• Food Science and Biotechnology
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• 제18권1호
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• pp.113-117
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• 2009

A method was developed using enhanced liquid chromatography coupled with electrospray ionization mass spectrometry for the analysis and quantitation of 2 main potato glycoalkaloids, $\alpha$-chaconine, and $\alpha$-solanine, without any pre-concentration or derivatisation steps. Calibration curves generated by this technique exhibited a linear dynamic range from 0.025 to $50{\mu}g/mL$ and from 0.05 to $50{\mu}g/mL$ for $\alpha$-chaconine and $\alpha$-solanine, respectively. Matrix effects were evaluated by comparing calibration curves measured in matrix-matched and solvent-based systems. Ion suppression due to matrix effects was weak and extraction recoveries of 88 to 114% were obtained in different sample matrices spiked with analyte concentrations ranging from 15 to $35{\mu}g/mL$. Potatoes that had been genetically modified to tolerate glufosinate contained the same glycoalkaloid levels as their non-transgenic counterpart. We suggest complementing compositional comparison assessment strategy by validating quantitative analytical methods for the toxic glycoalkaloids in potato plants.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.595-602
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• 2005

A one-step real-time quantitative RT-PCR assay in combination with automated RNA extraction was evaluated for routine testing of HCV RNA in the laboratory. Specific primers and probes were developed to detect 302 bp on 5'-UTR of HCV RNA. The assay was able to quantitate a dynamic linear range of $10^7-10^1$ HCV RNA copies/reaction ($R^2=0.997$). The synthetic HCV RNA standard of $1.84{\pm}0.1\;(mean{\pm}SD)$ copies developed in this study corresponded to 1 international unit (IU) of WHO International Standard for HCV RNA (96/790 I). The detection limit of the assay was 3 RNA copies/reaction (81 IU/ml) in plasma samples. The assay was comparable to the Amplicor HCV Monitor (Monitor) assay with correlation coefficient r=0.985, but was more sensitive than the Monitor assay. The assay could be completed within 3 h from RNA extraction to detection and data analysis for up to 32 samples. It allowed rapid RNA extraction, detection, and quantitation of HCV RNA in plasma samples. The method provided sufficient sensitivity and reproducibility and proved to be fast and labor-saving, so that it was suitable for high throughput HCV RNA test.

• 전자공학회논문지
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• 제53권1호
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• pp.29-35
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• 2016

집적광학 $Ti:LiNbO_3\;1{\times}2$ Y-fed Balanced-Bridge 마하젠더 간섭기(YBB-MZI) 구조에 다이폴 패치 아테나를 적용해서 $1.3{\mu}m$ 파장대역에서 동작하는 전계센서를 구현하였다. BPM 전산모사를 통해서 소자 설계 및 동작성능을 검증하였고, $1.3{\mu}m$ 파장대역에서 ~16.6 V 스위칭전압과 이에 대응해서 소멸비는 ~14.7 dB로 측정되었다. 10 MHz, 50 MHz 각각의 주파수에서 감지 가능한 최소 전계는 1.12 V/m, 3.3 V/m로 측정 되었으며, 이에 대응되는 각 주파수에서 ~22 dB, ~18 dB의 다이나믹 범위가 측정되었다. 제작된 센서는 0.29~29.8 V/m 범위의 전계세기에 대해서 선형응답 특성을 나타내었다.

• Bulletin of the Korean Chemical Society
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• 제23권8호
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• pp.1139-1143
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• 2002

Analysis of lysophosphatidic acids (LPAs) is of clinical importance as they can serve a potential marker for ovarian and other gynecological cancers and obesity. It is critically important to develop a highly sensitive and specific method for the early detection of gynecological cancers to improve the overall outcome of this disease. We have established a novel quantification method of LPAs in human plasma by negative ionization tandem mass spectrometry (MS-MS) using multiple reaction monitoring (MRM) mode without the conventional TLC step. Protein-bound lipids, LPAs in plasma were extracted with methanol : chloroform (2:1) containing LPA C14:0 as an internal standard under acidic condition. Following back extraction with chloroform and water, the centrifuged lower phase was evaporated and reconstituted in methanol. The reconstituted solution was directly injected into electrospray source of MS/MS. For MRM mode, Q1 ions selected were m/z 409, 433, 435, 437 and 457 which corresponds to molecular mass [M-H]- of C16:0, C18:2, C18:1, C18:0 and C20:4 LPA, respectively. Q2 ions selected for MRM were m/z 79, phosphoryl product. Using MS/MS with MRM mode, all the species of LPAs were completely separated from plasma matrix without severe interferences. This method allowed simultaneous detection and quantification of different species of LPAs in a plasma over a linear dynamic range of 0.01-25 ㎛olL-1 . The detection limit of the method was 0.3 pmol/mL, with a correlation coefficient of 0.9983 in most LPAs analyzed. When applied to the plasmas of normal and gynecological cancer patients, this new method differentiated two different groups by way of total LPA level.

