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Studies on the Development of Acid Tolerant and Superior Nitrogen Fixation Symbionts for Pasture on Hilly Land -II. Selection of Acid Tolerant R. meliloti in virto and Inoculation Effect in Soils (야산(野山) 목초지용(牧草地用) 내산성(耐酸性) 우수(優秀) 질소고정균주(窒素固定菌株) 개발(開發) -II. 내산성(耐酸性) R. meliloti 의 기내선발(器內選拔) 및 토양(土壤) 접종효과(接種效果))

  • Kang, Ui-Gum;Choi, Ju-Hyeon;Cho, Kang-Jin;Jung, Yeun-Tae;Cho, Moo-Je
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.1
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    • pp.72-77
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    • 1989
  • A study was conducted to obtain acid tolerant and superior Rhizobium meliloti strain for alfalfa on hilly acid soils. With host plant, eight isolates of R, meliloti selected in the vicinity of Milyang were evaluated for their ability to establish symbiotic effectiveness in acidified tube culture medium and vermiculite pot with different urea levels. Among isolates "YA03" was characterized for the ability to manifest to acid tolerance in three different soils of which pH were 5.0, 6.0, and 7.5. The results obtained were summarized as follows: 1. Of eight isolates "YA03" and "YA09" performed nodule in the tube medium of pH5.0, and in the symbiotic effectiveness YA03 was superior to others. 2. Alfalfa growth and $N_2-fixing$ activity by the inoculation of "YA03" isolate were better than others at the level of urea 1.25mM as well as nonurea. 3. Application of urea with inoculation of "YA03" islate to alfalfa was one of the effective factors for symbiotic effectiveness. 4. In infertile soil of pH5.0 inoculation of R. meliloti "YA03" to alfalfa caused the increase of shoot dry matter of 320% as compared to the control, and the total amount of yield was 131% as much yield in moderate fertile soil of pH7.5. Finally R. meliloti "YA03" isolate was selected as an acid tolerant strain.

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Study on the Salt Tolerance of Rice and Other Crops in Reclaimed Soil Areas. -9. On the Rooting and yields in Saline Soil of the Rice Seedlings Reared in Different Conditions (간척지(干拓地)에서 수도(水稻) 및 기타작물(其他作物)의 내염성(耐鹽性)에 관(關)한 연구(硏究) -9. 상이(相異)한 조건(條件)들에서 재배(栽培)된 수도묘(水稻苗)의 염분간척지(鹽分干拓地)에서의 활착(活着)과 수량(收量)에 관(關)하여)

  • Im, Hyong Bin;Lim, Ung Kyu;Hoang, Chong Ser
    • Korean Journal of Soil Science and Fertilizer
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    • v.4 no.1
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    • pp.79-85
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    • 1971
  • The rice variety, Kwanok, was reared in the water, land and salty beds and transplanted to the reclaimed soil area having an average of 0.48% salt concentration 0.67% at the end of April Two levels of $NH_3$-N and urea-N with 6 treatments were used. The effect of each treatment was observed. The plant height of the land bed seedlings at transplanting stage was short but the dry-weight/plant-height ratio was large, and the rooting ability vigorous remarkably after transplantation in the salty area. The total carbohydrate content of the stem part was markedly larger in the land bed seedlings than the others and the C/N ratio was accordingly greater. This tendency was observed through the last rooting stage. In the salty nutrient solution, the roots of salty bed seedlings showed high respiratory activity. The activity of the land bed seedlings did not decreased in the hypertonic solution as much as the water bed seedlings. There was no difference in the effect of fertilization on the rough rice yield between ammonium sulphate and urea. The cultural practices with the land and salty bed seedlings increased the rough rice yields by 33% and 22% respectively, compared with the yields of the water bed Seedlings. The number of panicles, panicle weight and the number of grains per panicle were much greater from the rice plant grown by the land bed seedlings than from the other bed seedlings.

