• 제목/요약/키워드: life stages

검색결과 1,611건 처리시간 0.047초

패션상표의 수명주기에 따른 마케팅 활동, 상표자산, 소비행동 간의 관계 (Relationship among Marketing Activities, Brand Equity, and Consumer Behavior by Life Cycle of Fashion Brand)

  • 정나영;김미영
    • 한국의류학회지
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    • 제34권9호
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    • pp.1454-1469
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    • 2010
  • Brands have their own life cycles and exert a great influence on the marketing activities of companies. This study examines the marketing activities according to a brand life cycle and measures the scope of their performances. The research divides a fashion brand life cycle into three stages through the analysis of secondary data, and validates the causal relationship between marketing activities, brand equity, and consumer behavior according to the brand life cycle. A total of 573 responses were analyzed through a factor analysis, path analysis, and paired t-test with SPSS 12.0. The results are as follows: According to the analysis of the relationship between marketing mix and brand equity, distribution strategies are effective at the introduction/growth stage and the continuation stage. Advertisement strategies should be a main focus at the maturity stage for brand awareness. Throughout all the stages, product strategies wield the greatest influence on the brand image. Among brand equity components, the brand image has an influence on consumer behavior at every stage of the cycle while the brand awareness has no significant effect on consumer behavior. The marketing mix component that has the greatest impact on consumer behavior is product. Contrary to general expectations, price has a negative or insignificant effect on consumer behavior at every stage of the cycle. The results illustrated in this study help to understand the life cycle of fashion brands and characteristics different from consumer goods. Thus, fashion companies should identify at which stage their brands are positioned and develop different strategies to fit each stage.

Plant RNA Virus Sequences Identified in Kimchi by Microbial Metatranscriptome Analysis

  • Kim, Dong Seon;Jung, Ji Young;Wang, Yao;Oh, Hye Ji;Choi, Dongjin;Jeon, Che Ok;Hahn, Yoonsoo
    • Journal of Microbiology and Biotechnology
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    • 제24권7호
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    • pp.979-986
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    • 2014
  • Plant pathogenic RNA viruses are present in a variety of plant-based foods. When ingested by humans, these viruses can survive the passage through the digestive tract, and are frequently detected in human feces. Kimchi is a traditional fermented Korean food made from cabbage or vegetables, with a variety of other plant-based ingredients, including ground red pepper and garlic paste. We analyzed microbial metatranscriptome data from kimchi at five fermentation stages to identify plant RNA virus-derived sequences. We successfully identified a substantial amount of plant RNA virus sequences, especially during the early stages of fermentation: 23.47% and 16.45% of total clean reads on days 7 and 13, respectively. The most abundant plant RNA virus sequences were from pepper mild mottle virus, a major pathogen of red peppers; this constituted 95% of the total RNA virus sequences identified throughout the fermentation period. We observed distinct sequencing read-depth distributions for plant RNA virus genomes, possibly implying intrinsic and/or technical biases during the metatranscriptome generation procedure. We also identified RNA virus sequences in publicly available microbial metatranscriptome data sets. We propose that metatranscriptome data may serve as a valuable resource for RNA virus detection, and a systematic screening of the ingredients may help prevent the use of virus-infected low-quality materials for food production.

Keeping house: evaluation of housekeeping genes for real-time PCR in the red alga, Bostrychia moritziana (Florideophyceae)

  • Shim, Junbo;Shim, Eunyoung;Kim, Gwang Hoon;Han, Jong Won;Zuccarello, Giuseppe C.
    • ALGAE
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    • 제31권2호
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    • pp.167-174
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    • 2016
  • Biological response of cells to variable conditions should affect the expression level of certain genes. Quantification of these changes in target genes needs stable internal controls. Real-time quantitative polymerase chain reaction (PCR) has traditionally used reference or ‘housekeeping’ genes, that are considered to maintain equal expression in different conditions, to evaluate changes in target genes between samples and experimental conditions. Recent studies showed that some housekeeping genes may vary considerably in certain biological samples. This has not been evaluated in red algae. In order to identify the optimal internal controls for real-time PCR, we studied the expression of eleven commonly used housekeeping genes; elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase, β-actin, polyubiquitin, 30S ribosomal gene, 60S ribosomal gene, beta-tubulin, alpha-tubulin, translation initiation factor, ubiquitin-conjugating enzyme, and isocitrate dehydrogenase in different life-history stages of Bostrychia moritziana. Our results suggest that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 30S ribosomal gene, have the most stable gene expression levels between the different life history stages (male, female, carposporophyte, and tetrasporophyte), while the other genes are not satisfactory as internal controls. These results suggest that the combinations of GAPDH and 30S would be useful as internal controls to assess expression level changes in genes that may control different physiological processes in this organism or that may change in different life history stages. These results may also be useful in other red algal systems.

