• 제목/요약/키워드: loach eggs

검색결과 39건 처리시간 0.022초

유전자 변환 동물 생산을 위한 Plasmid Vector(pSS4)의 개발 (Development of a Plasmid Vector(pSS4) for the Use in Animal Transgenesis)

  • 전진태;이상호;박성수
    • 한국가축번식학회지
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    • 제17권3호
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    • pp.263-267
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    • 1993
  • Transgenic animals have become an important tool in the basic and applied sectors of genetic and biomedical sciences. In particular transgenes provide clear-cut markers in the spatial and temporal analysis of developing embryos for the understanding of developmental mechanisms. For the long-term use of plasmid vector in a particular purpose it would be necessary to develop one's own vector system which can be properly expressed in eukaryotic system. Plasmids were constructed from ori region of pUC19 and early region of SV40 through various steps. LacZ gene coding for $\beta$-galactosides was fused to early gene of SV40 in translational in-frame. Poly(A) tailing site of SV40 was inserted at the 3' lacZ so that initiation, elongation and terminatin be controlled by SV40 transcription (pSS4). Biological function of the constructed pSS4 was demonstrated via microinjection of the plasmid into fertilized loach eggs and subsequent detection of $\beta$-galactosidase in developing embryos. The result indicate that the newly constructed pSS4 is functional in a eukaryotic system in vivo. Thus pSS4 may be used as an efficient tool for the study of embryogenesis and a basic carrier for various genes for animal transgenesis.

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치리(Cultriculus eigenmanni)의 형태 및 생활사에 관한 연구 I. 난발생과 부화자어에 대하여 (Studies on the Morphological Characteristics and Life History of the Chili Fish, Cultriculus eigenmanni (Jordan et Metz) (1) On the Egg Development and the Fry in the Larval Stage)

  • 김을배;김영길
    • 한국수산과학회지
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    • 제17권2호
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    • pp.151-156
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    • 1984
  • 1983년 6월 25일부터 7월 19일 사이에 전북익산군 오산면 남전리와 옥구군미성읍 군산외항 부근수로에서 채집한 치리의 친어에 태반성 성선자극 hormone "Puberogen" 1,000 unit 용액을 자웅 다 같이 미당 0.1cc씩을 강복에 주사한 후 사육수조($30{\times}50{\times}70cm$)내에 수용하여 자연산란 또는 인공수정시킨 수정란을 수온 $23.5{\sim}25.0^{\circ}C$에서 부화관리하면서 난발생과 부화자어에 대한 관찰을 하고 다음과 같은 결과를 얻었다. 1. 수정란은 분리침성부착난으로서 난경 $1.38mm{\sim}l.59mm$의 구형이며, 난황은 담황색으로 불투명하였으며 난황의 직경은 $1.17{\sim}l.27mm$이었다. 2. 수온 $23.5{\sim}25.0^{\circ}C$ 범위에서 수정후 29시간 50 분만에 부화되기 시작하였는데 일반적으로 발생 속도가 빠른 편이며, 부화전에는 체색소포 및 안구가 형성되지 않고 있으며, 혈액순환도 일어나지 않았다. 3. 부화직후의 자어는 전장이 $3.87{\sim}3.99mm$였으며, 난황주머니의 길이는 2.39mm로 근절수는 28+15=43 이었다. 4. 부화후 1일(20시간)에 전장 4.73mm로 성장하고, 꼬리지느러미와 가슴지느러미, 항문이 형성되기 시작했으며, 난황주머니 상변에 체색소포가 출현하기 시작하였다. 5. 부화후 2 일 (50시간)에는 전장 5.02 mm로 되었으며, 북부막 지느러미는 난황주머니의 3분의 2 앞부위까지 신장되며, 안구나 입이 형성되기 시작하였다. 6. 부화후 3일(77시간)이 되면 전장 5.42mm까지 성장하고, 난황은 거의 흡수하여 자어는 완전히 부상유영하였으며, 혈액순환도 일어나게 되었다. 또한 아가미와 소화관이 분화되며, 등지느러미를 제외한 각 지느러미의 원기가 발달되고 안구도 완성되었다. 7. 부화후 5일(137시간)에 전장은 5.68mm로 등지느러미 원기가 생기며, 각 기관이 거의 형성되고 입의 상,하악이 움직이면서 유영운동이 활발하였다.

