• Title/Summary/Keyword: light stress

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Comparison of the responses of two Dunaliella strains, Dunaliella salina CCAP 19/18 and Dunaliella bardawil to light intensity with special emphasis on carotenogenesis

  • Park, Seunghye;Lee, Yew;Jin, EonSeon
    • ALGAE
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    • v.28 no.2
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    • pp.203-211
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    • 2013
  • Dunaliella salina and Dunaliella bardawil are well known for carotenogenesis, the overproduction of carotenoids, under stress conditions. The effect of high light (HL) and low light (LL) on the growth, morphology, photosynthetic efficiency, and the ${\beta}$-carotene and zeaxanthin production of D. salina CCAP 19/18 and D. bardawil was investigated and compared. Both strains showed similar growth kinetics under LL growth condition, but D. salina CCAP 19/18 was faster. As the light intensity increased, D. salina CCAP 19/18 cells were elongated and D. bardawil cells became larger. Both strains showed decrease of the maximum quantum yield of PSII ($F_v/F_m$) and election transport rate (ETR) under HL growth condition and D. salina CCAP 19/18 was less liable to the light stress. Both strains had about 1.8 and 5 times difference in the $O_2$ evolution rate at LL and HL conditions, respectively. The ${\beta}$-carotene and zeaxanthin production were increased as the light intensity increased in both strains. D. bardawil was more sensitive to light intensity than D. salina CCAP 19/18. The possible application of D. salina CCAP 19/18 as a carotenogenic strain will be discussed.

A Study of the Acclerated Degradation Phenomena on th Amorphous Silicon Thin Film Transistors with Multiple Stress (복합 스트레스에 의한 비정질 실리콘 박막 트랜지스터에서의 가속열화 현상 연구)

  • 이성규;오창호;김용상;박진석;한민구
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.43 no.7
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    • pp.1121-1127
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    • 1994
  • The accelerated degradation phenomena in amorphous silicon thin film transistors due to both electrical stress and visible light illumination under the elevated temperature have been investigated systematically as a function of gate bias, light intensity, and stress time. It has been found that, in case of electrical stress, the thrshold voltage shifts of a-Si:H TFT's may be attributed to the defect creation process at the early stage, while the charge trapping phenomena may be dominant when the stressing periods exceed about 2 hours. It has been also observed that the degradation in the device characteristics of a-Si:H TFT's is accelerated due to multiple stress effects, where the defect creation mechanism may be more responsible for the degradation rather than the charge trapping mechanism.

Identification and Isolation of Differentially Expressed Gene in Response to Cold Stress in a Green Alga, Spirogyra varians (Zygnematales)

  • Han, Jong-Won;Yoon, Min-Chul;Lee, Key-Pyoung;Kim, Gwang-Hoon
    • ALGAE
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    • v.22 no.2
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    • pp.131-139
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    • 2007
  • The expression of genes responding to cold stress in a freshwater alga, Spirogyra varians, was studied by using differential expression gene (DEG) method. A gene strongly up-regulated in 4°C was isolated and designated as SVCR2 (Spirogyra varians cold regulated) gene. The cDNA encoding SVCR2 was cloned using λZAP cDNA library of Spirogyra varians. The deduced amino acid had a sequence similarity with trans-membrane protein in Arabidopsis thaliana (Q9M2D2, 52.7%). Northern blot analysis demonstrated that transcript level of SVCR2 increased about 10 fold under low temperature (4°C), compared with that cultured at warm (20°C) conditions. The expression of SVCR2 was also affected by light conditions. When the plants were exposed to high light (HL) (1200 μmol photon m–2 s–1), the expression of SVCR2 began within 2 hrs. This gene expression lasted for 4 hrs and decreased afterwards. Under the blue light (470 nm) condition, the expression of this gene was induced in same way as HL treatment, even under less than 100 μmol photon m–2 s–1. But red light (650 nm) and UV-A irradiation did not affect the expression of SVCR2.

