• 한국임상수의학회지
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• 제34권2호
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• pp.123-125
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• 2017

An eight-year-old Shih-Tzu dog was referred to Kyungpook National University Veterinary Medical Teaching Hospital because of severe glaucoma and exophthalmos. The symptoms included severe buphthalmos, conjunctival hyperemia, focal dried cornea and discomfort in the affected eye. Although enucleation was considered as a potential intervention measure in light of the severity of symptoms, it was possible to save the globe and relieve symptoms through evisceration, intraocular prosthesis implantation, and lateral canthoplasty. Our experience with this case suggests that symptomatic salvage therapy, rather than enucleation, is an appropriate approach for resolution of exopthalmos and other complications associated with glaucoma surgery.

• Journal of Chest Surgery
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• 제52권3호
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• pp.165-169
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• 2019

Phlegmonous esophagitis must be treated aggressively; therefore, appropriate antibiotic therapy and drainage are critical. Although a conventional surgical approach has been used previously, internal drainage could be another treatment option in light of advances in endoscopic techniques. We report 2 cases in which patients suffering from phlegmonous esophagitis were successfully treated with endoscopic intraluminal drainage and antibiotics.

• Archives of Plastic Surgery
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• 제48권1호
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• pp.127-130
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• 2021

Rectovaginal fistula, which can arise after an injury to the vaginal canal or rectum, is a troublesome obstacle for patients' everyday life. In most cases, it can be covered with a local flap, but previous radiation therapy increases the recurrence rate, making it especially difficult to cure. As the application of stromal vascular fraction (SVF) obtained from enzymatically digested autologous adipose tissue has become increasingly common, several reports have advocated its effectiveness for the treatment of refractory wounds. In light of the angiogenic, regenerative characteristics of SVF, it was incorporated as a treatment option in two cases of rectovaginal fistula discussed here. As described in this report, irradiated rectovaginal fistulas in rectal cancer patients were successfully treated with SVF injection, and we suggest SVF as a feasible treatment option for cases of rectovaginal fistula that would otherwise be very difficult to cure.

• IMMUNE NETWORK
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• 제20권5호
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• pp.37.1-37.15
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• 2020

Mycobacterium tuberculosis (Mtb) is an etiologic pathogen of human tuberculosis (TB), a serious infectious disease with high morbidity and mortality. In addition, the threat of drug resistance in anti-TB therapy is of global concern. Despite this, it remains urgent to research for understanding the molecular nature of dynamic interactions between host and pathogens during TB infection. While Mtb evasion from phagolysosomal acidification is a well-known virulence mechanism, the molecular events to promote intracellular parasitism remains elusive. To combat intracellular Mtb infection, several defensive processes, including autophagy and apoptosis, are activated. In addition, Mtb-ingested phagocytes trigger inflammation, and undergo necrotic cell death, potentially harmful responses in case of uncontrolled pathological condition. In this review, we focus on Mtb evasion from phagosomal acidification, and Mtb interaction with host autophagy, apoptosis, and necrosis. Elucidation of the molecular dialogue will shed light on Mtb pathogenesis, host defense, and development of new paradigms of therapeutics.

• 대한물리치료과학회지
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• 제8권2호
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• pp.1005-1013
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• 2001

The present study was designed to investigate the effect of low power GaAlAs laser on spinal Fos expression related to the anti-nociceptive effect of laser stimulation. Low power GaAlAs laser was applied to either acupoint or non-acupoint for 2 hour under light inhalation anesthesia. Spinal Fos expression in the dorsal horn was compared to that obtained in inhalation anesthesia control group. Furthermore, we analyzed the effect of the local treatment of lidocaine on the spinal Fos expression evoked by low power GaAlAs laser stimulation. The results were summarized as follows: 1. In the normal animals, only a few Fos like immunoreactive(Fos-IR) neurons were evident in the lumbar spinal cord dorsal horn. Similarly, following prolonged inhalation anesthesia, Fos-IR neurons were absent in the dorsal horn of the lumbar spinal cord. In animals treated with laser stimulation, Fos immunoreactive neurons were increased mainly in the medial half of ipsilateral laminae I-III at lumbar segments L3-5. These findings directly indicated that prolonged anesthesia used in this study did not affect the Fos expression in the spinal cord dorsal horn of intact animals and low power laser stimulation dramatically produced Fos expression in the spinal cord laminae that are related to the anti-nociceptive effect of laser stimulation. 2. In acupoint stimulated animals, 10mW of laser stimulation, not 3mW and 6mW intensity, significantly increased the number of Fos immunoreactive neurons in the spinal dorsal horn(p<0.05). However, laser stimulation on acupoint more dramatically increased the number of Fos immunoreactive neurons in the spinal cord rather than laser stimulatin on non acupoint. These result suggested that laser stimulatin on acupoint was more effective treatment to activate the spinal neuron than non acupoint stimulation. 3. The local treatment of lidocaine totally suppressed the activity of spinal neurons that were induced by lower power 1aser stimulation. These data indicated that the anti-nociceptive effect of laser stimulation was absolutely dependent upon the peripheral nerve activity in the stimulated location. In conclusion, these data indicate that 10mW of low power laser stimulation into acupoint is capable of inducing the spinal Fos expression in the dorsal horn related to the anti-nociceptive effect of laser stimulation, Furthermore, the induction of spinal Fos expression was totally related to the peripheral nerve activity in the laser stimulated area.

