• Food Science and Preservation
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• v.11 no.4
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• pp.472-477
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• 2004

This study was carried out to investigate the effect of liquid calcium(LC) on the quality of kimchi during fermentation. LC retarded the decreasing rate of pH and the increasing rate of titratable acidity during fermentation at $10^{\circ}C$, and the effect was more conspicuous at $7.0\%(v/w)$ than at $3.5\%(v/w)$ in each LC type. Microbial counts of total and lactic acid bacteria were lower in LC added kimchi than control before 8 days but higher in LC added kimhi after 8 days. Changes in color of a value were gradually increased, but L and b value were decreased. Calcium content of added LC on kimchi; A concentration $3.5\%(v/w),\;7.0\%(v/w)$ were $103{\sim}110\;mg\%,\;145{\sim}163\;mg\%$ and B concentration $3.5\%,\;7.0\%$ were $140{\sim}151\;mg\%,\;210{\sim}220\;mg\%$.

• Archives of Pharmacal Research
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• v.9 no.4
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• pp.211-214
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• 1986

A simultaneous determination method of alkaline earth metals was attempted with the conventional high performance liquid chromatographic system. Four cations, namely, magnesium, calcium, strontium and barium ion, were injected directly as aqueous solution into an eluent containing copper chloride solution and and were successfully separated and determined on a separating column (Zipax SCX, 4.6 mm i.d. ${\times}25$ cm length, Du Pont, USA) by using a variable wavelength UV detector. The linear calibration curves were obtianed in the range from $1.0{\times}10^{-4}M$ to $5.0{\times}10^{-4}M$ and the correlation coefficient of the calibration curve for each metal of magnesium and calcium in tap water. Alkaline earth metals were determined with the conventional high performance liquid chromatographic system.

• Journal of the Korean Ceramic Society
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• v.22 no.1
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• pp.5-10
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• 1985

The effect of calcium lignosulfonate (CLS) on the early hydration characteristics for clinker minerals was investigated. In the presence of CLS and unsufficient gypsum The hydration of $C_3$A lowered CLS adsorption to form ettrin-gite and the residual CLS in the liquid phase accelerated the solubility of C4AF hydration. As the result unreacted $Fe^{3+}$ in the liquid phase would be precipitated gelatinously on $C_3$ hydrates and the hydration of $C_3$ could be retarded. But by addition of optimum gypsum into the cement with CLS the presence of $Fe^{3+}$ in the liquid phase were lowered and $C_3$ hydration would be normallized.

• Journal of Applied Biological Chemistry
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• v.55 no.4
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• pp.263-266
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• 2012

Isolation of iron and calcium-binding peptides derived from cottonseed meal protein (CMP) hydrolysates was investigated. The degree of hydrolysis of CMP by Flavourzyme was monitored using trinitrobenzenesulfonic acid method and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Enzymatic hydrolysis of CMP for 12 h was sufficient for the preparation of CMP hydrolysates, and the hydrolysates were membrane-filtered under 3 kDa as a molecular weight. The filtered solution was fractionated using Q-Sepharose fast flow, Sephadex G-15, and reversed phase-high performance liquid chromatography for iron and calcium-binding peptides. As a result, F51 fraction was obtained as the best candidate for calcium and iron chelation, and the isolated iron and calcium-binding peptides can be used as functional food additives, similar to iron and calcium supplements.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.3
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• pp.593-597
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• 2004

This study was conducted to investigate the quality characteristics of liquid calcium acetate (LCAs) and solid calcium acetate (SCAs), Br-LCA, Pe-LCA and Ap-LCA as liquid form, Br-SCA, Pe-SCA and Ap-SCA as solid form. Calcium acetate was prepared by reacting of vinegars [brown rice (Br), persimmons (Pe) and apple (Ap)] and ash of black snail in order to obtain natural water soluble calcium resources. The pHs of the vinegars for preparing calcium acetate (CA) were the range of 2.34 ∼ 3.06, and the contents of the ash of black snail which reacted to 100 mL of the vinegars were 20.43∼23.50 g. The yields of solid CAs from 100 mL of the vinegars were 11.02∼13.01 g. The colors of liquid and solid CAs were light yellow in Ap-LCA and Ap-SCA, brown in Br-LCA and Br-SCA, dark brown in Pe-LCA and Pe-SCA. Calcium contents of Br-LCA, Pe-LCA and Ap-LCA were 3.02, 2.06 and 2.30% (w/v), and those of Br-SCA, Pe-SCA and Ap-SCA were 27.15, 16.31 and 19.48% (w/w), respectively. The solubilities of the solid CAs were 36.82 ∼ 39.92% (w/v) in distilled water, 32.05 ∼ 39.04% (w/v) in Soju, 13.12 ∼ 18.65% (w/v) in thick soysauce, 38.35 ∼ 38.90% (w/v) in ionic beverage, 33.47 ∼ 35.58% (w/v) in yoghurt, while the solid CAs formed the curds in soymilk and milk. The sour and bitter taste of the CAs were lower, while the astringent taste, fishy flavor and savory taste were higher than those of standard CA.