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Fractionation and Pollution Index of Heavy Metals in the Sangdong Tungsten Mine Tailings (광미에 존재하는 중금속의 분획화와 오염도 평가)

  • Yang, Jae-E.;Kim, Hee-Joung;Jun, Sang-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.1
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    • pp.33-41
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    • 2001
  • Enormous volumes of mining wastes from the abandoned and closed mines are disposed without a proper treatment in the upper Okdong River basin at Southeastern part of Kangwon Province. Erosion of these wastes contaminates soil, surface water, and sediments with heavy metals. Objectives of this research were to fractionate heavy metals in the mine tailing stored in the Sangdong Tungsten tailing dams and to assess the potential pollution index of each metal fraction. Tailing samples were collected from tailing dams at different depth and analyzed for physical and chemical properties. pH of tailings ranged from 7.3 to 7.9. Contents of total N and organic matter were in the ranges of 3.2~5.5%, and 1.3~9.1%, respectively. Heavy metals in the tailings were higher in the newly constructed tailing dam than those in the old dam. Total concentrations of metals in the tailings were in the orders of Zn > Cu > Pb > Ni > Cd, exceeded the corrective action level of the Soil Environment Conservation Law and higher than the natural abundance levels reported from uncontaminated soils. Relative distribution of heavy metal fractions was residual > organic > reducible > carbonate > adsorbed, reversing the degree of metal bioavailability. Mobile fractions of metals were relatively small compared to the total concentrations. Distribution of metals in the tailing dam profiles was metal specific. Concentrations of Cu at the surface of tailing dams were higher than those at the bottom. Pollution index (PI) values of each fraction of metals were ranged from 4.27 to 8.51 based on total concentrations. PI values of mobile fractions were lower than those of immobile fractions. Results on metal fractions and PI values of the tailing samples indicate that tailing samples were contaminated with heavy metals and had potential to cause a detrimental effects on soil and water environment in the lower part of the stream. A prompt countermeasure to prevent surface of tailings in the dams from water and wind erosions is urgently needed.

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Optimum Fertilization Based on Soil Testing for Chinese Cabbage Cultivation in Plastic Film Houses (시설재배지 토양 검정에 의한 배추의 적정 시비량)

  • Hong, Soon Dal;Kang, Bo Goo;Kim, Jai Joung
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.1
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    • pp.16-24
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    • 1998
  • To determine the optimum application of fertilizers for the cultivation of Chinese cabbage in plastic film house, twenty soils which contain different salts contents were taken from 4 different area of plastic film house cultivation, Youngdong. Boeun county, Cheongweon county, and Cheongju city. The dry weight and the amount of N. P, and K uptakes of Chinese cabbage in the plot of no fertilization were considered as the factors representing the fertility of the soil. And a difference of dry weight and the amounts of N, P, and K uptakes of plants between the plot of fertilization and no fertilization were considered as the factors representing the total effect of fertilizer and fertilizer N, P, and K effects. respectively. These factors of soil fertility and fertilizer effects were estimated by correlation and regression with soil tests in order to find the critical levels and recommended method for optimum fertilization of Chinese cabbage. Chinese cabbage transplanted in two soils, having the electrical conductivity of 9.3 and 15.2 dS/m, were not able to root due to the salts toxicity. The content of inorganic N, the electrical conductivity, and CEC were founded to have significant correlation with the factors of both the soil fertility and fertilizer effects for the cultivation of Chinese cabbage. To determine the weighting degree for the productivity and the fertilizer effects, the standardized partial regression coefficient was analyzed by regression among the factors of fertility, the fertilizer effects, and the soil tests. The coefficient for inorganic N($NH_4-N$ and $NO_3-N$) was obtained as the absolute value of 756-1871 and this value was extremely higher than those of other soil tests which was 0.07-4.11. These results suggested that the content of inorganic N is the best tests for the estimation of the productivity and the fertilizer effects for the cultivation of Chinese cabbage in plastic film house. The critical level of inorganic N($NH_4-N+NO_3-N$) estimated by Cate-Nelson split method for maximum productivity and zero point of fertilizer effect was 220 mg/kg for all the factors of estimation. These results suggested that no application of fertilizer N. P, and K is required at the critical level of inorganic N of soil. Consequently the optimum application of fertilizer N, P, and K for the cultivation of Chinese cabbage in plastic film house was possible to determine by the critical level of inorganic N of soil. The critical level of electrical conductivity was estimated as 2.8 dS/m by the same method.

