• Journal of Microbiology and Biotechnology
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• 제21권7호
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• pp.667-674
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• 2011

The gene for esterase (rEst1) was isolated from a new species of genus Rheinheimera by functional screening of E. coli cells transformed with the pSMART/HaeIII genomic library. E. coli cells harboring the esterase gene insert could grow and produce clear halo zones on tributyrin agar. The rEst1 ORF consisted of 1,029 bp, corresponding to 342 amino acid residues with a molecular mass of 37 kDa. The signal P program 3.0 revealed the presence of a signal peptide of 25 amino acids. Esterase activity, however, was associated with a homotrimeric form of molecular mass 95 kDa and not with the monomeric form. The deduced amino acid sequence showed only 54% sequence identity with the closest lipase from Cellvibrio japonicus strain Ueda 107. Conserved domain search and multiple sequence alignment revealed the presence of an esterase/ lipase conserved domain consisting of a GXSXG motif, HGGG motif (oxyanion hole) and HGF motif, typical of the class IV hormone sensitive lipase family. On the basis of the sequence comparison with known esterases/ lipases, REst1 represents a new esterase belonging to the class IV family. The purified enzyme worked optimally at $50^{\circ}C$ and pH 8, utilized pNP esters of short chain lengths, and showed best catalytic activity with p-nitrophenyl butyrate ($C_4$), indicating that it was an esterase. The enzyme was completely inhibited by PMSF and DEPC and showed moderate organotolerance.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2019년도 학술발표회
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• pp.121-121
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• 2019

특정 지역에 집중적으로 비가 내리는 현상인 국지성호우가 빈번히 발생함에 따라 하천 주변 사회기반시설의 침수 위험성이 증가하고 있다. 침수 위험성 판단 여부는 주로 수위정보를 이용하며 수위 예측은 대부분 수치모형을 이용한다. 본 연구에서는 빅데이터 기반의 RNN(Recurrent Neural Networks)기법 알고리즘을 활용하여 수위를 예측하였다. 연구대상지는 조위의 영향을 많이 받는 한강 전역을 대상으로 하였다. 2008년~2018년(10개년)의 실제 침수 피해 실적을 조사한 결과 잠수교, 한강대교, 청담대교 등에서 침수 피해 발생률이 높게 나타났고 SNS(Social Network Services)와 같은 비정형화 자료에서는 청담대교가 가장 많이 태그(Tag)되어 청담대교를 연구범위로 설정하였다. 본 연구에서는 Python에서 제공하는 Tensor flow Library를 이용하여 수위예측 알고리즘을 적용하였다. 데이터는 정형화 데이터와 비정형 데이터를 사용하였으며 정형화 데이터는 한강홍수 통제소나 기상청에서 제공하는 최근 10년간의 (2008~2018) 수위 및 강우량 자료를 수집하였다. 비정형화 데이터는 SNS를 이용하여 민간 정보를 수집하여 정형화된 자료와 함께 전체자료를 구축하였다. 민감도 분석을 통하여 모델의 은닉층(5), 학습률(0.02) 및 반복횟수(100)의 최적값을 설정하였고, 24시간 동안의 데이터를 이용하여 3시간 후의 수위를 예측하였다. 2008년~ 2017년 까지의 데이터는 학습 데이터로 사용하였으며 2018년의 수위를 예측 및 평가하였다. 2018년의 관측수위 자료와 비교한 결과 90% 이상의 데이터가 10% 이내의 오차를 나타내었으며, 첨두수위도 비교적 정확하게 예측되는 것을 확인하였다. 향후 수위와 강우량뿐만 아니라 다양한 인자들도 고려한다면 보다 신속하고 정확한 예측 정보를 얻을 수 있을 것으로 기대된다.

