• 한국전자파학회논문지
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• 제15권12호
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• pp.1178-1183
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• 2004

본 논문에서는 정사각형 패치 안테나에 핀 다이오드(PIN diode)를 사용하여 편파변환 특성을 갖는 새로운 형태의 안테나를 제안하였다. 제안된 안테나는 정사각형 패치면 위에 한 쌍의 스퍼라인(spur-line)과 스퍼라인 위에 핀 다이오드를 삽입한 구조이다. 스퍼라인은 핀 다이오드의 on/off특성에 의해 I형 슬롯라인(slot-line)과 L형 스퍼라인의 형태로 변환되어 편파 변환 특성을 나타내었다. 안테나의 측정 결과 선형편파로 동작할 때에는 -15 dB 이상의 낮은 교차 편파 특성을 나타내었고, 원형편파로 동작할 때에는 0.83 dB의 좋은 축비 특성을 나타내었다. 안테나의 -10 dB대역폭은 on/off상태에 따라 각각 $2.1\%$와 $4.1\%$의 특성을 나타내었다. 또한, 원형편파로 동작하는 안테나의 축비 대역폭은 $3.5\%$의 비교적 넓은 대역폭의 특성을 나타내었다. 따라서 제안된 안테나는 동일한 대역폭 안에서 편파 변환 특성을 나타낼 수 있기 때문에 단일 안테나를 이용하여 주파수를 재사용하는 시스템에서 사용할 수 있다.

• 한국미생물·생명공학회지
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• 제23권3호
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• pp.329-336
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• 1995

For the development of new antitumor antibiotics produced by microorganisms, Streptomyces sp. YBE-316 was isolated from soil. The productivity of the antitumor antibiotic from Streptomyces sp. YBE-316 gradually increased after 60 hours, and was maximum after 100 hours after inoculation in growth medium (2.0% sucrose, 1.0% soybean meal, 0.1% K$_{2}$HPO$_{4}$, pH 7.0) at 30$\circ$C, 150 rpm, 5 NL/min by 30 l jar fermentor. This antitumor antibiotic was present only in mycelium, and stable in pH 5.0-10.0 for 20 minutes at 100$\circ$C. Antitumor and antibiotic activities were maintained at neutral pH, and heat stability was low. This antitumor antibiotic was soluble in methanol and ethanol, and insoluble in water, ethyl acetate, chloroform, and n-hexane. This antitumor antibiotic was sequentially purified by acetone extraction from mycelium, butanol extraction, and silica gel column chromatography. Antitumor activity was low against most tested cell lines, but antibiotic activity was high and low against yeasts and bacteria, respectivelv. The visualization test showed that this antitumor antibiotic had higher hydroxyl, ketone, amino, carboxyl groups, and sugar(s) in its structure. Instrumental analyses showed that this antitumor antibiotic was a pentaene in polyene class antibiotics. In pentaene class antibiotics, this was considered as an eurocidin or capacidin type antibiotics. The molecular weight of this antitumor antibiotic was higher than 683.0 daltons, and this antitumor antibiotic might be glycosylated by other sugar(s), instead of mycosamine or perosamine, an amino sugar.

• 한국광학회지
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• 제13권4호
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• pp.289-294
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• 2002

2${\times}$2 MEMS 스위치와 광섬유 지연선로의 조합으로 구성된 위상배열 안테나(Phased-Array Antenna: PAA)용 광학적인 실시간 지연선로(True Time-Delay : TTD)를 제안하였으며, $30^{o}$의 분해능을 갖고 최대 $120^{o}$까지 빔 주사방향을 조정 할 수 있는 선형 PAA 용 실시간 지연선로를 구현하였다. 본 구조는 제어가 간편하며, 한 개의 파장 고정 레이저 다이오드를 사용하기 때문에 기존에 제안된 파장 가변 광원을 사용하는 구조들보다 고속 동작이 가능하고, 경제적이라는 장점을 갖고 있다. 또한, 제안된 실시간 지연선로는 각각 연결된 안테나 소자 4개로 구성된 10㎓용 PAA를 설계하였다. 전산 모의 실험 결과, 설계된 PAA의 최대 빔 이득은 빔 주사각이 0˚인 경우에는 11.6dB, $\pm$$30^{o}$일 때 11.2dB, 그리고 $\pm$$60^{o}$에서 10.6dB로 나타났다.

