• Asian-Australasian Journal of Animal Sciences
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• 제33권2호
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• pp.219-229
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• 2020

Objective: Considering the physiological and clinical importance of leptin receptor (LEPR) in regulating obesity and the fact that porcine LEPR expression is not known to be controlled by lncRNAs and miRNAs, we aim to characterize this gene as a potential target of SSC-miR-323 and the lncRNA TCONS_00010987. Methods: Bioinformatics analyses revealed that lncRNA TCONS_00010987 and LEPR have SSC-miR-323-binding sites and that LEPR might be a target of lncRNA TCONS_00010987 based on cis prediction. Wild-type and mutant TCONS_00010987-target sequence fragments and wild-type and mutant LEPR 3'-UTR fragments were generated and cloned into pmiRRB-REPORT^TM-Control vectors to construct respective recombinant plasmids. HEK293T cells were co-transfected with the SSC-miR-323 mimics or a negative control with constructs harboring the corresponding binding sites and relative luciferase activities were determined. Tissue expression patterns of lncRNA TCONS_00010987, SSC-miR-323, and LEPR in Anqing six-end-white (AQ, the obese breed) and Large White (LW, the lean breed) pigs were detected by real-time quantitative polymerase chain reaction; backfat expression of LEPR protein was detected by western blotting. Results: Target gene fragments were successfully cloned, and the four recombinant vectors were constructed. Compared to the negative control, SSC-miR-323 mimics significantly inhibited luciferase activity from the wild-type TCONS_00010987-target sequence and wild-type LEPR-3'-UTR (p<0.01 for both) but not from the mutant TCONS_00010987-target sequence and mutant LEPR-3'-UTR (p>0.05 for both). Backfat expression levels of TCONS_00010987 and LEPR in AQ pigs were significantly higher than those in LW pigs (p<0.01), whereas levels of SSC-miR-323 in AQ pigs were significantly lower than those in LW pigs (p<0.05). LEPR protein levels in the backfat tissues of AQ pigs were markedly higher than those in LW pigs (p<0.01). Conclusion: LEPR is a potential target of SSC-miR-323, and TCONS_00010987 might act as a sponge for SSC-miR-323 to regulate LEPR expression.

• The Korean Journal of Physiology and Pharmacology
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• 제19권5호
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• pp.451-460
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• 2015

Sirtuin 1 (SIRT1) is a mammalian $NAD^+$-dependent protein deacetylase that regulates cellular metabolism and inflammatory response. The organ-specific deletion of SIRT1 induces local inflammation and insulin resistance in dietary and genetic obesity. Macrophage-mediated inflammation contributes to insulin resistance and metabolic syndrome, however, the macrophage-specific SIRT1 function in the context of obesity is largely unknown. C57/BL6 wild type (WT) or myeloid-specific SIRT1 knockout (KO) mice were fed a high-fat diet (HFD) or normal diet (ND) for 12 weeks. Metabolic parameters and markers of hepatic steatosis and inflammation in liver were compared in WT and KO mice. SIRT1 deletion enhanced HFD-induced changes on body and liver weight gain, and increased glucose and insulin resistance. In liver, SIRT1 deletion increased the acetylation, and enhanced HFD-induced nuclear translocation of nuclear factor kappa B (NF-${\kappa}B$), hepatic inflammation and macrophage infiltration. HFD-fed KO mice showed severe hepatic steatosis by activating lipogenic pathway through sterol regulatory element-binding protein 1 (SREBP-1), and hepatic fibrogenesis, as indicated by induction of connective tissue growth factor (CTGF), alpha-smooth muscle actin (${\alpha}$-SMA), and collagen secretion. Myeloid-specific deletion of SIRT1 stimulates obesity-induced inflammation and increases the risk of hepatic fibrosis. Targeted induction of macrophage SIRT1 may be a good therapy for alleviating inflammation-associated metabolic syndrome.

