• YAKHAK HOEJI
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• v.47 no.6
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• pp.410-416
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• 2003

Autotaxin(ATX) was originally purified from conditioned media of A2058 human melanoma cells and shown to be a potent cell motility-stimulating factor, possessing a type II nucleotide pyrophosphatase/phosphodiesterase (NPP2) activity. Recombinant ATX has recently demonstrated that human plasma lysophosholipase D is identical to ATX and uses lysophosphatidylcholine as a substrate to mediate various biological functions including tumor cell growth and motility through G-protein coupled receptor. However, despite pivotal roles of ATX on physiological or pathophysiological states, the production of ATX is solely depends on complicated purification method which employs multiple column steps, but resulted in very poor yield. This limited the use of ATX for extensive analysis. We, therefore, expressed six histidine-tagged recombinant human ATX(His-ATX) in High Five TM insect cells to improve the generation of ATX and to make simple the purification of ATX. The signal sequence of the human ATX gene was truncated and replaced with sequence of insect cell secretion signal within expression vector. In addition, codons for six histidines were added to the C-termini of 120kDa ATX cDNA construct. A simple purification scheme utilizing two-step affinity column chromatography was designed to purify His-ATX to homogeneity from the culture supernatant of transfected insect cells. Homogenous His-ATX was detected and isolated from the concentrated insect cell medium using concanavalin A agarose and nickel affinity chromatography. Purified His-ATX was in full length with ATX capacity. A combination of this expression system and purification scheme would be useful for production and purification of high-quality functional ATX for research and practical application of multiple functional motogen, ATX/NPP-2.

• Progress in Superconductivity and Cryogenics
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• v.14 no.4
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• pp.20-23
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• 2012

Superconducting equipment has been actively investigated for securing the environment and energy technology (ET) in various parts of the world. Despite these movements, a high voltage cryogenic bushing, which plays an important role of interconnection between the electric power systems and superconducting devices, has not been fully developed due to severe insulation requirements. A gas insulated cryogenic bushing has been investigated as one of our projects since 2010. As a basic step to obtain the design parameters for cryogenic bushing, we focused on the surface flashover characteristics of glass fiber reinforced plastic (FRP) in several insulation gases. For the surface flashover tests, several insulation gases including $SF_6$, $CF_4$ and $N_2$ gas were prepared. Various length of FRP specimens were fabricated in order to obtain the fundamental data for creepage distance of FRP. The first specimen group was from 2 mm to 10 mm with 2 mm intervals and the second specimen group was from 20 mm to 100 mm with 20 mm intervals. And the gas pressure was varied from 1 bar to 4 bar. An AC overvoltage test and a lightning impulse test were performed. Then the experimental results of surface flashover were obtained and analyzed. Based on these results, it would be possible to design the optimum creepage distance of FRP in a cryogenic bushing.

• International Journal of Fluid Machinery and Systems
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• v.3 no.4
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• pp.315-323
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• 2010

This work investigates the influence of water compressibility on pressure pulsations induced by rotor-stator interaction (RSI) in hydraulic machinery, using the commercial CFD solver ANSYS-CFX. A pipe flow example with harmonic velocity excitation at the inlet plane is simulated using different grid densities and time step sizes. Results are compared with a validated code for hydraulic networks (SIMSEN). Subsequently, the solution procedure is applied to a simplified 2.5-dimensional pump-turbine configuration in prototype with different speeds of sound as well as in model scale with an adapted speed of sound. Pressure fluctuations are compared with numerical and experimental data based on prototype scale. The good agreement indicates that the scaling of acoustic effects with an adapted speed of sound works well. With respect to pressure fluctuation amplitudes along the centerline of runner channels, incompressible solutions exhibit a linear decrease while compressible solutions exhibit sinusoidal distributions with maximum values at half the channel length, coinciding with analytical solutions of one-dimensional acoustics. Furthermore, in compressible simulation the amplification of pressure fluctuations is observed from the inlet of stay vane channels to the spiral case wall. Finally, the procedure is applied to a three-dimensional pump configuration in model scale with adapted speed of sound. Normalized Pressure fluctuations are compared with results from prototype measurements. Compared to incompressible computations, compressible simulations provide similar pressure fluctuations in vaneless space, but pressure fluctuations in spiral case and penstock may be much higher.