• 공업화학
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• 제32권3호
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• pp.312-319
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• 2021

광열 치료(photothermal therapy)란 빛을 조사하여 열을 발생시킴으로써 정상세포보다 열에 약한 비정상 세포, 특히 암세포를 선택적으로 괴사시키는 치료법이다. 본 연구에서는 광열 치료를 위한 카르복실화된 환원 그래핀옥사이드(reduced graphene oxide with carboxyl groups, CRGO)-골드나노막대(gold nanorod, AuNR) 나노복합체를 합성하고자 하였다. 이를 위해 그래핀옥사이드(graphene oxide, GO)를 고온에서 선택적으로 환원, 박리하여 CRGO를 합성하였고, AgNO₃의 양에 따라 AuNR의 길이를 조절하여 880 nm에서 강한 흡광 특성을 나타내는 AuNR를 합성하여 광열 인자로 사용하였다. 일반적인 방법으로 환원된 RGO에 비해 CRGO에 상대적으로 많은 카르복실기가 결합되어 있음을 FT-IR, 열 중량 분석 및 형광 분석을 통해 확인하였다. 또한, RGO에 비해 많은 carboxyl group이 결합된 CRGO는 수용액상에서 우수한 안정성을 나타내었다. 정전기적 상호작용을 통해 합성된 CRGO-AuNR 나노복합체는 약 317 nm의 균일한 크기와 좁은 크기 분포를 보였다. CRGO-AuNR 나노복합체는 두 가지 광열 인자인 CRGO와 AuNR의 synergistic effect로 인하여 조직 투과도가 우수한 근적외선 880 nm 레이저의 조사에 의한 광열 효과가 AuNR보다 2배 이상 향상 되는 것을 확인하였다. 또한, 광열 효과에 의한 암세포 독성 분석 결과, CRGO-AuNR 나노복합체가 가장 우수한 세포 독성 특성을 나타내었다. 따라서 CRGO-AuNR 나노복합체는 안정된 분산성과 향상된 광열 효과를 기반으로 항암 광열 요법 분야에 응용될 수 있을 것으로 기대된다.

• 대한임상검사과학회지
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• 제56권1호
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• pp.52-58
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• 2024

광역학 요법(photodynamic therapy)은 특정 파장의 빛에 의해 활성화되는 광민감제(photosensitizer)를 사용하여 세포 내 산소를 활성화시키는 치료 방법으로, 항생제 내성균에 의한 상처 감염의 치료에 유망한 접근 방법이다. 일반적으로 건강한 사람에게 비병원성인 녹농균(Pseudomonas aeruginosa, P. aeruginosa)은 특정 병원성 징후를 보이지 않지만, 피부 손상이나 면역력이 저하된 사람들에서는 패혈증과 같은 심각한 질병을 유발할 수 있다. 항생제는 P. aeruginosa 감염에 대한 전통적인 치료법이나 약물 오용으로 인한 항생제 내성의 증가는 이러한 감염을 관리하는 데 큰 어려움을 준다. 본 연구에서는 P. aeruginosa의 억제제로서 광민감제(PhotoMed, Methyl pheophorbide A, Radachlorin^®)와 다이오드 레이저를 이용한 광역학 치료 효과를 조사하는 것을 목표로 했다. P. aeruginosa 현탁액과 광민감제(PhotoMed, Methyl pheophorbide A, Radachlorin^®)를 페트리 접시에 접종하여 30분 후 다이오드 레이저를 사용하여 3 J/cm²의 에너지 밀도로 조사했다. 그 결과, P. aeruginosa는 PhotoMed에서 79.65%, Methyl pheophorbide A에서 47.36%, Radachlorin^®에서 40.91%의 사멸률을 보였다. 이번 연구는 P. aeruginosa를 억제하기 위한 가장 효과적인 접근법이 PhotoMed와 다이오드 레이저를 이용한 광역학 치료임을 보여준다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3903-3907
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• 2013