• Korean Journal of Food Science and Technology
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• v.41 no.6
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• pp.657-661
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• 2009

Liquid microparticulated seaweed calcium was manufactured via a wet grinding process. Thereafter, different forming agents such as cyclodextrin, gum arabic, and Na-caseinate were added to the liquid calcium, which was then spray-dried to investigate the quality of the powdered calcium treatments. The moisture contents of samples were approximately 2%. It was also determined that the different kinds of forming agents did not affect the spray drying efficiency. In addition, calcium solubility was the highest in a solution of pH 2. In buffer solution and vinegar, the powdered calcium made with gum arabic showed the highest solubility among the treatments. The calcium contents of all the powdered microparticulate seaweed calcium samples were about 28%, and calcium content was not affected by the forming agents. The spray-dried calcium powder made by spray drying with gum arabic had the highest water vapor uptake, whereas the seaweed calcium was stable in terms of water adsorption. The results of SEM observations indicated that a portion of the spray-dried calcium powders were in nano-scale after wet-grinding. Among the treatments, the use of saccharides as a forming agent resulted in the most uniform particle distribution after spray-drying.

• Journal of the Korean Wood Science and Technology
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• v.20 no.4
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• pp.57-64
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• 1992

The unique cell wall micropores of pulp fiber can be utilized as loading site in variety of important practical application which could be the basis of new papermaking technologies. One of these includes the manufature of paper containing higher levels of in situ filler precipitated. Hardwood pulp fiber were first impregnated with the solution of sodium carbonate($Na_2CO_3$). The micropores in cell wall of pulp fibers were filled with the liquid salt solution. The second calcium nitrate($Ca(NO_3)_2$) solution formed an insoluble calcium carbonate($CaCO_3$) precipitate within the cell wall micropores by interacting with the first sodium carbonate solution. The effects of chemical concentration and dryness of pulp fibers on the retention of cell wall micropore loaded filler were investigated. The paper properties of cell wall micropore loaded pulp fibers were compared with those of conventionally loaded and lumen loaded pulp fibers. Also the presense of the fillers within the cell wall micropore was observed by SEM. Increasing the chemical concentration to generate the calcium carbonate increased the retention of filler in cell wall micropore loaded pulp fibers. The particle size distribution of precipitated calcium carbonate ranged from $0.1{\mu}m$ to $80{\mu}m$. But, the average particle size of cell wall micropore loaded calcium carbonate was $4{\mu}m$. The paper made from never dried pulp fibers, the cell wall micropores which were filled with calcium carbonate, had better mechanical and optical properties than those of conventionally loaded or lumen loaded pulp fibers.

• Annals of Laboratory Medicine
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• v.38 no.6
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• pp.518-523
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• 2018

Background: Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method. Methods: Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation ($108,200{\times}g$) and high-speed centrifugation ($10,000{\times}g$ for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods-LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI). Results: When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI. Conclusions: High-speed centrifugation ($10,000{\times}g$ for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.

• Journal of Life Science
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• v.9 no.1
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• pp.45-49
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• 1999

Seaweed alginate was acetylated by activated carbon immobilized Pseudomonas syringae in a fluidized bed, up-flow reactor. The acetylation degree of seaweed alginate was about 30%. Calcium-acetylated seaweed alginate gel bead was made and compared to calcium-seaweed alginate gel bead by the scanning electron microscopy (SEM). Structural difference of two gel beads may results from increased viscosity and decreased affinity of acetylated seaweed alginate for calcium ion. On the basis of interior and exterior structure of calcium-acetylated seaweed alginate gels and property of acetylated seaweed alginate, it seems that acetylated seaweed alginate is used for the supporter for electrophoresis and packing materials for liquid chromatography and gel filtration.

• Journal of Korean Society on Water Environment
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• v.31 no.3
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• pp.253-261
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• 2015

The purpose of this study is the efficient biological treatment of highly concentrated nitrate nitrogen by calcium ion control present within the pickling wastewater. In laboratory scale's experiments research was performed using a sequencing batch reactor and the evaluation of denitrification reaction in accordance with the injection condition of calcium ions, the surface properties of microorganisms, and the evaluation of sludge precipitability were performed. Results of the study showed that the denitrification reaction was delayed when injecting more than 600 mg/L of the calcium ion within the denitrification process. In addition, we observed the absorption form of calcium ions absorbed on the surface of microorganisms following an increase in the calcium ion dose. It was found that as the calcium ion dose increased the sludge precipitability also increased continuously and it is judged that a smooth denitrification induction is possible when treating the nitrate nitrogen by the calcium ion control of pickling waste water and the shortening of precipitation time enables a liquid operation to increase the reaction time.