• International Journal of Industrial Entomology and Biomaterials
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• 제22권2호
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• pp.95-100
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• 2011

The effect of diet supplementation of Antheraea pernyi (A. pernyi) silk fibroin on the lipid metabolism and antioxidant defense status in high fat-fed mice was investigated. The animals were given normal control diet (NC group), high fat diet (HF group), or high fat diet supplemented with A. pernyi silk fibroin powder (HFS group) for 7 weeks. After the experimental period, the HF group showed significant increase in body weight, plasma and hepatic total cholesterol levels, and hepatic triglyceride concentration, and decreased activities of hepatic antioxidant enzymes relative to NC group. However, the HFS group exhibited marked reduction in body weight, plasma cholesterol and hepatic triglyceride levels, hepatic lipogenic enzyme activities, and lipid peroxidation rate, and higher high-density lipoprotein (HDL)-cholesterol level, fecal triglyceride content, and antioxidant enzymes activities compared with that of HF group. These findings demonstrate that dietary feeding of A. pernyi silk fibroin could improve the lipid metabolism and antioxidant defense system via regulation of hepatic antioxidant and lipogenic enzymes activities. Hence, this silk fibroin may be beneficial as a functional biomaterial for the development of therapeutic agent against high fat diet-induced hyperlipidemia and its related diseases.

• 한국지역사회생활과학회지
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• 제14권2호
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• pp.41-47
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• 2003

To investigate the effects of hot water soluble extract from Angelicae Gigantis Radix on lipid metabolism and antioxidant defense system in rats fed hypercholesterol diet, Sprague-Dawley male rats were fed either hypercholesterol diet with water or hot water soluble extract from Angelicae Gigantis Radix. Hypercholesterol diet contained 18 % beeftallow and 5 % cholesterol. After 4 weeks of experimental periods serum and liver obtained. The level of serum lipid and the activities of GOT and GPT was measured. The level of lipid and TBARS and the activity of GSH-Px was measured also. The results obtained were: 1) Food intake in the experimental group supplied hot water soluble extract from Angelicae Gigantis Radix was low significantly. 2) In the experimental group supplied hot water soluble extract from Angelicae Gigantis Radix of serum total lipid and total cholesterol was significantly low. 3) Serum GOT activity was significantly low in the experimental group supplied hotwater soluble extract from Angelicae Gigantis Radix. There was no difference of serum GPT activity between control and experimental group supplied hot water soluble extract from angelical Gigantis Radix. 4) There was no difference of lipid composition, TBARS level and GSH-px activity in liver between control and experimental group. The result of this study indicates that the hot water soluble extract from Angelicae Gigantis Radix was effective in decreasing the food intake of the experimental animals in the hypercholesterol diet and also effective in decreasing the levels of serum total lipid, total cholesterol and GOT activity. However it was not effective to change the level of lipid, TBARS and GSH-Px activity of liver.

• 한국지역사회생활과학회지
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• 제15권1호
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• pp.105-113
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• 2004

This study investigated the synergy effects of hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine on the lipid metabolism and antioxidant defense system of rats on hypercholesterol diets. Sprague-Dawley male rats were fed either a hypercholesterol diet with water or a hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine. The experimental groups consisted of the control group (CO), the group supplied with hot water soluble extract from Eleutherococcus senticosus(ES), and the group supplied with hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine(ESC). Eleutherococcus senticosus was extracted, made into 0.5% solution, and fed to the ES and ESC groups. A 3% carnitine diet was supplied to the ESC group. Hypercholesterol diets contained 18% beef tallow and 5% cholesterol. After 4 weeks of administering these diets, serum and liver were obtained and the level of serum lipid and the activities of GOT and GPT were measured. In addition, the level of liver lipid and TBARS and the activity of GSH-Px were measured. The results were as follows: 1) Weight gain and FER in the group supplied with hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine(ESC) was low significantly(P<0.05). 2) In the groups supplied with hot water soluble extract from Eleutherococcus senticosus(ES) and the group supplied with hot water soluble extract from Eleutherocorcus senticosus and dietarty carnitine(ESC), total serum cholesterol levels were both significantly low (P<0.05,P.0.01). 3) Serum GOT activity was significantly low(P<0.05) in the group supplied with hot water soluble extract from Eleutherococcus senticosus(ES) and in the group supplied with hot water soluble extract from Eleutherococcus senticosus and dietary carnitine(ESC). There was no difference between serum GPT activity in the control group and the groups supplied with hot water soluble extract from Eleutherococcus senticosus or dietary carnitine. 4) Liver triglyceride was low (P.0.05) in the group supplied with hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine(ESC). No difference was observed in other lipid composition, TBARS level, and GSH-px activity in liver between the control group and the experimental groups. In summary, the effects of hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine on the lipid metabolism and antioxidant defense system (such as weight gain, FER, total serum cholesterol and liver triglyceride) were low when Eleutherococcus senticosus and dietarty carnitine were supplied simultaneously.