• Journal of Surface Science and Engineering
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• v.52 no.6
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• pp.298-305
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• 2019

We investigated a correlation between morphology and photoelectrochemical properties of TiO₂ nanotubes fabricated by well-controlled anodization processes. Anodization in an ethylene-glycol-based electrolyte solution accelerated the rapid grow rate of TiO₂ nanotubes, but also cause problems such as delamination at the interface between TiO₂ nanotubes and a Ti substrate, and debris on the top of the nanotube. The applied voltages for the anodization of TiO₂ were adjusted to avoid the interface delamination. The heat treatment and the anodizing time were also controlled to enhance the crystallinity of the as-prepared TiO₂ nanotubes and to increase the surface area with the varied length of the anodized TiO₂ nanotubes. Additionally, a 2-step anodization process was utilized to remove the debris on the tube top. The photoelectrochemical properties of TiO₂ nanotubes prepared with the carefully tailored conditions were investigated. By removing the debris on TiO₂ nanotubes, applied bias photon-to-current efficiency (ABPE) of TiO₂ nanotubes increased up to 0.33%.

• Proceedings of the KIEE Conference
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• 2003.07d
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• pp.2372-2374
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• 2003

With 3-D vision measuring, camera calibration is necessary to calculate parameters accurately. Camera calibration was developed widely in two categories. The first establishes reference points in space, and the second uses a grid type frame and statistical method. But, the former has difficulty to setup reference points and the latter has low accuracy. In this paper we present an algorithm for camera calibration using perspective ratio of the grid type frame with different line widths. It can easily estimate camera calibration parameters such as focal length, scale factor, pose, orientations, and distance. But, radial lens distortion is not modeled. The advantage of this algorithm is that it can estimate the distance of the object. Also, the proposed camera calibration method is possible estimate distance in dynamic environment such as autonomous navigation. To validate proposed method, we set up the experiments with a frame on rotator at a distance of 1,2,3,4[m] from camera and rotate the frame from -60 to 60 degrees. Both computer simulation and real data have been used to test the proposed method and very good results have been obtained. We have investigated the distance error affected by scale factor or different line widths and experimentally found an average scale factor that includes the least distance error with each image. It advances camera calibration one more step from static environments to real world such as autonomous land vehicle use.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.8 no.4
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• pp.842-847
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• 2007

In this study, for the purpose of estimating the resisting moment of sloped ground based on level ground fall down safety equation in design specification, compute it depends on penetration depth of concrete pile applying modulus of foundation about the angle of internal friction, cohesion, unit weight of soil, classes of the ground, sandy or clay soil, and verify established study using L-Pile Plus13.8. Also, select four cases that characteristics of soil depending on the soil grade is considered and compute the 12m length concrete pile's resisting moment of the ground those angle is changing from $0^{\circ}{\sim}35^{\circ},\;step\;5^{\circ}$. In the result, identify that the resisting moment of ground decreases depending on ground slope. Thus, increasing of penetration depth is required.

• Proceedings of the Korean Vacuum Society Conference
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• 2000.02a
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• pp.64-64
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• 2000

Since its discovery in 1991, the carbon nanotube has attracted much attention all over the world; and several method have been developed to synthesize carbon nanotubes. According to theoretical calculations, carbon nanotubes have many unique properties, such as high mechanical strength, capillary properties, and remarkable electronical conductivity, all of which suggest a wide range of potential applications in the future. Here we report the synthesis in the catalytic decomposition of acetylene at ~65 $0^{\circ}C$ over Ni deposited on SiO2, For the catalyst preparation, Ni was deposited to the thickness of 100-300A using effusion cell. Different approaches using porous materials and HF or NH3 treated samples have been tried for synthesis of carbon nanotubes. It is decisive step for synthesis of carbon nanotubes to form a round Ni particles. We show that the formation of round Ni particles by heat treatment without any pre-treatment such as chemical etching and observe the similar size of Ni particles and carbon nanotubes. Carbon nanotubes were synthesized by chemial vapour deposition ushin C2H2 gas for source material on Ni coated Si substrate. Ni film gaving 20~90nm thickness was changed into Ni particles with 30~90nm diameter. Heat treatment of Ni fim is a crucial role for the growth of carbon nanotube, High-resolution transmission electron microscopy images show that they are multi-walled nanotube. Raman spectrum shows its peak at 1349cm-1(D band) is much weaker than that at 1573cm-1(G band). We believe that carbon nanotubes contains much less defects. Long carbon nanotubes with length more than several $\mu$m and the carbon particles with round shape were obtained by CVD at ~$650^{\circ}C$ on the Ni droplets. SEM micrograph nanotubes was identified by SEM. Finally, we performed TEM anaylsis on the caron nanotubes to determine whether or not these film structures are truly caron nanotubes, as opposed to carbon fiber-like structures.

