• 생약학회지
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• 제49권2호
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• pp.103-107
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• 2018

Two phenylpropanoids, three caffeoylquinic acid deravatives and an iridoid were isolated from the stem of Lonicera macckii. On the basis of physico-chemical data, these compounds were identified as caffeic acid methyl ester(1), 5-caffeoylquinic acid n-butyl ester(2), methyl 3,4-dicaffeoyl quinate(3), 3,5-dicaffeoyl quinic acid n-butyl ester(4), loganin (5) and caffeic acid(6). All compounds were isolated for the first time from the stem of this plant.

• 대한한의학회지
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• 제25권3호
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• pp.45-54
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• 2004

Objectives : This study was aimed to evaluate marker substances in Gami-Honghwa-Tang (KH-19) by high performance liquid chromatography-photodiode array detector (HPLC-DAD). Gami-Honghwa-Tang is composed of nine crude herbs, Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Cnidii Rhizoma, Paeoniae Radix, Corni Fructus, Moutan Cortex Radicis, Lycii Fructus, Carthami Flos and Glycyrrhizae Radix. Methods : The separation was performed on an Aquasil C18 (4.6×250mm) column by gradient elution with 0.05% TFA in H2O - 0.05% TFA in acetonitrile (0 min 100:0, 20 min 90:10, 40 min 70:30, 60 min 50:50, 80 min 0:100, 90 min 100:0) as the mobile phase at a flow-rate of 1.0 ml/min with detection at 190-800nm. Also we examined the contents for bacteria, pesticide residue and harmful heavy metals. Results : HPLC-DAD was employed to determine the quantities and the qualities of several marker substances such as 5­hydroxymethyl-2-furaldehyde (5-HMF), paeonol, loganin, paeoniflorin, glycyrrhizin, and decursin in the KH-19. There were no bacterial contents, pesticide residues, or harmful heavy metals. Conclusions : We suggest these results could be a useful evidence for quality control of KH-19. This method permits fingerprints of selected individual marker substances from herbal prescriptions without derivatization, multiple purification steps, or lengthy separation times.

• 생약학회지
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• 제43권2호
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• pp.137-146
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• 2012

Dipsaci Radix (Dipsacaceae) has been used as a tonic, an analgesic, anti-inflammatory and anti-complement agents in traditional herbal medicine for the therapy of low back pain, knee pain, rheumatic arthritis, traumatic hematoma, and bone fractures. A high-performance liquid chromatography-electrospray ionization-mass spectrometric method (HPLC-ESI-MS) was developed for the simultaneous quantitation method of the five compounds from the herbal drug: asperosaponin VI and asperosaponin XII (terpene glycosides), sweroside, loganin and dipsacus A(iridoid glycosides). HPLC separation of the analytes was achieved on a C18 column ($150{\times}2.0$ mm i.d., 5 ${\mu}m$) using the aqueous methanol containing 5 mM ammonium acetate with gradient flow of the mobile phase. Detection of the analytes was performed by positive ion electrospray ionization, and selected ion monitoring was used for data acquisition using m/z corresponding molecular adduct ion, $[M+NH_4]^+$ and $[M+H]^+$. Calibration graphs showed good linearity ($r^2$=0.9997) over the wide range of the analytes; intra- and inter-day precisions (RSD, %) were within 9.1% and the accuracy between 94.0-111.0%. Recoveries of the analytes through the assay procedure were in the range of 93.7-110.8%. Analytical results of the herbal drugs of Dipsaci Radix (17 samples) show wide distribution of the five marker compounds and clear difference of the species from Phlomidis Radix (4 samples). The developed method would provide a practical guide for the quality control of the herbal drug.