• 암석학회지
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• 제9권4호
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• pp.254-270
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• 2000

예천전단대가 통과하고 북동-남서 방향의 구조선과 동처 방향의 구조선이 발달하고 있는 경북 봉화군 상운면지역에서 북부 소백산육괴의 지질구조를 규명하기 위해 이 지역의 주 구성암류인 선캠브리아기 원남층과 중생대 각섬석 화강암에 대한 암석구조를 연구하였다. 이 지역의 지질구조는 원남층의 편마면 내지 편리면이 형성된 이후 적어도 네 번의 변형단계(예천전단대 형성과 관련된 D2 연성전단변형 이전에 한 번의 변형작용과 이후에 적어도 두 번의 변형작용)를 걸쳐 형성되었다. 북동-남서 방향의 구조선과 동서 방향의 구조선은 하나의 거대한 완사 내지 개방형 F4 습곡작용에 의해 형성되었으며, D4 변형 이전에 이들 구조선은 F4 습곡축부의 방향과 평행한 동북동서남서 방향이었던 것으로 해석된다. D4 변형 이전의 각 변형단계 별 구조적 특성 및 상대적인 발생시기는 다음과 같다: Dl 변형-준 수평적인 습곡축과 고각으로 경사하는 습곡축면을 갖는 동북동 방향의 등사습곡과 부딩구조 형성기; D2 변형-북쪽으로 경사하는 원남층의 우세한 엽리면의 상부가 동북동쪽으로 이동하는 대규모의 우수 주향 이동성의 연성전단운동에 의한 압쇄구조면과 신장선구조 그리고 Z자형 비대칭 습곡형성기(중생대 각섬석 화강암이 관입된 이후); D3 변형-원남층의 우세한 엽리면의 상부가 경사 방향(북북서 방향)으로 떨어지는 정전단운동에 의한 준 수령적인 습곡축과 습곡축면을 갖는 동북동 방향의 S자형 비대칭 습곡 형성기. 이러한 연구결과는 신동-봉화 연결선을 경계로 지구조선의 방위가 북동-남서 방향에서 동-서 방향으로 전환되는 북부 소백산육괴와 옥천대 북동부의 지구조 발달과정을 해석하는데 유용하게 활용될 것으로 기대된다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권2호
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• pp.615-618
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• 2016

Ethyl acetate extracts obtained from culture of endophytic fungi Aspergillus sp isolated from Piper crocatum Ruiz & Pav, have been shown to possess cytotoxic activity against T47D breast cancer cells. Investigations were here conducted to determine bioactive compounds responsible for the activity. Bioassay guided fractionation was employed to obtain active compounds. Structure elucidation was performed based on analysis of LC-MS, $^1H$-NMR, $^{13}C$-NMR, COSY, DEPT, HMQC, HMBC data. Cytotoxity assays were conducted in 96 well plates against T47D and Vero cell lines. Bioassay guided isolation and chemical investigation led to the isolation of pyrophen, a 4-methoxy-6-(1'-acetamido-2'-phenylethyl)-2H-pyran-2-one. Further analysis of its activity against T47D and Vero cells showed an ability to inhibit the growth of T47D cells with IC50 values of $9.2{\mu}g/mL$ but less cytotoxicity to Vero cells with an $IC_{50}$ of $109{\mu}g/mL$. This compound at a concentration of 400 ng/mL induced S-phase arrest in T47D cells.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.2009-2014
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• 2012