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멸종위기어류 얼룩새코미꾸리 Koreocobitis naktongensis (Cobitidae)의 수조 내 산란행동 (The Spawning Behaviour of the Endangered Freshwater Fish Koreocobitis naktongensis (Cypriniformes: Cobitidae) under Artificial Conditions)

  • 홍양기;양현;방인철
    • 한국어류학회지
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    • 제33권1호
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    • pp.15-21
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    • 2021
  • 멸종위기에 처한 우리나라 고유 담수어류인 얼룩새코미꾸리 Koreocobitis naktongensis의 호르몬 처리에 의한 산란행동을 관찰하기 위하여 2010년 4월 20~27일까지 산란시기에 성 성숙 호르몬(Ovaprim)을 주사하여 산란행동을 관찰하였다. 산란행동을 분석한 결과 산란 전 행동, 산란행동, 산란 후 행동으로 구분되었다. 산란 전에는 주로 휴식과 유영을 반복하였다. 호르몬 주사 후 평균적으로 11~12시간 경과하면 산란행동을 시작하여 암·수 1 : 1로 1~18회 산란하였고, 수중에서 수컷이 골질반을 이용하여 암컷의 등지느러미 뒷부분의 몸을 원의 형태로 감싸안고 산란이 이루어졌다. 산란 후 행동에서는 특별한 난 보호행동은 보이지 않았으며 산란에 참여하지 않은 개체들이 수정란 일부를 섭식하는 것이 관찰되었다.

북한강 지류 상천천에 서식하는 대륙종개 Barbatula nuda (Nemacheilidae)의 산란특성과 수조 내 산란행동 (Spawning Behavior and Characteristics of Siberian Stone Loach, Barbatula nuda (Nemacheilidae) in Sangcheoncheon Stream, a Tributary of Bukhangang River, Korea)

  • 곽영호;김정배;송하윤
    • 한국어류학회지
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    • 제35권1호
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    • pp.20-29
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    • 2023
  • 대륙종개의 산란특성과 산란행동을 규명하기 위해 경기도 가평군 청평면에 위치한 북한강의 지류 상천천에서 2021년 3월부터 5월까지 7회 조사하였다. 전체 조사기간 동안 대륙종개 890개체가 채집되었으며 성비(♂:♀)는 1 : 1.34로 수컷이 암컷보다 많았다. 상천천의 대륙종개는 수온 12~15.7℃인 3월 15일~4월 24일에 산란하였다. 최소 성적성숙개체는 전장 56.7 mm였고 만 1년생 개체였으며, 전장 80.5 mm 이상 개체부터 산란이 관찰되었고 만 2년생 개체였다. 50% 군성숙전장은 67.2 mm로 나타났다. 대륙종개의 복강에 HCG호르몬 10 IU/g으로 주사한 결과, 약 15시간 후에 산란이 유도되었으며 수컷이 암컷의 몸을 원형으로 감는 산란행동이 관찰되었다. 산란 후 암컷과 수컷은 바닥에서 휴식을 취하였고 바닥에 떨어진 수정란은 산란에 참여하지 않은 개체들에게 일부 섭식되었다.

제주지역(濟州地域)의 식품금기(食品禁忌)에 관(關)한 연구(硏究) (I)-임신기(妊娠期)를 중심(中心)으로- (A Study of Food Taboos on Jeju Island (I)-Focused on Pregnancy-)

