• Korean Journal of Microbiology
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• v.45 no.3
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• pp.251-256
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• 2009

Leaf samples and bulbs showing characteristic symptoms of virus infection were collected from Gang-won, Chung-nam, and Jeju Province of Korea in 2008-2009. Three viruses, Lily symptomless virus (LSV), Lily mottle virus (LMoV), and Cucumber mosaic virus (CMV) were detected by RT-PCR. Virus-infected plant samples were identified; 12 plants with LSV, 20 plants with LMoV, and 1 plant with CMV. Of the twelve LSV infected samples, seven samples were found to be mix-infected with LMoV and LSV. Symptoms of LMoV and LSV mixed infection were fairly severe, like as vein clearing, leaf curling, leaf mottling, leaf mosaic, and yellow streaking. Mixed infection with LMoV and LSV was also found in lily bulbs which have been stored under unfavorable environmental conditions. LMoV predominated in our tests, whereas spread of Lilyvirus X (LVX) was not found. The nucleotide sequences of coat protein (CP) region of seven isolates (4 LMoV, 2 LSV, and 1 CMV) were compared with the corresponding regions of LMoV (AJ564636), LSV (AJ516059) and CMV(AJ296154). The nucleotide sequence homologies between reference viruses and seven isolates were 95-99%. Complete sequencing of seven isolates is necessary to obtain more information on the molecular characteristics of these viruses as well as to increase sensitivity and rapidity of viral detection.

• FLOWER RESEARCH JOURNAL
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• v.16 no.1
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• pp.36-43
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• 2008

This purpose of this study was to examine the effect of planting density, growing medium and strength of a nutrient solution (National Horticultural Research Institute's nutrient solution: HRI's) on the growth and development of Oriental hybrid lily 'Le Reve' in a box cultivation. The planting density with 14, 18 and 22 bulbs had sprouting one day earlier than other treatments. Planting density of 22 bulbs flowered first, while six bulbs flowered the last, indicating that higher planting densities led earlier flowering. The increasing planting density increased stem length of cut flowers. On the other hand, cut flower quality was improved when the planting density was lower. The incidence of physiological disorders such as blasting was more frequent in planting density of 22, 18, and 14, indicating that higher planting densities caused higher incidences of physiological disorders. All planting densities except 22 bulbs displayed superior results in width, weight, number, and scale weight of the bulbs. Greater planting densities led to inferior bulb enlargement and an increased decomposition rate. pH decreased in all treatments after the bulb enlargement and decreased more as the planting density increased. Contents of P, K, Ca, and Mg increased, while contents of K and Ca decreased, as the planting density increased. The rice hull+coir (1:1, v/v) treatment was better than others, but did not show that much of a difference. Moreover, in bulbs enlargement after cut flower harvest, lily medium and perlite+peat moss treatments showed superior results, and decomposition rate was the greatest in the rice hull+coir (1:1, v/v) treatment. In the HRI's solution strength treatment from the period of flower bud emergence to flower harvest, higher solution strengths gave better cut flower quality in terns of length, weight, and number of flowers. The non-treated control and one third strength of a HRI's solution hastened flowering, indicating that lower strengths led to earlier flowering. According to the results of leaf analysis as affected by solution strength during the flower harvest, absorption rates of N and K were greater when the strength was higher, and Ca and Mg showed the same tendency. On the other hand, the absorption rate of P was the lowest in all treatments.

• Korean Journal of Agricultural Science
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• v.40 no.4
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• pp.281-287
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• 2013

In Korea, isolated cultivation has been implemented for 102 genera, including about 250 species, each of which has underwent microscopic inspection, cultivation of bacteria in selective medium, analysis of physiology and biochemistry, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). The number of isolated microorganisms was 8,307 in the period of 2005-2012, and bulbs and tubers had the greatest diversity of microorganisms, of 5,165 (62.2%), followed by 2,119 (25.0%) sapling, 796 (9.6%) seed, 150 (1.8%) cutting slip, 70 (0.8%) branch graft and 7 (0.1%). The number of cases which were disqualified were 413 (4.97%), after the detection of 47 disease causing species of microorganism. Viruses predominated, with 27 species, followed by 16 fungi, a viroid, a Chromalveolata and 2 further species. Top on the list of detection was Arabis mosaic virus (77 cases), followed by Tobacco rattle virus (70 cases), Lily symptomless virus (46 cases) and Penicillium expansum (46 cases).

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.207-210
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• 2010

An Asiatic lily (Lilium spp.) cultivar 'Onnuri' for cut and pot flower was developed in 2006 at Jeollabukdo Agricultural Research & Extension Services, Iksan, Korea. The cross was made in 1999 between 'Avignon', orange colored, and 'Nove Cento', yellow colored. A line was preliminarily selected as 'A99-6-48' by reason of stable flower color and shape among germinated seedlings. It had been multiplicated through in vitro culture and then bulbed on field during 2002~2003. After characteristics were investigated from 2004 to 2005, finally it was named as an 'Onnuri' in 2006. Flower of 'Onnuri' belongs to orange-red group with its center shown orange group. Plant height is 92.8 cm, flower size is medium as 13.8 cm. Stigma color is red-brown and blooming direction is upward-facing. Flowering time is mid-June.

