• Title/Summary/Keyword: light chilling

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Dormancy and Germination Characteristics of Round-Leaved Sundew (Drosera rotundifolia L.) Seeds Native to Korea (자생 끈끈이주걱(Drosera rotundifolia L.) 종자의 휴면과 발아특성)

  • Cho, Ju Sung;Lee, Cheol Hee
    • Korean Journal of Plant Resources
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    • v.29 no.5
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    • pp.564-573
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    • 2016
  • This research was performed to develop mass propagation method of round-leaved sundew (Drosera rotundifolia L.) for improving horticultural usefulness. Seeds were collected around Hwaseong-si, Gyeonggi-do in October of 2011 and experimented upon while being dry-stored at 4 ± 1.0℃ (darkness). Seed length of a major and minor axis were 1.58 ± 0.060 and 0.21 ± 0.016 ㎜, respecrively, and weight of 1,000 seeds was 6.24 ± 0.172 ㎎. Seeds were thus classified as ‘dwarf seeds’. Regarding the dormancy type, since round-leaved sundew seeds were dormancy broken and germinated at 20~30℃ under the light condition after wet-chilling treatment for 12 weeks, seeds were estimated to have physiological dormancy. Germination conditions of dormancy broken seeds were found to be 20℃ and light condition (54.7%), but germination decreased at higher temperature. Percent germination (PG), germination energy (GE), mean germination time (MGT) and T50 were effectively improved by chemical treatment such as GA3 200 ㎎/L + kinetin 20 ㎎/L and wet-chilling treatment for 14 weeks. In conclusion these optimal conditions were thought to be a useful method for raising seedling uniformly.

Electron Transport Activities of Chloroplasts Isolated from the Detached Rice Leaves Stored under Low Temperature with Illumination (광 및 저온처리한 벼잎 절편에서 분이한 엽록체의 전자전달 활성)

  • 문병용
    • Journal of Plant Biology
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    • v.31 no.4
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    • pp.299-307
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    • 1988
  • The electron transport activities of choloroplasts isolated from hte detached rise (Oryza sativa L. cv. Chucheong) leave stored under low temperature(4$^{\circ}C$) with light illumination were investigated to understand the role of light in the low temperature inhibition of photosynthesis in the chilling-sensitive plants. Chlorophyll content of the detached leaves upon incubation at 28$^{\circ}C$ and 4$^{\circ}C$ in the dark was also measured. The rice seedlings were grown with Hoagland medium in the growth chamber of 28$^{\circ}C$ temperature and 400 ft.c fluorescent light with the photoperiod of 16 h. Although chlorophyll content of the detached leaves stored in the dark declined by 61.7% after 28$^{\circ}C$ treatement, there occurred only 5.2% decrease of chlorophyll with 4$^{\circ}C$ treatment. Low temperature treatment(4$^{\circ}C$) for 6 days brought about decreases in total photosystem(PS II+PS I) activities by 35.2% and 73.6% in te presence and absence of light, respectively, while after 28$^{\circ}C$ treatment of the detached leaves for 6 days in the dark there was only 27.6% decrease in PS II+PS I activity. PS II activities were also decreased by 35.6% and 72.2% in the light and dark, respectively. PS I activities were decreased slightly, however, by 7.6% and 16.2% in the light and dark, respectively. Investigations into DPClongrightarrowDCPIP and NH2OHlongrightarrowDCPIP activities revealed that low temperature inhibition of PS II activities was not due to the inactivation of the water oxidation capacity at low temperature. It was concluded that light protects the electron transport activities of isolated rice chloroplasts from the inhibitory effect of low temperature in the detached leaves.

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Photosynthetic Responses and Photoprotection in Korean Hot Pepper (Capsicum annuum L.) against High Light Stress (광 스트레스에 대한 고추 잎의 광합성 반응과 광 보호 메커니즘)

  • Lee, Hae-Youn;Park, Youn-Il;Kim, Chang-Gi;Hong, Young-Nam
    • Korean Journal of Environmental Agriculture
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    • v.25 no.2
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    • pp.109-117
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    • 2006
  • Photoinhibition and photoprotection of PSII in the leaves of hot pepper (Capsicum annuum L.) grown in Hoagland solution and Tap water were compared. Though changes in the rates of $O_2$ evolution as a function of photon fluence rate (PFR) were comparable, the rates of respiration in the dark was 3 times higher in the Hoagland solution grown leaves than in the Tap-water grown ones. Compared to Hoagland solution grown plane, PSIIs of Tap water grown pepper leaves were more susceptible to photoinhibitory light treatment. In order to inactivate functional PSII to the same extents, Hoagland solution grown plants required almost 2-fold high light $(1600{\mu}molm^{-2}s^-)$ treatment than those of Tap water $(900{\mu}molm^{-2}s^-)$. Interestingly, the remaining fraction of PSII in Hoagland grown pepper was able to survive under prolonged illumination in the presence of lincomycin, which probably means that the growth condition of plant seemed to have an effect on the recovery of PSII from light stress. When PSII was severly photoinactivated at a chilling temperature, recovery was observed only if the residual functional PSII were not inhibited with DCMU, Nigericin and MV during recovery. In conclusion, PSIIs grown in the Hoagland solution was more resistant to excess light than in the Tap water grown one and the recovery of PSII from photodamage was more efficient in Hoagland grown pepper leaves than Tap water grown one, which means that the increased dark respiration may play a important role in the protection of PSII from photoinhibition by helping repair photosynthetic proteins (in particular, the D1 protein of PSII) degraded by photoinhibition.