• Animal Bioscience
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• 제35권7호
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• pp.975-988
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• 2022

Objective: In this study, we aimed to identify long non-coding RNAs (lncRNAs) that play important roles in starvation stress, analyze their functions, and discover potential molecular targets to alleviate starvation stress to provide a theoretical reference for subsequent in-depth research. Methods: We generated a piglet starvation stress animal model. Nine Yorkshire weaned piglets were randomly divided into a long-term starvation stress group (starved for 72 h), short-term starvation stress group (starved for 48 h), and the control group. LncRNA libraries were constructed using high-throughput sequencing of piglet ileums. Results: We obtained 11,792 lncRNAs, among which, 2,500 lncRNAs were novel. In total, 509 differentially expressed (DE)lncRNAs were identified in this study. Target genes of DElncRNAs were predicted via cis and trans interactions, and functional and pathway analyses were performed. Gene ontology functions and Kyoto encyclopedia of genes and genomes analysis revealed that lncRNA-targeted genes mainly participated in metabolic pathways, cellular processes, immune system processes, digestive systems, and transport activities. To reveal the mechanism underlying starvation stress, the interaction network between lncRNAs and their targets was constructed based on 26 DElncRNAs and 72 DEmRNAs. We performed an interaction network analysis of 121 DElncRNA-DEmRNA pairs with a Pearson correlation coefficient greater than 0.99. Conclusion: We found that MSTRG.19894.13, MSTRG.16726.3, and MSTRG.12176.1 might play important roles in starvation stress. This study not only generated a library of enriched lncRNAs in piglets, but its outcomes also provide a strong foundation to screen key lncRNAs involved in starvation stress and a reference for subsequent in-depth research.

• 대한치과교정학회지
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• 제52권5호
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• pp.334-344
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• 2022

Objective: This study aimed to systematically analyze the effect and stability of miniscrew-assisted rapid palatal expansion (MARPE) to provide a reference for the clinical treatment of patients with maxillary transverse deficiency (MTD). Methods: We searched PubMed, Science Direct, Web of Science, Embase, Cochrane Library, CNKI, and Wanfang Database for relevant studies published before February 18, 2021 and selected them according to the eligibility criteria. The Cochrane Handbook for Systematic Reviews (version 5.1.0) criteria were used for the quality assessment of randomized controlled trials, while the scoring protocol of the methodological index for non-randomized studies was used for non-randomized controlled trials. Statistical analysis was performed using the RevMan5.3 software. Results: All the included studies showed a relatively high success rate of expansion. The changes in both the intermolar and alveolar widths after MARPE were statistically significant. MARPE exhibited greater skeletal expansion effects than did conventional RPE. The midpalatal suture was opened in parallel after MARPE. A small amount of relapse was observed 1 year after expansion. MARPE caused tooth inclination and a decrease in alveolar height, but it was less significant than in conventional RPE. Conclusions: MARPE may be an effective treatment modality for patients with MTD. It causes great transverse skeletal expansion in late adolescence. In comparison to conventional RPE, MARPE has lower detrimental periodontal effects and has certain clinical advantages.

• 치위생과학회지
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• 제24권1호
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• pp.9-21
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• 2024

Background: The purpose of this study was to utilize text network analysis and topic modeling to identify interconnected relationships among keywords present in patent information related to oral health, and subsequently extract latent topics and visualize them. By examining key keywords and specific subjects, this study sought to comprehend the technological trends in oral health-related innovations. Furthermore, it aims to serve as foundational material, suggesting directions for technological advancement in dentistry and dental hygiene. Methods: The data utilized in this study consisted of information registered over a 20-year period until July 31st, 2023, obtained from the patent information retrieval service, KIPRIS. A total of 6,865 patent titles related to keywords, such as "dentistry," "teeth," and "oral health," were collected through the searches. The research tools included a custom-designed program coded specifically for the research objectives based on Python 3.10. This program was used for keyword frequency analysis, semantic network analysis, and implementation of Latent Dirichlet Allocation for topic modeling. Results: Upon analyzing the centrality of connections among the top 50 frequently occurring words, "method," "tooth," and "manufacturing" displayed the highest centrality, while "active ingredient" had the lowest. Regarding topic modeling outcomes, the "implant" topic constituted the largest share at 22.0%, while topics concerning "devices and materials for oral health" and "toothbrushes and oral care" exhibited the lowest proportions at 5.5% each. Conclusion: Technologies concerning methods and implants are continually being researched in patents related to oral health, while there is comparatively less technological development in devices and materials for oral health. This study is expected to be a valuable resource for uncovering potential themes from a large volume of patent titles and suggesting research directions.