  • 김기남;모수미
    • Journal of Nutrition and Health
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    • 제10권1호
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    • pp.49-58
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    • 1977
  • Nutrition counselors in Korea often encounter difficulty in their attempt to change village women's attitudes regarding food taboos which are counter to good eating habits. There are a great many food superstitions which are not due to religious influence, but seem to be related to shape and composition of food. Many expectant mothers superstitiously avoid eating certain foods for fear that they may cause mental or physical abnormality in their babies. As was shown in a previous survey (Mo, 1966)of villages in all provinces except Jeju Island, such superstitions were common among pregnant and lactating mothers. Many food taboos and superstitions based on non-scientific and irrational ideas do exist even in modern society, and are a major obstacle to nutritionally adequate food consumption. A study of food taboos among women of Jeju Island was undertaken from November to December of 1976, these results to be compared as well with those of the previous study. There were 73 items found to be prohibited during pregnancy. Of these, 48.7% were of the deaf group, 17.4% fish, 5.5% eggs, 4.7% cereal, and only 2.2% fruit. Of 252% women respondents, 111 (45% ) abstained from eating chicken, duck, and shark because of the belief that they would cause their babies to be born with gooseflesh or shark skin. Many of them avoided rabbit meat for fear that their babies might be born with harelip. It was also feared that a baby would become disfigured if his mother ate duck, goat, dog meat, chicken or duck eggs, or soup made of bones. A common superstition was that highly spiced or salty foods would cause the fetus to be hairless. Squid and octopus were believed to cause babies to have weak bones, or none at all. Most of these food taboos were associated with fears concerning Physical structure and appearance of unborn babies. Other taboos were associated with fear of undesirable behavioral characteristics. For example, some mothers thought that a baby would pinch or bite the mother's breast during the weaning period, if crab meat were eaten during pregnancy. Unevenly sliced rice cake, loach, snake meat and eel were also believed to cause a baby to be ill-tempered. The findings of this study are remarkably similar to those of the previous study conducted by the authour in 1966. Most of the same food taboos, based on non-scientific and irrational reasons, were found on Jeju Island as on the peninsula, and thor were similarly wide-spread. The results of correlational analysis show that the most significant factors related to prevalence of food taboos, are level of education and religious background. Number of food taboos is correlated with level of education. Also, food taboos are least freqent among the Christian woman. Proper nutrition education should he undertaken in order to encourage intake of protein-rich food, particularly during pregnancy when nutritional needs of mother and fetus are great.

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북방종개 Cobitis pacifica(Pisces: Cobitidae)의 배 발생과 초기생활사 (Embryonic Development and Early Life History of the Northern Loach, Cobitis pacifica (Pisces: Cobitidae))

  • 이완옥;김경환;백재민;강용진;전형주;김치홍
    • 생태와환경
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    • 제44권1호
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    • pp.1-8
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    • 2011
  • 미꾸리과 어류에 속하는 우리나라 고유종인 북방종개를 강원도 고성군 북천에서 채집하여 난발생과정과 초기 생활사를 연구하였다. 채집된 성숙한 북방종개를 호르몬(LHRH-a, HCG) 주사하여 채란하고 건식법으로 수정 시켰다. 수정란은 원형이었고 엷은 흰색을 띠는 분리침성란이며, 난경은 $1.09{\pm}0.04\;mm$ (n=20) 이었다. 수온 $21.0{\sim}24.0^{\circ}C$에서 수정 후 48시간 전후하여 부화하였으며, 부화자어의 크기는 전장 $2.87{\pm}0.05\;mm$ (n=20) 이었다. 부화 4 일 후에는 전장 $6.86{\pm}0.10\;mm$ (n=10)로 성장하였고, 난황이 거의 흡수되고 입과 항문이 열렸다. 부화 14 일 후에는 전장 $10.71{\pm}0.34\;mm$ (n=10)로 자라고 대부분의 지느러미 기조가 출현하였으며 반문이 나타났다. 부화 26일 후에는 전장 $14.88{\pm}0.45\;mm$ (n=10)로 자라고 모든 지느러미의 기조수가 정수에 도달하여 치어기로 이행되었다. 부화 80일 후에는 전장 $33.3{\pm}1.25\;mm$ (n=10)로 성장하였으며, 반문의 모양과 외부형태가 성어와 유사하였다. 북방종개의 배 발생 및 초기생활사 특정은 다른 미꾸리과 어류들과 큰 차이를 보이지 않았으며, 추후 고유종의 복원연구에 기초자료를 제공하였다.