• 대한예방한의학회지
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• 제22권2호
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• pp.77-107
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• 2018

Objectives : In present study, therefore, possible beneficial pharmacological activities of standard potato protein extracts (SPE) were observed on the mild diabetic obese mice. Methods : After end of 12 weeks of continuous oral administrations of three different dosages of SPE 400, 200 and 100 mg/kg, or metformin 250 mg/kg, analyzed the hepatoprotective, hypolipidemic, hypoglycemic, nephroprotective and anti-obesity effects, separately. In addition, liver antioxidant defense systems were additionally measured with lipid metabolism-related genes expressions and hepatic glucose-regulating enzyme activities for action mechanism. Results : All of diabetes and related complications including obesity were significantly inhibited by treatment of SPE 400, 200 and 100 mg/kg, dose-dependently, and they also dramatically normalized the hepatic lipid peroxidation and depletion of liver endogenous antioxidant defense system, the changes of the hepatic glucose-regulating enzyme activities, also changes of the lipid metabolism-related genes expressions including hepatic $AMPK{\alpha}1$ and $AMPK{\alpha}2$ mRNA expressions, dose-dependently. Especially, SPE 200 mg/kg constantly showed favorable inhibitory activities against type II diabetes and related complications as comparable to those of metformin 250 mg/kg in HFD mice, respectively. Conclusions : The present work demonstrated that SPE 400, 200 and 100 mg/kg showed favorable anti-diabetic and related complications including obesity refinement activities in HFD mice, through AMPK upregulation mediated hepatic glucose enzyme activity and lipid metabolism-related genes expression, antioxidant defense system and pancreatic lipid digestion enzyme modulatory activities.

• Preventive Nutrition and Food Science
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• 제11권4호
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• pp.289-297
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• 2006

This study examined the effect of hesperidin supplementation with an ethanol diet on lipid and antioxidant metabolism in rats. Male Sprague-Dawley rats were divided into two groups (n=10), and were assigned to one of two dietary categories: $E_8$, ethanol diet (50 g/L) for 8 wks; $E_8H_4$, ethanol diet for the first 4 wks and hesperidin (0.02%, w/w) supplemented ethanol diet for the last 4 wks. The plasma and hepatic lipids, hepatic cholesterol regulating enzyme activity, hepatic antioxidant enzyme activity and lipid peroxidation were determined. Supplementation with hesperidin for the last 4 wks during the 8 wks period of the ethanol diet, significantly increased the ADH activity. In conjunction with the chronic administration of ethanol, hesperidin supplementation resulted in a significant decrease in the hepatic cholesterol and triglyceride concentrations compared to the $E_8$ group. The hepatic HMG-CoA reductase and ACAT activities were significantly lower in the hesperidin-supplemented group. When comparing hepatic antioxidant enzyme activities, SOD, GSH-Px, and G6PD activities and GSH level were significantly higher in the $E_8H_4$ group than in the E8 group. Plasma TBARS levels were significantly lower in rats fed ethanol with hesperidin compared to the rats fed only ethanol; however, the hepatic TBARS levels were not significantly different between the groups. Accordingly, the additional hesperidin supplement with an ethanol diet might be effective for improving the hepatic lipid metabolism and antioxidant defense system.

• Nutrition Research and Practice
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• 제9권3호
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• pp.227-234
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• 2015

