• Title/Summary/Keyword: livestock species

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Application of molecular biology methods to identify species of animal bones excavated from archaeological sites: focusing on the case of Bonghwang-dong, Gimhae

  • Suyeon, Kim;Eun Min, Cho;Yun-Ji, Kim;So Jin, Kim
    • 농업과학연구
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    • 제48권4호
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    • pp.927-933
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    • 2021
  • People have lived with and used animals for various purposes since the Paleolithic age. Therefore, animal bone research is interesting because it can infer the status of use, determine species, and ascertain the uses of animals that lived at the time. An analysis of ancient DNA was attempted to identify the species of ancient animal bones excavated from an archaeological site. Twelve animal bones from the Geumgwan Gaya period, excavated in Bonghwang-dong, Gimhae, were used in this study. After extracting DNA from the sample, polymerase chain reaction (PCR) gene amplification was performed. Species-specific primers of livestock groups such as pig, cattle, and deer were selected and used. This livestock group was a major source of protein for people who lived on the Korean Peninsula at that time. As a result, 11 sample species were identified. This study is contributes to the restoration of past life information by applying biological technologies to archaeological sites. It is also expected that such analyses of biological remains will ultimately be used to restore historical and cultural information.

Evaluation for Impacts of Nitrogen Source to Groundwater Quality in Livestock Farming Area

  • Lee, Gyeong-Mi;Park, Sunhwa;Kim, Ki-In;Jeon, Sang-Ho;Song, Dahee;Kim, Deok-hyun;Kim, Tae-Seung;Yun, Seong-Taek;Chung, Hyen Mi;Kim, Hyun-Koo
    • 한국토양비료학회지
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    • 제50권5호
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    • pp.345-356
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    • 2017
  • We investigated 52 livestock farming complexes in Gyeong-Gi and Incheon provinces based on low, medium, and high livestock density and groundwater quality. The objective of this study was to evaluate a relationship between nitrate N concentration in groundwater and animal factors, such as livestock density and animal species. 2,200 groundwater samples for 3 years from 2012 to 2014 at Gyeong-Gi and Incheon provinces were collected and analyzed for pH, EC, DO, ORP, temperature, major anions and cations, such as $NO_3-N$, ${HCO_3}^-$, ${PO_4}^-$, ${SO_4}^{2-}$, $Cl^-$, $NH_4-N$, $K^+$, $Na^+$, $Ca^{2+}$, $Mg^{2+}$, T-N, and TOC. Average concentration of total N for generated load density was $23,973g\;day^{-1}\;km^{-2}$ for cattle, $51,551g\;day^{-1}\;km^{-2}$ for pig, and $52,100g\;day^{-1}\;km^{-2}$ for poultry. For animal feeding species, average ratio for generated load over discharge load was 16.1% for cattle, 7.8% for pig, and 7.1% for poultry. Therefore, cattle feeding region is highly vulnerable for water pollution compared to pig and poultry feeding areas. The concentrations of chloride, nitrate, and total N in the groundwater samples were higher at high animal farming regions than other regions. The average concentration of nitrate, and chloride in groundwater samples was $5.0mg\;L^{-1}$, $16.6mg\;L^{-1}$ for low livestock density, $6.9mg\;L^{-1}$, $17.7mg\;L^{-1}$ for medium livestock density and $7.6mg\;L^{-1}$, $22.7mg\;L^{-1}$ for high livestock density and total nitrogen (T-N) was $7.7mg\;L^{-1}$ for low livestock density, $9.4mg\;L^{-1}$ for medium livestock density, $10.7mg\;L^{-1}$ for high livestock density. In conclusion, based on this research, for managing groundwater quality near livestock farming regions, $Ca-(Cl+NO_3)$ group from the Piper diagram is more efficient than using 19 factors for water quality standard.

Evaluation of Different Yeast Species for Improving In vitro Fermentation of Cereal Straws

