC30.0 treatment. Continuous application of rice straw compost with NPK fertilizers affected significantly the rice grain yields. The result of the study imply that the application of more than $22.5ton\;ha^{-1}$ of rice straw compost with NPK fertilizers are recommended as the best fertilization practice for enhancement of crop production and K supplying power of soil in the continuous rice cropping system.

• 한국토양비료학회지
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• 제47권6호
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• pp.451-456
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• 2014

Organic amendment practices can influence diversity and activities of soil microorganisms. There is a need to investigate this impact compared with other types of materials. This study was carried out to evaluate the long term effects of chemical and organic fertilizer on soil microbial community in upland field. During the last 11 years green manure, rice straw compost, rapeseed cake, pig mature compost, NPK, and NPK + pig mature compost were treated in upland soil. Organic fertilizer treatment found with high bacterial colony forming units (CFUs) as compared to chemical and without fertilizer treatment. There was no significant difference in the actinomycetes and fungal population. The average well color development (AWCD) value was the highest in green manure and, the lowest in without fertilizer treatment. Analyses based on the denaturing gradient gel electrophoresis (DGGE) profile showed that rice straw compost and pig mature compost had a similar banding pattern while rapeseed cake, NPK, NPK + pig mature compost and without fertilizer treatment were clustered in another cluster and clearly distinguished from green manure treatment. Bacterial diversity can be highly increased by the application of organic fertilizer while chemical fertilizer had less impact. It can be concluded that green manure had a beneficial impact on soil microbial flora, while, the use of chemical fertilizer could affect the soil bacterial communities adversely.

• 한국토양비료학회지
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• 제45권6호
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• pp.1100-1107
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• 2012

화학비료의 장기 시용이 벼 내생 세균 군집에 미치는 영향을 조사하기 위해서 국립농업과학원의 장기 비료 연용 포장에서 재배한 벼 뿌리의 내생균의 군집을 파이로시퀀싱 기법으로 분석하였다. 3요소구 (APK)와 무비구 (NF) 시료에서 직접 DNA를 추출하여 세균에 특이적인 barcode PCR을 수행한 후 454 파이로시퀀싱을 하였다. 두 시료 (3요소구, 무비구)에서 1,900개의 염기서열을 얻었으며, 각각 177개와 72개의 OTU로 분류하였다. 두 시료는 22개의 OTU를 공유하였으며, 이들 OTU는 두 시료에서 모두 우점하였다. 특히 Pseudomonas속에 속하는 OTU의 비율이 매우 높았다. 문 (phylum) 수준에서 우점하는 내생균은 두 시료 모두 Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Actinobacteria 등 이었다. 처리구별로 계산한 다양성 지수는 3요소구 시료에서 더 높았다. 본 연구를 통해 장기간 비료 시용은 식물체내 존재하는 내생균 군집 구조에 영향을 주며, 벼 뿌리의 내생 세균의 군집 다양성을 증가시키는 것을 알 수 있었다.