• IMMUNE NETWORK
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• 제21권5호
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• pp.37.1-37.17
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• 2021

Hepatitis B virus X (HBx) protein has been reported as a key protein regulating the pathogenesis of HBV-induced hepatocellular carcinoma (HCC). Recent evidence has shown that HBx is implicated in the activation of autophagy in hepatic cells. Nevertheless, the precise molecular and cellular mechanism by which HBx induces autophagy is still controversial. Herein, we investigated the molecular and cellular mechanism by which HBx is involved in the TRAF6-BECN1-Bcl-2 signaling for the regulation of autophagy in response to TLR4 stimulation, therefore influencing the HCC progression. HBx interacts with BECN1 (Beclin 1) and inhibits the association of the BECN1-Bcl-2 complex, which is known to prevent the assembly of the pre-autophagosomal structure. Furthermore, HBx enhances the interaction between VPS34 and TRAF6-BECN1 complex, increases the ubiquitination of BECN1, and subsequently enhances autophagy induction in response to LPS stimulation. To verify the functional role of HBx in liver cancer progression, we utilized different HCC cell lines, HepG2, SK-Hep-1, and SNU-761. HBx-expressing HepG2 cells exhibited enhanced cell migration, invasion, and cell mobility in response to LPS stimulation compared to those of control HepG2 cells. These results were consistently observed in HBx-expressed SK-Hep-1 and HBx-expressed SNU-761 cells. Taken together, our findings suggest that HBx positively regulates the induction of autophagy through the inhibition of the BECN1-Bcl-2 complex and enhancement of the TRAF6-BECN1-VPS34 complex, leading to enhance liver cancer migration and invasion.

• 천문학논총
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• 제27권4호
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• pp.335-338
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• 2012

We present the $18{\mu}m$ luminosity function (LF) of galaxies at 0.006 < z < 0.8 (the average redshift is ~ 0.04) using the AKARI mid-infrared All-Sky Survey catalogue. We have selected 243 galaxies at $18{\mu}m$ from the Sloan Digital Sky Survey (SDSS) spectroscopic region. These galaxies then have been classified into five types; Seyfert 1 galaxies (Sy1, including quasars), Seyfert 2 galaxies (Sy2), low ionization narrow emission line galaxies (LINER), galaxies that are likely to contain both star formation and Active Galactic Nuclei (AGN) activities (composites), and star forming galaxies (SF) using optical emission lines such as the line width of $H{\alpha}$ or the emission line ratios of [OIII]/$H{\beta}$ and [NII]/$H{\alpha}$. As a result of constructing the LF of Sy1 and Sy2, we found the following results; (i) the number density ratio of Sy2 to Sy1 is $1.64{\pm}0.37$, larger than the results obtained from optical LF and (ii) the fraction of Sy2 in the entire AGN population may decrease with $18{\mu}m$ luminosity. These results suggest that most of the AGNs in the local universe are obscured by dust and the torus structure probably depends on the mid-infrared luminosity.

• 한국산업융합학회 논문집
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• 제26권2_2호
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• pp.341-347
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• 2023

The subway is one of the most common and important means of transportation in modern society. In order to use the subway safely, tracks are necessary, but trains are prone to derailment and collisions. In order for the train to run safely on the track, the fishplate that connects the line connection is used. The damaged railway was a fishplate for connecting subway lines used for 11 years, and damage analysis and countermeasures were presented. Beach marks were observed on both fracture surfaces, and striations appeared at the range of crack propagation. The damaged part is Cr carbide, which has a higher hardness than the base metal, and is judged to be embrittled and destroyed by fatigue. The SM50C fishplate was subjected to a cyclic stress of about 59% of the upper limit of tensile-compression fatigue limit, but inclusions were the cause of failure. In order to prevent fatigue failure of the SM50C steel fishplate, the occurrence of inclusions should be minimized and processed to have a homogeneous structure when manufacturing the fishplate. In addition, compressive residual stress is given through surface modification such as peening to control crack generation. It is necessary to minimize the change in shape that can become a stress concentration part along with accurate fastening of the bolt, and to design the stress distribution to be as uniform as possible.

