• 제목/요약/키워드: light extraction

검색결과 474건 처리시간 0.031초

Salinibacter ruber로부터 잔토로돕신의 분리와 in vitro에서 재구축 (Separation of Xanthorhodopsin from Salinibacter ruber and Its in vitro Reconstruction)

  • 공민경;임정한;이평천
    • 청정기술
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    • 제17권3호
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    • pp.280-282
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    • 2011
  • 광에너지를 이용하여 바이오원료 및 바이오케미컬을 생산하려는 새로운 시도가 많은 관심을 받고 있다. 특히, 광합성기작을 이용한 다양한 바이오유래 물질을 생산하려는 연구가 진행되고 있다. 본 연구에서는 광합성기작을 갖고 있는 해양 미생물인 Salinibacter ruber의 세포막에서 retinal과 salinixanthin의 작용으로 광전자를 흡수하는 역할을 하는 잔토로돕신을 수성 이상계 계면 농축법을 이용하여 효율적으로 분리하였다. 분리된 잔토로돕신을 생물학적 세포막인 레시틴으로 리포좀을 생성하여 잔토로돕신의 광전자흡수 활성을 그대로 갖는 인위적인 잔토로돕신-레시틴 리포좀을 제작하였다.

Roasting and Cryogenic Grinding Enhance the Antioxidant Property of Sword Beans (Canavalia gladiata)

  • Jung, Ju-Yeong;Rhee, Jin-Kyu
    • Journal of Microbiology and Biotechnology
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    • 제30권11호
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    • pp.1706-1719
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    • 2020
  • The objective of this study was to optimize the conditions for enhancing the antioxidant properties of sword bean (Canavalia gladiata) as a coffee substitute in two processing methods, roasting and grinding. The optimum conditions for removing off-flavor of the bean and maximizing functionality and efficiency were light roasting and cryogenic grinding (< 53 ㎛). In these conditions, extraction yield was 16.75%, total phenolic content (TPC) was 69.82 ± 0.35 mg gallic acid equivalents/g, and total flavonoid content (TFC) was 168.81 ± 1.64 mg quercetin equivalents/100 g. The antioxidant properties were 77.58 ± 0.27% for DPPH radical scavenging activity and 58.02 ± 0.76 mg Trolox equivalents/g for ABTS radical scavenging activity. The values for TFC and ABTS radical scavenging activity were significantly higher (p < 0.05) than in other conditions, and TPC and DPPH radical scavenging activity were second highest in lightly roasted beans, following raw beans. HS-SPME/GC-MS analysis confirmed that the amino acids and carbohydrates, which are the main components of sword bean, were condensed into other volatile flavor compounds, such as derivatives of furan, pyrazine, and pyrrole during roasting. Roasted and cryogenically ground (cryo-ground) sword beans showed higher functionality in terms of TFC, DPPH, and ABTS radical scavenging activities compared to those of coffee. Overall results showed that light roasting and cryogenic grinding are the most suitable processing conditions for enhancing the bioactivity of sword beans.

스마트 폰 어플리케이션 적용을 위한 UPC-A Bar code 인식 알고리즘 개발 (Development of the UPC-A Barcode Recognition Algorithm for Smartphone Applications)

  • 이상준;이상용;이영범;이명호
    • 전기학회논문지
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    • 제60권1호
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    • pp.174-183
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    • 2011
  • This paper is about a bar code decoding algorithm developed for smart phone applications. The algorithm consists of bar code data extraction procedure, bar code signal estimation procedure, and bar code decoding procedure. To detect the peak bar code module, a DSTW had been applied because of its outstanding performance in ECG peak detection. In order to minimize errors due to non-uniform light effect, the proposed algorithm was acted as a baseline wandering filter based on module peaks detection. The algorithm had been tested to evaluate the performance under the conditions of blurring, non-uniformed light and white noises. The algorithm had shown excellent performance in reconstruction of bar code decoding, compared to other conventional methods. In order to show the possibility of applying the algorithm to a smart phone, a real UPC-A type 150 bar code pictures obtained from a smart phone camera was applied to the algorithm, achieving the correct recognition rate of 97.33%.

Thickness Measurement of a Transparent Thin Film Using Phase Change in White-Light Phase-Shift Interferometry

  • Kim, Jaeho;Kim, Kwangrak;Pahk, Heui Jae
    • Current Optics and Photonics
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    • 제1권5호
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    • pp.505-513
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    • 2017
  • Measuring the thickness of thin films is strongly required in the display industry. In recent years, as the size of a pattern has become smaller, the substrate has become larger. Consequently, measuring the thickness of the thin film over a wide area with low spatial sampling size has become a key technique of manufacturing-yield management. Interferometry is a well-known metrology technique that offers low spatial sampling size and the ability to measure a wide area; however, there are some limitations in measuring the thickness of the thin film. This paper proposes a method to calculate the thickness of the thin film in the following two steps: first, pre-estimation of the thickness with the phase at the peak position of the interferogram at the bottom surface of the thin film, using white-light phase-shift interferometry; second, accurate correction of the measurement by fitting the interferogram with the theoretical pattern through the estimated thickness. Feasibility and accuracy of the method has been verified by comparing measured values of photoresist pattern samples, manufactured with the halftone display process, to those measured by AFM. As a result, an area of $880{\times}640$ pixels could be measured in 3 seconds, with a measurement error of less than 12%.