• 보건행정학회지
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• 제28권3호
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• pp.240-250
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• 2018

By the end of 2017, in a world of 7.6 billion people, there were inequalities in healthcare indices both within and between nations, and this gap continues to increase. Therefore, this study aims to understand the current status of regional inequalities in healthcare indices and to find an action plan to tackle regional health inequality through a geo-economic review in Korea. Since 2008, there was great inequality in life expectancy and healthy life expectancy by region in not only metropolitan cities but also districts in Korea. While the community health statistics from 2008-2017 show a continuous increase of inequality during the last 10 years in most healthcare indices related to noncommunicable diseases (except for some, like smoking), the inequality has doubled in 254 districts. Furthermore, health inequality intensified as the gap between urban (metropolitan cities) and rural regions (counties) for rates of obesity (self-reported), sufficient walking practices, and healthy lifestyle practices increased from twofold to fivefold. However, regionalism and uneven development are natural consequences of the spatial perspective caused by state-lead developmentalism as Korea has fixed the accumulation strategy as its model for growth with the background of export-led industrialization in the 1960s and heavy and chemical industrialization in the 1970s, although the Constitution of the Republic of Korea recognizes the legal value of balanced development within the regions by specifying "the balanced development of the state" or "ensuring the balanced development of all regions." In addition, the danger of a 30% decline or extinction of local government nationwide is expected by 2040 as we face not only a decline in general and ageing populations but also the era of the demographic cliff. Thus, the government should continuously operate the "Special Committee on Regional Balanced Development" with a government-wide effort until 2030 to prevent disparities in the health conditions of local residents, which is the responsibility of the nation in terms of strengthening governance. To address the regional inequalities of rural and urban regions, it is necessary to re-adjust the basic subsidy and cost-sharing rates with local governments of current national subsidies based mainly on population scale, financial independence of local government, or distribution of healthcare resources and healthcare indices (showing high inequalities) overall.

• International Journal of Human Ecology
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• 제13권2호
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• pp.53-67
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• 2012

This study uses the 1999-2009 Korean Time Use Survey to explore how mothers' employment affects parental time spent in activities that may relate to their children's weight. Specifically, it estimates two-part regression models to assess the effects of maternal employment upon the amount of time mothers spend in activities potentially related to their preschool children's eating and physical activity. The findings suggest that working mothers and those mothers who work longer hours allocate significantly less time in food preparation, eating with the child, and supervising the child's physical activity than mothers who are not employed and those who work fewer hours. The paper also finds that spouses of working mothers do not allocate more time to these activities to offset the reduction in mothers' time. Also, using local unemployment rates as instrumental variables, maternal employment is found to allow mothers to spend significantly more time on food preparation and family meals, although its effect on mothers' total childcare time is negative. Several aspects of Korean society may have made the relationship between maternal employment and childhood weight issues different from what was found in other countries.

• 보건교육건강증진학회지
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• 제24권4호
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• pp.219-230
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• 2007

The purpose of this study was to identify the current problems of school health education policies and practices in Korea, and to establish the strategies to improve the effectiveness and efficiency of school health education program. The severity of adolescents's health problems including obesity, smoking, drug abuse, teen pregnancy, etc has been increased recently and coping strategies to deal with these problems became urgent. The role of school as a key setting for health education should be empathized. However, there were limitations for the effectiveness of school health education in Korea because of the lack of recognition about the importance, guiding principles of the school health education by the school health related law, life skill-focused curriculum, capacity of teachers for health education, and linkage between school and community. In order to improve the effectiveness of school health education, establishment of infrastructure, national and local health education standard, and operating principles for the school health education program should be provided. Life skill-focused health education curriculum should be developed for the effective health education. Teacher training and education also should be the essential component of school health education program. For the improvement of efficiency in school health education practices, cooperation with family and community support system would be necessary.

• 한국패션뷰티학회지
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• 제4권1호
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• pp.35-41
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• 2006

From 1995 to 2005 the information related to clothing and textiles publicized by 6 journals regarding Elder Women's body type was collected and analyzed. The results are as follows. 1. It has been investigated into the elder woman's body type since 1995, and this subject needs a detailed study. in audition, investigation into woman's body type has been studied parts of the body for clothing construction. 2. Analysis result for measure area preponderance(Seoul area and local metropolitan city) of area, measure subject number mainly to large scale group more than 300 people that measure is consisting most know can. 3. Where the way of measurement is concerned, there are direct measurements more than indirect measurements. In case of using just direct measurement, the study often mentions necessity of indirect measurements. Therefore it is thought simultaneous measurement is the most desirable. 4. A Body type characteristic of elder woman shows a size of every part diminishes as growing older. Owing mainly to obesity of waist and abdomen, they have a changed body a measure of around the abdomen is bigger than around the hips. hips drop and the upper half of the body down, and besides, feet are changed into the flat.