Dormancy Physiology, softening culture and evaluation of nutrition value in the Ulrung-native Allium victorialis var. platyphyllum (야생 산마늘의 휴면 생리 및 연화 재배)

  • Choi, Sang-Tai;Lee, Joon-Tak;Park, Woo-Churl
    • Applied Biological Chemistry
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    • v.36 no.6
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    • pp.495-501
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    • 1993
  • This experiment was carried out to find out the dormancy physiology, method of softening culture and evaluation of nutritional value of wild garlic, Ulrung-native Allium victorialis var. platyphyllum. In March, a new bulbs, the shoot and bulbs began to develop until the bulbs showed their complete dormant states in late August. The bulbs renewed to another one in every years. When shoots germinated about $1{\sim}2\;cm$ from mother bulbs, the soft tissues in the mother bublbs was degenerated and finally remained as only fiberous tissues unlike the other bulbaceous plants. There was a high inhibiting activities like ABA in the bulbs. This is believed that this inhibiting substance like ABA in the bulbs is related to the dormancy of wild garlic. Although the immatured bulbs, harvested at May and June, was treated with chilling for 90 days, it didn't germinate their shooting, but the matured bulbs, harvested at July and August, could germinate their shooting over 1 cm in 75 and 60 days chilling treatment, respectively. The shoot elongation was promoted by the longer chilling periods, the later harvesting day and the dark condition. The crude fiber content of leaf and stem increased at more expanded leaf and higher light intensity condition. Since the shoots, grown from germinating to leaf expanding time, had a good quality for food stuff and had less crude fibers, we supposed this period is to be most appropriate for harvesting time.

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Effect of Blue Color-deficient Sunlight on the Productivity and Cold Tolerance of Crop Plants (청색파장(靑色波長)영역이 결여된 태양광이 작물(作物)의 생산성(生産性) 및 내냉성(耐冷性)의 향상에 미치는 효과 Ⅰ. 광합성(光合成) 및 호흡(呼吸)의 전자전달계 활성(活性)의 변화)

  • Jung, Jin;Kim, Jong-Bum;Min, Bong-Ki
    • Korean Journal of Environmental Agriculture
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    • v.5 no.2
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    • pp.141-148
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    • 1986
  • The blue-light effect on the grown as well as on the physiological activity of some major horticultural plants in Korea has been investigated. The light quality used for the work was obtained from sunlight filtered by an orangecolored polyethylene film which removed about 70% of visible light in the spectral region of $350㎚{\sim}500㎚$. The film was developed in this laboratory especially for the work and named BCR film meaning blue color-removing film. The light environment in the plastic house which was built with BCR film provided plants with the blue color-deficient sunlight. Thus, the photobiological effect of blue light could be examined conversely by comparing with the effect of white sunlight in a conventional plastic house built with colorless polyethylene film. In a sense of applicability to horticulture, two remarkable effects of the blue color-deficient sunlight on plant physiology were observed: First, it enhanced to a great extent the growth activity of plants-pepper, cucumber, zucchini, tomato, and leaf lettuce at the vegetative stage as well as at the reproductive stage, as demonstrated by their yield which were in average $40{\sim}50%$ increased compared with the control (under white sunlight). Second, it improved significantly the cold tolerance of plants, as exhibited with their resistance to chilling during treatment in a cold chamber maintained at a temperature which caused chilling injury to the plants of control. The visualized effects were reflected on the physiological activity of cells on organelle level. Chloroplast isolated from the plant leaves grown under BCR film showed considerably stronger photosynthetic activity, as judged by the increased electron transport rate of illuminated chloroplast, than that from leaves grown under white PE film. Mitochondria from leaves grown under BCR film maintained normal respiration activity until temperature decreased to a few degree($^{\circ}C$) lower than the temperature which caused respiratory inhibition to mitochondria obtained from leaves of the control.

