• Journal of the Korean Applied Science and Technology
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• v.12 no.1
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• pp.109-114
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• 1995

The Synthesis of long chain fatty acid containing azobenzene and $(C_{n}-Azo)$ was optimized, starting from p-(p'-hydroxy phenyl azo)-benzoic acid and the product of reaction containing azobenzene chromophores was investigated by ultraviolet spectrophotometery in chloroform solvent at the various temperature. In addition, Reversibility and stability of azo compounds have been measured by means of Ultraviolet and the structure of these compound were ascertained by means of FT-IR and NMR. Recrystallization of reaction product in the solvent results the experimental yield obtained about 62.93% p-(p'-octadecyloxy phenyl azo)-benzoic acid. Long chain azobenzene derivatives in chloroform solution are induced photoisomerization by u. v. and visible light irradiation. The solution of long chain fatty acids$(C_{n}-Azo)$ containing azobenzene are possible of being applied to functional molecular devices such as photomemory and light switching.

• Elastomers and Composites
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• v.54 no.1
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• pp.14-21
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• 2019

Main-chain/side-chain liquid crystalline polymers (MCSCLCPs) combined with an azobenzene group and a cholesteryl group were synthesized to impart light and temperature sensitivity to the polymer. The polymers were designed with the azobenzene unit as the mesogenic group of the main-chain and various compositions of the azobenzene and cholesteryl units as the mesogenic group of the side-chain. The chemical structures and physical properties of the synthesized polymers were investigated by Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, differential scanning calorimetry, thermogravimetric analysis, polarized optical microscopy, and ultraviolet-visible (UV-Vis) spectroscopy. All the MCSCLCPs were amorphous and exhibited enantiotropic liquid crystal phases; these polymers achieved the nematic phase with increasing content of the azobenzene group and exhibited the cholesteric phase with weak liquid crystallinity as the content of the cholesteryl group was increased. Furthermore, the polymers containing the azobenzene group showed photoisomerization when exposed to UV-Vis light, and the CP-A3C7 and CP-A5C5 polymers exhibited thermochromism in the temperature range of the liquid crystal phase.

• Journal of the Korean Society for Precision Engineering
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• v.34 no.3
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• pp.211-215
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• 2017

This research analyzes bucket elevator roller chain pins by finite element (FE) analysis and static structural analysis for a lightweight pin design. The stress distribution of light weight roller chain pins under static load is analyzed for safety factors and damping effect. The results show that the stress distribution is higher on the plate than on the bush pin. In order to compare experimental and FE analysis results, a light weight design approach was used to produce a prototype base pin. Because the inner diameter of the pin was different, the impact damping effect was most appropriate when the inner diameter was 34.05 mm, and it is used as basic research data on the impact of the roller chain and sprocket.

• The Korean Journal of Physiology and Pharmacology
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• v.26 no.6
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• pp.479-499
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• 2022

The lack of a clonal renin-secreting cell line has greatly hindered the investigation of the regulatory mechanisms of renin secretion at the cellular, biochemical, and molecular levels. In the present study, we investigated whether it was possible to induce phenotypic switching of the renin-expressing clonal cell line As4.1 from constitutive inactive renin secretion to regulated active renin secretion. When grown to postconfluence for at least two days in media containing fetal bovine serum or insulin-like growth factor-1, the formation of cell-cell contacts via N-cadherin triggered downstream cellular signaling cascades and activated smooth muscle-specific genes, culminating in phenotypic switching to a regulated active renin secretion phenotype, including responding to the key stimuli of active renin secretion. With the use of phenotype-switched As4.1 cells, we provide the first evidence that active renin secretion via exocytosis is regulated by phosphorylation/dephosphorylation of the 20 kDa myosin light chain. The molecular mechanism of phenotypic switching in As4.1 cells described here could serve as a working model for full phenotypic modulation of other secretory cell lines with incomplete phenotypes.

• Journal of Life Science
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• v.26 no.8
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• pp.963-969
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• 2016

Kinesin superfamily proteins (KIFs) are microtubule-dependent molecular motor proteins essential for the intracellular transport of organelles and protein complexes in cells. Kinesin 1 is a member of those KIFs that transport various cargoes, including organelles, synaptic vesicles, neurotransmitter receptors, cell signaling molecules, and mRNAs through interaction between its light chain subunit and the cargoes. Kinesin light chains (KLCs) are non-motor subunits that associate with the kinesin heavy chain (KHC) dimer. KLCs interact with many different binding proteins, but their particular binding proteins have not yet been fully identified. We used the yeast two-hybrid assay to identify proteins that interact with the tetratricopeptide repeat (TPR) domain of KLC1. We found an interaction between the TPR domain of KLC1 and Wiskott-Aldrich syndrome protein family member 1 (WAVE1), a member of the WASP/WAVE family involved in regulation of actin cytoskeleton. WAVE1 bound to the six TPR domain-containing regions of KLC1 and did not interact with KHCs (KIF5A, KIF5B, and KIF5C) in the yeast two-hybrid assay. The carboxyl (C)-terminal verprolin-cofilin-acidic (VCA) domain of WAVE1 is essential for interaction with KLC1. Also, other WAVE isoforms (WAVE2 and WAVE3) interacted with KLC1 in the yeast two-hybrid assay. When co-expressed in HEK-293T cells, WAVE1 co-localized with KLC1 and co-immunoprecipitated with KLC1 and KIF5B. These results suggest that kinesin 1 motor protein may transport WAVE complexes or WAVE-coated cargoes in cells.