Objective: Taspine, isolated from Radix et Rhizoma Leonticis has demosntrated potential proctiective effects against cancer. Tas13D, a novel taspine derivative synthetized by structure-based drug design, have been shown to possess interesting biological and pharmacological activities. The current study was designed to evaluate its antiproliferative activity and underlying mechanisms. Methods: Antiproliferative activity of tas13D was evaluated by xenograft in athymic mice in vivo, and by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and cell migration assays with human liver cancer (SMMC-7721) cell lines in vitro. Docking between tas13D and VEGFR and EGFR was studied by with a Sybyl/Surflex module. VEGF and EGF and their receptor expression was determined by ELISA and real-time PCR methods, respectively. Results: Our present study showed that tas13D inhibited SMMC-7721 xenograft tumor growth, bound tightly with the active site of kinase domains of EGFR and VEGFR, and reduced SMMC-7721 cell proliferation (IC=34.7 ${\mu}mol/L$) and migration compared to negative controls. VEGF and EGF mRNAs were significantly reduced by tas13D treatment in a dose-dependent manner, along with VEGF and EGF production. Conclusion: The obtained results suggest that tas13D inhibits tumor growth and cell proliferation by inhibiting cell migration, downregulating mRNA expression of VEGF and EGF, and decreasing angiogenic factor production. Tas13D deserves further consideration as a chemotherapeutic agent.

• 한국결정성장학회:학술대회논문집
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• 한국결정성장학회 1996년도 The 9th KACG Technical Annual Meeting and the 3rd Korea-Japan EMGS (Electronic Materials Growth Symposium)
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• pp.258-292
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• 1996

In the last year great interest appears to YBCO thin films preparation on different substrate materials. Preparation of epitaxial film is a very difficult problem. There are many requirements to substrate materials that must be fullfilled. Main problems are lattice mismatch (misfit) and similarity of structure. From paper [1] or follows that difference in interatomic distances and angles of substrate and film is mire important problem than similarity of structure. In this work we present interatomic distances and angle relations between substrate materials belonging to ABCO4 group (where A-Sr or Ca, B-rare earth element, C-Al or Ga) of different orientations and YBCO thin films. There are many materials used as substrates for HTsC thin films. ABCO4 group of compounds is characterized by small dielectric constants (it is necessary for microwave applications of HTsC films), absence of twins and small misfit [2]. There most interesting compounds CaNdAlO4, SrLaAlO4 and SrLaGaO4 were investigated. All these compounds are of pseudo-perovskite structure with space group 14/mmm. This structure is very similar to structure of YBCO. SLG substrate has the lowest misfit (0.3%) and dielectric constant. For preparation of then films of substrates of this group of compound plane of <100> orientation are mainly used. Good quality films of <001> orientations are obtained [3]. In this case not only a-a misfit play role, but c-3b misfit is very important too. Sometimes, for preparation of thin films substrates of <001> and <110> orientations were manufactured [3]. Different misfits for different YBCO faces have been analyzed. It has been found that the mismatching factor for (100) face is very similar to that for (001) face so there is possibility of preparation of thin films on both orientations. SrLaAlO4(SLA) and SrLaGaO4(SLG) crystals of general formula ABCO4 have been grown by the Czochralski method. The quality of SLA and SLG crystals strongly depends on axial gradient of temperature and growth and rotation rates. High quality crystals were obtained at axial gradient of temperature near crystal-melt interface lower than 50℃/cm, growth rate 1-3 mm/h and the rotation rate changing from 10-20pm[4]. Strong anisotropy in morphology of SLA and SLG single crystals grown by the Czochralski method is clearly visible. On the basics of our considerations for ABCO4 type of the tetragonal crystals there can appear {001}, {101}, and {110} faces for ionic type model [5]. Morphology of these crystals depend on ionic-covalent character of bonding and crystal growth parameters. Point defects are observed in crystals and they are reflected in color changes (colorless, yellow, green). Point defects are detected in directions perpendicular to oxide planes and are connected with instability of oxygen position in lattice. To investigate facets formations crystals were doped with Cr3+, Er3+, Pr3+, Ba2+. Chromium greater size ion which is substituted for Al3+ clearly induces faceting. There appear easy {110} faces and SLA crystals crack even then the amount of Cr is below 0.3at.% SLG single crystals are not so sensitive to the content of chromium ions. It was also found that if {110} face appears at the beginning of growth process the crystal changes its color on the plane {110} but it happens only on the shoulder part. The projection of {110} face has a great amount of oxygen positions which can be easy defected. Pure and doped SLA and SLG crystals measured by EPR in the<110> direction show more intensive lines than in other directions which allows to suggest that the amount of oxygen defects on the {110} plane is higher. In order to find the origin of colors and their relation with the crystal stability, a set of SLA and SLG crystals were investigated using optical spectroscopy. The colored samples exhibit an absorption band stretching from the UV absorption edge of the crystal, from about 240 nm to about 550 m. In the case of colorless sample, the absorption spectrum consists of a relatively weak band in the UV region. The spectral position and intensities of absorption bands of SLA are typical for imperfection similar to color centers which may be created in most of oxide crystals by UV and X-radiation. It is pointed out that crystal growth process of polycomponent oxide crystals by Czochralski method depends on the preparation of melt and its stoichiometry, orientation of seed, gradient of temperature at crystal-melt interface, parameters of growth (rotation and pulling rate) and control of red-ox atmosphere during seeding and growth (rotation and pulling rate) and control of red-ox atmosphere during seeding and growth. Growth parameters have an influence on the morphology of crystal-melt interface, type and concentration of defects.

