• Journal of Animal Environmental Science
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• v.15 no.3
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• pp.263-270
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• 2009

A study was conducted to determine the effects of seed mixture and nitrogen application levels on botanical composition and forage productivity in grazing pasture. Legume forage have the ability to take nitrogen from the atmosphere and convert it into a form usable by plants. Including legumes in mixtures with grass lowers the amount of nitrogen fertilizer required to produce forages. Dry matter (DM) yield of grass-legume mixtures was more than that of grass mono-cultivated and grass+white clover+red clover mixtures was the highest as 17,391 kg/ha in legume mixture (P<0.01). The highest DM yield was in N-200 kg/ha, but it was similar between N-100 kg/ha(15,128) and N-200 kg/ha (16,017). The large decline in the proportion of grass during April to June in 2004 probably was due to the drought and summer depression in grass-legume mixtures. Grass proportions decreased by 8.2% after 2 year, and white clover was dominated in grass-white clover mixture by May 2005. Crud protein (CP) content tends to increase with increased nitrogen application level.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.1
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• pp.66-74
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• 2008

Two experiments were conducted to assess the effect of replacing a conventional concentrate with mixed cassava (Manihot esculenta) foliage and legume (Phaseolus calcaratus) foliage. In Exp. 1, three rumen fistulated crossbred cows were used for in sacco rumen degradability studies. In vitro gas production was also studied. In Exp. 2, 11 crossbred F2 heifers (Red Sindhi$\times$Holstein Friesian), with initial live weight of $129{\pm}6kg$ and aged six months, were allocated in a Completely Randomized Design (CRD) to evaluate a mixture (ratio 3:1) of cassava and legume foliage (CA-LE feed) as a protein source compared to a traditional concentrate feed (Control) in diets based on fresh elephant grass (Pennisetum purpureum) and urea treated rice straw ad libitum. The Control feed was replaced by the CA-LE feed at levels of 0% (Control), 40% (CA-LE40), and 60% (CA-LE60) based on dry matter (DM). The in sacco degradation of CA-LE feed was higher than Control feed (p<0.05). After 48 h incubation the degradation of CA-LE feed and Control feed was 73% vs. 58% of DM and 83% vs. 65% of CP, respectively. The gas production of CA-LE feed was also significantly higher than of Control feed during the first 12 h of incubation. The results of the performance study (Exp. 2) showed that the level of CA-LE feed in the concentrate had no effect on total dry matter intake (p>0.05), but live weight gains (LWG) in CA-LE40 and CA-LE60 were significantly higher (551 and 609 g/d, respectively) than in the Control group (281 g/d). The intake of CP was higher (p<0.05) for the treatments CA-LE40 and CA-LE60 (556 and 590 g/d, respectively) compared to that of Control (458 g/d), while there was no significant difference in ME intake. The feed conversion ratio was 16.8, 9.0 and 7.9 kg DM/kg LWG in Control, CA-LE40 and CA-LE60, respectively. The feed cost of CA-LE40 and CA-LE60 corresponded to 43% and 35%, respectively, of the feed cost of Control feed. The best results were found when CA-LE feed replaced 60% of DM in Control feed and considerably decreased feed cost. It is concluded that feeding cassava foliage in combination with Phaseolus calcaratus legume as a protein supplement could be a potentially valuable strategy which leads to reduced feed costs and a more sustainable system in smallholder dairy production in Vietnam.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.6
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• pp.800-807
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• 2019