Background: Patients with colorectal cancer are usually treated with chemotherapy, which reduces the number of blood cells, especially white blood cells, and consequently increases the risk of infections. Some research studies have reported that aromatherapy massage affects the immune system and improves immune function by, for example, increasing the numbers of natural killer cells and peripheral blood lymphocytes. However, there has been no report of any study which provided good evidence as to whether aromatherapy with Thai massage could improve the immune system in patients with colorectal cancer. The objectives of this study were to determine whether the use of aromatherapy with light Thai massage in patients with colorectal cancer, who have received chemotherapy, can result in improvement of the cellular immunity and reduce the severity of the common symptoms of side effects. Materials and Methods: Sixty-six patients with colorectal cancer in Phichit Hospital, Thailand, were enrolled in a single-blind, randomised-controlled trial. The intervention consisted of three massage sessions with ginger and coconut oil over a 1-week period. The control group received standard supportive care only. Assessments were conducted at pre-assessment and at the end of one week of massage or standard care. Changes from pre-assessment to the end of treatment were measured in terms of white blood cells, neutrophils, lymphocytes, CD4 and CD8 cells and the CD4/CD8 ratio and also the severity of self-rated symptom scores. Results: The main finding was that after adjusting for pre-assessment values the mean lymphocyte count at the post-assessment was significantly higher (P=0.04) in the treatment group than in the controls. The size of this difference suggested that aromatherapy with Thai massage could boost lymphocyte numbers by 11%. The secondary outcomes were that at the post assessment the symptom severity scores for fatigue, presenting symptom, pain and stress were significantly lower in the massage group than in the standard care controls. Conclusions: Aromatherapy with light Thai massage can be beneficial for the immune systems of cancer patients who are undergoing chemotherapy by increasing the number of lymphocytes and can help to reduce the severity of common symptoms.

• IMMUNE NETWORK
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• 제12권1호
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• pp.33-39
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• 2012

Background: Therapeutic approaches using monoclonal antibodies (mAbs) against complement regulatory proteins (CRPs:i.e.,CD46,CD55 and CD59) have been reported for adjuvant cancer therapy. In this study, we generated a recombinant 1E8 single-chain anti-CD59 antibody (scFv-Fc) and tested anti-cancer effect.by using complement dependent cytotoxicity (CDC). Methods: We isolated mRNA from 1E8 hybridoma cells and amplified the variable regions of the heavy chain (VH) and light chain (VL) genes using reversetranscriptase polymerase chain reaction (RT-PCR). Using a linker, the amplified sequences for the heavy and light chains were each connected to the sequence for a single polypeptide chain that was designed to be expressed. The VL and VH fragments were cloned into the pOptiVEC-TOPO vector that contained the human CH2-CH3 fragment. Then, 293T cells were transfected with the 1E8 single-chain Fv-Fc (scFv-Fc) constructs. CD59 expression was evaluated in the prostate cancer cell lines using flow cytometry. The enhancement of CDC effect by mouse 1E8 and 1E8 scFv-Fc were evaluated using a cytotoxicity assay. Results: The scFv-Fc constructs were expressed by the transfected 293T cells and secreted into the culture medium. The immunoreactivity of the secreted scFv-Fc construct was similar to that of the mouse 1E8 for CCRF-CEM cells. The molecular masses of 1E8 scFv-Fc were about 120 kDa and 55 kDa under reducing and non-reducing conditions, respectively. The DNA sequence of 1E8 scFv-Fc was obtained and presented. CD59 was highly expressed by the prostate cancer cell line. The recombinant 1E8 scFv-Fc mAb revealed significantly enhanced CDC effect similar with mouse 1E8 for prostate cancer cells. Conclusion: A 1E8 scFv-Fc construct for adjuvant cancer therapy was developed.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제30권6호
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• pp.509-515
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• 2004

광역동치료(PDT)는 갖가지 고형 종양의 치료를 위한 임상적시도로 시작되어 화학요법 및 방사선 요법에 내성이 있는 종양의 대체 치료법의 하나로 제시되고 있다. PDT는 전신적 또는 국소적으로 투여하여 유해한 조직에 선택적으로 농축되도록 한 광증감제와 이를 활성화하는 적절한 파장과 에너지를 가진 레이저광의 조합에 기초한다. 이 연구에서는, 인체 골육종 세포(HOS)에 미치는 레이저 EIT 21의 광독성 효과에 대해 알아보았으며, 그런 광독성 효과가 세포고사를 유발하는가를 규명하고자 하였다. 본 연구는 레이저 EIT 21이 HOS 세포에 대해 광독성을 효과를 가진다는 것을 증명했다. 세포 죽음이 세포괴사에 의해 유발되는지 아니면 세포고사에 의해 유발되는지를 알아보기 위해 세포 고사를 평가 하는 여러 실험 기법을 이용하였다. TUNEL 분석은 극소수만이 응축된 핵의 양성반응을 보여주었다. Hemacolor와 AO/EB 염색 또한 대부분의 세포가 괴사로 죽는 것을 보여주었다. 레이저 EIT로 조사된 HOS 세포에서 응축되거나 분절된 핵을 발견하는 것은 어려웠다. DNA 전기영동에서, 세포고사에서 보여지는 DNA 분절의 전형적인 특징인 사다리형 절편 형태(ladder fragmentation pattern)가 나타나지 않았다. Western blotting에 의한 분석에서 p53의 발현은 일정하게 나타났고 레이저로 조사된 세포는 caspase-3과 PARP의 분열을 나타내지 않는 것으로 보아 레이저 유도 세포 죽음(laser-induced cell death)은 p53과는 관련이 없는 것 같다.