• 한국식품영양과학회지
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• 제21권1호
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• pp.23-28
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• 1992

고형상의 모델시스템에서 리놀레산의 산화에 영향을 미치는 여러가지 인자들 즉 리폭시게나아제, 수분활성도, ${\beta}$-카로틴, ${\alpha}$-토코페롤등에 대해 관찰하여 다음과 같은 결과를 얻었다. 리폭시게나아제에 의한 리놀레산의 산화반응은 aw 0.72에서 aw 0.25에 보다 잘 일어났다. 그러나 이 시스템에 ${\beta}$-카로틴이나 ${\alpha}$-토코페롤을 반응시키게 되면 리놀레산의 산화를 크게 저해하였으며 ${\beta}$-카로틴보다는 ${\alpha}$-토코페롤이 지질과 산화 과정에서 항산화작용이 더 컸다. 즉, 유사한 조건에서 ${\beta}$-카로틴은 24시간의 유도기간을 보여 준 반면 ${\alpha}$-토코페롤은 72시간까지도 유도기간이 지속되었다. ${\beta}$-카로틴의 항산화작용은 수분활성도의 영향을 크게 받았으며 수분활성도가 높을수록 항산화 작용이 뛰어났고 또한 ${\beta}$-카로틴의 농도가 높을수록 CDA 생성이 적었다.

• 한국식품영양과학회지
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• 제24권1호
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• pp.67-73
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• 1995

Effects of tocopherols and $\beta$-carotene on the oxidation of the solid model system of a free fatty acid mixture (64.5% of linolic acid ; 26.4% of oleic acid ; 5.0% of palmitic acid) with tocopherols and $\beta$-carotene were studied. $\alpha$-tocopherol revealed an antioxidant activity at the concentration below 0.05%, however, it showed a prooxidant activity when the concentration was higher than 0.05%. The antioxidant activity of ${\gamma}$ -tocopherol was not affected by the concentrations in the range of 0.01~0.10% in the model and ${\gamma}$-tocopherol showed higher antioxidant activity than that of $\alpha$-tocopherol. It seemed that $\alpha$-tocopherol was unstable compared to ${\gamma}$-tocopherol during oxidation. $\beta$-carotene showed a weak antioxidative activity at the initial stage of this system while $\beta$-carotene showed a prooxidant activity in the presence of tocopherol. $\beta$ -carotene was highly susceptible to autoxidative degradation during oxidation.

• 한국식품영양과학회지
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• 제29권5호
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• pp.943-947
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• 2000

The purpose of this study was to investigate the extraction method of phenolic compounds from Rubus coreanum and antioxidative activity. antioxidative activities of Rubus coreanum were tested with ability of donating hydrogen to DPPH, and HPLC, fluorometry which measure the amount of MDA after reacting linoleic acid with $H_2O$$_2$, and LDL with $H_2O$$_2$ and FeCl$_2$. The most suitable extraction conditions of the phenolic compounds from Rubus coreanum was 3 times with 60% ethanol, and the yield of extract containing 35% moisture was 15.28%. In extraction efficacy of phenolic compounds, 60% ethanol was superior to water as extraction solvent, and extraction efficacy with 60% ethanol did not differ from disolving by water after evaporation of 60% ethanol extract. 60% ethanol extract of Rubus coreanum had an ability of hydrogen donating to DPPH, MDA determination showed the antioxidative effect with inhibition ratio of 77.91% on linoleic acid oxidation by addition of Rubus coreanum extract with the concentration of 1.500 ppm. and about 65.74% of LDL oxidation was inhibited by addition of 1,000 ppm.