외국인 유학생의 정착단계에 따른 정보추구행위 (Information Seeking Behaviors throughout the Settlement Stages among International Students in Korea)

  • 윤정원
    • 한국문헌정보학회지
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    • 제56권1호
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    • pp.27-45
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    • 2022
  • 한국에 거주하는 외국인 유학생들의 증가에도 불구하고, 이들의 일상생활에서의 정보추구행위에 관한 연구는 미진한 상황이다. 본 연구는 한국에 거주하는 외국인 유학생들의 정착단계(입국 전, 초기정착단계, 현재)에 따른 정보요구 및 정보탐색행위를 결정적사건기법(Critical Incident Technology: CIT)을 통하여 조사하였다. 한국에 거주하는 외국인 유학생들은 일상생활과 관련된 정보요구를 학교생활 또는 학업에 관련된 정보요구보다 빈번하게 보고하였으며, 과제 등 학습과 직결된 정보요구는 현재 단계에 이르러서야 보고되었다. 유학생들은 정보요구를 해결하기 위해 웹사이트, 소셜미디어, 친구 등 여러 정보원을 사용하였다. 일방향 정보원인 웹사이트에 대하여는 부정적인 경험을 토로하는 경우가 많은 반면, 소셜미디어나 친구 등의 양방향 정보원에 대한 만족도는 높았다. 국내 유학생들이 정보탐색과정에서 경험하는 가장 큰 어려움은 언어 문제인 것으로 나타났으며, 그 외에 한국의 사회시스템에 익숙하지 못해 겪는 어려움도 보고되었다. 정착단계별로 변화하는 유학생들의 정보요구와 정보탐색행위에 대한 이해를 바탕으로, 외국인 유학생들의 일상생활에서의 정보탐색행위를 향상시킬 수 있는 방안이 논의되었다.

랫드에서 고환독성의 정색을 위한 정량적 평가법의 확립: 2-bromopropane의 예 (Establishment of Quantitative Evaluation Method for Screening Testicular Toxicity in Rats: 2-Bromopropane as an Example)

  • 차신우;배주현;손우찬;신진영;신동호;김성호;박승춘;김종춘
    • 생명과학회지
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    • 제15권3호
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    • pp.387-396
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    • 2005
  • The aims of the study were to establish a short-term screening test for detecting testicular toxicity of chemicals in rats and to determine whether a 2-week administration period is sufficient to detect testicular toxicity of 2-bromopropane (2-BP) as an example. Male Sprague-Dawley rats were subcutaneously administered with 1000 mg/kg/day of 2-BP or its vehicle for 2 weeks. Ten male rats each were sacrificed on days 3, 7 and 14 after the initiation of treatment. Parameters of testicular toxicity included genital organ weights, testicular sperm head counts, epididymal sperm counts, motility and morphology, and qualitative and quantitative histopathologic examinations. The early histopathological changes observed on day 3 of treatment included degeneration of spermatogonia and spermatocytes, multinuclear giant cells, mature spermatid retention, vacuolization of Sertoli cells, and decreased number of spermatogonia in stages II and V. On day 7 of treatment, atrophy of seminiferous tubules, exfoliation of germ cells, degeneration of spermatogonia and spermatocytes, multinuclear giant cells, mature spermatid retention, vacuolization of Sertoli cells, decreased number of spermatogonia in stages II and V, and decreased number of spermatocytes in stages VII and XII. On day 14 after treatment, a significant decrease in the weights of testes and seminal vesicles was found. Atrophy of seminiferous tubules, exfoliation of germ cells, degeneration of spermatogonia and spermatocytes, mature spermatid retention, vacuolization of Sertoli cells, decreased number of spermatogonia in stages II and V, and decreased number of spermatocytes in all spermatogenic stages were also observed. In addition, a slight non-significant decrease in testicular sperm head counts, daily sperm production rate and epididymal sperm counts was found. The results showed that 2 weeks of treatment is sufficient to detect the adverse effects of 2-BP on male reproductive organs. It is considered that the short-term testicular toxicity study established in this study can be a useful tool for screening the testicular toxic potential of new drug candidates in rats.

Formation of Sensory Pigment Cells Requires Fibroblast Growth Factor Signaling during Ascidian Embryonic Development

  • Kim, Gil-Jung
    • Animal cells and systems
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    • 제7권3호
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    • pp.221-225
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    • 2003
  • The tadpole larva of the ascidian Halocynthia roretzi has two sensory pigment cells in its brain vesicle. To elucidate the temporal requirement for FGF signaling in formation of the pigment cells, embryos were treated with an FGF receptor 1 inhibitor, SU5402, or an MEK inhibitor, U0126 during various embryonic stages. In the present study, it is shown that the embryos treated with SU5402 from the 16-cell stage to the early gastrula stage do not form pigment cells, whereas those treated after the early gastrula stage form pigment cells. In pigment cell formation, embryos suddenly exhibited the sensitivity to SU5402 only for 1 h at the neural plate stage(-4 h after the beginning of gastrulation). When U0126 treatment was carried out at various stages between the 8-cell and late neurula stages, the embryos scarcely formed pigment cells. Pigment cell formation occurred when the embryos were placed in U0126 at early tail bud stage. These results indicate that FGF signaling is involved in pigment cell formation at two separate processes during ascidian embryogenesis, whereas more prolonged period is required for MEK signaling.