• 약학회지
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• 제54권3호
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• pp.157-163
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• 2010

A high-performance liquid chromatography (HPLC) with DAD detector and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of coniferin (1), loganic acid (2), demethylsecologanol (3), sweroside (4) and loganin (5) from caulis Lonicera joponica. The optimal chromatographic conditions were obtained on an ODS column ($5{\mu}m$, $4.6{\times}150mm$) with the column temperature $35^{\circ}C$. The mobile phase was composed of (A) water with 0.1% formic acid and (B) methanol with 0.1% formic acid using a gradient elution, the flow rate was 0.3 ml/min. Detection wavelength was set at 254 nm. All calibration curves showed good linear regression ($r^2$>0.998) within test ranges. The developed method provided satisfactory precision and accuracy with overall intra-day and interday variations of 0.16~3.28% and 0.14~1.99%, respectively, and the overall recoveries of 99.39~105.89% for the five compounds analyzed. The verified method was successfully applied to quantitative determination of the two types (phenolic compounds and iridoids) of bioactive compounds in 24 commercial caulis L. japonica samples from different markets in Korea and China. The analytical results demonstrated that the contents of the five analytes vary significantly with sources.

• 한국식품영양과학회지
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• 제46권9호
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• pp.1061-1070
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• 2017

본 연구에서는 가압증숙 산수유의 이화학적 품질특성 변화를 모니터링 하였고, 폐 표피세포 보호 효과에 대해 알아보기 위하여 인간유래 L132 세포주를 이용하여 과산화수소로 유도된 산화 스트레스 모델에서 가압증숙 산수유 추출물의 세포 사멸 억제 효과를 확인하였다. 색도에서 명도를 나타내는 L값은 무처리 산수유 분말에서 42.75로 가장 높게 나타났으며, 대조군에 비해 가압증숙 시간이 증가할수록 L값은 유의적으로 감소하였다. 적색도(a값) 및 황색도(b값) 역시 이와 유사한 경향을 나타내었는데, 외형을 살펴보면 2시간 이상 가압증숙 시 색이 검게 변하고 광택이 없어지는 경향을 나타내었다. 2시간 동안 가압증숙 시 총 당, 환원당 및 총 페놀 함량변화를 측정한 결과, 각각 468.53 mg/g, 385.55 mg/g 및 37.32 mg/g으로 나타났다. 유리당 조성은 fructose, glucose 및 sucrose 순이었으며, 2시간 동안 가압증숙 시 각각 207.72 mg/g, 219.40 mg/g 및 4.31 mg/g으로 낮은 함량을 나타내었다. 페놀화합물인 gallic acid 및 furan 화합물인 5-HMF는 대조군에 비해 가압증숙 시간이 증가할수록 함량이 유의적으로 높아짐을 확인하였으며(P<0.05), iridoid 배당체 조성은 morroniside, loganin 및 loganic acid 순으로 함유하고 있었고 2시간 동안 가압증숙 시 다소 낮아지는 경향을 나타내었다. 과산화수소에 의해 유도된 산화적 세포 사멸에 대한 추출물의 보호 효과를 확인하기 위해 가압증숙 산수유 추출물의 L132 세포 독성을 확인한 결과 $1,000{\mu}g/mL$ 농도까지 유의적으로 세포 사멸이 나타나지 않아 세포독성이 없음을 확인할 수 있었다. L132 세포 사멸에 대한 보호 효과는 모든 시료에서 1 mM $H_2O_2$를 첨가한 군과 비교하여 활성이 유의적으로 증가하였으며, 특히 2시간 동안 가압증숙한 산수유 추출물을 $1,000{\mu}g/mL$ 농도로 첨가하였을 때 102.82%로 활성이 가장 크게 증가하여 과산화수소에 의해 유도된 산화적 세포 사멸에 대한 높은 세포 보호 효과를 나타냄을 확인하였다.