BACKGROUND/OBJECTIVES: We investigated the effects of a combination of grape pomace (Vitis labrusca, Campbell Early) and Omija fruit (Schizandra chinensis, Baillon) ethanol extracts on lipid metabolism and antioxidant defense system in diet-induced obese mice. MATERIALS/METHODS: Forty male C57BL/6J mice were divided into four groups and fed high-fat diet (control group, CON) or high-fat diet added 0.5% grape pomace extract (GPE), 0.05% Omija fruit extract (OFE) or 0.5% GPE plus 0.05% OFE (GPE+OFE) for 12 weeks. RESULTS: In contrast to the GPE- or OFE-supplemented groups, the GPE+OFE group showed significantly lower body weight and white adipose tissue weights than the CON group. Moreover, GPE+OFE supplementation significantly decreased plasma total cholesterol and increased the plasma HDL-cholesterol/total-cholesterol ratio (HTR) compared to the control diet. The hepatic triglyceride level was significantly lower in the GPE+OFE and GPE groups by increasing ${\beta}$-oxidation and decreasing lipogenic enzyme compared to the CON group. Furthermore, GPE+OFE supplementation significantly increased antioxidant enzyme activities with a simultaneous decrease in liver $H_2O_2$ content compared to the control diet. CONCLUSIONS: Together our results suggest that supplementation with the GPE+OFE mixture may be more effective in improving adiposity, lipid metabolism and oxidative stress in high-fat diet-fed mice than those with GPE and OFE alone.

• 한국작물학회지
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• 제58권4호
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• pp.443-450
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• 2013

Effects of newly developed functional rice grains Goami (high-amylose rice) and Nokwon (green-kerneled rice) on the glucose metabolism and antioxidative defense system in C57BL/6N mice under high fat diet condition were investigated. Animals were randomly divided into five groups (n = 8) and given experimental diets for eight weeks: normal control diet (NC), high fat diet (HF), and high fat diet supplemented with white rice (HF-WR), Goami rice (HF-GR), and Nokwon rice (HF-NR). At the end of the experimental period, the HF group exhibited markedly higher blood glucose level, insulin concentration, plasma lipid peroxidation and lower hepatic glycogen concentration than that exhibited by NC group. However, diet supplementation of Goami and Nokwon suppressed the high fat diet-induced hyperglycemia and oxidative stress through inhibition of the glucose-regulating enzymes and enhancement of the antioxidant enzymes activities. The results illustrate that the new functional rice Goami and Nokwon may be useful in the development of functional foods with preventive effect against high fat diet-induced hyperglycemia and oxidative stress.

• Asian-Australasian Journal of Animal Sciences
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• 제28권7호
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• pp.958-965
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• 2015

Lycopene, a red non-provitamin A carotenoid, mainly presenting in tomato and tomato byproducts, has the highest antioxidant activity among carotenoids because of its high number of conjugated double bonds. The objective of this study was to investigate the effect of lycopene supplementation in the diet on plasma lipid profile, lipid peroxidation and antioxidant defense system in feedlot lamb. Twenty-eight Bamei male lambs (90 days old) were divided into four groups and fed a basal diet (LP0, 40:60 roughage: concentrate) or the basal diet supplemented with 50, 100, and 200 mg/kg lycopene. After 120 days of feeding, all lambs were slaughtered and sampled. Dietary lycopene supplementation significantly reduced the levels of plasma total cholesterol (p<0.05, linearly), total triglycerides (TG, p<0.05) and low-density lipoprotein cholesterol (LDL-C, p<0.05), as well as atherogenic index (p<0.001), whereas no change was observed in high-density lipoprotein cholesterol (p>0.05). The levels of TG (p<0.001) and LDL-C (p<0.001) were decreased with the feeding time extension, and both showed a linear trend (p<0.01). Malondialdehyde level in plasma and liver decreased linearly with the increase of lycopene inclusion levels (p<0.01). Dietary lycopene intake linearly increased the plasma antioxidant vitamin E level (p<0.001), total antioxidant capacity (T-AOC, p<0.05), and activities of catalase (CAT, p<0.01), glutathione peroxidase (GSH-Px, p<0.05) and superoxide dismutase (SOD, p<0.05). The plasma T-AOC and activities of GSH-Px and SOD decreased with the extension of the feeding time. In liver, dietary lycopene inclusion showed similar antioxidant effects with respect to activities of CAT (p<0.05, linearly) and SOD (p<0.001, linearly). Therefore, it was concluded that lycopene supplementation improved the antioxidant status of the lamb and optimized the plasma lipid profile, the dosage of 200 mg lycopene/kg feed might be desirable for growing lambs to prevent environment stress and maintain normal physiological metabolism.