꽃사과(Malus prunifolia Wild. Borkh. "Red Fruit")에서 에탄올 추출한 안토시안 색소의 안정성 (Stabilities of Anthocyanin Pigmenta obtained from Crab Apple (Malus prunifolia Wild. Borkh. "Red Fruit") by Ethanol Extraction)

  • 김용환
    • 한국식품영양학회지
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    • 제12권1호
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    • pp.85-90
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    • 1999
  • The characcteristics of anthocyanin pigments from crab apple (Malus prunifolia Wild. Borkh. "red fruit") by ethanol extract were investigated at various condition of light temperature sugar, organic acid me-tal ion and pH. The pigments were stable(over the 60%) on the light irradiation throughout 20 days sto-rage period at room temperature and in the pesenc of Al-foil red blue green and yellow cover were rage period at room temperature and in the pesence of Al-foil red blue green and yellow cover were very stable. The pigments also showed high thermal stbility(over the 67% at 115$^{\circ}C$ 10min) at pH2.5 respectively. The pigments with added organic acid greatly increased thickness of red color. The pig-ments with added metal ions at pH 2.5 such as Na+ K+, Mg2+ Ca2+ and Mn2+ were stable throughout 20 days storage period at $25^{\circ}C$. But Cu2+ addition showed the rapidly degradation of the pigments and Al3+ addition induced the color conversion from red to redish violet. The thickness of the red color of anthocyanin pigments increased increased as the pH decreased. These results indicated that crab apple antho-cyanin pigments might be potental source of natural food colorant. colorant.

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손금과 정맥혈관 패턴매칭을 이용한 비접촉 출입 보안시스템 설계 (Design of a Contactless Access Security System using Palm Creases and Palm Vein Pattern Matching)

  • 김기중
    • 한국전자통신학회논문지
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    • 제19권1호
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    • pp.327-334
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    • 2024
  • 본 논문에서는 라즈베리파이 기반으로 손바닥 정맥혈관 이미지를 획득하기 위하여 950nm파장을 가지는 근적외선 LED 광원 장치와 손금을 획득하기 위한 백색 LED 광원 장치를 가지는 시스템을 개발하였다. 또한 획득한 정맥 및 손금 이미지에 대하여 영상 전처리 과정(흑백화, 평활화, 이진화, 블러링, 세선화 등)을 통하여 정맥과 손금이 혼합된 위조 방지 및 보안이 강화된 고유 패턴이 추출 가능한 영상처리 기술을 구현하여 보안성이 강화된 시스템에서 활용할 수 있는 원천 기술을 확보하였다.

정어리 지질의 추출속도에 관한 연구 (Studies on the Extraction Rate of Oil from Sardine, Sardinops melanosticta)

  • 양현석;이근태;변대석
    • 한국수산과학회지
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    • 제17권1호
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    • pp.40-46
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    • 1984
  • 용매추출법에 의한 정어리 백색육중의 지질을 hexane으로 추출하여 추출속도 이론과의 연관성을 구명하고 또한 추출속도에 미치는 인자중 온도 및 수분함량의 영향을 검토한 결과는 다음과 같다. 1. 정어리 지질의 추출은 초기와 후기로 나누어지며 율속단계인 후기에서의 확산계수를 구하기 위하여 Fick의 비정상 상태 확산이론을 적용시키는 것은 가능하다. 2. $30^{\circ}C,\;45^{\circ}C,\;60^{\circ}C$의 추출온도에서 이론과의 연관성이 가장 좋았던 것은 $30^{\circ}C$의 경우였으며 $45^{\circ}C,\;60^{\circ}C$의 경우에서는 이론보다 빠르게 진행되었으나 오차의 범위내에 있었다. 3. 추출속도의 온도의존성은 추출곡선의 기울기 (K)에 대하여 절대온도(T)의 11승에 비례하여 증가하였다. 4. 수분함량이 추출속도에 미치는 영향은 $32.42\%$에서 적을수록 추출속도는 빨랐으며. 추출곡선의 기울기(K)는 수분함량 $1\%$의 감소에 대략 $0.53{\times}10^{-6}cm^2/sec$씩 증가하였다.

