• Journal of Pharmaceutical Investigation
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• v.31 no.4
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• pp.225-232
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• 2001

The present study was carried out to observe the effect of liposome dispersed gel formulation (Lipogel) on topical delivery of ascorbyl palmitate (AsP). Neutral and negatively charged MLV liposomes containing AsP were prepared with dimyristoylphosphadtidylcholine (DMPC) and dicetyl phosphate (DCP), and dispersed to poloxamer gel matrix. In the hydrolysis study in rat's skin homogenates, AsP hydrolyzed to ascorbic acid (AsA) according to the first-order kinetics with the rate constant of $2.46{\times}10^{-2}\;min^{-1}$. In the passive skin penetration study using Franz diffusion cell, lipogel systems exhibited the greater values in the flux $(J_s)$ and the amount penetrated $(Q_p)$ compared to control hydrogels containing diethyleneglycol monoethyl ether $(Transcutol^{\circledR})$ as a solubilizing agent and a penetration enhancer for AsP. The total amount penetrated $(Q_{Total})$, which is expressed as a summation of $Q_P\;and\;Q_L$, for lipogel system was about 1.4 times higher in average than that of control hydrogel. However the amount localized in the skin $(Q_L)$ was similar in both formulations. As a result, lipogel system enhanced the skin penetration of AsP, possibly due to the increase in local concentration of AsP by preferential adsorption of liposome to the skin and the enhancing effect of phospholipid in liposome composition. Moreover it was expected that the penetrated AsP would generate AsA during skin penetration by the skin esterase. In conclusion, lipogel formulation was considered as a good candidate for topical delivery of AsP.

• Korean Journal of Bronchoesophagology
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• v.17 no.1
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• pp.35-39
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• 2011

Objectives The high sensitivity of ultrasound and thyroglobulin determination for follow-up of differentiated thyroid cancer allows early detection of nonpalpable recurrences. Intraoperative localization of these small foci in previously dissected necks is a surgical challenge. We assessed the safety and effectiveness of ultrasound-guided tattooing (US-tattoo) with a charcoal suspension for localizing nonpalpable cervical recurrences after thyroidectomy for thyroid cancer. Subjects and Methods Between March 2009 and December 2010, we retrospectively reviewed 19 patients who underwent US-tattoo with injection of a charcoal suspension for recurrent thyroid papillary cancer on central neck compartment after thyroidectomy. All patients underwent the surgical dissection after US-tattoo. The complications and effectiveness of US-tattoo were evaluated. Results The technical success rate of US-tattoo for suspicious lesions was 100%. There was no complication with regard to US-tattoo. During surgery, all but one tattooed lesions were detected by surgeons. On final pathologic reports, all recurrence lesions but two cases were successfully removed. Conclusion Preoperative US-tattoo is a safe and effective method for successful reoperation of central neck compartment recurrences after thyroidectomy.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.20 no.4
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• pp.501-507
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• 2007

In the first part of this study, the moving least squares finite difference method for solving solid mechanics problems was formulated. This second part verified the accuracy, robustness and effectiveness of the developed method through several numerical examples. It was shown that the method gives excellent convergence rate for elasticity problem. The solution process of elastic crack problems showed the easiness in discontinuity modeling and demonstrates the accuracy and efficiency in finding singular stress solution based on adaptive node distribution. The applicability to the engineering problem with abrupt change in displacement and stresses gradient fields is verified through a localization band problem. The developed method is expected to be extended to the various special engineering problems.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.45-45
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• 2003

We isolated a novel ankyrin-repeat containing protein, rSIAP (rSlo Interacting Ankyrin-repeat Protein), as an interacting protein to the cytosolic domain of the alpha-subunit of rat large-conductance Ca$\^$2+/-activated K$\^$+/ channel (rSlo) by yeast two-hybrid screening. Affinity pull-down assay showed the direct and specific interaction between rSIAP and rSlo domain. The channel-binding proteins can be classified into several categories according to their functional effects on the channel proteins, i.e. signaling adaptors, scaffolding net, molecular tuners, molecular chaperones, etc. To obtain initial clues on its functional roles, we investigated the cellular localization of rSIAP using immunofluorescent staining. The results showed the possible co-localization of rSlo and rSIAP protein near the plasma membrane, when co-expressed in CHO cells. We then investigated the functional effects of rSIAP on the rSlo channel using electrophysiological means. The co-expression of rSIAP accelerated the activation of rSlo channel. These effects were initiated at the micromolar [Ca$\^$2+/]$\_$i/ and gradually increased as [Ca$\^$2+/]$\_$i/ raised. Interestingly, rSIAP decreased the inactivation kinetics of rSlo channel at micromolar [Ca$\^$2+/]$\_$i/, while the rate was accelerated at sub-micromolar [Ca$\^$2+/]$\_$i/. These results suggest that rSIAP may modulate the activity of native BK$\_$Ca/ channel by altering its gating kinetics depending on [Ca$\^$2+/]$\_$i/. To localize critical regions involved in protein-protein interaction between rSlo and rSIAP, a series of sub-domain constructs were generated. We are currently investigating sub-domain interaction using both of yeast two-hybrid method and in vitro binding assay.