  • Wang, Zuo;He, Zhixiong;Beauchemin, Karen A.;Tang, Shaoxun;Zhou, Chuanshe;Han, Xuefeng;Wang, Min;Kang, Jinhe;Odongo, Nicholas E.;Tan, Zhiliang
    • Asian-Australasian Journal of Animal Sciences
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    • 제29권2호
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    • pp.230-240
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    • 2016
  • Information on the effects of different yeast species on ruminal fermentation is limited. This experiment was conducted in a $3{\times}4$ factorial arrangement to explore and compare the effects of addition of three different live yeast species (Candida utilis 1314, Saccharomyces cerevisiae 1355, and Candida tropicalis 1254) at four doses (0, $0.25{\times}10^7$, $0.50{\times}10^7$, and $0.75{\times}10^7$ colony-forming unit [cfu]) on in vitro gas production kinetics, fiber degradation, methane production and ruminal fermentation characteristics of maize stover, and rice straw by mixed rumen microorganisms in dairy cows. The maximum gas production (Vf), dry matter disappearance (IVDMD), neutral detergent fiber disappearance (IVNDFD), and methane production in C. utilis group were less (p<0.01) than other two live yeast supplemented groups. The inclusion of S. cerevisiae reduced (p<0.01) the concentrations of ammonia nitrogen ($NH_3$-N), isobutyrate, and isovalerate compared to the other two yeast groups. C. tropicalis addition generally enhanced (p<0.05) IVDMD and IVNDFD. The $NH_3$-N concentration and $CH_4$ production were increased (p<0.05) by the addition of S. cerevisiae and C. tropicalis compared with the control. Supplementation of three yeast species decreased (p<0.05) or numerically decreased the ratio of acetate to propionate. The current results indicate that C. tropicalis is more preferred as yeast culture supplements, and its optimal dose should be $0.25{\times}10^7$ cfu/500 mg substrates in vitro.

국내 유통 식육 및 식육가공품에서 축종감별을 위한 PCR 및 ELISA 검사법 검증 (Validation of PCR and ELISA Test Kits for Identification of Domestic Animal Species in Raw Meat and Meat Products in Korea)

  • 허은정;고은경;서건호;김영조;박현정;위성환;문진산
    • 한국식품위생안전성학회지
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    • 제29권2호
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    • pp.158-163
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    • 2014
  • 본 연구에서는 상용화 되고 있는 PCR 및 ELISA kit를 사용하여 국내에서 유통되고 있는 소, 돼지, 닭, 오리, 칠면조, 염소, 양, 말 등 8종의 식육, 혼합육, 그리고 식육가공품에 대하여 축종 감별능력을 평가하였다. 신선육에 대한 RAW meat ELISA kit$^{(R)}$의 검출한계는 축종별 함유율 0.20%~0.05% 이었고, 열처리 혼합육에서는 열처리 온도 및 시간, 그리고 축종별로 검출한계는 함유율 1.0%~0.05% 이하까지 다양한 차이를 나타내었다. 8종의 식육에 대한 축종별 감별력은 소 94.5%, 돼지 93.3%, 양 90.0%, 오리, 염소, 말, 칠면조 모두에서 100%를 나타내었다. Powercheck Animal Species ID PCR kit$^{TM}$의 경우에는 함유율 0.05%의 검출한계를 나타내었고 8종의 모든 축종에서 100%의 특이도를 나타내어 축종별 감별력이 우수한 것으로 나타났다. 또한, 햄, 소시지, 분쇄가공품, 식육추출가공품 등 총 60개 식육가공품에 대한 Cooked meat ELISA kit$^{(R)}$의 감별력은 햄(35.3%), 소시지(13.6%), 분쇄가공육(12.5%)의 순으로 나타났으며, 2종 이상의 혼합육에서는 상대적으로 낮은 감별력을 보여 제조과정에서 식육간 교차오염에 의한 혼입가능성이 있는 것으로 확인되었다. 쇠고기 육포 54개 제품에 대하여 다른 고기 혼입여부를 PCR Kit로 검사한 결과 13개 제품에서 돼지고기 유전자가 검출되었지만 ELISA Kit에서는 모두 음성으로 나타났다. PCR 양성 시료의 제조공정 중 교차오염 여부를 조사한 결과, 텀블러, 채반, 절단기, 건조기가 쇠고기 및 돼지고기 육포 생산라인에 동일하게 사용되어 교차오염에 의한 혼입으로 추정되었다. 종교적 이유 및 일부 특정 육류에 대한 알러지 반응 등 식품안전 확보차원에서 제품의 원재료의 올바른 표시와 식육간 교차오염이 발생되지 않도록 철저한 품질관리가 되어야 할 것으로 판단된다.

