• Journal of Oral Medicine and Pain
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• v.30 no.2
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• pp.141-148
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• 2005

Since many reports for the influence of the essential oils on the oral microorganism were presented, it is important to use the essential oils in clinical field. At the present day which emphasized the quality of life, oral malodor is one of the serious problems, so we need to try to decrease of oral malodor. This study was designed to make on evidence of the effect of essential oils to oral malodor clinically and to develop on effective treatment for oral malodor by using the dental paste which contains essential oils. 40 dental students volunteered to participate in this study and double blind test was used. At the beginning, all subjects were measured their morning malodor prior to this experiment, at the 3cm posterior to their incisal edge of the oral cavity by Halimeter(Interscan Co. Chatsworth, CA). After that we divided the students in half into two groups A and B. We have supplied different tooth pastes, dental paste A and B, to two groups respectively. The dental paste B contained essential oils which are tea tree, lemon and peppermint. All students used different tooth pastes everyday for 3 weeks. And all students were measured their morning malodor every week with the same method by using Halimeter. This study was resulted that ; 1. The dental paste which contains essential oils has continuously decreased the morning malodor during the whole period of experiments. 2. The number of students with increase in their morning malodor was generally decreased in the group B who used the toothpaste which contains essential oils, compared with the group A who used the toothpaste which didn't contain essential oils on the 2nd and 3rd week of the experiment. 3. In the group B, the average data on increasing rate of morning malodor was less than in the group A. 4. In the 2nd and 3rd week of the experiment, the data on the rate of morning malodor of the group B was generally decreased continuously compared with the group A. These results have statistically significance under 95% confidence interval of the difference.(p$\leq$0.05) As the result, the toothpaste which was containing essential oils was effective in decreasing the morning malodor.

• The Korean Journal of Pesticide Science
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• v.15 no.4
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• pp.350-356
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• 2011

Environment-friendly agro-materials tend to be preferred to chemical insecticides recently. For this reason, many studies were conducted to develop environment-friendly insecticides containing natural materials. The purpose of this study was to assess ecotoxicity for 5 plant essential oils (Thyme white, Clove bud, Cassia, Lavender, Lemon eucalyptus) expected to prevent from pests and be used for agro-materials. Target species used to assess acute toxicity were aquatic invertebrate (Daphina magna), fish (Oryzias latipes), honeybee (Apis mellifera L.) and earthworm (Eisenia fetida). The EC50 value, toxicological responses of thyme white, clove bud, and cassia to Daphina magna were 2.5, 2.8, and $6.9mg\;L^{-1}$ respectively and these values were moderately toxic according to standard of USEPA. $EC_{50}$ of Lavender and lemon eucalyptus were >$10mg\;L^{-1}$ then they were considered as slightly toxicity. In case of acute toxicity test to fish, $LC_{50}$ of thyme white and cassia were 6.7 and $7.5mg\;L^{-1}$ each other. The other plant essential oils indicated $LC_{50}$ >$10mg\;L^{-1}$. Acute contact and oral toxicity test to Honeybee were conducted. As a result, $LD_{50}$ of all essential oils were >$100{\mu}g$ a.i. $bee^{-1}$ in both of tests. In case of acute toxicity test to earthworm, $LC_{50}$ of thyme white, clove bud, cassia, lavender, and lemon eucalyptus were 149, 230, 743, 234, and $635mg\;kg^{-1}$, respectively. In conclusion, if the safety for earthworm is confirmed, 5 plant essential oils are expected to be use for environment-friendly insecticide materials with low risk against ecosystem and contribute to developing environment-friendly agro-materials.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.6
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• pp.261-271
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• 2019

This study was conducted to investigate the effect of aromatherapy on the autonomic nervous system by the inhalation. The subjects of the study were 64 people aged 20 and under 59 years old, 32 in the experimental group and 32 in the control group. Blending oil with Six aroma(Lavender, Bergamot, Mandarin, Lemon, Cedarwood, Roman Chamomile) was applied to the experimental group, and Jojoba oil was applied to the control group for 30min. Before and after the experiment, HRV(TP, VLF, LF, HF, LF/HF) of the experimental group and the control group were compared and analyzed using pulse wave mesuring instrument(uBioClip v70). There was a significant difference between the experimental group and the control group in 3 out of 5 items analyzed TP(p<.05), LF(p<.05), HF(p<.01). In other words, aromatherapy increases the total activity of the autonomic nervous system by enhancing the TP, increases the activity of the sympathetic and parasympathetic nerves, promotes the activity of the heart, Facilitate the activity of the heart. Therefore, if aromatherapy is used steadily in everyday life, it will enable healthy life by positively affecting the autonomic nervous system response.