• Imaging Science in Dentistry
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• 제40권4호
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• pp.155-158
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• 2010

Digital radiographic systems allow the implementation of a fully digital picture archiving and communication system (PACS), and provide the greater dynamic range of digital detectors with possible reduction of X-ray exposure to the patient. This article reviewed the basic physical principles of digital radiographic imaging system in dental clinics generally. Digital radiography can be divided into computed radiography (CR) and direct radiography (DR). CR systems acquire digital images using phosphor storage plates (PSP) with a separate image readout process. On the other hand, DR systems convert X-rays into electrical charges by means of a direct readout process. DR systems can be further divided into direct and indirect conversion systems depending on the type of X-ray conversion. While a direct conversion requires a photoconductor that converts X-ray photons into electrical charges directly, in an indirect conversion, lightsensitive sensors such as CCD or a flat-panel detector convert visible light, proportional to the incident X-ray energy by a scintillator, into electrical charges. Indirect conversion sensors using CCD or CMOS without lens-coupling are used in intraoral radiography. CR system using PSP is mainly used in extraoral radiographic system and a linear array CCD or CR sensors, in panoramic system. Currently, the digital radiographic system is an important subject in the dental field. Most studies reported that no significant difference in diagnostic performance was found between the digital and conventional systems. To accept advances in technology and utilize benefits provided by the systems, the continuous feedback between doctors and manufacturers is essential.

• 한국지능시스템학회논문지
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• 제19권6호
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• pp.852-859
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• 2009

TFT-LCD 자동 검사 시스템에서 결함 검출을 위한 영상은 라인 스캔 카메라(line scan camera)나 에어리어 스캔 카메라 (area scan camera)에 의해서 획득하게 된다. 그러나 임펄스 잡음과 가우시안 잡음, CCD 혹은 CMOS 센서의 한계, 조명등의 영향으로 열화된 영상이 획득되며, 한도성 결함 영역을 인간의 육안으로 구분하기 어렵게 된다. 본 논문에서는 효율적인 결함 검출을 위해 특징 추출 방법과 결함 검출 방법을 제안한다. 특징 벡터로 웨버의 법칙을 이용한 결함 영역과 주변 배경 영역의 평균 밝기 차와 주변 배경 영역의 밝기 변화를 이용한 표준편차를 이용하며, 결함 영역 검출를 위해 추출된 특징 벡터를 이용하여 비선형 SVM을 적용한다. 실험 결과는 제안한 방법이 다른 방법들 보다 성능이 우수함을 보여준다.

• 천문학회보
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• 제39권1호
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• pp.54.1-54.1
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• 2014

Kyung Hee University has been developing a CubeSat for the space science mission called SIGMA (Scientific cubesat with Instrument for Global Magnetic field and rAdiation), which includes TEPC (Tissue Equivalent Proportional Counter) and a magnetometer. SIGMA has a 3-unit CubeSat, and the weight is about 3.2 kg. The main payload is TEPC which can measure the Linear Energy Transfer (LET) spectrum and calculate the equivalent dose for the complicated radiation field in the space. The magnetometer is a secondary payload using a miniaturized fluxgate magnetometer. We expect it to have a 1 nT resolution in the dynamic range of ${\pm}65535$ nT. An Attitude Control System (ACS) spins the SIGMA spacecraft 4 rpm with the spin axis perpendicular to the ecliptic plane. Full duplex communication is consists of VHF uplink and S-band and UHF downlink. In this paper, we introduce the system design and the scientific purpose of the SIGMA CubeSat mission.