Molecular Sexing and Species Identification of the Processed Meat and Sausages of Horse, Cattle and Pig

  • Kim, Yoo-Kyung;Kang, Yong-Jun;Kang, Geun-Ho;Seong, Pil-Nam;Kim, Jin-Hyoung;Park, Beom-Young;Cho, Sang-Rae;Jeong, Dong Kee;Oh, Hong-Shik;Cho, In-Cheol;Han, Sang-Hyun
    • 한국수정란이식학회지
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    • 제31권1호
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    • pp.61-64
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    • 2016
  • We developed a polymerase chain reaction (PCR)-based molecular method for sexing and identification using sexual dimorphism between the Zinc Finger-X and -Y (ZFX-ZFY) gene and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for mitochondrial DNA (mtDNA) cytochrome B (CYTB) gene in meat pieces and commercial sausages from animals of different origins. Sexual dimorphism based on the presence or absence of SINE-like sequence between ZFX and ZFY genes showed distinguishable band patterns between male and female DNA samples and were easily detected by PCR analyses. Male DNA had two PCR products appearing as distinct two bands (ZFX and ZFY), and female DNA had a single band (ZFX). Molecular identification was carried out using PCR-RFLP of CYTB gene, and showed clear species classification results. The results yielded identical information on the sexes and the species of the meat samples collected from providers without any records. The analyses for DNA isolated from commercial sausage showed that pig was the major source but several sausages originated from chicken and Atlantic cod. Applying this PCR-based molecular method was useful and yielded clear sex information and identified the species of various tissue samples originating from livestock.

THE IETS STATISTICS OF EMBRYO TRANSFERS IN LIVESTOCK IN THE WORLD FOR THE YEAR 1999; A NEW RECORD FOR BOVINE VIVO-DERIVED EMBRYOS TRANSFERRED

  • Thibier, Michel
    • 한국수정란이식학회:학술대회논문집
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    • 한국수정란이식학회 2001년도 발생공학 국세심포지엄
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    • pp.25-34
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    • 2001
  • The Committee has, for the 9th consecutive year, compiled statistics of embryos collected and transferred worldwide. Geographically, all regions have participated in the survey; however, there are still some places where it has been impossible to retrieve such data (particularly in Asia). The statistics, therefore, are partially underestimated. By contrast, in other areas, the system has proven to be more efficient than before, particularly in North and South America. This has resulted in the present report, which gives a somewhat more satisfactory picture of the current situation of the ET industry. For the second year, it has also been possible to collect data for several species other than cattle. In cattle, the number of in vivo-derived embryos collected and transferred has once again increased with more than half a million embryos trans-ferred in 1999(520,712), a new record. The number of bovine in vitro-produced embryos has remained stable this year as compared with the previous year(approximately 30,000 embryos transferred). However, there are still teams that have not Yet reported their data. More than 10,000 embryos in each of the ovine and caprine species and close to 2,000 cervid embryos were reported transferred in 1999. Some 500 embryos of the equine species and a few thousand in the porcine and rabbit species have also been transferred in 1999. It is concluded that the ET industry continues to be very active, and, in many species, it encompasses a larger segment of the livestock population overall, which is to the farmer s benefit.

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임간 초지 개발에 관한 연구 VI. 차광정도에 따른 주요목초의 지상부 및 지하부의 생육형질 변이와 그 상관관계 (Studies on the Grassland Development in the Forest VI. Effect of shading degrees on the correlations and the variations of agronomic characteristics in the shoot and root part of some grases)

  • 박문수;서성;한영춘;류종원
    • 한국초지조사료학회지
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    • 제7권2호
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    • pp.79-86
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    • 1987
  • This study was carried out to investigate the relationships and the variations of agronomic characteristics in the shoot and root part of grasses for the basic data of the development, the management and the utilization of pasture in the forest. This study was conducted with 0% (full sunlight), 25%, 50% and 75% of shading degrees after orchardgrass, timothy, perennial ryegrass, ladino clover and orchardgrass dominated mixture were planted on Aug. 25 in 1984. The results are summarized as follows: 1. Dry matter yield in 25% of shading was increased 3-1 7% compared with 0% (full sunlight), while 50 and 75% of shading were decreased 28-35% and 44-60%, respectively. Dry matter yield of timothy, perennial ryegrass and ladino clover were decreased 10, 8 and 33%, respectively, compared with orchardgrass, but there was little difference between orchard grass and mixture. 2. Root distribution by different shading degrees was the maximum at 25%, followed by 0, 50 and 75% of shading degrees. Root distribution among different species was the maximum at orchardgrass, followed by perennial ryegrass and timothy. Especially, root distribution of timothy was very little compared with different species. And significantly positive correlation was observed in the relationship between root weight and dry matter yield (r=0.8 102**). 3. The physiological activity of root by different shading degrees was declined by more shading. 4. The length and the percentage of blade by different shading degrees was an increase tendency by more shading. The length and the percentage of blade among different species was followed by timothy, perennial ryegrass and orchardgrass, in that order. And the correlation between the percentage of blade and dry matter yield was significantly negative.