• Food Science and Preservation
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• v.24 no.3
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• pp.394-405
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• 2017

This study was conducted to confirm the usefulness of essential oil components in yuzu and kumquat cultivated in Korea for comparison with those in lemon and lime. The volatile flavor compounds in citrus fruits (yuzu, kumquat, lemon and lime) were extracted for 3 h with 100 mL redistilled n-pentane/diethylether (1:1, v/v) mixture, using a simultaneous steam distillation and extraction apparatus (SDE). The volatile flavor compositions of the samples were analyzed by gas chromatography-mass spectrometry (GC-MS). The aroma compounds analyzed were 104 (3,713.02 mg/kg) in yuzu, 87 (621.71 mg/kg) in kumquat 103 (3,024.69 mg/kg) in lemon and 106 (2,209.16 mg/kg) in lime. Limonene was a major volatile flavor compound in four citrus fruits. The peak area of limonene was 35.03% in yuzu, 63.82% in kumquat, 40.35% in lemon, and 25.06% in lime. In addition to limonene, the major volatile flavor compounds were ${\gamma}$-terpinene, linalool, ${\beta}$-myrcene, (E)-${\beta}$-farnesene, ${\alpha}$-pinene and ${\beta}$-pinene in yuzu, and ${\beta}$-myrcene, ${\alpha}$-pinene, (Z)-limonene oxide, (E)-limonene oxide, geranyl acetate and limonen-10-yl acetate in kumquat. Furthermore, ${\gamma}$-terpinene, ${\beta}$-pinene, ${\beta}$-myrcene, geranyl acetate, neryl acetate and (Z)-${\beta}$-bisabolene in lemon and ${\gamma}$-terpinene, ${\beta}$-pinene, (Z)-${\beta}$-bisabolene, neral, geranial and neryl acetate in lime were also detected. As a result, it was confirmed that the composition of volatile flavor compounds in four citrus fruits was different. Also, yuzu and kumquat are judged to be worthy of use alternatives for lemon and lime widely used in the fragrance industry.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.5
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• pp.2206-2211
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• 2012

To identify the effect of the aroma foot spa on the stress, 12 university students with stress were divided into control group (6 subjects) and aroma foot spa group (6 subjects). Control group was not subjected to any kind of intervention while aroma foot spa group was subjected to 10 minutes of foot bath using 5% Juniper, lavender and lemon essential oil and 10 minutes of foot massage using massage cream during four weeks, two times per week. As for the evaluation method, brain wave was studied to measure the amount of change in stress. After measuring the change in the brain wave before and after the experiment, it was proven that the aroma foot spa group s Alpha wave (Z=-2.364, p<.05) and SMR wave (Z=-1.981, p<.05) were higher than those of the control group. Moreover, when the pre and post experiment results of the aroma foot spa group were measured, it was proven that the Theta wave (Z=-2.366, p<.05) decreased while Alpha wave (Z=-2.371, p<.05) increased. In other words, aroma foot spa that included foot bath using essential oil and foot massage increased Alpha wave which in turn influenced the brain wave due to increased blood circulation resulting from muscle relaxation. Moreover, SMR wave increase was closely related to the change in Alpha wave, which demonstrates that SMR wave increased due to stress alleviation. Accordingly, it may be possible to assume that aroma foot spa is effective in relieving stress.

• The Korean Journal of Food And Nutrition
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• v.18 no.1
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• pp.48-53
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• 2005

Herbs and yucca(Yucca shidigera) are widely used a source of tea and otherwise such as botanical medicine, essential oil for perfumes, cosmetics, and food spices. This study was carried out to investigate the characteristics of herb tea prepared with lemon verbena, spearmint, apple mint, Korea mint, rosemary, pineapple sage, Chrysanthemum indicum, stevia, lavender, lemon balm cultivated in Korea and effects by addition of yucca. Approximate composition of yucca were as follows: moisture 5.88%, crude protein 5.46%, crude fat 0.69%, crude ash and 7.59%. pH of herb tea mixed yucca at 80℃ was decreased in lemon verbena, spearmint, apple mint, Korea mint, and rosemary in comparison herb tea alone, but soluble solids were similar in two tea. In the result of sensory test, herb tea and herb tea mixed Yucca were not difference with a few exceptions. pH of mentha(Korea mint) tea by addition of yucca was decreased according to increment amount of yucca. The sensory evaluation score of mentha tea by addition of yucca were increased when the tea was processed with 0.01∼0.04% yucca.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.17 no.4
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• pp.539-547
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• 2010

Purpose: This study was done to determine the effects of aromatherapy on anxiety and discomfort for patients who were having colonoscopy. Methods: The study used a nonequivalent control group non-synchronized design. The participants were 48 people who were undergoing colonoscopy in a university hospital. The experimental group inhaled a blend of essential oil with neroli, camomile, lavender and lemon through an aroma stone for 5minutes before the examination. Then the aroma stone was put on the side of the pillow to spread the aroma scent in the room. The experimental group could inhale the aroma scent as soon as they came into the room and during the examination process. The control group received only fundamental nursing care. Results: Aromatherapy significantly decreased VAS anxiety. There were also significant differences in facial change and, tone change for objective discomfort. But there were no significant differences in subjective discomfort between the groups nor were there any significant differences in vital signs. Conclusion: Aromatherapy was perceived as an useful intervention to reduce anxiety and objective discomfort of patients receiving colonoscopy. The results of this study could be utilized as a clinical nursing intervention.