줄종개 Cobitis tetralineata (Pisces; Cobitidae)의 개체군 생태 (Population Ecology of Korean Sand Loach Cobitis tetralineata (Pisces; Cobitidae) in the Seomjin River, Korea)

  • 김익수;고명훈;박종영
    • Journal of Ecology and Environment
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    • 제29권3호
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    • pp.277-286
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    • 2006
  • 줄종개, Cobitis tetralineata의 생태를 섬진강 지류인 추령천에서 조사하였다. C. tetralineata는 하상이 모래이고, 유속이 $10{\sim}20cm/see$, 수심은 $30{\sim}150cm$인 곳에 대부분 서식하였다. 활동은 낮에만 활동하는 주행성 어류로 3월부터 10월까지 활동하였고, 이후 11월부터 이듬해 2월까지는 바닥 속으로 파고들어가 월동하였다. 성비는 암컷과 수컷이 1 : 0.57로 나타났고, 암컷이 수컷보다 $20{\sim}30mm$(TL)이 더 컸다. 줄종개(암컷)의 연령은 $20{\sim}40mm$가 당년생, $45{\sim}65mm$가 1년생, $65{\sim}90mm$가 2년생, 90 mm 이상이 3년생 이상으로 추정되었다. 부화 후 $13{\sim}14$개월 이후에 수컷 가슴지느러미 제2기조에 골질반이 형성되어 암수가 구별되었으며, 또한 수컷은 산란시기에 반문변 이가 일어났다. 산란시기는 수온이 $22{\sim}26^{\circ}C$인 6월 말부터 7월 중순으로 추정되었고, 포란수는 $1,288{\pm}583(474{\sim}2,976)$개, 난경은 $0.98{\pm}0.1mm$이었다. 섭식은 주로 깔다구류, 꽃병벌레류, 물벼룩류, 조류 등을 섭식하였으며, 섭식률은 4월과 9월에 높게 나타났고 월동기에는 섭식하지 않았다.

이비인후, 안, 치아 및 피부증상의 민간요법에 관한 고찰 (Study on the Herb Remedies of ENT, Eyes, Teeth and Skin Problems)