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Effect of Prechilling, Light Quality and Daily Irradiation Hours on Seed Germination in Three Campanulan Plants (저온처리(低溫處理), 파종후(播種後) 광질(光質) 및 일중조명시간(日中照明時間)에 따른 도라지, 더덕, 만삼의 발아율(發芽率))

  • Kang, Jin-Ho;Park, Jin-Seo;Ryu, Yeong-Seop
    • Korean Journal of Medicinal Crop Science
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    • v.5 no.2
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    • pp.131-138
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    • 1997
  • Campanulaceae having the most growing areas among medicinal crops cultivated in Korea occasionally failed to establish a reasonable standing in practice. The experiment was carried out to examine the effect of prechilling (0 : 4 : 8 days), light quality (red : white : dark) and daily irradiation hours (8 : 12 : 16) after sowing on seed germination and radicle elongation of Campanulaceae (Platycodon grandiflorum : Codonopsis lanceolata : C. pjlosula) to give an information on their earlier standing establishment. Mean germination rate of P. grandiflorum was the highest but that of C. pilosula was the lowest regardless of all the treatments. 12 hours irradiation or prechilling increased to 8 days enhanced their earlier or later germination, respectively. White light increased the rate of P. grandiflorum but alleviated that of C. lanceolata regardless of the daily irradiation hours. Although prechilling eliminated such effect of white light, light quality treatment effect on their mean germination rates was influenced by period after sowing, daily irradiation hours or prechilling. On the 9th day after sowing, C. lanceolata showed the greatest radicle length, and both daily 8 hours irradiation and 8 days prechilling enforced to elongate their radicles, while P. grandjflorum and C. lanceolata more lengthened their radicles in all prechilling treatments than in no chilling but C. pilosula showed the similar result only in the 8 days prechilling.

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Effects of Light and Storage Condition on the Germination of Oenothera lamarckiana Seeds (큰달맞이꽃 종자(種子)의 발아(發芽)에 미치는 광(光) 및 저장조건(貯藏條件)의 영향)

  • Kim, J.S.;Hwang, I.T.;Koo, S.J.;Cho, K.Y.
    • Korean Journal of Weed Science
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    • v.7 no.2
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    • pp.130-138
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    • 1987
  • The effects of light and storage conditions on the germination of Oenothera lamarckiana were investigated. Germination was induced by red light, but inhibited by green, blue or infrared. No germination occurred in the dark. Radicle was more photosensitive than other parts of the seed. The rate of germination was proportional to increase in Light intensity ranging from 10 to 3000 Lux. Photosensitivity occurred 6 hrs after water imbibition of air-dried seeds. Maximum photosensitivity was detected 1-2 days after seeding under the light condition at 300 Lux. Germination was almost completed 3 days after seeding. When seeds stored in the chilling and wetting condition were kept at constant temperature in the dark, they germinated well. Air-dried seeds, however, didn't germinate. Whereas, the seeds which kept at alternating temperature had the tendency that seeds stored at room temperature (25$^{\circ}C$) germinated better than those stored at low temperature (4$^{\circ}C$).

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Effect of survival rate of chilled cucumber (Cucumis sativus L.) seedlings as influenced by light exposure and chilling started at different time of day. (냉온처리 전후의 광환경 조건이 오이 묘의 생존율에 미치는 영향)

  • Nam, Yooun-Il;Woo, Young-Hoe;Chum, Hee;Kim, Hyung-Jun
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 2001.11a
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    • pp.156-162
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    • 2001
  • 최근 겨울동안에 폭설과 강풍과 같은 기상이변이 잦아지면서 기상재해와 함께 정전에 의한 시설 원예작물의 저온피해도 빈번하게 발생하고 있으나, 실제적으로는 어느 정도의 피해가 있는지 파악되지 못하고 있으며 피해 예측에 관한 정보도 축적되어 있지 못한 실정으로 이에 관련된 연구가 요구되고있다. 시설 재배시 겨울철 온도환경 관리에 대한 연구는 주로 난방비 절약을 위한 저온관리 한계온도 구명(Tanaka 등, 1986), 또는 근권부 온도 조절에 의한 작물의 생산성 증진 연구와 같은 실용적 측면의 연구(Lee, 1994; Fujie와 Saidou, 1983) 및 저온 장해의 생리기작(Herner, 1990; Patterson 등, 1976; Reyes와 Jennings, 1994; Wolk와 Herner, 1982; Wright and Simon, 1973)에 관련된 연구가 수행되고 있으며 냉온 피해를 받을 당시의 전후환경조건과 산화작용에 관련된 연구는 토마토(Kerdnaimongkol 등, 1997) 등 몇 개 작물(King 등 1982)에서 보고된바 있다.(중략)

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The effect of dilution solvent ratio on dewaxing of waxy oils (Waxy Oil 탈납에 있어서 용제희석의 영향)

  • 김주항
    • Tribology and Lubricants
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    • v.3 no.2
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    • pp.34-43
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    • 1987
  • The effect of dilution solvent ratio, and the mixing ratio of MEK and toluene on the solvent dewaxing process has been studied. The results of this study are Summarized as follows; 1. The best mixing ratio of solvent of MEK process and Toluene when in case of light and medium Waxy oils, which has low viscosity was 48: 52, and when in case of heavy waxy oil, which has high viscosity was 45: 55. 2. The best dilution solvent ratio when in case of low viscosity oils. was 1:2.8 against waxy oils after annexing 5 times divided as well as, when incase of high viscosity oil was 1:3.5 after annexing 2 times divided. 3. The chilling temperature was -26$\circ$C and the reguired dewaxing time depends on the viscosity of waxy oils.