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Status of Fertilizer Application and Soil Management for Major Vegetable Crops in Farmers' Fields of Alpine Area (고랭지 주요작물의 시비 및 토양관리 실태)

  • Lee, Jeong-Tae;Lee, Gye-Jun;Zhang, Yong-Seon;Hwang, Seon-Woong;Im, Su-Jeong;Kim, Chang-Bae;Mun, Yeong-Hun
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.6
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    • pp.357-365
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    • 2006
  • The investigations were conducted to find out the situation of fertilizer use and the contents of soil chemical components on summer vegetable crops at 791 farmers' upland fields located in the parts of Gangwon-do, Gyengsangbuk-do and Jeollabuk-do of alpine area. Major vegetable crops were potato, Chinese cabbage, radish, carrot, onion, and cabbage. From the location surroundings cultivated alpine vegetable crops, the orders were Gangwon-do>Gyeongsangbuk-do>Jeollabuk-do part in the sizes of a fie1d area and the height above sea level, and Jeollabuk-do>Gyeongsangbuk-do>Gangwon-do part in the slope degrees. The soil texture was of wide distribution on sandy loam soil for Gangwon-do(76%) and Jeollabuk-do part(64%), and 1oam(42%) and sandy loam soil(35%) for Gyeongsangbuk-do part. From the numbers of investigated fields, the distribution of slope degree was wider than those of height above sea level in relation to location surroundings. The upland soils of 785 fields cultivated vegetable crops were sampled at 0~15 cm of top soil before seeding or transplanting and analyzed. On an average, pH, organic matter, available phosphate and exchangeable potassium, calcium, magnesium of soil were 5.7, $27.6g\;kg^{-1}$, $765mg\;kg^{-1}$, $1.16cmol_c\;kg^{-1}$, $6.1cmol_c\;kg^{-1}$, and $1.6cmol_c\;kg^{-1}$, respectively. The average cation exchange capacity(CEC) of 120 sites in Gangwon-do part was $9.2cmol_c\;kg^{-1}$. The content of organic matter, exchangeable potassium, exchangeable calcium and exchangeable magnesium was higher, while that of available phosphate was lower with slope degrees. And the content of major chemical components in carrot soil was lower in comparison with other crop soils. The average levels of N, $P_2O_5$, $K_2O$, livestock manure and lime fertilizer of 785 Belds applied by farmers were 335, 198, 244, 12,680 and $1,750kg\;ha^{-1}$, respectively, for summer vegetable crops in alpine area. The average amounts of $N-P_2O_5-K_2O$ fertilizers applied by farmers in 785 Gelds of vegetable crops were higher 1.7~2.0-4.2~7.0-1.4~2.0 times on potato, 1.4~1.6-4.6~8.3-3.5~4.2 times on Chinese cabbage, and 1.2~1.3-4.2~7.2-3.0~3.61 times on radish than the rates of NPK fertilizers based on soil testing for each crop.

Changes of Nutrient Accumulation Type and Chemical Property on Annual Dressing Paddy Soil in Fluvio-marine deposit (하해혼성 충적층 유기물 연용 논토양의 화학성 및 양분 집적형태 변화)

  • Yang, Chang-Hyu;Yoo, Young-Seok;Yoo, Chul-Hyun;Jung, Ji-Ho;Kim, Jae-Duk;Jung, Kwang-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.6
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    • pp.351-356
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    • 2006
  • A long-term experiment was initiated in 1979 at Experiment Farm of Honam Agricultural Research Institute, to investigate the effects of continued use of organic matter (rice straw and compost) along with different levels of nitrogen fertilizer in rice cultivation. The soils of experimental plots is classified as Jeonbug series developed on Fluvio-marine deposits. The application rates of rice straw and compost were 5 Mg/ha/year and 10 Mg/ha/per year, respectively. The fertilizer N application rates per season were 0, 100, 150, 200, and 250 kg/ha. In 2002, after 24 years of experiment, the effect of different treatments on soil pH, characteristics soil organic matter, soil N and P were investigated. The results of the study is summarized as following. The continue use of organic matter tended to lower the soil pH, to increase organic matter and available phosphate contents. The rice straw tended to lower soil pH more than compost, while the effect of compost was greater in increasing soil organic matter and available soil P then rice straw. The application of organic matter resulted in the increase in total organic N in the soil. Such effect was greater in compost application than in rice straw application. In organic N, greatest was amino acid-N, followed by unidentifiable organic N. The least was amino sugar-N. The application of organic matter with and without nitrogen fertilizer affected the in organic fractions of P, particularly Fe-P and Al-P. The application of rice straw tended to increase Ca-P.

