• Title/Summary/Keyword: ligninolytic enzyme systems

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Screening of Outstanding White Rot Fungi for Biodegradation of Organosolv Lignin by Decolorization of Remazol Brilliant Blue R and Ligninolytic Enzymes Systems (Remazol Brilliant Blue R 탈색능과 리그닌 분해 효소시스템을 이용한 유기용매 리그닌 생분해 우수 균주 선별)

  • Hong, Chang-Young;Kim, Ho-Yong;Jang, Soo-Kyeong;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.41 no.1
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    • pp.19-32
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    • 2013
  • In this study, outstanding white rot fungi for biodegradation of organosolv lignin were selected on the basis of their ligninolytic enzyme system. Fifteen white rot fungi were evaluated for their ability to decolorize Remazol Brilliant Blue R (RBBR) in SSC and MEB medium, respectively. Six white rot fungi (Ceriporiopsis subvermispora, Ceriporia lacerate, Fomitopsis insularis, Phanerochaete chrysosporium, Polyporus brumalis, and Stereum hirsutum) decolorized RBBR rapidly in SSC medium within 3 days. The protein contents as well as the activities of manganese peroxidase (MnP) and laccase for 6 selected fungi were determined on the SSC medium with and without organosolv lignin. Interestingly, extracellular protein concentrations were determined to relative higher for S. hirsutum and P. chrysosporium in the presence of organosolv lignin than others. On the other hands, each fungus showed a different ligninolytic enzyme pattern. Among them, F. insularis resulted the highest ligninolytic enzyme activities on incubation day 6, indicating of 1,545 U/mg of MnP activity and 1,259 U/mg of laccase activity. In conclusion, $STH^*$ and FOI were considered as outstanding fungi for biodegradation of organosolv lignin, because $STH^*$ showed high extracellular protein contents and ligninolytic enzyme activities over all, and ligninolytic enzyme activities of FOI were the highest among white rot fungi used in this study.

Decolorization of dye solution using membrane bioreactor (MBR) by Trametes versicolor (막생물반응기(MBR)에 의한 염료용액의 처리연구)

  • Lee, Yuri;Kim, Hyun-Gi;Park, Chulhwan;Lee, Byunghwan;Kim, Sangyong
    • Clean Technology
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    • v.10 no.3
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    • pp.131-137
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    • 2004
  • Due to the low biodegradability of dyes, conventional biological wastewater treatment systems are inefficient in treating textile wastewater. In this study, white rot fungus, Trametes versicolor KCTC 16781, were investigated for the decolorization of Reactive black 5 solutions. This fungus was able to degrade the dye solutions by the ligninolytic enzymes (laccase and MnP) produced. The enzyme activity remained constant until the end of reaction. The combined process of biological treatment and ceramic membrane showed better efficiency for decolorization and TOC removal than each single process.

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