• Analytical Science and Technology
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• v.32 no.4
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• pp.139-146
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• 2019

A simple and reliable analytical method based on high-performance liquid chromatography-ultraviolet detection was established for the analysis of the flowers of Chrysanthemum morifolium (CM). Luteolin-7-O-glucoside (LU7G) was chosen as a target analyte considering its content, availability, and ease of analysis. Chromatographic separation of LU7G was achieved using a Phenomenex Gemini $C_{18}$ column ($250{\times}4.6mm$, $5{\mu}m$) run with a mobile phase consisting of 0.5 % acetic acid in water and 0.5 % acetic acid in acetonitrile at a flow rate of $1.0mL\;min^{-1}$. The detection wavelength and column temperature were set at 350 nm and $40^{\circ}C$, respectively. Method validation was performed according to the AOAC guidelines and the method was specific, linear ($R^2=0.9991$ for $50-300{\mu}g\;mL^{-1}$), precise (${\leq}3.91%$RSD), and accurate (100.1-105.7 %). The limits of detection and quantification were 3.62 and $10.96{\mu}g\;mL^{-1}$, respectively. The established method was successfully applied to determine the contents of LU7G in various batches of bulk CM extracts and labscale CM extract. The developed method is a readily applicable method for the quality assessment of CM and its related products.

• Mass Spectrometry Letters
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• v.10 no.1
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• pp.18-26
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• 2019

We developed a bioanalytical method for simultaneous determination of nine NBOMe derivatives (25H-NBOMe, 25B-NBOMe, 25E-NBOMe, 25N-NBOMe, 25C-NBOH, 25I-NBOH, 25B-NBF, 25C-NBF, and 25I-NBF) in human plasma using liquid chromatography tandem mass spectrometry (LC-MS/MS). Human plasma samples were pre-treated using solid-phase extraction. Separation was achieved on a C18 column under gradient elution using a mobile phase containing 0.1% formic acid in acetonitrile and 0.1% formic acid in water at a flow rate of 0.3 mL/min. Mass detection was performed in the positive ion mode using multiple reaction monitoring. The calibration range was 1-100 ng/mL for all quantitative analytes, with a correlation coefficient greater than 0.99. The intra- and inter-day precision and accuracy varied from 0.85 to 6.92% and from 90.19 to 108.69%, respectively. The recovery ranged from 86.36 to 118.52%, and the matrix effects ranged from 27.09 to 99.72%. The stability was acceptable in various conditions. The LC-MS/MS method was validated for linearity, accuracy, precision, matrix effects, recovery and stability in accordance with the FDA guidance. The proposed method is suitable for reliable and robust routine screening and analysis of nine NBOMe derivatives in forensic field.

• Tribology and Lubricants
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• v.37 no.2
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• pp.71-76
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• 2021

As opposed to using fossil fuels, we need to use eco-friendly resources such as sunlight, raindrops and wind to produce electricity and combat environmental pollution. A triboelectric nanogenerator (TENG) is a device that converts mechanical energy into electricity by inducing repetitive contact and separation of two dissimilar materials. During the contact and separation processes, electron flow occurs owing to a change in electric potential of the contacting surface caused by contact electrification and electrostatic induction mechanisms. A solid-solid contact TENG is widely known, but it is possible to generate electricity via liquid-solid contact. Therefore, by designing a hydrophobic TENG, we can gather electricity from raindrop energy in a feasible manner. To fabricate the superhydrophobic surface of TENGs, we employ a dip coating technique to synthesize an octadecylamine (ODA)- and polydimethylsiloxane (PDMS)-based coating on polyethylene terephthalate (PET). The synthesized coating exhibits superhydrophobicity with a contact angle greater than 150° and generates a current of 2.2 ㎂/L while water droplets fall onto it continuously. Hence, we prepare a box-type TENG, with the ODA/PDMS coating deposited on the inside, and place a 1.5 mL water droplet into it. Resultantly, we confirm that the induced vibration causes continuous impacts between the ODA/PDMS coating and the water, generating approximately 100 pA for each impact.