Objective: The aim was to evaluate backgrounding beef steers on oat + ryegrass pastures mixed with vetch and/or using energy supplementation. Methods: A randomized block design with three treatments and three replications was used. The treatments were: grass + supplement (oat + ryegrass + supplementation), legume + supplement (oat + ryegrass + vetch + supplementation) and grass + legume (oat + ryegrass + vetch). A continuous grazing system with a variable stocking rate was used. Twenty-seven intact crossbred steers (1/4 Marchigiana, 1/4 Aberdeen Angus and 2/4 Nellore) aged 7 months old and average weight of 190 kg were used. Steers were supplemented at 1% of the body weight of ground corn. The experiment lasted 84 days, between May and August 2014. Behavioral assessments were performed two times per experimental period, for 24 hours. Results: The forage mass was different between treatments, being greater for steers fed without legume. The accumulation rate, forage allowance, and stocking rate did not differ between treatments due to the adequate adjustment of forage allowance. The final weight of animals, as well as the dry matter intake (kg/d), did not differ between treatments. However, forage intake was higher for non-supplemented animals in relation to supplemented steers. Supplement intake did not alter the total digestible nutrient intake due to pasture quality. Animals fed grass + supplement had higher live weight gain per area than those fed grass + legume. Animals without supplementation spent more time in grazing. Conclusion: Feeding behavior was not altered by mixing with vetch or supplementation. Non-supplemented animals started the grazing peak earlier and spent more time in grazing than those supplemented; however, the average daily gain was similar between treatments. The live weight gain per hectare was 47% higher in pastures in which the animals received supplementation compared with those mixed with vetch, a consequence of the substitutive effect.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.4
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• pp.318-324
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• 2016

In order to study the effect of barley, Italian ryegrass (IRG), and legume mixture on nitrogen fixation and transfer to grasses on spring paddy field, an experiment was carried out from Oct. 2006 to June 2007 in Naju, Korea. A split plot design with three replications was used for the experiment. One reference plot was assigned for each treatment to determine nitrogen fixation. Main plots consisted of Chinese milk vetch, crimson clover, forage pea, and hairy vetch with barley, respectively. Subplot treatment were barley or IRG with four seeding ratio of legumes (50:50, 60:40, 70:30, and 80:20). To estimate N fixation by legumes, $^{15}N$ isotope dilution technique was used. $^{15}N$ fertilizer [$(^{15}NH_4)_2SO_4$ solution at 99.8 atom N] was uniformly applied to $600cm^2$ in the middle of each plot on April 15, 2007. Plots were harvest by hand on June 8, 2007. Dried sample were ground to a fine power and analyzed for total N isotope N. $^{15}N$ was determined using elemental analyzer-isotope ratio mass spectrometry. The calculation of N transfer was determined with the isotope dilution method. The content of N was higher in legumes than that in barley or Italian ryegrass. Nitrogen level in forage pea was significantly higher than that of other legumes. There were significantly differences in N content between legumes in IRG mixture. Atom % $^{15}N$ excess was significantly different in legumes with barley. The 60:40 sub plot had higher (p<0.05) atom % $^{15}N$ than other seeding ratio treatments. The enrichment ranged from 0 to 0.58. Compared to barley, the enrichment of IRG with its accompanied legumes was higher, ranging from 0.38 to 1.0. The N derived from the atmosphere (Ndfa) ranged from 0% to 49.5% with barley-legume mixture. It ranged from 0 to 60.5% in IRG-legume plots. N transfer from legumes to neighboring grasses was 12.3 to 90.9 kg/ha for barley-legume mixture and 31.7 to 107.8 kg/ha for IRG plots. IRG plots showed higher N transfer for IRG-legume mixture in general based on difference method. Based on $^{15}N$ dilution method, the N transfer was 0 to 36.1 kg/ha for barley-legume mixture and 0 to 50.6 kg/ha for IRG plots. There was a tendency toward higher N transfer on the difference method than that of the $^{15}N$ dilution method.

• Korean Journal of Organic Agriculture
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• v.23 no.3
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• pp.583-593
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• 2015

This study was conducted to investigate the effects of mixed-sowing of legume forage and applying of cattle manure on the productivity of organic rye to provide dairy farmers with safe organic feeds. Also the present study aimed to evaluate optimal applying level of cattle manure and carrying capacity of Korean native cattle (Hanwoo) per unit area. The amount of crude protein was significantly higher in the mixed-sowing of legume forage (average 760~786 kg/ha) than in the single-sowing of rye (average 353 kg/ha) (p<0.05). The mixed-sowing of forage peas and the single-sowing of rye tended to improve by increasing the level of nitrous fertilization rather than the fertilization treatment. The amount of crude protein on the rye according to the mixed-sowing of legume was significantly higher in the mixture (average 8.29~9.90%) than in the single (average 4.93%) (p<0.05). The comparison by the level of nitrogen fertilization indicated significantly high for the rye in 50 kg N/ha than in fertilization treatments (p<0.05). Total digestible nutrients (TDN) amount in the single-sowing of rye was average 46.86% and has lower than average 49.96~50.12% of the mixed-sowing of legume, and especially the mixed-sowing of forage pea was the highest with 54.55% in 150 kg N/ha for the level of nitrogen fertilization (p<0.05). The breeding ability of annual organic livestocks per unit area according to the feed value of rye presented significantly higher ability in the mixed-sowing of legume (3.72~4.12 heads) than the single-sowing of rye (average 2.26 heads) (p<0.05). By summarizing above results, the mixed-sowing of legume is required to improve the productivity and the feed value of rye for increasing organic livestock breeding ability in southern area of Gyeongbuk regions, and the study for identifying the appropriate fertilization level using livestock excretions is further necessary.