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Streptococcus mutans에 대한 생강과 천연추출물의 광역학 항균효과 (Antibacterial effect of Zingiberaceae extracts mediated photodynamic therapy on Streptococcus mutans)

  • 황혜림;강시묵;이은송;김백일
    • 대한치과의사협회지
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    • 제57권10호
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    • pp.560-568
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    • 2019
  • Purpose: This study evaluated the antibacterial effects of curcuma, ginger, and finger root extracts in water-soluble powder on planktonic Streptococcus mutans(S.mutans), which is known to cause dental caries, in order to confirm whether these extracts could perform as photosensitizers for the effects of photodynamic therapy (PDT). Methods: This study used the strain of streptococcus mutans ATCC 25175 distributed by the Korean Collection for Type Cultures of the Korea Research Institute of Bioscience & Biotechnology. Commercial edible curcuma, ginger and finger root were used as the natural extracts for the use of photosensitizer. To extract organic solvent, 3 g of each powder was mixed in $30m{\ell}$ of dimethyl sulfoxide (DMSO, VWR, Germany) before extraction. $1.8m{\ell}$ of the photosensitizer solution, manufactured in the concentrations of 5, 0.5, and $0.05mg/m{\ell}$, was mixed with $0.2m{\ell}$ of the S. mutans culture medium that had been cultured for 2 days. To induce the photodynamic reaction, Qraycam (AIOBIO, Seoul, Korea) equipped with 405 nm LED was used to expose light for 5 minutes to irradiate 59 nW energy for 300 seconds. Results: Compared with the case with no light, a higher photodynamic therapeutic effect was confirmed with $0.05mg/m{\ell}$ curcuma powder extract, the concentration of $0.5mg/m{\ell}$ and LED light of 405 nm wavelength (p=0.000, p=0.003). $0.05mg/m{\ell}$ of curcuma powder extract and the concentration of $0.5mg/m{\ell}$ showed 100% antibacterial effect when exposed to light, whereas the concentration of $5mg/m{\ell}$ showed 11.95% antibacterial effect. When exposed to light, $0.05mg/m{\ell}$ of ginger powder extract showed an antibacterial effect which didn't statistically decrease. The concentrations of $0.5mg/m{\ell}$ and $5mg/m{\ell}$ did not show any antibacterial effects. As a result of examining any photodynamic therapeutic effects of finger root powder extract on S. mutans, no statistically significant effect was found. Conclusion: The curcuma powder extract is expected to perform as a photosensitizer. Even though belonging to the same ginger family, ginger powder and finger root powder seem difficult to perform as photosensitizer.

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이동객체의 메타데이터 필터링을 이용한 관심객체 추출 시스템 설계 (The Design of Object-of-Interest Extraction System Utilizing Metadata Filtering from Moving Object)

  • 김태우;김형헌;김평강
    • 정보과학회 논문지
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    • 제43권12호
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    • pp.1351-1355
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    • 2016
  • 매년 증가하는 CCTV와 이를 효율적으로 관제하기 위한 지능형 영상 시스템에 대한 수요가 계속적으로 증가하고 있다. 그럼에도 불구하고 기존 영상분석엔진은 구동을 위해 매우 높은 사양을 요구할 뿐만 아니라 정확한 탐지율도 담보하지 못하는 실정이다. 본 논문에서는 가벼운 영상 분석기법을 적용해 이동 객체의 위치, 크기, 영상 내 존재 시간과 같은 기본적인 메타를 생성하고 이에 대한 데이터 분석을 통해 관심 객체를 찾아내는 연구를 수행하였다. 그 결과, 가벼운 영상분석 알고리즘 결과의 심층적인 데이터 분석을 통해 가벼운 알고리즘이 수반하는 상당량의 노이즈를 제거하고 관심 객체를 효과적으로 추출할 수 있음을 확인하였다. 본 연구 결과는 향후 지능형 기반 능동적 관제시스템 개발에 기여할 것으로 기대한다.

빛 보상과 외형 기반의 특징을 이용한 얼굴 특징 검출 (A Facial Feature Detection using Light Compensation and Appearance-based Features)

  • 김진옥
    • 인터넷정보학회논문지
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    • 제7권3호
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    • pp.143-153
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    • 2006
  • 얼굴 특징 검출은 HCI, 얼굴 인식, 얼굴 추적, 표정 인식 및 이미지 데이터 검색등과 같은 응용분야의 근간 기술이다. 실시간 환경에서 얼굴 특징 검출을 처리하기 위해서는 검출 알고리즘의 속도가 중요한 관건으로 작용하고 있다. 또한 빛의 변화, 대상의 위치, 각도, 복잡한 배경등과 같은 요인들은 얼굴 특징 검출 알고리즘의 검출율을 낮추는데 영향을 미치므로 이를 개선한 방법이 필요하다. 본 연구에서는 검출율과 검출 속도를 동시에 개선한 알고리즘을 제안한다. 제안 알고리즘은 얼굴 이미지에 빛 보상 알고리즘인 CLAHE를 이용하여 빛의 변화에 강건하도록 이미지를 개선한 다음 얼굴 피부 영역을 검출한다. 검출한 피부 영역에서 얼굴 특징 포인트를 추출하기 위해 얼굴 특징의 외형기반 기하학적 성질을 이용한다. 제안 알고리즘은 얼굴 특징 검출의 정확도를 높일 뿐 아니라 빠른 검출 속도를 보임으로써 얼굴 추적, 인식 등과 같은 실시간 응용분야에 적용할 수 있다.

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