• Journal of Information Processing Systems
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• 제10권3호
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• pp.395-411
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• 2014

Temporal medical data is often collected during patient treatments that require personal analysis. Each observation recorded in the temporal medical data is associated with measurements and time treatments. A major problem in the analysis of temporal medical data are the missing values that are caused, for example, by patients dropping out of a study before completion. Therefore, the imputation of missing data is an important step during pre-processing and can provide useful information before the data is mined. For each patient and each variable, this imputation replaces the missing data with a value drawn from an estimated distribution of that variable. In this paper, we propose a new method, called Newton's finite divided difference polynomial interpolation with condition order degree, for dealing with missing values in temporal medical data related to obesity. We compared the new imputation method with three existing subspace estimation techniques, including the k-nearest neighbor, local least squares, and natural cubic spline approaches. The performance of each approach was then evaluated by using the normalized root mean square error and the statistically significant test results. The experimental results have demonstrated that the proposed method provides the best fit with the smallest error and is more accurate than the other methods.

• 대한수의학회지
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• 제56권4호
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• pp.229-232
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• 2016

Mammary gland tumors are very rare in male dogs. In this study, four mammary gland tumors from 3 male dogs (2 intact, 1 neutered) were collected from local animal hospitals. The dogs included two purebred Shih Tzu (1 intact, 1 neutered) and one intact purebred Cocker Spaniel. The mean age of dogs with mammary gland tumors was 9 years (5-12 years). Two dogs had a solitary mass, whereas one dog had two mammary masses. Of the four tumor masses, three were observed in the fourth or fifth mammary glands, and one was observed in the third mammary gland. According to histopathologic examinations, all four mammary masses from three dogs were benign tumors including two benign mixed tumors in one case and two complex adenomas. There were no history of obesity, testicular tumors, diabetes, and sex hormonal therapy in any male dogs with mammary tumors. Surgical excision was the only reported treatment for these tumors. No recurrence or metastasis was recorded up to 25 months after surgery.

• Food Quality and Culture
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• 제3권2호
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• pp.59-63
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• 2009

As one of study efforts to develop a food product that can satisfy the taste of modern people and increase the usage of the popular local product of walnut in the region of Chunan, the current study developed a no sugar added functional walnut-sulgi. The walnut-sulgi was manufactured by adding walnut powder into typical sulgi rice cake. While manufacturing the walnut-sulgi rice cake, the health beneficial sugar alcohol ingredient of sorbitol was added instead of commonly used sugar to specialize the walnut-sulgi as a functional rice cake. As the result of such effort, a soft and moist walnut-sulgi was produced. The color of the newly developed walnut-sulgi is white and has a pleasing taste, and its consumer acceptability was higher than the sugar added walnut-sulgi by showing much soft sweetness and textural properties. Considering the facts that sorbitol has a lower glycemic index (GI) than sugar and the content of unsaturated fatty acids that are insufficient in rice cake could be increased, the newly developed sorbitol added walnut-sulgi is thought to be an appropriate functional rice cake that can attractively appeal to obesity and diabetes concerning modern people.

• 융합정보논문지
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• 제8권3호
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• pp.17-24
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• 2018

본 연구는 도시지역 근로자 5,638명을 대상으로 건강행태와 만성질환 유병률과의 관련성을 파악하고자 하였다. 자료분석은 빈도, 백분율, 교차분석을 실시하고 만성질환에 미치는 영향을 분석하기 위하여 다중로지스틱 회귀분석을 사용하였다. 연구 결과 대상자의 건강행태는 남자가 흡연과 음주가 높게 나타났고, 운동은 남녀 모두 추천건강운동량 기준으로 낮게 나타났으며 만성질환 유병률은 비만과 고지혈증이 높게 나타났다. 만성질환에 영향을 미치는 중요 인자는 연령, 운동, 음주로 확인되었다. 본 연구의 결과는 국가, 지역사회 및 산업현장에서 바람직한 건강행태 형성을 위한 체계적인 건강관리프로그램을 운영할 필요가 있음을 시사한다.