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http://dx.doi.org/10.7744/kjoas.20210078

Application of molecular biology methods to identify species of animal bones excavated from archaeological sites: focusing on the case of Bonghwang-dong, Gimhae  

Suyeon, Kim (Conservation Science Division, National Research Institute of Cultural Heritage)
Eun Min, Cho (Conservation Science Division, National Research Institute of Cultural Heritage)
Yun-Ji, Kim (Conservation Science Division, National Research Institute of Cultural Heritage)
So Jin, Kim (Conservation Science Division, National Research Institute of Cultural Heritage)
Publication Information
Korean Journal of Agricultural Science / v.48, no.4, 2021 , pp. 927-933 More about this Journal
Abstract
People have lived with and used animals for various purposes since the Paleolithic age. Therefore, animal bone research is interesting because it can infer the status of use, determine species, and ascertain the uses of animals that lived at the time. An analysis of ancient DNA was attempted to identify the species of ancient animal bones excavated from an archaeological site. Twelve animal bones from the Geumgwan Gaya period, excavated in Bonghwang-dong, Gimhae, were used in this study. After extracting DNA from the sample, polymerase chain reaction (PCR) gene amplification was performed. Species-specific primers of livestock groups such as pig, cattle, and deer were selected and used. This livestock group was a major source of protein for people who lived on the Korean Peninsula at that time. As a result, 11 sample species were identified. This study is contributes to the restoration of past life information by applying biological technologies to archaeological sites. It is also expected that such analyses of biological remains will ultimately be used to restore historical and cultural information.
Keywords
ancient animal bones; ancient DNA (aDNA); archaeological site; species identification;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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1 Cho TS. 2017. A study of use of animals by Kwakji people, in Jeju island. The Journal of the Humanities 110:105-131. [in Korean]   DOI
2 Fickel J, Hohmann U. 2006. A methodological approach for non-invasive sampling for population size estimates in wild boars (Sus scrofa). European Journal of Wildlife Research 52:28-33.   DOI
3 Gaya National Research Institute of Cultural Heritage. 2018. Leaflet. Accessed in https://www.nrich.go.kr/kor/resource/file/gaya_leaflet.pdf on 1 October 2021. [in Korean]
4 Kang MC, Han SH, Jung YH, Oh JH, Kim GO, Ko JW, Oh MY. 2007. Genetic analysis of ancient bone of Cervidae animals from archaeological site in Jeju, Korea. Integrative Biosciences 11:147-153.   DOI
5 Kang S, Cho EM, Kim SH, Kim YJ, Lee J. 2014. The application of a chemical assessment of archaeological animal bone by Fourier transform infrared spectroscopy and x-ray diffraction. Journal of Analytical Science and Technology 27:300-307. [in Korean]   DOI
6 Kim D, Stoyakin M. 2019. Current status and analysis of animal bones from Palhae archaeological sites. Journal of Association of Koguryo Balhae 65:239-277. [in Korean]
7 Kim HS. 2020. On the usage and discarding of bear (Ursus sp) bones excavated from Wolseong moat site. Journal of Central Institute of Cultural Heritage 31:77-100. [in Korean]   DOI
8 Kim JH, Han SH, Kang MC, Oh JH, Jung YH, Kim GO, Oh MY. 2006. Ancient pigs on Jeju island, Korea: Molecular identification and phylogenetic relationship with extant native pigs. Korean Journal of Genetics 28:385-393.
9 Kim M, Lee SI, Park H, Lee SD. 2020. Ecological studies of wild boars (Sus scrofa) in Yeongwol Hanbando wetland inferred through DNA analysis of non-invasive samples. Journal of Environmental Impact Assessment 29:230-238. [in Korean]   DOI
10 Kwon KD, Lee A, Lim J, Cho S, Lee W, Cho BK, Seo Y. 2020. Quality grading of Hanwoo (Korean native cattle breed) subimages using convolutional neural network. Korean Journal of Agricultural Science 47:1109-1122.   DOI
11 Lee JJ. 2012. Archaeology and Agriculture. pp. 71-89. The Korean Archaeological Society Publishing, Daejeon, Korea. [in Korean]
12 Lee SH, Park BH, Sharma A, Dang CG, Lee SS, Choi TJ, Choy YH, Kim HC, Jeon KJ, Kim SD, et al. 2014. Hanwoo cattle: Origin, domestication, breeding strategies and genomic selection. Journal of Animal Science and Technology 56:2.
13 Yang DY, Eng B, Waye JS, Dudar JC, Saunders SR. 1998. Technical note: Improved DNA extraction from ancient bones using silica-based spin columns. American Journal of Physical Anthropology 105:539-543.   DOI
14 Park YC, Ahn CY, Jin SO, Lim JY, Kim KH, Lee JH, Cho TY, Lee HJ, Park KS, Yoon HS. 2012. Identification of raw materials in processed meat products by PCR using species-specific primer. Journal of Food Hygiene and Safety 27:68-73. [in Korean]   DOI
15 Surovell TA, Stiner MC. 2001. Standardizing infra-red measures of bone mineral crystallinity: An experimental approach. Journal of Archaeological Science 28:633-642.   DOI