• International Journal of Oral Biology
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• v.30 no.1
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• pp.17-22
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• 2005

In the previous studies of Saccharomyces cerevisiae, Abp140p (actin binding protein 140) fused to GFP has been only a protein that can label actin cables of yeast cells so far. However, the role of Abp140p in actin dynamics was remained elusive. In this study, the function of Abp140p was investigated with a deletion mutant and overexpression of GFP fused Abp140p. The deletion mutant was slightly more susceptible to Latrunculin-A (Lat-A), an actin-monomer sequestering agent, than wild type, although no significant deformation of actin structures was caused by ABP 140 deletion. Overexpression of Abp140p-GFP retarded cell growth, and produced thick and robust actin cables. Lat-A was not able to destabilize the thick actin cables, which suggests that actin dynamics was compromised in the cells with surplus of Abp140p. Therefore, Abp140p seems to stabilize actin cables together with other bundling proteins. Recently, actin cable dynamics of budding yeast was found to have a resemblance to that of filopodial tip of cultured mammalian cells. Retrograde movement of actin cables from buds to mother cells indicated local generation of the cable at bud sites. By using Abp140p-GFP, we traced the steps in the generation of a new actin cable after elimination of old cables by sodium azide. Before the appearance of a new actin cable, Abp140p-GFP concentrated in buds and disappeared, as mother cells became abundant in actin cables. Our observations provide a direct evidence of actin cable formation at buds of budding cells.

• Geomechanics and Engineering
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• v.23 no.5
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• pp.441-454
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• 2020

The tunnel collapse, large deformation of surrounding rock, water and mud inrush are the major geological disasters in soft rock tunnel construction. Among them, tunnel collapse has the most serious impact on tunnel construction. Current research backed theories have certain limitations in identifying the collapse risk of soft rock tunnels. Examining the Zhengwan high-speed railway tunnel, eight soft rock tunnel collapse influencing factors were selected, and the combination of indicator weights based on the analytic hierarchy process and entropy weighting methods was obtained. The results show that the groundwater condition and the integrity of the rock mass are the main influencing factors leading to a soft rock tunnel collapse. A comprehensive fuzzy evaluation model for the collapse risk of soft rock tunnels is being proposed, and the real-time collapse risk assessment of the Zhengwan tunnel is being carried out. The results obtained via the fuzzy evaluation model agree well with the actual situation. A tunnel section evaluated to have an extremely high collapse risk and experienced a local collapse during excavation, verifying the feasibility of the collapse risk evaluation model. The collapse risk evaluation model proposed in this paper has been demonstrated to be a promising and innovative method for the evaluation of the collapse risk of soft rock tunnels, leading to safer construction.

• Journal of the Korean Chemical Society
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• v.38 no.1
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• pp.1-7
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• 1994

Semiempirical MNDO calculations are employed to study ionicity of OH groups and stability in $T_1-OH-T_2bridges(T_1$ = Al, B and $T_2$ = P, Si) such as found in aluminophosphate family($AlPO_4-5$, BAPO-5, and SAPO-5) molecular sieves. Dimeric model clusters of Al-OH-P, B-OH-P and Al-OH-Si bridges were considered. It is shown that the elongation of the T-O bond, upon replacement of Al by B, occurs preferentially by a local deformation of the Al-O-P bridge. But the elongation of the T-O bond occurs preferentially by a rotation of Al-O-Si bridge upon substitution P for Si. Also, the ionicity of OH groups and stability increase in order to B-OH-P < Al-OH-P < Al-OH-Si bridge.

• Journal of the Society of Naval Architects of Korea
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• v.32 no.3
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• pp.98-106
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• 1995

In ship and offshore structures, there exist many local structural systems which may be regarded as a combined structural systems composed of thick plates or double wall panels and attachments reducible to damped spring-mass systems. For vibration analysis of such a combined system an analytical method based on the receptance method is presented in this paper. The free vibrational characteristics and forced vibration responses of the combined system can be calculated by synthesis of receptances of the panel and attachments. To calculate receptances of the panel, it may be regarded as a Mindlin plate for consideration of effects of shear deformation and rotary inertia and the assumed mode-Lagrange's equation method is applied using Timoshenko beam function or polynomials having properties of Timoshenko beam function as trial functions. Through some numerical calculations, accuracy and efficiency of the presented method are shown.

• Journal of the Earthquake Engineering Society of Korea
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• v.6 no.3
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• pp.31-40
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• 2002

The purpose of this study is to investigate the seismic behavior of RC bridge piers and to provide the data for developing improved seismic design criteria. The accuracy and objectivity of the assessment process may be enhanced by the use of sophisticated nonlinear finite element analysis program. A computer program, named RCAHEST(reinforced concrete analysis in higher evaluation system technology), for the analysis of reinforced concrete structures was used. Material nonlinearity is taken into account by comprising tensile, compressive and shear models of cracked concrete and a model of reinforcing steel. The smeared crack approach is incorporated. n boundary plane at which each member with different thickness is connected, local discontinuity in deformation due to the abrupt change in their stiffness can be taken into account by introducing interface element. The effect of number of load reversals with the same displacement amplitude has been also taken into account to model the reinforcing steel and concrete. In the companion paper, the proposed numerical method for seismic damage evaluation of RC bridge piers is verified by comparison with the reliable experimental results.