  • 조결자;강현숙
    • 동서간호학연구지
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    • 제1권1호
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    • pp.50-71
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    • 1997
  • The intention of this study is to apprehend the con. tents and methods of herb remedies that are commonly conducted when there are health-problem cases of ENT, eyes, teeth and skin. Methods of this study are divided into two stages : 1) For a period of six months from December 1994 to June 1995, some 40 persons who are believed to be well versed with herb remedies have been randomly chosen, and we made a survey on herb remedies by symptoms: and 2) we have endeavored to make their grounds evident through the studies on literatures with the focus on the basic data collected. Their results are as follows: 1) When one feels a pain in ears, such herb remedies are employed as pouring the vapor into ears, which is made by steaming Alaska pollack, or as applying or wiping with the juices of radish or the ginkgo, or' alum. Applying the radish juice is effective for sterilization and fever removal: and applying the ginkgo juice is effective for cleanliness. But, plastering alum, sesame oil or castor oil, or pouring the vapor of Alaska pollack into ears are perhaps effective but do not have any pharmacologic grounds. 2) When one bleeds at the nose, such kinds of herb remedies are applied as stimulating nose or head with cold water, pressing nose or ridge of nose, or filling up nares with mashed mugworts. In addition, they have utilized garlic or leeks. Such methods as stimulating with cold water or just pressing nose and ridge of nose is based on reasonable grounds, i.e. vasoconstriction and vascular compression ; and applying mashed garlic on the sole of foot is good for the circulation of Qui ; and the use of mugworts and leeks is based upon the pharmacological function of hemostasis. 3) When one feels a sore throat such kinds of herb remedies are employed as gargling or rinsing throat with brine, drinking hot gruel or water, or drinking the juice of mugwort, radish, ginger or Chinese quince. Gargling with brine or drinking the juice of mugwort, radish or ginger is based upon the pharmacological function of pain alleviation, fever removal, and detoxication. 4) When a boil is formed in mouth, such herb remedies are applied as spreading honey, brine or alum water, and taking gall nut, Chinese matrimony vine, lotus root, etc, for drugs. Spreading honey, brine or water that is made by infusing gallnut, Chinese matrimony vine, lotus root is based upon such functions as hematosis, astriction, antibacterial, and antiphlogistic, Alum, eggplant and licorice are said to be effective, but their pharmacological effects have no grounds. 5) When one has conjunctivitis such herb remedies are commonly applied as irrigation with brine and dropping breast milk in eyes. Moreover, such other drugs are used as plantain. shepherd's purse, and purslane, etc. The use of brine, breast milk, plantain, shepherd's purse and purslane is based upon such functions as sterilization, antiphlogistic, disinfection and pain relieving. Eriocaulon sieboldianum, bean stem, bean pod and narcissus leaves are said to be effective, but their pharmacological action have no basis. When one has a stye, such herb remedies are applied as extracting eyelashes, stimulating by a massage of middle finger, third finger or big toe, as well as sear ing with a heated bamboo comb that is fine-toothed. Other than these, plantain and nightshade's nuts are used as drugs for it. Extracting eyelashes corresponds with exclusing suppurative node and draining the stye of pus ; and the use of plantain is based upon disinfection: and nightshade's nuts are said to be effective, however, their pharmacological action has no grounds. 6) For a treatment of toothache, such herb remedies are commonly employed as rinsing mouth with brine and holding cold water or gasoline in the mouth ; and as the drugs that are believed to be effective have been Welsh onion, ginger and castor-oil, plant, etc. The use of Welsh onion is based upon pain killing, antiinflammatory actions, and the use of ginger is based upon detoxication and disinfection ; and seeds of castor-oil plants are said to be effective, but they have no pharmacological basis. 7) When one has hives, such herb remedies are commonly applied as rubbing burned straw in affected parts, exposing to its smoke, rubbing with salt, sweeping down with a broom, and spreading and drinking boiled water of trifoliate orange. The use of cassia tora seeds, walnut, aloe and radish is said to be effective. The use of cassia tor a seeds has the functions of intestinal order, anti-paralysis, etc. The use of walnut has resulted in an increase of blood by invigorating spirits ; and the use of aloe is based upon disinfection, antibiotic, anti-salt, antihistamine and detoxication action. But, the effects of radish juice and straw's smoke have no pharmacological grounds. 8) When one gets a boil, such herb remedies are commonly used as applying a plaster, paste of flour mixed with yolk, soy sauce or honey, as well as spreading pounded elm tree. Other remedies that have been said to be effective are ; heating with mugwort, brine, wild rocambole, aloe, onion, squid's bone, etc. The use of mugwort is based upon pain killing, astringent antiinflammatory and tranquility. Wild rocambole is based upon the generation and maintenance functions of cell-joining textures ; elm tree upon antiphlogistic ; aloe upon fever removal and antiphlogistic ; onion on pain killing, fever removal, antiphlogistic and tranquility ; squid's bone on astriction: and brine or vinegar on sterilization. Pine resin and gardenia seed are said to be effective, but they have no pharmacological basis. 9) When one cuts his skin, such herb remedies are commonly employed as spreading mugwort's juice or squid's bone powder, or pressing the wounds. In addition, kalopanax, onion and fine soil are employed. The use of mugwort, kalopanax and squid's bone is based upon such functions as hemostasis, sedation, pain killing, antibacterial ; and fine soil is said to be effective, but it has no pharmacological basis. 10) When one suffers from whitlow, such herb remedies are commonly utilized as heating with boiled soy sauce, spreading soybean paste, or dipping into eggs, etc. Other drugs that have been employed are onion root, brine, eggplant, potato, loach, etc. The use of onion is based upon pain killing and antiphlogistic functions ; and that of brine upon antiphlogistic function. The use of soy sauce or soybean paste, fomentation, eggplant, potato and loach is said to be effective, but it has no pharmacblogic ground. 11) For the treatment of frostbite, such herb remedies are commonly used as dipping the affected part into frozen soybean sack, using boiled water of eggplant stem, garlic caulis, onion, hot pepper, caulis. Onion is based upon antiphlogistic and tranquility actions garlic upon disintection, metabolic exacerbation, tonic and aphrodisiac actions and the use of eggplant and hot pepper is based upon help blood circulation, dissolution and excretion of waste matters in vein. 12) For the treatment of burn, such herb remedies or drugs are commonly used as cleansing with Korean gin, spreading eggs, cleansing with cold water and soap water ; and as brine, cactus, moss, soybean paste, oil, etc. The cleansing with Korean gin, cold water, soap water, brine, vinegar is based upon cleaning and sterilizing functions ; and the use of cucumber is based upon nu. trition provision, and strengthening of resisting power by adjustment of metabolism. The use of potato, cactus, moss, oil and eggs is said to be effective, but their phamacological functions are not clarified. In view of the above results, we can realize that the drugs that have been employed in herb remedies are quite diverse. However, in regard to majority of herb remedies that have been employed by symptoms, the pharmacological functions of their drugs have not been clarified, and they are merely known as effective. Furthermore, they have not been recorded in the literature as yet ; and we confirm that there have been many herb remedies that were executed without the proper knowlege of their effects. It is now our view that the results of this survey may be utilized for consulting data in regard to the use of herb remedies.