• Preventive Nutrition and Food Science
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• 제9권2호
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• pp.167-173
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• 2004

The aim of this study was to investigate the antioxidant effect of medicinal plants used by diabetic patients. Fourteen medicinal plants were selected and antioxidant activity such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, inhibition effect of linoleic acid autoxidation and low density lipoprotein (LDL) oxidation, thiobarbituric acid (TBA) value were measured. The Cornus officinalis had DPPH radical scavenging activity of 84.79%, which was higher than the 78.79% for $\alpha$-tocopherol. Rosa rugosa Thunberg, Pueraria thundergiana Bentham, Artemisia princeps var. orientalis and Sasamorpha purpurascens Nakai also had high values. Extracts with higher DPPH radical scavenging activities had higher total phenol concentrations, and positive correlations between these parameters were found. Inhibitory activities of linoleic acid autoxidation, LDL oxidation and TBA value, used as indices of oxidative stress, were observed in most of the selected medicinal plants. The highest inhibitory activity for TBA value was observed in the extract of Pueraria thundergiana Bentham. Rosa rugosa Thunberg (75.50 %), Sasamorpha purpurascens Nakai (74.00 %), and Cornus officinalis (73.00 %) all had high antioxidant activity against linoleic acid autoxidation, similar to that of $\alpha$-tocopherol. The same 3 plants also had high in inhibitory activity against LDL oxidation. Therefore, we demonstrated that medicinal plants used as folk remedy by diabetic patients had antioxidant activity. Especially, Rosa rugosa Thunberg, Cornus officinalis, Pueraria thundergiana Bentham, Arlemisia princeps var. orientalis, and Sasamorpha purpurascens Nakai had high phenol concentrations which resulted in high values for DPPH radical scavenging. These same plants exhibited high values for inhibitory activities for TBA value, linoleic acid autoxidation and LDL oxidation, indices associated with lipid peroxidation.

• 한국식품영양과학회지
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• 제23권2호
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• pp.238-243
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• 1994

Changes of lipid components in modified oleoresin during cooking at high temperature were investigated. In preparation of the modified oleoresin, dried red pepper was milled to 100 mesh of size particle and extracted oily compounds by reduced pressure steam distillation . The rest part was reextracted and concentrated and concentrated. The extracts were combined. The same volume of water and 4% of polyglycerol condensed ricinoleate (PGDR) were added to the combined extract, and emulsified to make oleresin red pepper. Non-polar lipid components were quantified 3 times higher in the oleoresin than polar lipid components . The components of non-polar lipd was mainly triglyceride comprising 75.8%. The level of phosphatidyl choline and phosphatidyl ethanolamine were 38.6and 26.1%, respectively. linoleic acid was distinctively abundant (63.1%) and followed by palmitic acid, oleic acid, linolenic acid and stearic acid in the oleoresin. Oxidation of lipid at high temperature was principally affected by temperature rather than oxygen existence . With the result of oxidation , palmitic acid and myristic acid increased, however, oleic acid, linoleic acid, and linolenic acid decreased.

• 한국식품조리과학회지
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• 제11권5호
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• pp.536-541
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• 1995

The antioxidative effect of Aloe extracts on 0.1 M-linoleic acid was compared with some commercial antioxidants during storage at 50${\pm}$2$^{\circ}C$ for 20 hours, and on soybean oils at 60${\pm}$2$^{\circ}C$ for 30 days. In the oxidation of linoleic acid, antioxidative effects of various Aloe extracts and other antioxidants were shown as following orders: 3％ Aloe methanol extract > 1％ Aloe methanol extract > 0.02% BHT > 3％ Aloe ethyl acetate extract > 0.5％ Aloe methanol extract > 0.02%-Tocopherol>Aloe methanol extract, 0.1, 0.02％ > Aloe ethylacetate extract, 1, 0.5, 0.1, 0.02％ > control, while in the oxidation of soybean oil, l％ Aloe methanol extract > 0.02％ BHT > 3％ Aloe methanol extract > 0.02%-Tocopherol>control.

