• The Plant Pathology Journal
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• v.31 no.2
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• pp.108-114
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• 2015

Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China, and several key pathogenic factors have been isolated. An yeast two-hybrid (Y2H) library is a very useful platform to further unravel novel pathogenic factors in C. lunata. To construct a high-quality full length-expression cDNA library from the C. lunata for application to pathogenesis-related protein-protein interaction screening, total RNA was extracted. The SMART (Switching Mechanism At 5' end of the RNA Transcript) technique was used for cDNA synthesis. Double-stranded cDNA was ligated into the pGADT7-Rec vector with Herring Testes Carrier DNA using homologous recombination method. The ligation mixture was transformed into competent yeast AH109 cells to construct the primary cDNA library. Eventually, a high qualitative library was successfully established according to an evaluation on quality. The transformation efficiency was about $6.39{\times}10^5$ transformants/$3{\mu}g$ pGADT7-Rec. The titer of the primary cDNA library was $2.5{\times}10^8cfu/mL$. The numbers for the cDNA library was $2.46{\times}10^5$. Randomly picked clones show that the recombination rate was 88.24%. Gel electrophoresis results indicated that the fragments ranged from 0.4 kb to 3.0 kb. Melanin synthesis protein Brn1 (1,3,8-hydroxynaphthalene reductase) was used as a "bait" to test the sufficiency of the Y2H library. As a result, a cDNA clone encoding VelB protein that was known to be involved in the regulation of diverse cellular processes, including control of secondary metabolism containing melanin and toxin production in many filamentous fungi was identified. Further study on the exact role of the VelB gene is underway.

• Korean Journal of Microbiology
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• v.40 no.4
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• pp.359-363
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• 2004

I investigated an effective way to generate a metagenomic library from DNA prepared from environental samples. The sizes of DNA extracted from environmental samples were usually in the range of 10 to 100 kbp as estimated from $0.4\%$ agarose gel electrophoresis. Because of this small size, a fosmid, rather than BAC, was chosen as a vector. It was found that, for the successful generation of metagenomic library, the selection of DNA with the sized of about 40 kbp was critical and, therefore, a simple agarose gel electrophoresis system was developed to select this size of DNA. By the procedure described in this report, I obtained metagenomic libraries containing 25,000 fosmid clones, which corresponded to 1,000 Mb of metagenomic DNA.

• Journal of the Korean Society for Library and Information Science
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• v.36 no.4
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• pp.123-145
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• 2002

This study Is to investigate extensively the complaint responses of the academic library users, with a special regard to those in Daegu Metropolitan City and in Kyungpook Province. It selects and analyzes empirically some of the antecedents of the complaining behaviors including perceived severity of dissatisfaction, perception of free un, attitude toward complaining, cost of the complaining, likelihood of success, product/service importance, external attributions and loyalty : and complaint responses including exit, voice(redress seeking), negative word-of-mouth and third party complaints, of the academic library users, based on the theoretical backgrounds. Final data through questionaries are obtained from 582 unsatisfied academic library users in 5 universities in those areas, 542 of them analysed with SPSS 11.0 for Windows.

• Journal of Korean Society for Quality Management
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• v.46 no.4
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• pp.1015-1028
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• 2018

Purpose: This study attempts to find how the cultural marketing activities utilizing cultural space affect firm's brand image and brand loyalty, and whether this culture marketing technique is recognized as a social contribution activity by potential customers and thereby influence brand image and loyalty. Methods: The data was collected by using the structured questionnaires to consumers who have experience using the Naver Library, Starfield Library, and Hyundai Card Library. The proposed research model is tested using 178 valid questionnaires using Smart PLS 2.0. Results: This research indicated that among cultural marketing factors, cultural support and cultural promotion have little impact on brand image, whereas culture firm influence brand image. Brand image also had an impact on brand loyalty, but it was found that public libraries operated by companies were not recognized as corporate social contribution activities, nor did they have an impact on the formation of the company's brand image. Conclusions: The study offered a theoretical and empirical foundation for future research by empirically identifying the relationship between cultural marketing and brand image and loyalty and confirmed the coordinating effect of social contribution activities between a cultural firm and its brand image.

• Journal of the Korean Society for Library and Information Science
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• v.48 no.4
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• pp.345-376
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• 2014

This study examined (1) what effect emotional labor has on an university library, focusing on (2) the relationship among a supervisor's emotional intelligence, the extent of social support and the level of library service on job performance. The survey period took place from 14 Oct. to 4 Nov. 2013. 533 librarians at 13 public and 28 private university libraries were included in the survey. Of the 533 surveys distributed, 529 were returned and used in the final analysis. SPSS Win 21.0 was used for statistical analysis, factor analysis, regression analysis and differential analysis. The survey also shows that a librarian's emotional labor affects emotional intelligence of supervisor, social support and library service level positively. This finding is not the case for the employees in the general service industry. Because the librarian is professional and manages stress better than general employees. This research suggest the following practical measures. Educational programs for librarian's emotional intelligence should be planned in order to improve library service.