Anaysis of the photoelastic of CR lens using circular polariscope (원편광기를 이용한 CR 렌즈의 광 탄성 해석 연구)

  • Kim, Yong-Geun
    • Journal of Korean Ophthalmic Optics Society
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    • v.6 no.2
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    • pp.11-16
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    • 2001
  • The polariscope to measure :he stress in lens was made up quarter-wave plate polarizer and we analyzed two components of light's wave $E_1$ and $E_2$ following the steps. It is clear that the principal-stress difference ${\sigma}_1-{\sigma}_2$ can be determined in 2-D model if fringe order N is measured each point in sample moreover. the optical axes of sample coincide with the principal-stress directions. The birefringence acted to a light wave and a phase retardation were in proportioned to the principal-stressed difference (${\sigma}_1-{\sigma}_2$) and the intensity of final light wave was proportioned to $sin^2({\Delta}/2)$, when ${\Delta}/2=n^{\pi}$ (n=0, 1, 2, ...) and the extinction occurs. As a experimental result, the extinction band shifted owing to a magnitude of lens' external stress.

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Light Effects on the Bias Stability of Transparent ZnO Thin Film Transistors

  • Shin, Jae-Heon;Lee, Ji-Su;Hwang, Chi-Sun;KoPark, Sang-Hee;Cheong, Woo-Seok;Ryu, Min-Ki;Byun, Chun-Won;Lee, Jeong-Ik;Chu, Hye-Yong
    • ETRI Journal
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    • v.31 no.1
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    • pp.62-64
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    • 2009
  • We report on the bias stability characteristics of transparent ZnO thin film transistors (TFTs) under visible light illumination. The transfer curve shows virtually no change under positive gate bias stress with light illumination, while it shows dramatic negative shifts under negative gate bias stress. The major mechanism of the bias stability under visible illumination of our ZnO TFTs is thought to be the charge trapping of photo-generated holes at the gate insulator and/or insulator/channel interface.

Structural Optimization of a Light-weight Manhole Cover Using FEM and Response Surface Method (유한요소법과 반응표면법을 이용한 경량 맨홀 커버 구조 최적화 설계)

  • Lee, Hyoungwook
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.25 no.6
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    • pp.462-470
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    • 2016
  • The locking load of a conventional manhole depends on the weight of its cover. Locking-type manhole structures with a special locking mechanism were recently developed to prevent accidents such as stolen cover, away cover from a frame. The weight of the manhole structure can be reduced under structural safety because the locking force of a locking-type manhole is greater than the weight of the cover. A light-weight manhole cover is developed in this study by using a finite element stress analysis and the design of experiments. Static stress analysis and fracture experiments are also conducted to analyze the states of the initial product. The optimum light-weight manhole cover considering manufacturing molds is developed and tested. Consequently, the weight was found to reduce by 16%. In addition, the fracture load increased by 38%.

Transcriptome analysis of Panax ginseng response to high light stress

  • Jung, Je Hyeong;Kim, Ho-Youn;Kim, Hyoung Seok;Jung, Sang Hoon
    • Journal of Ginseng Research
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    • v.44 no.2
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    • pp.312-320
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    • 2020
  • Background: Ginseng (Panax ginseng Meyer) is an essential source of pharmaceuticals and functional foods. Ginseng productivity has been compromised by high light (HL) stress, which is one of the major abiotic stresses during the ginseng cultivation period. The genetic improvement for HL tolerance in ginseng could be facilitated by analyzing its genetic and molecular characteristics associated with HL stress. Methods: Genome-wide analysis of gene expression was performed under HL and recovery conditions in 1-year-old Korean ginseng (P. ginseng cv. Chunpoong) using the Illumina HiSeq platform. After de novo assembly of transcripts, we performed expression profiling and identified differentially expressed genes (DEGs). Furthermore, putative functions of identified DEGs were explored using Gene Ontology terms and Kyoto Encyclopedia of Genes and Genome pathway enrichment analysis. Results: A total of 438 highly expressed DEGs in response to HL stress were identified and selected from 29,184 representative transcripts. Among the DEGs, 326 and 114 transcripts were upregulated and downregulated, respectively. Based on the functional analysis, most upregulated and a significant number of downregulated transcripts were related to stress responses and cellular metabolic processes, respectively. Conclusion: Transcriptome profiling could be a strategy to comprehensively elucidate the genetic and molecular mechanisms of HL tolerance and susceptibility. This study would provide a foundation for developing breeding and metabolic engineering strategies to improve the environmental stress tolerance of ginseng.