• The Korean Journal of Pesticide Science
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• v.16 no.4
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• pp.376-382
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• 2012

Environment-friendly agro-materials are are likely to be preferred to chemical insecticides recently. For this reason, many studies are conducted to develop environment-friendly insecticides containing natural materials. This study was also conducted so as to assess ecotoxicity for Emulsifiable concentrate (EC) containing 30% of garlic extract or two plant essential oils (Zanthoxylum, Lemongrass) expected to prevent from pests and be used for agro-materials. Target species used to assess acute toxicity were invertebrate (Daphina magna), fish (Oryzias latipes), honeybee (Apis mellifera L.) and earthworm (Eisenia fetida). The $EC_{50}$ values for of garlic extract 30% EC, Zanthoxylum oil 30% EC and lemongrass oil 30% EC to Daphina magna were 3.3, 10, and $10mg\;L^{-1}$, respectively. The category of garlic extract 30% EC was moderately toxic, while those of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were slightly toxic according to standard of USEPA. $EC_{50}$ for both of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were more than $10mg\;L^{-1}$ then they were considered as slightly toxicity. In case of acute toxicity test to fish, $LC_{50}$ of garlic extract 30% EC was $3.3mg\;L^{-1}$. Zanthoxylum oil 30% EC and lemongrass oil 30% EC indicated $LC_{50}$ > $10mg\;L^{-1}$. Classification of acute toxicity to all test substances was in Korea criteria. Acute contact and oral toxicity test to Honeybee were conducted. As a result, $LD_{50}$ of all test substances were more than 100 a.i. ${\mu}g\;bee^{-1}$ in the acute contact test while $LD_{50}$ of garlic extract 30% EC was 4.4 a.i. ${\mu}g\;bee^{-1}$ and $LD_{50}$ of Zanthoxylum oil 30% EC and lemongrass oil 30% EC were more than 100 a.i. ${\mu}g\;bee^{-1}$. In case of acute toxicity test to earthworm, $LC_{50}$ of garlic extract 30% EC, Zanthoxylum oil 30% EC and lemongrass oil 30% EC were 267, 592, and $430mg\;kg^{-1}$, respectively. In conclusion, if the safety for earthworm is confirmed, these substances are expected to be use for environment-friendly insecticide materials with low risk against ecosystem and contribute to developing environment-friendly agro-materials.

• Journal of Life Science
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• v.23 no.5
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• pp.637-642
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• 2013

This study was performed to evaluate the rodent repellency and antimicrobial activity of natural oils. Thirteen natural essential oils were evaluated for repellent efficacy against rodent feed intake suppression activity. Salvia sclarea (sage), Cinnamomum zeylanicum (cinnamon) Cymbopogon citratus (lemon grass), and Syzygium aromaticum (clove) showed the best repellent efficacy among tested natural oils. Antimicrobial activity against Gram-negative (four strains) and Gram-positive (four strains) bacteria were evaluated using four natural oils, which showed high efficacy on the rodent repellent test. Syzygium aromaticum showed antimicrobial activity against all tested bacteria and Cymbopogon citratus, Cinnamonum zeylanicum, and Salvia sclarea showed antimicrobial activity against some tested bacteria.

• Stress
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• v.25 no.4
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• pp.239-245
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• 2017

Background: A quasi-experimental study with non-equivalent control groups was performed to determine an effect of aromatherapy foot bath on stress and autonomic nervous activity. Methods: To confirm the effect of aromatherapy foot bath, aromatherapy foot bath group was asked to have a bathing by dropping 0.5 cc in 18 L water after blending with a ratio of 6:3:1 with essential oil lavender, lemon, and tea tree and a foot bath group was asked for bathing only by water. Results: Aromatherapy foot bath group showed significant decrease in subjective stress score (t=3.465, p=0.001) and stress index (t=3.021, p=0.004) in addition to simultaneous increase in sympathetic nervous activity (t=-2.913, p=0.005). However, no significant stimulation of parasympathetic nervous system was observed (t=-1.831, p=0.072). Conclusions: As aromatherapy foot bath is a possible effective intervention for stress relief, it can be applied as an effective method to relieve the stress for healthy adults and patients.