• Journal of Microbiology
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• v.45 no.1
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• pp.1-5
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• 2007

The bacterial compositions between the dental unit water system and human saliva were characterized and compared by direct sequence analysis of 16S rDNA clone libraries. Based on the species richness estimation, bacterial diversity in the dental unit water system (DUW) was more diverse than that of the human saliva (HS). The Chaol estimates of species richness in HS and DUW samples were 12.0 and 72.4, respectively. The total numbers of OTUs observed in the combined libraries accounted for 83% (HS) and 59% (DUW) of the Chaol diversity estimate as defined at the 80% similarity threshold. Based on the sequence analysis, the phylum Proteobacteria was the major group in both clone libraries at phylum level. DUW clone library contained 80.0% Proteobacteria, 8.0% Bacteroides, 4.0% Nitrospira, 4.0% Firmicutes, 2.0% Planctomycetes and 2.0% Acidobacteria. On the other hand, human saliva (HS) clone library contained 55.5% Proteobacteria, 36.1% Firmicutes and 8.4% Bacteroides. The majority of bacteria identified belonged to phylum Proteobacteria in both samples. In dental unit water system (DUW), Alphaproteobacteria was detected as the major group. There was no evidence of the bacterial contamination due to a dental treatment. Most sequences were related to microorganisms derived from biofilm in oligotrophic environments.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.23 no.2
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• pp.151-169
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• 2012

This research analyzed and compared the public library satisfaction level between South Korea and USA public library user. This research looked at the public library user's nominal complains to the library from various countries and analyzed the impact variance of customer satisfaction level by using four key factors(faculty, facility, online service, books). The results were, first, overall customer satisfaction level of USA average(m=3.91) was higher then South Korea average(m=3.08) with a difference of m=0.83. Second, in the outcome of the survey conducted in South Korea, all four key factors were strongly related to customer satisfaction level, whereas the survey conducted in the USA, only two key factors showed relation to the customer satisfaction level which were facility and online service. Lastly, In the survey conducted in South Korea, out of the four key factors, Faculty(${\beta}$=.838) and online service(${\beta}$=.496) were the only two key factors that influenced the customer satisfaction level. In the survey conducted in the USA, Facility(${\beta}$=.441) and online service(${\beta}$=.471) were the two key factors that influenced customer satisfaction level. Therefore, South Korea should invest in increasing online service and educating faculty service as well as managing more intensively in software type field policies.

• Nuclear Engineering and Technology
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• v.49 no.8
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• pp.1610-1616
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• 2017

To validate the new Evaluated Nuclear Data File $(ENDF)/B-VIII.0{\beta}4$ library, 31 different critical cores were selected and used for a benchmark test of the important parameter keff. The four utilized libraries are processed using Nuclear Data Processing Code (NJOY2016). The results obtained with the $ENDF/B-VIII.0{\beta}4$ library were compared against those calculated with ENDF/B-VI.8, ENDF/B-VII.0, and ENDF/B-VII.1 libraries using the Monte Carlo N-Particle (MCNP(X)) code. All the MCNP(X) calculations of keff values with these four libraries were compared with the experimentally measured results, which are available in the International Critically Safety Benchmark Evaluation Project. The obtained results are discussed and analyzed in this paper.

• Journal of Korean Library and Information Science Society
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• v.15
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• pp.113-151
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• 1988

The enumerative classification schemes do not represent the tiny mass of knowledge embodied in a article in a periodical or in a chapter or a paragraph of a book. But today's information centers regard a tiny spot of knowledge embodied in a article as a class. we call this micro-thought. But the enumerative classification are manual systems, it cannot be a n.0, pplied to the automation of classification. The purpose of this study is to build a general principle for the automatic book-classification which can be put to use in library operation, and to present a methodology of the automatic classification for the library. The methodology used is essentially theoretical, Published works by and about Ranganathan, especially 6th edition of the CC were studied, analyzed. The principle of automatic book classification derived from the analysis of colon classification and facet combinations. The results of this study can be summarized as follows ; (1) This study confined the fields of library science and agriculture. (2) This study represent a general principles for the automatic book classification of library science and agriculture. (3) Program flowcharts are designed as a basis of system analysis and program procedure in library science and agriculture. (4) The principles of the automatic classification in library, science is different from that of agriculture. (5) Automatic book classification can be performed by the principle of faceted classification, by inputting the title and subject code into a computer. In addition, the expected value from the automatic book-classification is as follows (1) The prompt and accurate of classification is possible. (2) Though a book is classified in any library, it can have same classification number. (3) The user can retrieve the classification code of a book for which he or she wants to search through dialogue with the computer. (4) Since the concept coordination method is employed, a tiny mass of knowledge embodied in a article in a periodical or in a chapter or a paragraph of a book can be represented as a class. (5) By performing automatic book-classification, the automation of library operation can be completed.

• Journal of Korean Library and Information Science Society
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• v.7
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• pp.177-206
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• 1980

Public library legislation is the essential means of providing for all the citizen in modern society with a standard public library service. We can trace the origin of that back to the Public Libraries Act 1850 in England. This paper is reviewing all the measures concerning public libraries of England since then in order to find out some guidelines for revision of the Korean Library Law enacted in 1963. Although Public Libraries Act 1850 was an output of a series of social reform movement in the 19th century, it established first the provision of public library service su n.0, pported by public funds. However the Act had several restrictions hindering public library service from making progress. These are: 1. Adoptive legislation in nature 2. Limitation of the size of population and rate income 3. Small library authorities 4. Procedure for adoption by public meeting 5. Restrictions on the ways in which rate income might be spent 6. No governmental organization to direct or control for a standardized public library service 7. No cooperative schemes among libraries. Subsequent development was directed largely to removal of those limitations. The present Public Libraries Act 1964 as a model of public library legislation was enacted on the basis of such reports as Kenyon, McColvin, Roberts, and Bourdillon. All but the problem of small library authorities were removed with this new Act. The final object could be achieved by the Local Government Act 1974 which reorganized existing 383 small authorities into new 115 ones the average population of which was 280, 000. Now, McColvin's dream of 30 years could be fulfilled in the late 70's. He argued that the minimum size of population of a viable authority should be 300, 000. Although the effect of the 1964 Act on the development of public library services in England was great, there must be some considerations as to the provisions of public library objective, government funding for library services, free use of library materials to all regardless of residence, and placement of library committee within the authority.