• BMB Reports
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• v.42 no.12
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• pp.817-822
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• 2009

Type-1 phosphatase (PP-1) was assessed in foot muscle (FM) and hepatopancreas (HP) of estivating (EST) Otala lactea. Snail PP-1 displayed several conserved traits, including sensitivity to inhibitors, substrate affinity, and reduction in size to a 39 kDa catalytic subunit (PP-1c). During EST, PP-1 activity in FM and HP crude extracts was reduced, though kinetics and protein levels of purified PP-1c isoforms were not altered. PP-1c protein levels increased and decreased in nuclear and glycogen-associated fractions, respectively, during EST. Gel filtration determined that a 257 kDa low $K_m$ PP-1$\alpha$ complex decreased during estivation whereas a 76 kDa high $K_m$ complex increased in EST. Western blotting confirmed that the 76 kDa protein consisted of PP-1$\alpha$ and nuclear inhibitor of PP-1 (NIPP-1). A suppression of PP-1 activity factors in the overall metabolic rate depression in estivating snails and the mechanism is mediated through altered cellular localization and interaction with binding partners.

• Journal of Applied Reliability
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• v.16 no.3
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• pp.238-244
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• 2016

Purpose: Domestic electric railway Regenerative Energy Storage System seriously affects the maintenance cost of the total operating expenses of nearly 60% of the total LCC (Life Cycle Cost) due to high dependence on foreign Leading company. Therefore by developing the system, it is important to lower the maintenance cost in the domestic supply. This study about the capacitor Reliability test and the purpose of this study is development electric railway Regenerative Energy Storage System. Methods: In case of, having a close relation between the temperature and the reaction rate, Accelerated Model was known that according to Arrhenius' law of chemical activity. If you apply this formula in using allowable temperature range of the capacitor can induce the Arrhenius empirical formula used in much Manufacture Fields. We evaluate the capacitors Leading company through the Arrhenius model. in order to providing a base for the localization of Ultra Capacitor. Conclusion: In this paper, we conducted a reliability test. And it was performed by the accelerated life test and Cycle Test with temperature and C-rate. and then MTBF and B10 life are estimated by analyzing the accelerated life test result. This is thought to need detailed study applying complex stress than about whether it matches the actual behavior in electric railway.

• Radiation Oncology Journal
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• v.2 no.1
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• pp.129-137
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• 1984

In brachytherapy, it is important to determine the positions of the radiation sources which are inserted into a patient and to estimate the dose resulting from the treatment. Calculation of the dose distribution throughout an implant is so laborious that it is rarely done by manual methods except for model cases. It is possible to calculate isodose distributions and tumor doses for individual patients by the use of a microcomputer. In this program, the dose rate and dose distributions are calculated by numerical integration of point source and the localization of radiation sources are obtained from two radiographs at right angles taken by a simulator developed for the treatment planning. By using microcomputer for brachytherapy, we obtained the result as following 1. Dose calculation and irradiation time for tumor could be calculated under one or five seconds after input data. 2. It was same value under$\pm2\%$ error between dose calculation by computer program and measurement dose. 3. It took about five minutes to reconstruct completely dose distribution for intracavitary irradiation. 4. Calculating by computer made remarkly reduction of dose errors compared with Quimby's calculation in interstitial radiation implantation. 5. It could calculate the biological isoffect dose for high and low dose rate activities.

• Journal of IKEEE
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• v.22 no.3
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• pp.558-564
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• 2018

In distributed estimation where each node can collect only partial information on the parameter of interest without communication between nodes and quantize it before transmission to a fusion node which conducts estimation of the parameter, we consider a novel quantization technique employed at local nodes. It should be noted that the performance can be greatly improved if each node can transmit its measurement to one designated node (namely, head node) which can quantize its estimate using the total rate available in the system. For this case, the best strategy at the head node would be simply to partition the parameter space using the generalized Lloyd algorithm, producing the global codewords, one of which is closest to the estimate is transmitted to a fusion node. In this paper, we propose an iterative design algorithm that seeks to efficiently assign the codewords into each of quantization partitions at nodes so as to achieve the performance close to that of the system with the head node. We show through extensive experiments that the proposed algorithm offers a performance improvement in rate-distortion perspective as compared with previous novel techniques.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.5615-5620
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• 2013

Nucleophosmin (NPM1) is a protein of highly conserved nature which works as a molecular chaperone and is mostly found in nucleoli. NPM also involved in the maturation of preribosomes and duplication of centrosomes. Furthermore, it is also active in control and regulation of the ARF-p53 tumor suppressor pathway. A high rate of incidence and prognostic involvement is reported by various authors in AML patients. In AML it behaves as a favorable prognostic marker. NPM mutations are more frequently associated with normal-karyotype AML and are usually absent in patients having abnormal or poor cytogenetic. NPM mutations are not frequent in other hematopoietic tumors. Two main types of mutations have been described to date. Both of these cause abnormal cytoplasmic localization of NPM1. Their high incidence rate in normal karyoptype and their favorable nature m ake those mutations hot spot or front face mutations which should be checked before treatment starts.

• ETRI Journal
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• v.43 no.2
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• pp.209-220
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• 2021

The filtered orthogonal frequency division multiplexing (F-OFDM) system has been recommended as a waveform candidate for fifth-generation (5G) communications. The suppression of out-of-band emission (OOBE) and asynchronous transmission are the distinctive features of the filtering-based waveform frameworks. Meanwhile, the high peak-to-average power ratio (PAPR) is still a challenge for the new waveform candidates. Partial transmit sequence (PTS) is an effective technique for mitigating the trend of high PAPR in multicarrier systems. In this study, the PTS technique is employed to reduce the high PAPR value of an F-OFDM system. Then, this system is compared with the OFDM system. In addition, the other related parameters such as frequency localization, bit error rate (BER), and computational complexity are evaluated and analyzed for both systems with and without PTS. The simulation results indicate that the F-OFDM based on PTS achieves higher levels of PAPR, BER, and OOBE performances compared with OFDM. Moreover, the BER performance of F-OFDM is uninfluenced by the use of the PTS technique.