• Journal of Sericultural and Entomological Science
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• v.53 no.2
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• pp.124-129
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• 2015

We have previously characterized the complete fibroin light chain gene from one of the silkworm race Baegokjam (Bombyx mori) and found two variable regions (FibL1, intron 2 ~ 3; FibL2, intron 6) with the primer sets designed to cover these variable regions. In this study, we tested the utility of these variable regions as genetic markers for classifying silkworm races. For the purpose, Europian races (Q, PK), Chinese races (C26, C31), Japanese races (N15, N9), and tropical races (SA2, SA5) were used in this experiment. The polymorphism of the FibL1 and FibL2 were divided into two and three types, respectively. The combination of the FibL1 and FibL2 polymorphisms were constant within the same races. The result suggest that the primer sets designed from two variable regions of fibroin light chain gene may be useful as the genetic markers for silkworm races.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37 no.3C
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• pp.224-232
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• 2012

Priority MAC is an important issue in every communication system when we consider differentiated service applications. In this paper, we propose a mechanism to support priority MAC based on multi-parameters for IEEE 802.15.7 visible light communication (VLC). By using three parameters such as number of backoff times (NB), backoff exponent (BE) and contention window (CW), we provide priority for multi-level differentiated service applications. We consider beacon-enabled VLC personal area network (VPAN) mode with slotted version for random access algorithm in this paper. Based on a discrete-time Markov chain, we analyze the performance of proposed mechanism under non-saturation environments. By building a Markov chain model for multi-parameters, this paper presents the throughput and transmission delay time for VLC system. Numerical results show that we can apply three parameters to control the priority for VLC MAC protocol.

• BMB Reports
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• v.41 no.5
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• pp.394-399
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• 2008

Multiple sequence alignments of the Bombyx mori fibroin light chain gene (fib-L) from hybrids and from Chinese and Japanese strains demonstrated that 51.6% of the fib-L third intron is conserved. One of these conserved segments, 41 bp long, contains the sequence CGTTATTATACATATT, which is duplicated in the B. mori Nd-$s^D$ mutant. In the present work, electrophoretic mobility assays and computational analyses revealed a major peak of intrinsic bent DNA within the segment that undergoes breakage in the previously-described Nd-$s^D$ mutation. This result suggested that this intrinsically-curved region might mediate DNA cleavage and enhance recombination events in the third intron of the Bombyx mori fib-L gene.

• Bulletin of the Korean Chemical Society
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• v.20 no.9
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• pp.1010-1016
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• 1999

We synthesized the photoresponsive side chain polymers containing aminonitro azobenzene for studying the effect of temperature on photoinduced birefringence. Four different copolymers were prepared using methacrylate, α-methylstyrene, and itaconate monomer. Photoisomerization was observed under the exposure of UV light using UV-VIS absorption spectroscopy. Reorientation of polar azobenzene molecules induced optical anisotropy under a linearly polarized light at 532 nm. The change of the birefringence was observed with increasing the sample temperature under a continuous irradiation of excitation light. We could estimate the activation energy of molecular motion in thermal and photochemical mode. Besides the effect of glass transition temperature on the activation energy, we focused our interests on the effect of geometrical hindrance of polar azobenzene molecules and cooperative motion of environmental mesogenic molecules in the vicinity of polar azobenzene molecules.

• Proceedings of the KIEE Conference
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• 1997.07d
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• pp.1585-1587
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• 1997

We investigated the generation of high pretilt angle for nematic liquid crystal (NLC) in the cell with slanted non-polarized ultraviolet (UV) light irradiation on two kinds of the polyimide (PI) film. It was shown that the monodomain alignment in NLC is obtained in the cell with slanted non-polarized UV light irradiation on PI surface. The pretilt angle of NLC is generated about 3 degrees in the cell with slanted non-polarized UV light irradiation with 70 degrees on PI surface without side chain. But, the pretilt angle of NLC is generated about 1 degree in the cell with slanted non-polarized UV light irradiation with 80 degrees on PI surface with side chain. We consider that the pretilt angle generation in NLC is attributted to anisotropic dispersion force between the LC molecular and the PI surface.