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영농방법에 따 른 시기별 미꾸리 개체군의 전장-체중 분석 (Monthly Change of the Length-weight Relationship of the Loach (Misgurnus anguillicaudatus) Population in Paddy Fields by Farming Practices)

  • 신현선;송영주;권순익;어진우;이승현;김명현
    • 환경생물
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    • 제36권1호
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    • pp.1-10
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    • 2018
  • 논 생태계는 일부 생물의 개체군 유지를 위한 중요한 서식지로서 역할을 하고 있다. 이러한 논 생태계를 대상으로 농법에 따른 수생태계에 미치는 영향에 대한 연구는 미비하다. 따라서 친환경농법 및 관행농법과 같은 재배 방식의 차이는 생물서식지에 다양한 영향을 미치기 때문에 두 농법에 따른 미꾸리 개체군의 서식특성 및 성장도의 차이를 확인하였다. 조사기간은 2015년 5월부터 9월까지였고, 대호 간척농지를 대상지로 하였다. 영농방법에 따라 개체수, 미꾸리 개체군 분포, 전장-체중 상관도 및 비만도 지수 비교, 미꾸리 개체군의 전장크기 등을 비교하였다. 미꾸리 개체군의 크기는 친환경 논에서 월등히 큰 것을 확인할 수 있었다. 또한 연령대 분포는 친환경 논이 다양한 연령층의 미꾸리가 서식하는 것으로 나타났으나 관행 논은 Age $0^+$ (28~51 mm)의 개체들이 대부분 성장하지 못하고 사멸하며, 일부 내성을 지니는 개체만이 성장하는 것으로 판단된다. 특히 5월에 관행 논과 친환경 논에서 거의 비슷한 미꾸리 개체수를 보이는 것은 로터리 및 경운에 의해서 토양의 얕은 깊이에 서식하는 미꾸리가 폐사된 것으로 판단되어 무경운이 미꾸리 개체군 유지에 큰 기여를 할 것으로 판단된다. 친환경 논의 미꾸리는 먹이섭식 및 영양상태가 안정적인 것으로 나타났다. 또한 6월에 관행 논 및 친환경 논에서 성장도 및 비만도가 낮은 것에 대하여는 추가적인 조사연구가 필요할 것으로 판단된다. 관행 논에서 미꾸리 개체군의 개체수 회복 및 연령대 회복을 위해서는 농약과 같은 화학물질 사용을 제한해야할 것으로 사료되며, 또한 관행농법보다 친환경농법으로 재배할 경우 미꾸리 개체의 유지 및 회복에 큰 기여를 할 것으로 기대된다.