• Journal of Plant Biotechnology
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• v.30 no.3
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• pp.251-255
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• 2003

An efficient system for in vitro bulblet formation of Lilium oriental hybrids(cvs). Casa Blanca and Siberia is described. Transverse thin cell layer(tTCL)(1mm thick) explants of 'Casa Blanca' formed bulblets at a frequency of 97.7％ when cultured on Murashige and Skoog (MS) medium supplemented with 1mg/L 2,4 dichlorophenoxyacetic acid(2,4-D) (On average 15.6 bulblets were formed per explant). The frequency of bulblet formation was drastically reduced when the explant ghickness was thinner than 1 mm. Explants from the outermost layer of bulb scale produced greater frequency of bulblet formation than middle or innermost layer. Among auxins supplemented to culture medium at 1 mg/L, 2,4-D led to greater frequency of bulblet formation on explants than dicamba, picamba, or phenylacetic acid(PAA). tTCL explants from the middle region of the outermost layer bulb scale yielded greater frequency of bulblet formation than the upper or lower region. tTCL stem explants of 'Siberia' formed bulblets at a frequency of 95.3% when cultured on MS medium with 1 mg/L 2,4-D(On average 9.1 bulblets were formed per explant). The system estabilished in this study will be useful for in vitro rapid propagation and genetic transformation of Lilium Oriental hybrids.

• FLOWER RESEARCH JOURNAL
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• v.19 no.1
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• pp.1-7
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• 2011

This study was carried out to establish a production system of cut flowers and bulbs of oriental hybrid lilies in highland in summer. In study, effect of cultivation type and shading was examined. The cultivation types used in the bulb production experiments were greenhouses with no cover, greenhouses with net screen (net screen house), and greenhouses with EVA roof cover and net screen sides (rain shelter house). Net screen and rain shelter houses had either 0 or 30% shading. Cultivation type had significant effect and shading had a minor effect on bulb production. The greatest yield and quality of produced bulbs were obtained in the rain shelter house with 30% shading. From the results of this study, it is concluded that dispersed production of high quality cut lily flowers and bulbs could be achieved during the summer in highland.

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.220-223
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• 2010

A FA intersectional hybrid lily cultivar 'Cherry Pink' was developed in 2006 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) Korea. The cross and immature embryo rescue was conducted between female parents Lilium FA hybrid 'Supia' and male parents L. Asiatic hybrid 'A94-9' by cut style pollination method (CSM) at Suwon in 1999. The first selection was done and was tentatively named as 'FA01-17' in 2001. After in vitro multiplication, bulbing production of 'FA01-17' line, growth and flowering characteristic tests were conducted from 2002 to 2005. The evaluation of characteristics and consumer preferences were surveyed at a lily flower show of NIHHS at Taean in 2006. 'Cherry Pink' flowered in the middle of June and grew more than 120 cm stem in length. Flowers bloomed facing upward, a little spotted in petals and deep pink (RHS, RP27D). The pollen of 'Cherry Pink' was sterile. Year-round flowering can be done by storing the bulb under $-1.5^{\circ}C$ conditions. It was needed to control Botrytis disease in wet season.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.654-658
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• 2010

A FA (Lilium formolongi $\times$ Lilium asiatic hybrid) intersectional hybrid lily cultivar 'Orange Crown' was developed in 2007 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) of Korea. The cross and immature embryo rescue was conducted between female parents 'Migreen' (Lilium formolongi 'Raizan' $\times$ Lilium asiatic hybrid 'A61') and male parent 'A01-187' (L. asiatic 'A96-28' $\times$ 'Sanzio') by cut style pollination method at Suwon in 2001. It was preliminarily selected as 'FA04-27' in 2004. Multiplication, bulb production and characteristic tests were conducted from 2004 to 2007. The evaluation of characteristics and preference were surveyed at a lily flower show of NIHHS at Taean in 2007. 'Orange Crown' flowers in the late of June and grows more than 183 cm stem length. Flowers bloom upward-facing, a little spotted and orange (RHS, O24A) petals. The pollen of 'Orange Crown' was sterile.

• Korean Journal of Plant Tissue Culture
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• v.28 no.6
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• pp.341-346
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• 2001

To investigate the effects of MS medium strength and nitrogen concentration on bulblet formation from bulblet scale segment culture and bulblet growth from bulblet of Lilium oriental hybrid 'Casa Blanca', asiatic hybrid 'Mona', and longiflorum hybrid 'Hinomoto', 0.5∼2.0 strength of MS salts and 30∼120 mM nitrogen concentrations of MS medium were examined in vitro. The number of bulblets from bulb scale segment was favored in the strength of 0.5∼1.0 strength of MS salts or 30 mM total nitrogen concentration of MS medium in three cultivars. But the growth of bulblets formed in vitro was promoted in the 2 strength of MS medium or hight concentration of total nitrogen of MS medium up to 120 mM in three experimented cultivars.

• FLOWER RESEARCH JOURNAL
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• v.16 no.3
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• pp.211-217
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• 2008

To build up data for mutation breeding of lily, sensitivity to irradiation of bulb, seed and pollen was investigated. The bulbs of seven cultivars including 'New Wave' were very sensitive to gamma rays. Non-irradiated bulbs produced plants with bloom as normal. However, irradiated bulbs showed retarded growth and did not flower. Bulbs of 'New Wave' and 'Tiny Dino' irradiated with 125Gy gamma rays survived, but their growth rate dropped considerably. Part or whole leaves of the other cultivars except 'New Wave' and 'Tiny Dino' withered. Moisture content of individual scale was 72~78% depending on cultivar. Bulblet formation from untreated scales was 100%. Among all irradiated scales only 'Siberia' scales showed 8.5% bulblet formation when irradiated with 50Gy gamma rays. Pollen germination as affected by gamma ray irradiation had no consistent tendency. When dosage of $F_1$ 'Augusta' seeds reached 300Gy, their survival ratio was drastically decreased. Bulblet formation in vitro was observed only in non-irradiated seeds. Lethal dose ($LD_{50}$) of seed was less than 100Gy. Height and width of $F_1$ 'Augusta' bulblets in vitro irradiated with gamma rays decreased as dosage increased regardless of the culture method. The percentage shoot formation was 100% in non-irradiated bulblets and 15~60% in irradiated ones.