• Journal of Animal Science and Technology
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• 제48권1호
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• pp.107-114
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• 2006

CLA를 이용한 고품질 기능성 육계의 생산 가능성을 알아보기 위해 첨가수준과 급여기간에 따른 부위별 CLA 축적율과 지방산 조성 및 지방산패도를 분석하였다. CLA 축적율은 첨가수준이 증가할수록 유의적으로(P<0.05) 증가하였으며, 급여기간에 따라서도 다소 증가하는 경향을 보였다. 그러나 급여 1주와 급여 2주 및 3주의 비교에서 큰 차이는 나타나지 않았다. CLA 첨가수준에 따른 포화지방산은 증가하는 경향을 보였지만, 불포화 지방산은 감소하는 경향을 보였으며, 특히 CLA와 가장 비슷한 구조를 가지고 있는 linoleic acid(C18:2)는 CLA 함량이 증가한 만큼 감소하는 경향을 나타내었다. CLA 첨가수준에 따른 지방산패도의 변화는 첨가수준이 증가함에 따라 낮게 나타났으며, 대조구에 비해 처리구가 낮게 나타났다. 이상의 결론은 근육내 CLA 축적을 극대화 시키기 위한 최적의 급여기간은 1주이며, 지방산패도의 효과적인 억제를 위해서 첨가수준 4.8%가 가장 적합하다는 것을 나타낸다.

• 한국식품영양과학회지
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• 제23권6호
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• pp.954-958
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• 1994

The bleaching effect of chlorophyll a by two lipoxygenase isoenzymes (LOX-1, LOX-2) isolated from potato tuber(variety DEinma ) was studied. In the presence of LOX-1 or LOX0-2 with linoleic acid chlorophyll a bleaching occurred during two isoenzymes-mediated oxidation of linoleic acid. Chlorophyll a bleaching porceeded with decreasing in the formation of conjugated dienes form linoleic acidyb LOX-1 and LOX-2 . In the presence of chlorophyll a, LOX-2 showed a markable decrease inproduction of conjugated dienes from linoleic acid and a higher chlorophyll a bleaching activity. compared with LOX-1. These results suggest chlorophyll-bleaching reaction required intermediates formed during the peroxidation of linoleic acid by lipoxygenase isoenzymes, thus preventing formation of conjugated dienes.

• 생약학회지
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• 제35권4호통권139호
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• pp.338-345
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• 2004

Scavenging effects of DPPH radical and hydroxyl radical, inhibition of linoleic acid oxidation, inhibition of NO synthesis and iNOS expression were tested with extracts and chromatographic subfractions of Canavalia lineata obtained at Jeju island. Chloroform extract and its subfractions gave moderate effects on scavenging DPPH radical and hydroxyl radical, They also inhibited linoleic acid oxidation, and NO synthesis. Inhibition of NO synthesis resulted from the repression of iNOS gene expression. Ethyl acetate extract and its subfractions showed excellent effects on scavenging DPPH radical and hydroxyl radical, while they were cytotoxic.

• 한국식품저장유통학회지
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• 제9권1호
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• pp.67-73
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• 2002

수입 참깨가루로 제조한 참기름의 품질과 위생적 안정성에 대한 기초자료를 얻고자 통참깨와 참깨가루를 원료로한 참기름의 일반성분, 화학적 특성, 지방산, sesamol, sesamolin, sesamin 함량 및 AOM에 의한 유도기간 등을 비교 조사하였다. 일반성분 중 수분은 통참깨에 비해 참깨가루가 월등히 낮게 나타났으며, 단백질은 거의 차이가 없었다. 참기름의 화학적 특성으로 산가와 검화가는 참깨가루가 통참깨에 비해 높게 나타났으며, 요오드가는 통참깨군보다 낮았다. 참기름의 지방산 조성에서 linolenic acid과 linoleic acid의 함량은 각각 222.44~144.14, 2713~1776.46 $\mu\textrm{g}$/mL이었고, linoleic acid와 ${\gamma}$-linoleic acid의 함량이 통참깨 참기름에 비해 참깨가루 참기름의 함량이 낮았다. Lignan 물질 중 sesamol 함량은 통참깨 참기름이 참깨가루 참기름보다 함량이 높았고, 인도산 통참깨 참기름의 sesamol 함량이 가장 높았다. Sesamin, sesamolin 함량은 한국만 통참깨 참기름이 가장 높았다. AOM에 의한 산화유도시간은 북한산, 중국산 참깨가루가 각각 6.67, 13.35 시간으로 나타나 통참깨군보다 산화안정성이 낮은 것으로 나타났다.