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.57.1-57
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• 2000

No Abstract, See Full Text

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.04a
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• pp.77-84
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• 2003

Metabolic engineering for production of isoflavones in non-legume plants could distribute the health benefits of these phytoe-strogens in more widely-consumed grains. We investigate the ability of the heterologous isoflavone synthase enzyme to interact with the endogenous phenylpropanoid pathway. Overall, results provide possibility of production of isoflavonoids in several plant tissue systems including soybean and non-legumes. In tissue that undergoes naturally enhanced synthesis of anthocyanins, genistein production was enhanced. In a monocot cell system, introduced expression of a transcription factor regulating genes of the antho-cyanin pathway was effective in conferring the ability produce genistein in the presence of the isoflavone synthase gene. However, in this case the intermediate accumulated to high levels indicating an inefficiency in its conversion. Introduction of a third gene, chalcone reductase, provided the ability to synthesize an additional substrate of isoflavone synthase resulting in production of the isoflavone daidzein. These research efforts provide insight into requirements for metabolic engineering for isoflavone production in non-legume dicot and monocot tissues.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.2
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• pp.191-195
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• 1993

The application of fertilizer P to Desmodium intortum at 40, 60 and 100 kg/ha resulted in the P content of the legume regrowth being 0.10, 0.13 and 0.16% on a dry matter basis. When the forages were fed alone to sheep in metabolism pens the corresponding voluntary intakes by sheep of the three forages were 27.3, 42.7 and $54.3g/W_{kg}{^{0.75}}$, respectively. Provision of additional P by rapid infusion into the rumen each morning before any fresh food was offered depressed the voluntary intake of all three feeds. It is suggested that the adverse effect of supplementary P was probably caused by an induced deficiency of either Ca or Mg and led to a reduction in microbial activity in the rumen.

• 한국생태학회:학술대회논문집
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• 2002.08a
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• pp.93-100
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• 2002

Dynamics of certain $N_2$fixing bacteria such as Rhizobium, Azospirillum and Azotobactor, nodule number in dominant legume, Atylosia trinervia, P-solubilizing bacteria, actinomycetes and fungi were studied in unburned and burned site of natural grassland, southern India. Population of $N_2$- fixing bacteria, P-solubilizing bacteria, fungi and nodule number in legume increased significantly in burned sites. On the other hand, the actinomycetes population remained unchanged. Thirty six species of fungi with tricalcium phosphate solubilizing ability were recorded. The most efficient P-solubilizing fungi recognised in the soils of the study sites are Absidia ramosa, Gongronella butlerii, Mortieralla spinosa, Mucor racemosus, Rhizopus nigricans, R. stolonifer, R. oryzae, Aspergillus fumigatus, A. nidulans, A. niger Theilavia terricola and Cheatomium lunasporium.

• The Plant Pathology Journal
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• v.20 no.1
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• pp.18-21
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• 2004

The root hair provides a major entering spot for the symbiotic legume rhizobia. It is obvious that dynamic cell wall modification occurs in the plant root hair during the early microbe invasion. Expansins are nondestructive cell wall-modifying proteins that are involved in cell growth and differentiation. Among about 40 expansin genes in Arabidopsis, two expansin genes are expressed specifically in the root hair cell. Orthologous genes of this Arabidopsis root hair expansins have been found in other Brassica members, rice, and Medicago truncatula (a legume). In this review, I discuss the probable function of expansins during the early symbiotic process between the root hair and microbes and the regulation of root hair expansin genes in a comparative approach.