• Bulletin of the Korean Chemical Society
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• 제34권10호
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• pp.2877-2882
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• 2013

Second-order rate constants ($k_{Ox^-}$) have been measured spectrophotometrically for nucleophilic substitution reactions of 4-nitrophenyl X-substituted-cinnamates (7a-7e) and Y-substituted-phenyl cinnamates (8a-8e) with butane-2,3-dione monoximate ($Ox^-$) in 80 mol % $H_2O$/20 mol % DMSO at $25.0{\pm}0.1^{\circ}C$. The Hammett plot for the reactions of 7a-7e consists of two intersecting straight lines while the Yukawa-Tsuno plot exhibits an excellent linearity with ${\rho}_X$=0.85 and r=0.58, indicating that the nonlinear Hammett plot is not due to a change in the rate-determining step but is caused by resonance stabilization of the ground state (GS) of the substrate possessing an electron-donating group (EDG). The Br${\o}$nsted-type plot for the reactions of Y-substituted-phenyl cinnamates (8a-8e) is linear with ${\beta}_{lg}$ = -0.64, which is typical of reactions reported previously to proceed through a concerted mechanism. The ${\alpha}$-nucleophile ($Ox^-$) is more reactive than the reference normal-nucleophile ($4-ClPhO^-$). The magnitude of the ${\alpha}$-effect (i.e., the $k_{Ox^-}/k_{4-ClPhO^-}$ ratio) is independent of the electronic nature of the substituent X in the nonleaving group but increases linearly as the substituent Y in the leaving group becomes a weaker electron-withdrawing group (EWG). It has been concluded that the difference in solvation energy between $Ox^-$ and $4-ClPhO^-$ (i.e., GS effect) is not solely responsible for the ${\alpha}$-effect but stabilization of transition state (TS) through a cyclic TS structure contributes also to the Y-dependent ${\alpha}$-effect trend (i.e., TS effect).

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2008년도 추계학술대회 논문집 Vol.21
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• pp.368-368
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• 2008

Ion implantation has been widely developed during the past decades to become a standard industrial tool. To comply with the growing needs in ion implantation, innovative technology for the control of ion beam parameters is required. Beam current, beam profile, ion fractions are of great interest when uniformity of the implant is an issue. Especially, it is important to measure the spatial distribution of beam power and also the energy distribution of accelerated ions. This energy distribution is influenced by the proportion of mass for ion in the plasma generator(ion source) and by charge exchange and dissociation within the accelerator structure and also by possible collective effects in the neutralizer which may affect the energy and divergence of ions. Hydrogen atom has been the object of a good study to investigate the energy distribution. Hydrogen ion sources typically produce multi-momentum beams consisting of atomic ion ($H^+$) and molecular ion ($H_2^+$ and $H_3^+$). In the beam injector, the molecular ions pass through a charge-exchanges gas cell and break up into atomic with one-half (from $H_2^+$) or one-third (from $H_3^+$) according to their accelerated energy. Burrell et al. have observed the Doppler shifted lines from incident $H^+$, $H_2^+$, and $H_3^+$ using a Doppler shift spectroscopy. Several authors have measured the proportion of mass for hydrogen ion and deuterium using an ion source equipped with a magnetic dipole filter. We developed an ion implanter with 50-KeV and 20-mA ion source and 100-keV accelerator tube, aiming at commercial uses. In order to measure the proportion of mass for ions, we designed a filter system which can be used to measure the ion fraction in any type of ion source. The hydrogen and helium ion species compositions are used a filter system with the two magnets configurations.

• Earthquakes and Structures
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• 제22권5호
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• pp.461-473
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• 2022

Energy-saving block and invisible multiribbed frame composite wall (EBIMFCW) is an important shear wall, which is composed of energy-saving blocks, steel bars and concrete. This paper conducted seismic performance tests on six 1/2-scale EBIMFCW specimens, analyzed their failure process under horizontal reciprocating load, and studied the effect of axial compression ratio on the wall's hysteresis curve and skeleton curve, ductility, energy dissipation capacity, stiffness degradation, bearing capacity degradation. A formula for calculating the peak bearing capacity of such walls was proposed. Results showed that the EBIMFCW had experienced a long time deformation from cracking to failure and exhibited signs of failure. The three seismic fortification lines of the energy-saving block, internal multiribbed frame, and outer multiribbed frame sequentially played important roles. With the increase in axial compression ratio, the peak bearing capacity and ductility of the wall increased, whereas the initial stiffness decreased. The change in axial compression ratio had a small effect on the energy dissipation capacity of the wall. In the early stage of loading, the influence of axial compression ratio on wall stiffness and strength degradation was unremarkable. In the later stage of loading, the stiffness and strength degradation of walls with high axial compression ratio were low. The displacement ductility coefficients of the wall under vertical pressure were more than 3.0 indicating that this wall type has good deformation ability. The limit values of elastic displacement angle under weak earthquake and elastic-plastic displacement angle under strong earthquake of the EBIMFCW were1/800 and 1/80, respectively.