• Korean Circulation Journal
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• 제54권3호
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• pp.126-137
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• 2024

Background and Objectives: The impact of off-hours admission (such as weekends, nighttime, and non-working hours) vs. regular hours (weekdays and daytime working hours) on the mortality risk of patients undergoing surgery for type A aortic dissection (TAAD) repair is still uncertain. To address this uncertainty, we undertook a comprehensive systematic review and meta-analysis. We aimed to assess the potential link between off-hours admission and the risk of mortality in patients undergoing TAAD repair surgery. Methods: We conducted a thorough search of the PubMed, Embase, and Cochrane Library databases, covering the period from their inception to May 20, 2023. Our inclusion criteria encompassed all studies that examined the potential relationship between off-hour admission and mortality in individuals who had undergone surgery for TAAD repair. The odds ratios (ORs) were extracted and combined utilizing a random effects model for our synthesis. Results: Nine studies with 16,501 patients undergoing TAAD repair surgery were included in the meta-analysis. Overall, patients who underwent surgery during the weekend had higher in-hospital mortality (pooled OR, 1.41; 95% confidence interval [CI], 1.14-1.75; p=0.002) than those treated on weekdays. However, the mortality risks among patients who underwent TAAD surgery during nighttime and non-working hours were not significantly elevated compared to daytime and working hours admission. Conclusions: Weekend surgery for TAAD was associated with a higher in-hospital mortality risk than weekday surgery. However, further studies are warranted to identify and develop strategies to improve the quality of round-the-clock care for patients with TAAD.

• Animal Bioscience
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• 제36권1호
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• pp.10-18
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• 2023

Objective: In this study, we aimed to position the Hungarian Merino among other Merinoderived sheep breeds, explore the characteristics of our sampled animals' genetic similarity network within the breed, and highlight single nucleotide polymorphisms (SNPs) associated with daily weight-gain. Methods: Hungarian Merino (n = 138) was genotyped on Ovine SNP50 Bead Chip (Illumina, San Diego, CA, USA) and positioned among 30 Merino and Merino-derived breeds (n = 555). Population characteristics were obtained via PLINK, SVS, Admixture, and Treemix software, within-breed network was analysed with python networkx 2.3 library. Daily weight gain of Hungarian Merino was standardised to 60 days and was collected from the database of the Association of Hungarian Sheep and Goat Breeders. For the identification of loci associated with daily weight gain, a multi-locus mixed-model was used. Results: Supporting the breed's written history, the closest breeds to Hungarian Merino were Estremadura and Rambouillet (pairwise F_ST values are 0.035 and 0.036, respectively). Among Hungarian Merino, a highly centralised connectedness has been revealed by network analysis of pairwise values of identity-by-state, where the animal in the central node had a betweenness centrality value equal to 0.936. Probing of daily weight gain against the SNP data of Hungarian Merinos revealed five associated loci. Two of them, OAR8_17854216.1 and s42441.1 on chromosome 8 and 9 (-log₁₀P>22, false discovery rate<5.5e-20) and one locus on chromosome 20, s28948.1 (-log₁₀P = 13.46, false discovery rate = 4.1e-11), were close to the markers reported in other breeds concerning daily weight gain, six-month weight, and post-weaning gain. Conclusion: The position of Hungarian Merino among other Merino breeds has been determined. We have described the similarity network of the individuals to be applied in breeding practices and highlighted several markers useful for elevating the daily weight gain of Hungarian Merino.