• Journal of Korean Neurosurgical Society
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• v.29 no.4
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• pp.528-535
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• 2000

Objective : To investigate objectively the postoperative improvement of gait disturbance in patients with cervical myelopathy through a gait analysis. Patients and Methods : Ten patients who underwent cervical decompression and fusion for cervical myelopathy caused by spondylosis, OPLL, or concomitant hypertrophy of ligamentum flavum were studied. Preoperatively, gait disturbance was present in all patients. The patients were evaluated by gait analysis using three dimensional motion analyzer to collect data of linear and kinematic parameters before surgery, 1 week and 3 months after surgery. Statistical analysis of the related pre-and post-operative data were performed. Results : In the linear parameters, average value of cadence, walking speed, stride length, step time, width and double support were increased postoperatively compare to preoperative value. In the kinematic parameters, average value of knee flexion during initial swing phase, plantar flexion of ankle and range of motion of hip joint were increased as well. These differences were statistically significant(p<0.05). Conclusion : This study suggests that gait analysis can be used as a method of quantitative analysis of postoperative gait improvement in patients with cervical myelopathy.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.188-188
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• 2016

The Mekong which is one of the world's most significant rivers plays an extremely important role to South East Asia. Lying across six riparian countries including China, Myanmar, Thailand, Laos, Cambodia and Vietnam and being a greatly biological and ecological diversity of fishes, the river supports a huge population who living along Mekong Basin River. Therefore, much attention has been focused on the giant Mekong Basin River, particularly, the soil erosion and sedimentation problems which rise critical impacts on irrigation, agriculture, navigation, fisheries and aquatic ecosystem. In fact, there have been many methods to calculate these problems; however, in the case of Mekong, the available data have significant limitations because of large area (about 795 00 km2) and a failure by management agencies to analyze and publish of developing countries in Mekong Basin River. As a result, the Universal Soil Loss Equation (USLE) model in a GIS (Geographic Information System) framework was applied in this study. The USLE factors contain the rainfall erosivity, soil erodibility, slope length, steepness, crop management and conservation practices which are represented by raster layers in GIS environment. In the final step, these factors were multiplied together to estimate the soil erosion rate in the study area by using spatial analyst tool in the ArcGIS 10.2 software. The spatial distribution of soil loss result will be used to support river basin management to find the subtainable management practices by showing the position and amount of soil erosion and sediment load in the dangerous areas during the selected 56- year period from 1952 to 2007.

• Smart Structures and Systems
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• v.7 no.4
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• pp.303-317
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• 2011

A specially designed tendon, which is proposed by embedding an FBG sensor into the center king cable of a 7-wire strand tendon, was applied to monitor the prestress force and load transfer of ground anchor. A series of tensile tests and a model pullout test were performed to verify the feasibility of the proposed smart tendon as a measuring sensor of tension force and load transfer along the tendon. The smart tendon has proven to be very effective for monitoring prestress force and load transfer by measuring the strain change of the tendon at the free part and the fixed part of ground anchor, respectively. Two 11.5 m long proto-type ground anchors were made simply by replacing a tendon with the proposed smart tendon and prestress forces of each anchor were monitored during the loading-unloading step using both FBG sensor embedded in the smart tendon and the conventional load cell. By comparing the prestress forces measured by the smart tendon and load cell, it was found that the prestress force monitored from the FBG sensor located at the free part is comparable to that measured from the conventional load cell. Furthermore, the load transfer of prestressing force at the tendon-grout interface was clearly measured from the FBGs distributed along the fixed part. From these pullout tests, the proposed smart tendon is not only expected to be an alternative monitoring tool for measuring prestress force from the introducing stage to the long-term period for health monitoring of the ground anchor but also can be used to improve design practice through determining the economic fixed length by practically measuring the load transfer depth.