• Journal of Ginseng Research
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• 제31권4호
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• pp.196-202
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• 2007

본 실험은 홍삼, 사상자, 산수유를 이용하여 주름 예방 효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 개별추출물과 이들의 혼합물 WM1, WM3 (KTNG0345)에 대하여 사람의 섬유아세포를 이용한 세포증식, 콜라겐 생합성 정도, MMP-1 효소활성, 항산화 활성을 조사하였다. 사용된 시료는 $1{\sim}10\;{\mu}g/ml$의 농도로 첨가하여 세포를 배양했을 때 $83{\sim}100%$의 세포증식율을 나타내어 세포 독성을 나타내지 않았으며, 콜라겐 생합성 정도는 대조군이 474.8 ng/ml이었고 retinoic acid는 568.3 ng/ml이었으며, 5 ug/ml의 농도를 기준으로 할 때 홍삼은 533.9ng/ml, 사상자는 539.3 ng/ml, 산수유는 514.1 ng/ml이었다. 홍삼과 생약을 68:232의 비율로 혼합한 WM1은 474.6 ng/ml이었고 홍삼과 생약을 136:164의 비율로 혼합한 WM3는 561.4 ng/ml로 증가하였다. MMP-1활성은 10 ug/ml의 농도에서 홍삼(ER)은 31.9 ng/ml, 사상자(WT)는 32.85 ng/ml, 산수유(WC)는 32.0 ng/ml, WM1은 31.3 ng/ml이었고 WM3(KTNG0345)는 28.85 ng/ml으로 대조군의 88% 수준으로 감소하였다. SOD활성에서는 1000 ug/ml의 농도로 처리하였을 때 65-97% 항산화활성을 나타내었다. 이들의 결과를 종합해 보면 홍삼과 사상자, 산수유 및 이들 혼합물은 human dermal fibroblas에 대하여 세포 독성을 나타내지 않으면서 콜라겐 생합성을 촉진하고 MMP-1의 활성을 억제하였으며 홍삼과 생약 엑기스를 136:164의 비율로 혼합했을 때 그 효과가 증진되었다. WM3 (KTNG0345)는 지표성분으로서 ginsenoside-$Rb_1$, torilin, loganin을 각각 10.85 mg/g, 0.128 mg/g, 3.92 mg/g 함유하였다.

• 생명과학회지
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• 제31권3호
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• pp.287-297
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• 2021

산수유 나무는 그 열매인 산수유와 함께 동아시아의 많은 국가에서 식용 및 약용으로 오랫동안 널리 사용되고 있다. 선행 연구들에 의하면 산수유 나무 추출물 및 구성 성분은 다양한 약리학적 잠재력을 가지고 있음에도 불구하고 부위별 추출물에 대한 체계적인 성분 분석은 아직 충분하지 않은 실정이다. 본 연구에서는 산수유 나무의 각 부위(청산수유, 적산수유, 씨앗, 줄기, 잎, 건피)의 유효 성분 함량을 분석하여 항산화 활성을 검증하였다. 본 연구의 결과에 따르면 조사된 3가지 지표 성분 중 morroniside은 청산수유와 적산수유에 가장 많이 함유하고 있었으며, loganin은 청산수유와 씨앗에 많았다. 잎, 적산수유, 건피의 함량은 비슷한 수준이었다. Cornin은 잎에 가장 많았으며, 청산수유, 적산수유 건피 순이었다. 또한 총 폴리페놀 함량은 적산수유, 청산수유, 잎 순으로 높았으며, 총 플라보노이드 함량은 잎이 가장 높았다. 그리고, DPPH radical 소거능은 잎, 씨앗, 적산수유 추출물 순으로 높은 소거 활성을 나타냈다. 따라서 항산화 활성이 가장 높은 잎 추출물의 항염증 효능을 조사하였으며, 이를 위하여 LPS로 자극된 RAW 264.7 대식세포에서 NO와 같은 염증성 매개 인자와 IL-1β와 IL-6를 포함한 염증성 사이토카인의 생성에 미치는 잎 추출물의 영향을 평가하였다. 본 연구의 결과에 의하면 세포독성이 없는 조건에서 잎 추출물은 LPS에 의하여 증가된 iNOS의 발현과 NO의 생성을 유의적으로 억제하였다. 또한 잎 추출물은 LPS에 의한 IL-1β 및 IL-6의 생성 및 발현을 현저하게 감소시켰다. 따라서 산수유 나무의 잎 추출물은 항산화제와 항염증제의 개발을 위한 천연 재료로서 잠재력이 있음을 시사하며, 관련 기전 연구가 추가로 진행되어야 할 것이다.