• 한국정보통신학회논문지
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• 제20권7호
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• pp.1296-1302
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• 2016

경량 암호기술 표준인 ISO/IEC 29192-2에서 블록암호 표준으로 지정된 초경량 블록암호 알고리듬 PRESENT의 하드웨어 구현에 대해 기술한다. 암호 전용 코어와 암호/복호 기능을 갖는 두 종류의 PR80 크립토 코어를 80 비트의 마스터키를 지원하도록 설계하였다. 설계된 PR80 크립토 코어는 블록암호의 기본 ECB (electronic code book) 운영모드를 수행하며, 마스터키 재입력 없이 평문/암호문 블록들을 연속적으로 처리할 수 있도록 설계되었다. PR80 크립토 코어는 Verilog HDL을 사용하여 소프트 IP로 설계되었으며, Virtex5 FPGA에 구현하여 정상 동작함을 확인하였다. 설계된 코어를 $0.18{\mu}m$ 공정의 CMOS 셀 라이브러리로 합성한 결과, 암호 전용 코어와 암호/복호 코어는 각각 2,990 GE와 3,687 GE로 구현되어 적은 게이트를 필요로 하는 IoT 보안 응용분야에 적합하다. 암호 전용 코어와 암호/복호 코어의 최대 동작 주파수는 각각 500 MHz와 444 MHz로 평가되었다.

• 전자공학회논문지
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• 제50권6호
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• pp.145-155
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• 2013

본 연구에서는 8K UHD($7680{\times}4320$) 영상을 실시간 부호화하기 위한 $4{\times}4$ 블록 부화소 움직임추정기를 제안한다. 연산처리성능을 향상시키기 위해 보간 연산을 $4{\times}4$ 블록 단위로 병렬화시켰으며, 병렬 보간 연산에서 필요한 메모리 대역폭을 확장하기 위해 $10{\times}10$개의 메모리 어레이를 가진 2D 캐쉬 버퍼 구조를 설계하였다. 그리고 2D 캐쉬 버퍼는 검색영역 간 재사용 기법을 적용하여 참조화소의 중복저장을 최소화하였으며, $4{\times}4$ 블록 병렬 보간 필터는 3단(수평 수직 1/2부화소, 대각선 1/2부화소, 1/4부화소) 평면 보간 연산 파이프라인 구조로 설계하여 연산회로를 고속화시켰다. 0.13um 공정에서 시뮬레이션한 결과, 436.5K게이트의 $4{\times}4$ 블록 부화소 움직임추정기는 동작주파수 187MHz에서 8K UHD급 동영상을 초당 30프레임으로 실시간 처리하는 성능을 보였다.

• 한국미생물·생명공학회지
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• 제17권6호
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• pp.600-605
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• 1989

Escherichia coli 내에서 프로모터활성을 보이는 Zymomonas mobilis 유래의 유전자 절편을 분리하고 특성을 분석하였다. 프로모터 탐색용 벡터인 pCMT215는 promoter activity가 없는 pMT21의 HinIII 위치에 pYEJ001의 클로람페니콜 아세틸전이 효소유전자를 함유한 0.7-kb HindIII 조각을 접합시켜 제조하였다. E. mobilis의 chromosomal DNA를 Sau3AI으로 부분절단하여 pCMT215에 도입한 후, 이를 이용하여 대장균을 형질전환시킨 결과 14개의 형질전환주가 선별되었다. 이들은 30-750 $\mu$g/$m\ell$ 농도의 chloramphenicol에 내성을 보였으며 클로닝된 유전자조각의 크기는 0.1-1.5Kb였다. 이 가운데 5개의 염기서열을 분석해 본 결과 일반적인 프로모터의 염기서열과 많은 유사점이 발견되었는데, 대장균의 프로모터인 -35 또는 -10 지역과의 부분적인 일치와 A 또는 T 염기가 풍부한 지역과 연속적인 A 또는 T 염기배열, 그리고 회문형태의 염기서열 등이 발견되었다. 또한 대장균 내에서의 프라이머 연장실험결과 Z. mobilis로부터 유래된 DNA조각에서 전사의 시작이 4-170 염기의 거리를 두고 두 곳 또는 여러 곳에서 일어남을 알 수 있었다.