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http://dx.doi.org/10.3839/jabc.2012.041

Isolation of Iron and Calcium-Binding Peptides from Cottonseed Meal Protein Hydrolysates  

Choi, Dong-Won (Department of Food Science & Technology, Chungnam National University)
Kim, Nam-Ho (Department of Food Science & Technology, Chungnam National University)
Song, Kyung Bin (Department of Food Science & Technology, Chungnam National University)
Publication Information
Journal of Applied Biological Chemistry / v.55, no.4, 2012 , pp. 263-266 More about this Journal
Abstract
Isolation of iron and calcium-binding peptides derived from cottonseed meal protein (CMP) hydrolysates was investigated. The degree of hydrolysis of CMP by Flavourzyme was monitored using trinitrobenzenesulfonic acid method and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Enzymatic hydrolysis of CMP for 12 h was sufficient for the preparation of CMP hydrolysates, and the hydrolysates were membrane-filtered under 3 kDa as a molecular weight. The filtered solution was fractionated using Q-Sepharose fast flow, Sephadex G-15, and reversed phase-high performance liquid chromatography for iron and calcium-binding peptides. As a result, F51 fraction was obtained as the best candidate for calcium and iron chelation, and the isolated iron and calcium-binding peptides can be used as functional food additives, similar to iron and calcium supplements.
Keywords
calcium; cottonseed meal protein; hydrolysates; iron; peptide;
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Times Cited By KSCI : 3  (Citation Analysis)
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