A Study on the Lightweight Design of a Cross Beam for Railway Passenger Coach (철도객차용 크로스 빔의 경량화 설계에 관한 연구)

  • Jang, Deuk-Yul;Jeon, Hyung-Yong
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.16 no.5
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    • pp.126-133
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    • 2017
  • This report investigates the stress distribution according to the location and shape change of the circular hole for the lightweight design of the cross beam of a railway passenger car and studies the lightweight design. To design a lightweight cross beam with a circular hole, we selected the non-circular crossbeam as a basic model, examined the stress distribution and displacement by position and determined the location, shape, size and quantity of the hole for light weight. We analyzed the effects of the position and shape of the hole on the maximum equivalent stress and displacement. The influencing factors were set as the design parameters, and the stress value was examined according to the variation of each variable. By considering the stress value according to the change of each variable and selecting the design parameter with the narrowest scattering value of the stress at each position of the hollow cross beam with various hole positions and shapes, we studied a cross beam with a circle hole under identical load condition to have an equal stress distribution to that of a non-circular cross beam.

Dynamic Quasi-Elastic Light Scattering Measurement of Biological Tissue

  • Youn, Jong-In;Lim, Do-Hyung
    • Journal of Biomedical Engineering Research
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    • v.28 no.2
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    • pp.169-173
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    • 2007
  • During laser irradiation, mechanically deformed cartilage undergoes a temperature dependent phase transformation resulting in accelerated stress relaxation. Clinically, laser-assisted cartilage reshaping may be used to recreate the underlying cartilaginous framework in structures such as ear, larynx, trachea, and nose. Therefore, research and identification of the biophysical transformations in cartilage accompanying laser heating are valuable to identify critical laser dosimetry and phase transformation of cartilage for many clinical applications. quasi-elastic light scattering was investigated using Ho : YAG laser $(\lambda=2.12{\mu}m\;;\;t_p\sim450{\mu}s)$ and Nd:YAG Laser $(\lambda=1.32{\mu}m\;;\;t_p\sim700{\mu}s)$ for heating sources and He : Ne $(\lambda=632.8nm)$ laser, high-power diode pumped laser $(\lambda=532nm)$, and Ti : $Al_2O_3$ femtosecond laser $(\lambda=850nm)$ for light scattering sources. A spectrometer and infrared radiometric sensor were used to monitor the backscattered light spectrum and transient temperature changes from cartilage following laser irradiation. Analysis of the optical, thermal, and quasi-elastic light scattering properties may indicate internal dynamics of proteoglycan movement within the cartilage framework during laser irradiation.

A Study on the Applicability of Shrinkage Reduction Effect of Light-weight Aggregate Concrete (경량골재 콘크리트의 수축 저감효과에 관한 적용성 연구)

  • Lim, Sang-Jun;Bang, Chang-Joon;Park, Jong-Hyok
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2011.11a
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    • pp.175-176
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    • 2011
  • Applying previous studies performed in the moisture transportation characteristics and shrinkage of lightweight concrete application of shrinkage reduction is to discuss. Applicability of shrinkage reduction effect of lightweight concrete applies for the analysis of PSC girder bridge beam placed on the construction site. Stress of the concrete bridge deck, rebar quantity is calculated by effective elastic modulus method and crack risk is assessed by moisture transport and differential shrinkage analysis. After approximately 10 days maximum tensile stress occurs 6MPa, similar to the case of normal concrete, a maximum tensile stress occurs 3MPa in lightweight concrete and comparing to normal concrete stress was reduced to approximately 50%. Normal and lightweight concrete crack index, respectively, is reduced 1.6 to 1.2, 1.2 to 0.9 in surface and boundary region. Therefore, reduction in shrinkage of concrete were able to confirm reduction of crack risk.

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