• Journal of the Korean Society of Propulsion Engineers
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• v.24 no.5
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• pp.21-33
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• 2020

In this study, the program for system analysis of an expander cycle rocket engine using liquid hydrogen as a fuel was developed. The properties of hydrogen were considered by the ratio of isomers with temperature. The analysis procedure was established with the open and closed types of the expander cycle engine and the simulation methods were suggested for each component. To validation of the analysis program, we compared the performance of the engine operating point and the analysis results performed overseas for Vinci and SE-21D, which are expander cycle engines. As a result of the analysis, the main performance factors of the system, such as the mass flow of the propellant, specific thrust, and power, except for some of the inaccurate input information, showed high accuracy with an error of around 1-2%.

• Nuclear Engineering and Technology
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• v.54 no.2
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• pp.589-600
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• 2022

Investigations on the molten-pool sloshing behavior are of essential value for improving nuclear safety evaluation of Core Disruptive Accidents (CDA) that would be possibly encountered for Sodium-cooled Fast Reactors (SFR). This paper is aimed at synthesizing the knowledge from our recent studies on molten-pool sloshing behavior with solid particles conducted at the Sun Yat-sen University. To better visualize and clarify the mechanism and characteristics of sloshing induced by local Fuel-Coolant Interaction (FCI), experiments were performed with various parameters by injecting nitrogen gas into a 2-dimensional liquid pool with accumulated solid particles. It was confirmed that under different particle-bed conditions, three representative flow regimes (i.e. the bubble-impulsion dominant, transitional and bed-inertia dominant regimes) are identifiable. Aimed at predicting the regime transitions during sloshing process, a predictive empirical model along with a regime map was proposed on the basis of experiments using single-sized spherical solid particles, and then was extended for covering more complex particle conditions (e.g. non-spherical, mixed-sized and mixed-density spherical particle conditions). To obtain more comprehensive understandings and verify the applicability and reliability of the predictive model under more realistic conditions (e.g. large-scale 3-dimensional condition), further experimental and modeling studies are also being prepared under other more complicated actual conditions.

• Journal of the Korean Geotechnical Society
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• v.22 no.1
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• pp.45-52
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• 2006

A theoretical equation, from which we can get a suitable ratio of bentonite at bentonite-mixed soil, was derived for desigri of the impermeable condition. Bentonite is a soil with great expansion property and it has the permeability lower than $1\times10^{-7}cm/sec$ in spite of its maximum expansion state. Accordingly if the void of soil is filled with the liquid of bentonite, water will flow only through the veid of bentonite liquid. And the permeability of bentonite-mixed soil will always satisfy the condition as impermeable zone. However, because it is very difficult to mix uniformly bentonite with soil, it is thought that the actual mixing ratio fur the impermeable zone will be grater than that by theoretical equation. Permeability tests were performed to check the equation and a modified equation was suggested from the experimental results.

• Analytical Science and Technology
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• v.37 no.2
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• pp.98-113
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• 2024

The current investigation entails the characterization of five degradation products (DPs) formed under different stress conditions of loteprednol using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, this study developed a stable high-performance liquid chromatography (HPLC) method for evaluating loteprednol along with impurities. The method conditions were meticulously fine-tuned which involved the exploration of the appropriate solvent, pH, flow of the mobile phase, columns, and wavelength. The method conditions were carefully chosen to successfully resolve the impurities of loteprednol and were employed in subsequent validation procedures. The stability profile of loteprednol was exposed to stress degradation experiments conducted under five conditions, and DPs were structurally characterized by employing LC-MS/MS. The chromatographic resolution of loteprednol and its impurities along with DPs was effectively achieved using a Phenomenex Luna 250 mm C18 column using 0.1 % phosphoric acid, methanol, and acetonitrile in 45:25:30 (v/v) pumped isocratically at 0.8 mL/min with 243 nm wavelength. The method produces an accurate fit calibration curve in 50-300 ㎍/mL for loteprednol and LOQ (0.05 ㎍/mL) - 0.30 ㎍/mL for its impurities with acceptable precision, accuracy, and recovery. The stress-induced degradation study revealed the degradation of loteprednol under basic, acidic, and photolytic conditions, resulting in the formation of seven distinct DPs. The efficacy of this method was validated through LC-MS/MS, which allowed for the verification of the chemical structures of the newly generated DPs of loteprednol. This method was appropriate for assessing the impurities of loteprednol and can also be appropriate for structural and quantitative assessment of its degradation products.

• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.4
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• pp.90-102
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• 2024

Haploid/double haploid plants developed from isolated microspores can significantly accelerate plant breeding. Haploid plants can naturally double their chromosomes to create a pure homozygous line of diploid plants. We present a method for producing embryos from isolated microspores of hot peppers (Capsicum annuumL.). We analyzed the polyploidization levels of the regenerated plants. The donor plants produced the optimal stage of microspores following short-term growth under low-intensity light, which resulted in high rates of embryogenesis and cotyledonary embryogenesis. To find an efficient culture method, liquid, doubled-layer, and 2-step cultures were tested. Liquid culture yielded the highest number of embryos, whereas the highest efficiency for cotyledonary embryogenesis was afforded by the doubled-layer culture. When normal cotyledonary embryos were transplanted onto a regeneration medium, they developed into complete plants. From these, 208 plants were tested via flow cytometric analysis, and 35.6% and 72.7% of the chromosomes from the Milyang-jare and LV2319 genotypes, respectively, were found to be spontaneous double haploids. These results are the same as those obtained on analyzing horticultural characteristics, including the size of leaves and the size and shape of fruits. The present study provides information on the practical application of isolated microspore culture of hot peppers, factors that affect embryogenesis, and methods for polyploidy testing.

• FOCUS: LIFE SCIENCE
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• no.1
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• pp.1.1-1.4
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• 2024

This article provides comprehensive guidance on the maintenance, cleaning, regeneration, and storage of silica-based HPLC (High-Performance Liquid Chromatography) columns. The general considerations emphasize the importance of using in-line filters and guard cartridges to protect columns from blockage and irreversible sample adsorption. While these measures help, contamination by strongly adsorbed sample components can still occur over time, leading to an increase in back pressure, loss of efficiency, and other issues. To maximize column lifetime, especially with UHPLC (Ultra-High Performance Liquid Chromatography) columns, it is advisable to use ultra-pure solvents, freshly prepared aqueous mobile phases, and to filter all samples, standards, and mobile phases. Additionally, an in-line filter system and sample clean-up on dirty samples are recommended. However, in cases of irreversible compound adsorption or column voiding, regeneration may not be possible. The document also provides specific recommendations for column cleaning procedures, including the flushing procedures for various types of columns such as reversed phase, unbonded silica, bonded normal phase, anion exchange, cation exchange, and size exclusion columns for proteins. The flushing procedures involve using specific solvents in a series to clean and regenerate the columns. It is emphasized that the flow rate during flushing should not exceed the specified limit for the particular column, and the last solvent used should be compatible with the mobile phase. Furthermore, the article outlines the storage conditions for silica based HPLC columns, highlighting the impact of storage conditions on the column's lifetime. It is recommended to flush all buffers, salts, and ion-pairing reagents from the column before storage. The storage solvent should ideally match the one used in the initial column test chromatogram provided by the manufacturer, and column end plugs should be fitted to prevent solvent evaporation and drying out of the packing bed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.6
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• pp.729-736
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• 2016

It is necessary to recycle and reuse waste paper for preservation of the global environment. Instead of a solid deinking agent, liquid deinking agents have been made using various fatty acids with EO/PO added for higher deinking effect. The removal efficiency of ink on waste paper was evaluated by the ERIC and brightness properties after processes of recycling pulping (sample composition ONP: OMG = 5:5, concentration 15%, temperature $45^{\circ}C$, pulping time 20 min.) and flotation (concentration 1%, temperature $45^{\circ}C$, air flow 7 L/min, time 8 min.). Also, the ink reattachment of paper was evaluated by hyperwashing. The results show that it is effective for mass-preparation to saponify oleic acid with KOH at $80^{\circ}C$ and to add EO/PO. This deinking agent was used in a paper plant, and we evaluated the brightness, color, and ERIC. The deinking agent showed excellent performance.