• Nuclear Engineering and Technology
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• 제51권2호
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• pp.432-438
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• 2019

In the study of nuclear structure, the fast timing technique can be used to measure the lifetime of excited states. In the paper, we have developed a new fast timing system, which is made up of two $LaBr_3:Ce$ detectors and a set of waveform sampling system. The sampling system based on domino ring sampler version 4 chip (DRS4) can digitize and store the waveform information of detector signal, with a smaller volume and higher timing accuracy, and the waveform data are performed by means of digital waveform analysis methods. The coincidence time resolution of the fast timing system for two annihilation 511 keV ${\gamma}$ photon is 200ps (FWHM), the energy resolution is 3.5%@511 keV, and the energy linear response in the large dynamic range is perfect. Meanwhile, to verify the fast timing performance of the system, the $^{152}Gd-2_1^+$ state form ${\beta}^+$ decay of $^{152}Eu$ source is measured. The measured lifetime is $45.3({\pm}5.0)ps$, very close to the value of the National Nuclear Data Center (NNDC: $46.2({\pm}3.9)ps$). The experimental results indicate that the fast timing system is capable of measuring the lifetime of dozens of ps. Therefore, the system can be widely used in the research of the fast timing technology.

• Imaging Science in Dentistry
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• 제53권1호
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• pp.77-82
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• 2023

Purpose: This study evaluated anatomical variations in the root canals of the lower premolars and molars in a Brazilian sub-population using cone-beam computed tomography (CBCT). Materials and Methods: In total, 121 CBCT images of patients were selected from a database. All images contained lower first and second premolars and molars on both sides of the arch, fully developed roots, and no treatment, resorption, or calcifications. In each image, the root canals of the lower premolars and molars were evaluated according to the Vertucci classification in On-Demand 3D software in the multiplanar reconstruction with dynamic navigation. Twenty-five percent of the images were re-assessed to analyze intraobserver confidence with the kappa test. Data were statistically evaluated with linear regression to evaluate the correlations of anatomic variations with age and sex, and the Wilcoxon test to analyze the laterality of variations, with a significance level of 5%. Results: The intraobserver agreement (0.94) was excellent. In general, the root canals of lower premolars and molars showed a higher prevalence of type I than other Vertucci classification types, followed by type V in premolars and type II in molars. When the molar roots were evaluated separately, type II was more frequent in mesial roots and type I in distal roots. Although age showed no correlations with the results, sex and laterality showed correlations with tooth 45 and the lower second premolars, respectively. Conclusion: The lower premolars and molars of a Brazilian sub-population showed a wide range of root canal anatomic variations.

• 한국해안해양공학회지
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• 제7권2호
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• pp.156-162
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• 1995

스커트의 유연성을 고려한 2차원 오일붐 모델에 대한 수치해법을 개발하였다. 본 수치모델에서는 부체를 강체로, 스커트를 장력이 걸려 있는 막으로, 스커트의 아래끝엔 집중질량이 놓여 있다고 가정하였다. 유동은 포텐셜이라고 가정하였으며 부체와 스커트의 연결부에서는 변위가 연속이라는 운동학적 조건을 그리고 스커트의 아래끝에는 집중질량에 대한 동력학적인 조건을 부가하였다. 수치해법은 선형포텐셜유동 이론에 근거한 Green 함수방법에 기초를 두고 있다. 스커트의 변형을 미리 알 수 없으므로 방사 포텐셜(radiation potential)과 부체의 변위 그러고 스커트의 변형을 동시에 구하는 방식을 택하였다. Green 정리를 적용하여 얻은 적분방정식과 부체의 운동방정식 그리고 스커트의 변형 관계식을 이산화하여 방사포텐셜과 부체의 변위 그리고 스커트의 변위에 대한 선형대수 방정식을 얻었다. 수치계산결과에 의하면 스커트의 유연성이 부체의 운동응답을 다소 줄일 수 있으며 부체의 공진체계를 바꿀 수 있음을 확인하였다. 그리고 오일붐의 운동응답특성에 영향을 주는 인자들 중에서 스커트의 길이와 집중질량을 파라미터로 하여 오일붐 모델의 운동응답특성을 비교해 보았다. 스커트가 유연한 경우와 스커트가 강체인 경우의 저주파수 극한해는 거의 일치하고 있어 수치해의 타당성을 간접적으로 확인할 수 있었다.