• 한국지구물리탐사학회:학술대회논문집
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• 2003.11a
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• pp.255-261
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• 2003

Tunnelling in poor and heterogeneous ground is a difficult task. Even with a good geological investigation, uncertainties with respect to the local rock mass structure will remain. Especially for such conditions, a reliable short-term prediction of the conditions ahead and outside the tunnel profile are of paramount importance for the choice of appropriate excavation and support methods. The information contained in the absolute displacement monitoring data allows a comprehensive evaluation of the displacements and the determination of the behaviour and influence of an anisotropic rock mass. Case histories and with numerical simulations show, that changes in the displacement vector orientation can indicate changing rock mass conditions ahead of the tunnel face (Schubert & Budil 1995, Steindorfer & Schubert 1997). Further research has been conducted to quantify the influence of weak zones on stresses and displacements (Grossauer 2001). Sellner (2000) developed software, which allows predicting displacements (GeoFit$\circledR$). The function parameters describe the time and advance dependent deformation of a tunnel. Routinely applying this method at each measuring section allows determining trends of those parameters. It shows, that the trends of parameter sets indicate changes in the stiffness of the rock mass outside the tunnel in a similar way, as the displacement vector orientation does. Three-dimensional Finite Element simulations of different weakness zone properties, thicknesses, and orientations relative to the tunnel axis were carried out and the function parameters evaluated from the results. The results are compared to monitoring results from alpine tunnels in heterogeneous rock. The good qualitative correlation between trends observed on site and numerical results gives hope that by a routine determination of the function parameters during excavation the prediction of rock mass conditions ahead of the tunnel face can be improved. Implementing the rules developed from experience and simulations into the monitoring data evaluation program allows to automatically issuing information on the expected rock mass quality ahead of the tunnel.

• Nuclear Engineering and Technology
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• v.44 no.7
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• pp.791-798
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• 2012

Since standardized fracture test specimens cannot be easily extracted from in-service components, several alternative fracture toughness test methods have been proposed to characterize the deformation and fracture resistance of materials. One of the more promising alternatives is the local approach employing the SP(Small Punch) testing technique. However, this process has several limitations such as a lack of anisotropic yield potential and tediousness in the damage parameter calibration process. The present paper investigates estimation of ductile fracture resistance(J-R) curve by FE(Finite Element) analyses using an anisotropic damage model and enhanced calibration procedure. In this context, specific tensile tests to quantify plastic strain ratios were carried out and SP test data were obtained from the previous research. Also, damage parameters constituting the Gurson-Tvergaard-Needleman model in conjunction with Hill's 48 yield criterion were calibrated for a typical nuclear reactor material through a genetic algorithm. Finally, the J-R curve of a standard compact tension specimen was predicted by further detailed FE analyses employing the calibrated damage parameters. It showed a lower fracture resistance of the specimen material than that based on the isotropic yield criterion. Therefore, a more realistic J-R curve of a reactor material can be obtained effectively from the proposed methodology by taking into account a reduced load-carrying capacity due to anisotropy.

• Journal of Korean Society of Steel Construction
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• v.9 no.3 s.32
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• pp.431-440
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• 1997

The paper describes a theoretical model for the prediction of bridge temperatures from meteorological data measured at bridge site and local meteorological center together with existing finite element heat transfer theory and solar radiation transfer theory to determine the time dependent temperature distribution of bridge. In this analytical model, the most adequate equation for the calculation of solar radiation on the bridge surface, which is dominant in day time is described based on the results of several experimental studies for the solar energy. The validity of this model is tested against field data obtained from long term experimental program on Sadang Viaduct in Seoul. Also, this paper describes the linear correlation between design variables and meteorological data to establish analytical criteria for the prediction of the average temperature, which are responsible for the longitudinal deformation of the bridges and of the vertical differential temperature profiles. which are responsible for the bending deformations from the long term experimental results.

• Journal of Korean Society of Steel Construction
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• v.13 no.1
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• pp.9-18
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• 2001

many steel tubular truss as roof structures are used of the large span structures Steel tubular sectioned truss has the structural merits in compared with other sections such as H, L-shape sections However it occurs local buckling at the joint of branch in truss and it makes the deterioration of loading capacity Loading capacity and deformation characteristics of truss joints are very complicate so it is very hard to predict exact solution of them Therefore this thesis dealt with T-type joints of steel circular hollow sectioned truss. A series of experimental scheme were planned and mainly experimental parameters were : ratio of diameter of branch-diameter of main chord(d/D). diameter-thickness(T/D) of main chord. In this paper predicted yield load capacity using by closed ring analysis method additionally compared with that of suggested by closed ring analysis method additionally compared with that of suggested by other countries.

• Journal of Mechanical Science and Technology
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• v.19 no.10
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• pp.1919-1931
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• 2005

Human joint motion can be kinematically described in three planes, typically the frontal, sagittal, and transverse, and related to experimentally measured data. The selection of reference systems is a prerequisite for accurate kinematic analysis and resulting development of the equations of motion. Moreover, the development of analysis techniques for the minimization of errors, due to skin movement or body deformation, during experiments involving human locomotion is a critically important step, without which accurate results in this type of experiment are an impossibility. The traditional kinematic analysis method is the Angular-based method (ABM), which utilizes the Euler angle or the Bryant angle. However, this analysis method tends to increase cumulative errors due to skin movement. Therefore, the objective of this study was to propose a new kinematic analysis method, Position-based method (PBM), which directly applies position displacement data to represent locomotion. The PBM presented here was designed to minimize cumulative errors via considerations of angle changes and translational motion between markers occurring due to skin movements. In order to verify the efficacy and accuracy of the developed PBM, the mean value of joint dislocation at the knee during one gait cycle and the pattern of three dimensional translation motion of the tibiofemoral joint at the knee, in both flexion and extension, were accessed via ABM and via new method, PBM, with a Local Reference system (LRS) and Segmental Reference system (SRS), and then the data were compared between the two techniques. Our results indicate that the proposed PBM resulted in improved accuracy in terms of motion analysis, as compared to ABM, with the LRS and SRS.