• 동의생리병리학회지
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• 제23권1호
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• pp.15-26
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• 2009

The purpose of this report was to provide the information about activity and safety of Yukmijihwang-tang by analyzing domestic/international papers and theses about Yukmijihwang-tang. Domestic/international papers and theses related to Yukmijihwang-tang were reviewed and analyzed. These papers were then classified by year, experimental method, and activity subject. The following results were obtained in this study. The study of Yukmijihwang-tang started from 1978 and was rapidly increased after 1990s. The study of Yukmijihwang-tang was continuously increased now and was mainly forcused on experimental model rather than clinical study. The paper of SCI journal or non-SCI journal was 27 volume and the other domestic paper was 64 volume. The total papers of Yukmijihwang-tang, 91 volume was analysed in this study. Allatoin, gallic acid, loganin, morroniside, paeoniflorin, paenol, urosolic acid were determined in Yukmijihwang-tang by using HPLC and HPLC-MS-MS. In classified Yukmijihwang-tang paper by experimental method and animal, more than a half study was in vivo experiment used rat. Furthermore clinical experiments were performed variously. As these studies were classified by subject, papers related to renal function were most abundant by 16 papers. Besides there were several papers related to cognitive vitality, anti-diabetic effect, immuno-regulation, reproductive activity, anti-oxidant effect, liver function, anti-cancer and blood pressure depress. According to basic research and clinic research data, it is supported that Yukmijihwang-tang was useful prescription in renal function, cognitive vitality, anti-diabetic effect and reproductive activity. Many study of basic and clinic research were performed and reported variously on Yukmijihwang-tang in domestic/international journal. According to basic research and clinic research data, it is supported that Yukmijihwang-tang was useful prescription in renal function, cognitive vitality, anti-diabetic effect and reproductive activity. However, studies on efficacy and mechanism of Yukmijihwang-tang should be conducted at the molecular biology level and studies on safety of Yukmijihwang-tang need to be completed at the clinical level.

• 대한본초학회지
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• 제34권6호
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• pp.57-62
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• 2019

Objective : Gastritis is a major complication of gastrointestinal disease. Lonicera japonica is used in folk medicine to treat different diseases such as exopathogenic wind-heat, epidemic febrile diseases, sores, carbuncles and some infectious diseases. Therefore, this study examined the effects of Lonicera japonica water extract (LJE) on HCl/ethano-linduced acute gastric ulceration and anti-oxidants properties. Methods : LC-ESI-IT-TOF MS was employed for rapid identification of major compound from LJE. The antioxidant activities were evaluated through total polyphenol and flavonoid contents and radical scavenging assays and superoxide dismutase (SOD)-like activity. SD rats were randomly divided into five different groups including the normal group, ulcer group, positive group (20 kg/mg of omeprazole, ip), and experimental groups (100 kg/mg and 500 kg/mg of LJE, ip). Results : 4,5-Dicaffeoyl quinic acid, loganic acid, secologanic acid, sweroside, loganin, vogeloside were identified based on the detection of the molecular ion with those of literature data. The LJE was possessed free radical scavenging activities such as DPPH (IC₅₀=189.7 ㎍/㎖), ABTS (IC₅₀=164.5 ㎍/㎖), and SOD-like activity (IC₅₀=405.02 ㎍/㎖). Macroscopic and histological analyses showed LJE treated group were significantly reduced to an extent that it allowed leukocytes penetration of the gastric walls compared with the ulcer group. In addition, an ulcer inhibition rate and prostaglandin E₂ levels were increased in rats treated with LJE. Conclusion : The present study has demonstrated the antioxidantive and gastroprotective effect of LJE, these findings suggested that LJE has the potential for use in treatment of gastric disorders.