• Bulletin of the Korean Chemical Society
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• 제34권11호
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• pp.3257-3260
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• 2013

Two new diphenylethylenes, gramniphenols H and I (1 and 2), together with six known diphenylethylenes (3-8), were isolated from Arundina graminifolia. The structures of 1-8 were elucidated by spectroscopic methods including extensive 1D- and 2D-NMR techniques. Compounds 1 and 2 were evaluated for their cytotoxicity against five human tumor cell lines. Compound 1 showed cytotoxicity against PC3 cells with $IC_{50}$ value of 3.5 ${\mu}M$. Compound 2 showed cytotoxicity against NB4 and PC3 cells with $IC_{50}$ values of 3.6 and 3.8 ${\mu}M$, respectively.

• Journal of Ginseng Research
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• 제42권2호
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• pp.133-143
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• 2018

Background: AD-2 (20(R)-dammarane-3b, 12b, 20, 25-tetrol; 25-OH-PPD) is a ginsenoside and isolated from Panax ginseng, showing anticancer activity against extensive human cancer cell lines. In this study, effects and mechanisms of 1C ((20R)-3b-O-(L-alanyl)-dammarane-12b, 20, 25-triol), a modified version of AD-2, were evaluated for its development as a novel anticancer drug. Methods: MTT assay was performed to evaluate cell cytotoxic activity. Cell cycle and levels of reactive oxygen species (ROS) were determined using flow cytometry analysis. Western blotting was employed to analyze signaling pathways. Results: 1C concentration-dependently reduces prostate cancer cell viability without affecting normal human gastric epithelial cell line-1 viability. In LNCaP prostate cancer cells, 1C triggered apoptosis via Bcl-2 family-mediated mitochondria pathway, downregulated expression of mouse double minute 2, upregulated expression of p53 and stimulated ROS production. ROS scavenger, N-acetylcysteine, can attenuate 1C-induced apoptosis. 1C also inhibited the proliferation of LNCaP cells through inhibition on $Wnt/{\beta}-catenin$ signaling pathway. Conclusion: 1C shows obvious anticancer activity based on inducing cell apoptosis by Bcl-2 family-mediated mitochondria pathway and ROS production, inhibiting $Wnt/{\beta}-catenin$ signaling pathway. These findings demonstrate that 1C may provide leads as a potential agent for cancer therapy.

• Bulletin of the Korean Chemical Society
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• 제32권6호
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• pp.2015-2020
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• 2011

A series of isoxazol-indolin-2-one was designed for GSK-3${\beta}$ inhibitors as novel anticancer agents based on their binding mode analysis in GSK-3${\beta}$ crystal structure. Total 21 compounds were synthesized and evaluated for their inhibitory activity against two tumor cell lines (DU145 and HT29). Most of the synthesized compounds were potent with above 80% inhibitory activity at 100 ${\mu}M$, and several compounds were examined for inhibitory activity against GSK-3${\beta}$. Among them, 15(Z) ($R_1$=H, $R_2$=3-Cl-phenyl) was most active with 78% inhibition of tumor cell line (HT29) at 20 ${\mu}M$ and 72% inhibition of GSK-3${\beta}$ at 20 ${\mu}M$.