Investigations of Soil Chemical Properties in the Cultivation Fields of Rubus coreanus with Different Growth Ages (복분자 재배 기간에 따른 토양의 화학적 특성 변화)

  • Lee, Jin-Ho;Lee, Chang-Ho;Kang, Byeong-Suk;Ahn, Byung-Koo
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.6
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    • pp.923-929
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    • 2010
  • This study was conducted to investigate the relations between soil chemical properties and Korean raspberry (Rubus coreanus Miq.) growth. Soils were collected from 54 sampling sites in 1 to 6 years old Korean raspberry cultivation fields (RCFs) at Gochang-gun, Jeollabuk-Do, Korea. In RCF with two to six years old plants, soil pH and exchangeable $Ca^{2+}$ and $Mg^{2+}$ contents were higher at the leaf-emergence time, but EC, available phosphorus content, CEC, and total nitrogen content were higher at the harvesting time; especially at the harvesting time, the content of available phosphorus in the RCF with 3~6 years old plants were at least three times higher than in the RCF with 2 years old plants. Water-soluble anions ($Cl^-$, ${NO_3}^-$, ${PO_4}^{2-}$, and ${SO_4}^{2-}$) in the RCF were also measured. The contents of ${NO_3}^-$ and ${SO_4}^{2-}$ were always higher in the RCF with older plants than in the RCF with younger plants, which were not affected by sampling time and sampling locations (rhizosphere and non-rhizosphere sites). However, soils collected from non-rhizosphere at the leaf-emergence time contained higher ${PO_4}^{2-}$ content, but $Cl^-$ content was higher in the soils collected from rhizosphere at the harvesting time. In general, soils in the RCFs contained excess amounts of inorganic nutrients such as available phosphorus and exchangeable $K^+$. Thus, optimal levels of soil properties for Korean raspberry cultivation should be reconsidered.

Effect of Different Levels of Applications of Illite on the Growth of Red Pepper (Capsicum annuum L.) in Bed Soil (상토에서 일라이트의 혼합비율에 따른 고추 육묘시 생육효과)

  • Lee, Seok-Eon;Kim, Hong-Ki;Kwon, Sang-Moon;Kim, Hee-Jung;Yoo, Ri-Bi;Baek, Ki-Tae;Lee, Moon-Soon;Woo, Sun-Hee;Park, Man;Chung, Keun-Yook
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.6
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    • pp.852-857
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    • 2010
  • This study was performed to explore the effect of the clay mineral illite on the improvement of bed soil and plant growth. Red pepper (Capsicum annuum L.) was used as a model vegetable crop. The experiment was performed during the whole six weeks in the glass house of the Chungbuk National University. Its seedlings were cultivated in the bed soil normally used for horticultural purpose. Of the seedlings cultured, the healthy and regular size of seed were selected and cultivated in the pots. They were treated with two forms of illite, particulate (PA) and powder (PW), at the following application rates: standard application[P1 (PA1, PW1), 1:20 (w/w)], two times[P2 (PA2, PW2), 1:10 (w/w)], and four times[P4 (PA4, PW4), 1:5 (w/w)] of standard application. Untreatment (P0) was used as a control pot. At six weeks of cultivation, their growth lengths were correspondingly increased as the application rate was increased ranging from P0, P1, P2, and to P4. Their growth length was a little greater on the application of powder illite (PW) than on the particulate illite (PA). Based on the plant analysis for the root, leaf, stem of red pepper, the uptake amounts of K, Ca, and Mg, were correspondingly increased, as the application rate was increased ranging from P0, P1, P2, and to P4, respectively. At the same application rate, their amounts taken up in the respective parts were higher on the application of PW illite than on the PA one. Especially the amounts of Ca and Mg were higher in the stem, leaf than root. Consequently, it appears that the illite treatment, especially, PW form of illite, enhance the growth of red pepper in the glass house during the whole six weeks of experiment.