• 생약학회지
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• 제37권4호
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• pp.278-282
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• 2006

In this study, we analyzed and quantified the contents of magnolol (1), honokiol (2), and obovatol (3) in the leaves of Magnolia species by HPLC. HPLC methods were developed and validated by ICH guide lines comparing the linearity, intraday precision, inter-day precision. The optimal conditions were as follows; wavelength: 288 nm, column: Zorbax Eclipse XDBC18 $(2.1\times150\;mm,\;5{\mu}m)$, mobile phase: Acetonitril - 0.1 % $H_3PO_4$ (45:55, v/v) buffer, flow rate: 0.5 ml/min. The contents of magnolol, honokiol and obovatol were 1.08%, 2.37%, and 0.57% in the leaves of M. officinalis, 0.50%, 0.03%, and 1.54% in the leaves of M. biloba, 0.37%, 0.03%, and 0.76% in the leaves of M. obovata. The contents of effective ingredients in the leaves of M. officinalis and M biloba were higher than that of M. obovata. Especially, the content of obovatol in the leaves of M. biloba was remarkably high. This result showed that the shapes of leaves have a certain relationship with the content of obovatol.

• Journal of Electrical Engineering and Technology
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• 제10권5호
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• pp.2070-2076
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• 2015

Nano composite materials were multi-constituent combinations of nano dimensional phases with distinct differences in structure, chemistry and properties. Nano particles were less likely to create large stress concentrations and thereby can avoid the compromise of the material ductility while improve other mechanical properties. Corona discharge was an electrical discharge. The ionization of a fluid surrounding a conductor was electrically energized. This discharge would occur when the strength of the electric field around the conductor was high enough to form a conductive region, but not high enough to cause electrical breakdown or arcing to nearby objects. This paper shows all the studies done on the preparation of nano fillers. Special attention has given to the ACSR transmission line conductor, TiO₂ nano fillers and also to the evaluation of corona resistance on dielectric materials discussed in detail. The measurement of the dielectric properties of the nano fillers and the parameters influencing them were also discussed in the paper. Corona discharge test reveals that in 0%N ACSR sample corona loss was directly proportional to the applied line voltage. No significant change in corona loss between 0%N and 1%N. When TiO₂ nano filler concentration was increased up to 10%N fine decrement in corona loss was found when compared to base ACSR conductor, corona loss was decreased by 40.67% in 10%N ACSR sample. It was also found from the surface conditions test that inorganic TiO₂ nano filler increases the key parameters like tensile strength and erosion depth.

• Computers and Concrete
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• 제31권5호
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• pp.405-417
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• 2023

The submerged floating tunnel (SFT) is tethered by mooring lines anchored to the seabed, therefore, the structural integrity of the anchor should be sensitively managed. Despite their importance, reaction forces cannot be simply measured by attaching sensors or load cells because of the structural and environmental characteristics of the submerged structure. Therefore, we propose an effective method for estimating the reaction forces at the seabed anchor of a submerged floating tunnel using a structural pattern model. First, a structural pattern model is established to use the correlation between tunnel motion and anchor reactions via a deep learning algorithm. Once the pattern model is established, it is directly used to estimate the reaction forces by inputting the tunnel motion data, which can be directly measured inside the tunnel. Because the sequential characteristics of responses in the time domain should be considered, the long short-term memory (LSTM) algorithm is mainly used to recognize structural behavioral patterns. Using hydrodynamics-based simulations, big data on the structural behavior of the SFT under various waves were generated, and the prepared datasets were used to validate the proposed method. The simulation-based validation results clearly show that the proposed method can precisely estimate time-series reactions using only acceleration data. In addition to real-time structural health monitoring, the proposed method can be useful for forensics when an unexpected accident or failure is related to the seabed anchors of the SFT.

• 한국전자파학회논문지
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• 제18권2호
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• pp.136-142
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• 2007

휴대폰 몸체 전류 변화를 이용하여 PIFA의 방사 패턴을 변화시키고, 휴대폰용 모노폴 안테나의 대역폭을 증가시키는 방법을 소개한다. 방사 패턴 변화를 위하여 후엽을 억제하는 변형된 접지면 구조를 제안하였으며, 그 결과 변형하기 전보다 0.5 dB의 이득 향상을 가져왔다. 대역폭 증가를 위하여 모노폴 안테나에 두 개의 공진 주파수를 형성시키도록 평행한 두 개의 기생 도체선을 추가하는 방법을 제안하였다. 대역폭은 341 MHz로 기존의 모노폴 안테나 구조보다 2배 이상 향상되었다.