• 한국식품영양과학회지
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• 제36권12호
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• pp.1537-1543
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• 2007

본 연구에서는 포도씨열수추출물을 이용하여 고지방식이로 비만을 유도한 마우스의 지질대사 및 적혈구의 항산화방어계에 미치는 영향을 살펴보았다. 4주령의 C57BL/6 마우스(n=24)를 1주일간 적응시킨 후 정상식이를 급여한 정상군, 고지방(열량의 37%를 지방으로 대체)을 급여한 고지방대조군과 고지방-포도씨열수추출물군으로 나누어 6주간 사육하였다. 포도씨열수추출물은 1%수준으로 식이에 첨가하였으며 동일한 열량, 질소량 및 섬유소가 함유되도록 조제하여 급여하였다. 고지방식이는 정상식이에 비하여 체중, 일일 열량섭취량, 백색지방 무게, 혈장과 간조직의 지질함량 및 혈장 leptin 함량을 유의적으로 증가시켰다. 본 실험에서 첨가된 식이 1%의 포도씨열수추출물 보충은 고지방을 급여한 마우스의 체중과 장기무게에는 영향을 미치지 않았으나, 혈장의 중성지질과 간조직의 콜레스테롤 함량을 현저히 저하하는 것으로 나타났다. 특히, 혈장 중의 중성지질은 포도씨열수추출군에서 정상수준으로 개선되었으며, 포도씨열수추출물은 변으로 중성지질 배설을 고지방대조군에 비하여 유의적으로 높였다. 간조직의 지질대사 효소인 FAS, ${\beta}$-oxidation, CPT 활성은 고지방대조군에서 정상군에 비하여 유의적으로 낮았으나 포도씨열수추출물군의 CPT 활성은 고지방대조군에 비하여 유의적으로 높았고, ${\beta}$-oxidation 활성도 증가경향을 보였다. 또한 적혈구의 SOD와 GSH-Px 활성이 포도씨열수추출물 급여시 활성화될 뿐만 아니라 고지방식이로 유도한 비만마우스의 적혈구내 지질과산화물 함량이 유의적으로 낮아졌다. 이와 같이 포도씨열수추출물은 고지방 섭취시 혈 중 지질개선 및 항산화제로서의 가능성을 제시하였다.

• 한국식품영양과학회지
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• 제39권5호
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• pp.677-683
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• 2010

본 연구는 고지방을 급여한 마우스의 체내 지질개선 및 항산화 방어계에 미치는 갈대순의 영향을 구명하고자 하였다. 4주령의 ICR마우스(n=24)를 1주일간 적응시킨 후 정상식이를 급여한 정상군, 고지방을 급여한 고지방군, 고지방-갈대순분말 급여군으로 나누어 8주간 사육하였다. 갈대순분말은 사람이 하루에 3잔의 차를 마시는 양을 고려하여 식이에 1% 수준이 되도록 첨가 조제하여 급여하였다. 갈대순분말군의 체중, 일일 식이섭취량과 에너지섭취량은 고지방군에 비하여 감소하는 경향이었다. 갈대순분말 급여 시 혈장의 총 콜레스테롤 함량은 고지방군에 비하여 19% 감소되었고 중성지질 함량은 36% 유의적인 감소를 보였다. 갈대순분말은 고지방식이로 축적된 간 조직의 중성지질 함량 변화에는 영향을 미치지 않았으나 콜레스테롤 함량은 고지방군에 비하여 유의적으로 낮추었다. 심장조직의 중성지질과 콜레스테롤 함량 역시 정상군에 비하여 고지방군이 각각 145%, 112%의 유의적인 증가를 나타내었으나 갈대순분말 급여로 중성지질 함량은 감소경향을 보였으며 콜레스테롤 함량은 정상군과 유사한 수준으로 개선되었다. 갈대순분말은 고지방군에 비하여 분변으로의 중성지질과 콜레스테롤 배설을 각각 27%와 28% 유의적으로 증가시켰다. 갈대순분말은 간 조직의 지방산 산화에는 영향을 미치지 않았고 지방산 합성효소 활성을 유의적으로 억제하였다. 간 조직 중의 HMG-CoA reductase와 ACAT 활성도 역시 갈대순급여군이 고지방군에 비하여 유의적으로 낮았다. 적혈구 중의 SOD와 CAT 활성은 정상군에 비하여 고지방식이 급여 시 유의적인 증가를 보였으나 GSH-Px 활성은 고지방식이로 인한 변화가 관찰되지 않았다. 반면, 갈대순분말 급여는 적혈구의 SOD와 CAT 활성과 지질과산화물 함량을 고지방군에 비하여 유의적으로 낮추었다. 이와 같이 갈대순분말은 고지방식 이를 급여한 마우스의 간 조직에서의 지방산과 콜레스테롤 합성을 저해하고 분변으로의 지질배설을 증가시킴으로써 혈장 지질개선에 효과적이었으며 적혈구의 항산화 효소활성도와 지질과산화물 함량도 개선하는 것으로 나타났다.