Estimation of Nondestructive Rice Leaf Nitrogen Content Using Ground Optical Sensors (지상광학센서를 이용한 비파괴 벼 엽 질소함량 추정)

  • Kim, Yi-Hyun;Hong, Suk-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.6
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    • pp.435-441
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    • 2007
  • Ground-based optical sensing over the crop canopy provides information on the mass of plant body which reflects the light, as well as crop nitrogen content which is closely related to the greenness of plant leaves. This method has the merits of being non-destructive real-time based, and thus can be conveniently used for decision making on application of nitrogen fertilizers for crops standing in fields. In the present study relationships among leaf nitrogen content of rice canopy, crop growth status, and Normalized Difference Vegetation Index (NDVI) values were investigated. We measured Green normalized difference vegetation index($gNDVI=({\rho}0.80{\mu}m-{\rho}0.55{\mu}m)/({\rho}0.80{\mu}m+{\rho}0.55{\mu}m)$) and NDVI($({\rho}0.80{\mu}m-{\rho}0.68{\mu}m)/({\rho}0.80{\mu}m+{\rho}0.68{\mu}m)$) were measured by using two different active sensors (Greenseeker, NTech Inc. USA). The study was conducted in the years 2005-06 during the rice growing season at the experimental plots of National Institute of Agricultural Science and Technology located at Suwon, Korea. The experiments carried out with randomized complete block design with the application of four levels of nitrogen fertilizers (0, 70, 100, 130kg N/ha) and same amount of phosphorous and potassium content of the fertilizers. gNDVI and rNDVI increased as growth advanced and reached to maximum values at around early August, G(NDVI) were a decrease in values of observed with the crop maturation. gNDVI values and leaf nitrogen content were highly correlated at early July in 2005 and 2006. On the basis of this finding we attempted to estimate the leaf N contents using gNDVI data obtained in 2005 and 2006. The determination coefficients of the linear model by gNDVI in the years 2005 and 2006 were 0.88 and 0.94, respectively. The measured and estimated leaf N contents using gNDVI values showed good agreement ($R^2=0.86^{***}$). Results from this study show that gNDVI values represent a significant positive correlation with leaf N contents and can be used to estimate leaf N before the panicle formation stage. gNDVI appeared to be a very effective parameter to estimate leaf N content the rice canopy.

Association of Cognitive Dysfunction with Thyroid Autoantibody (갑상선 자가항체와 인지기능 저하의 연관성)

  • Han, Dong Kyun;Cheon, Jin Sook;Choi, Young Sik;Kim, Ho Chan;Oh, Byoung Hoon
    • Korean Journal of Psychosomatic Medicine
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    • v.24 no.2
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    • pp.227-235
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    • 2016
  • Objectives : The aims of this study were to know the frequency of cognitive dysfunction among patients with autoimmune thyroid disorders, and to reveal influencing factors on it, especially to clarify association with autoimmune thyroid antibodies. Methods : From sixty-five female patients with autoimmune thyroid disorders, demographic data were obtained by structured interview. Their cognitive funtions were measured using the MMSE-K and the MoCA-K tests. Depression was evaluated by the K-HDRS. Results : 1) Among patients with autoimmune thyroid disorders, 7.69% of them were below 24 on the MMSE-K, while 10.77% were below 22 on the MoCA-K. The frequency of cognitive deficit was not significantly different according to having positivity to antimicrosomal antibodies or not. 2) The antimicrosomal antibody-positive patients had significantly higher antithyroglobulin antibody titers, antimicrosomal antibody titers, and TSH concentration, while had significantly lower free T4 levels(p<0.05, respectively). 3) The total scores of the MMSE-K and the MoCA-K had significant correlation with age, marital status, antithyroglobulin antibody titers and K-HDRS(p<0.05, respectively). 4) The regression analysis revealed that variables such as age, education, autoimmune thyroid antibodies, thyroid function and depression did not influence on cognitive function of patients with autoimmune thyroid disorders. Conclusions : Our results could not support that cognitive function of patients with autoimmune thyroid disorders had correlation with autoimmune thyroid antibodies.