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Equine helminths: prevalence and associated risk factors in Gamo Gofa Zone, Ethiopia

  • Yared Abate Getahun;Bekahegn Simeon Tsalke;Abreham Wondimu Buzuneh;Mekoya Mereta Mejo;Wondyfraw Tsegaw Habtewold
    • Journal of Veterinary Science
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    • 제25권3호
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    • pp.41.1-41.12
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    • 2024
  • Importance: Equines are indispensable in reducing the huge burden on children and women and income generation. On the other hand, minimal attention is given to improving their health and welfare. Objective: This study examined the prevalence and associated risk factors of helminth parasites of equine in the Gamo Gofa Zone. Methods: A cross-sectional study was employed from June 2019 to March 2020. The study districts and Kebeles were selected purposively based on agroecology whereas selection of study households and animals were performed based on simple random sampling techniques. Identification of nematode, trematode parasite ova and larvae of D. arnfieldi were done by floatation, sedimentation, and Baermann techniques respectively. Descriptive statistics and logistic regression was applied to estimate the prevalence and association of risk factors with helminth parasites. Results: The overall helminth parasite prevalence in the study area was 90.4%, 425/470 (95% [CI], 87.16-92.9). The prevalence of Strongyle, Fasciola, O. equi, P. equorum, D. arnfieldi, and mixed parasite infections were 65.1%, 21.7%, 17.4%, 34%, 34%, and 58.1%, respectively. Infections from Fasciola species and D. arnfieldi infection were four ([AOR], 4.4; 95% CI, 2-9.4) and two times (AOR, 2; 95% CI, 1.1-3.6) respectively more likely occur in donkeys than in mules. The occurrence of Strongyle species in midland agroecology was two times (AOR, 2.6; 95% CI, 1.4-4.7) more likely than lowland agroecology. Conclusions and Relevance: The present study identified diverse species of equine helminth parasites that necessitate urgent disease control and prevention measures.

가축에서 테트라사이클린 내성 장구균 조사 및 분자생물학적 특성규명 (Prevalence and molecular characterization of tetracycline-resistant Enterococcus isolates from livestock)

  • 김철민;강수진;이병종;이성재;육대수
    • 한국동물위생학회지
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    • 제33권2호
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    • pp.143-149
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    • 2010
  • BIn the present study, Enterococcus isolates originating from livestock were studied for the phenotypic and genotypic assessment of tetracycline resistance. A total of 74 isolates encompassing the species Enterococcus faecalis (n=12) and E. faecium (n=62) displayed phenotypic resistance to tetracycline. Tetracycline resistance gene [tet (M), 1,886bp] were sequenced by dye terminator cycle sequencing method and compared with tet (M) sequences available from the GenBank database. Sequencing analysis of PCR amplicons showed high homology to the reference strains ranging 97.2~100%. The tet (M) genes were divided into three major subgroups according to phylogenetic analysis. The genetic information obtained from this study could be useful for the molecular study of enterococci.

임간 초지 개발에 관한 연구 VII. 차광정도가 주요목초의 개체군생장향, 순동화율 및 광합성능력에 미치는 영향 (Studies on the Grassland Development in the Frest VII. Effect of shading degrees on the crop growth rate, net assimilation rate and photosynthtic active of main grasses)

  • 박문수;서성;한영춘;류종원;이종열
    • 한국초지조사료학회지
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    • 제7권3호
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    • pp.122-127
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    • 1987
  • This experiment was carried out to investigate the effect of shading degrees (0:full sunlight, 25, 50 and 75%) on the crop growth rate (CGR), net assimilation rate (NAR) and photosynthetic activity of grasses for obtaining the basic data on the development, management and utilization of pasture in the forest. For the test, different artificial shading houses were established and pasture species used in this study were orchardgrass, timothy, perennial ryegrass and ladino clover. The experiment was performed at Livestock Experiment Station, Suwon, in 1984-'85. The results obtained are summarized as follows. 1. The highest CGR was obtained at 0% (full sunlight) and 25% of shading, followed by 50 and 75% of shading degrees, in that order. CGR of orchardgrass and perennial ryegrass were higher than those of timothy and ladino clover. 2. NAR was significantly decreased as the shading degrees increased. The highest NAR was observed at perennial ryegrass plot. 3. The photosynthetic activity during summer season was the highest at 25% of shading, followed by 50, 0 and 75% of shading degrees. While, the photosynthetic activity during fall season was the highest at 25% of shading, followed by 50, 0 and 75% of shading degrees, in that order. Those of orchardgrass and perennial ryegrass were remarkably higher than that of timothy. 4. A significant positive correlation was obtained in the relationship between CGR and NAR of main grasses, regardless of pasture species and investigated date.

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