• Journal of Yeungnam Medical Science
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• v.19 no.1
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• pp.55-62
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• 2002

Background: Interleukin-1(IL-1) and neutrophil appear to contribute to the pathogenesis of acute respiratory distress syndrome(ARDS). Elastase, as well as reactive oxygen species released from activated neutrophil, are thought to play pivotal roles in the experimental models of acute lung leak. This study investigated whether ICI 200,355, a synthetic elastase inhibitor, can attenuate acute lung injury induced by IL-1 in rats. Materials and Methods: We intratracheally instilled either saline or IL-1 with and without treatment of ICI 200,355 in rats. Lung lavage neutrophils, lung lavage cytokine-induced neutrophil chemoattractant(CINC) concentration, lung lavage protein concentration, lung myeloperoxidase(MPO) activity and lung leak index were measured at 5 hours of intratracheal treatment. Results: In rats given IL-1 intratracheally, lung lavage neutrophils, lung lavage CINC concentration, lung lavage protein concentration, lung MPO activity and lung leak index were higher. Intratracheal ICI 200,355 administration decreased lung lavage neutrophils, lung MFO activity and lung leak index, respectively, but did not decrease lung lavage CINC concentration. Conclusion: These results suggest that ICI 200,355 decreases lung inflammation and leak without decreasing lung lavage CINC concentration in rats given IL-1 intratracheally.

• The Korean Journal of Physiology and Pharmacology
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• v.3 no.3
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• pp.263-273
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• 1999

The role of phospholipase $A_2\;(PLA_2)$ in acute lung leak induced by intestinal ischemia was investigated in association with neutrophilic respiratory burst. To induce lung leak, we generated intestinal ischemia for 60 min prior to the 120 min reperfusion by clamping superior mesenteric artery in Sprague-Dawley rats. Acute lung leak was confirmed by the increased lung leak index and protein content in bronchoalveolar fluid. These changes were inhibited by mepacrine, the non-specific $PLA_2$ inhibitor. The lung myeloperoxidase (MPO) activity denoting the pulmonary recruitment of neutrophils was increased by intestinal I/R, but decreased by mepacrine. Simultaneously, the number of leukocytes in bronchoalveolar fluid was increased by intestinal ischemia/reperfusion (I/R) and decreased by mepacrine. Gamma glutamyl transferase activity, an index of oxidative stress in the lung, was increased after intestinal I/R but decreased by mepacrine, which implicates that $PLA_2$ increases oxidative stress caused by intestinal I/R. The $PLA_2$ activity was increased after intestinal I/R not only in the intestine but also in the lung. These changes were diminished by mepacrine. In the cytochemical electron microscopy to detect hydrogen peroxide, intestinal I/R increased the generation of the hydrogen peroxide in the lung as well as in the intestine. Expression of interleukin-1 (IL-1) in the lung was investigated through RT-PCR. The expression of IL-1 after intestinal I/R was enhanced, and again, the inhibition of $PLA_2$ suppressed the expression of IL-1 in the lung. Taken together, intestinal I/R seems to induce acute lung leak through the activation of $PLA_2$, the increase of IL-1 expression associated with increased oxidative stress by neutrophilic respiratory burst.

• Geophysics and Geophysical Exploration
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• v.25 no.3
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• pp.120-128
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• 2022

There are 17,000 reservoir dams in Korea, of which more than 85% were built over 50 years ago. Old embankment dams are weakened by internal erosion and suffusion phenomena due to preferential leakage paths and this ongoing weakening can cause their failure. Therefore, early warning associated with leakage in an embankment dam is crucial to prevent its failure. An electrical resistivity survey is a non-destructive, real-time and in-situ technique for detecting the development of leakage zones and general conditions of embankment dams. Because of its advantages, the electrical resistivity survey is widely used for reservoir safety inspections. However, the electrical resistivity survey is still not officially included in the precise safety inspection of reservoir dams because it cannot present a quantitative index of dam safety. In this study, we propose a method for calculating the leak index according to the water content evaluated from the electrical resistivity survey and/or induced polarization survey. Particularly, by proposing a quantitative leak index calculation method from monitoring surveys and independent surveys, we provide a theoretical basis for including electrical resistivity and induced polarization surveys as components of the precise safety inspection of reservoirs dams.

• The Korean Journal of Physiology and Pharmacology
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• v.1 no.1
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• pp.71-78
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• 1997

In an attempt to investigate the role of phospholipase $A_2$($PLA_2$) in interleukin-l (IL-l) induced acute lung injury, mepacrine was tried to inhibit $PLA_2$ in IL-l induced ARDS rats. For confirmation of acute lung injury by IL-l, and to know the role of neutrophils in this injury, lung leak index, lung myeloperoxidase(MPO), number of neutrophils and protein content in the bronchoalveolar lavage (BAL) and wet lung weight were measured. At the same time lung $PLA_2$ was measured to know the effect of IL-l on $PLA_2$ activity. Pulmonary surfactant was also measured for an investigation of type II alveolar cell function. Neutrophil adhesion assay was performed to know the effect of $PLA_2$ inhibition in vitro with human umbilical vein endothelial cells (HUVEC). For precise location of injury by IL-l, morpholgical study was performed by electron microscopy. Five hours after instillation of IL-l (50 ng/rat), lung leak index, protein content, number of neutrophils, lung MPO and wet lung weight were increased significantly. Five hours after IL-l instillation lung $PLA_2$ activity was increased significantly, and increased surfactant release was observed in IL-l induced ARDS rats' BAL. In contrast, in rats given mepacrine and IL-l, there was decrease of acute lung injury i.e. decrease of lung leak index, wet lung weight, protein content, number of neutrophils in BAL and decreased lung MPO activity. Mepacrine decreased surfactant release also. Interestingly, inhibition of $PLA_2$ decreased adhesion of human neutrophils to HUVEC in vitro. Morphologically, IL-l caused diffuse necrosis of endothelial cells, type I and II epithelial cells and increased the infiltration of neutrophils in the interstitium of the lung but after mepacrine treatment these pathological findings were lessened. On the basis of these experimental results it is suggested that $PLA_2$ has a major role in the pathogenesis of acute lung injury mediated by neutrophil dependent manner in IL-l induced acute lung injury.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.34 no.2
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• pp.137-143
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• 2010

Complete dryness inside the stator is a necessary and sufficient condition for the leak test. Microcracks caused by high cycle fatigue due to operation are generated in stator windings, and they are interrupted by water molecules during the leak test. For this reason, during leak test, the wrong value is indicated when there are no leaks in stator windings. Generator manufacturers presents unique dryness judgment criteria for the leak test, but there is no actual criterion that accurately indicates the dryness point for the leak test. The reason is because stator winding has a complex structure and the absence of effective dryness equipment in power plants. This paper proposes a dryness judgment criterion to evaluate if inside the stator winding is dried completely and presents experimental results.

• The Korean Journal of Physiology and Pharmacology
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• v.2 no.4
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• pp.479-491
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• 1998

In order to know the pathogenesis of adult respiratory distress syndrome (ARDS) in association with the oxidative stress by neutrophils, the role of platelet activating factor (1-0-alkyl-2-acetyl-snglycero-3-phosphocholine, PAF) was investigated during acute lung injury induced by interleukin- $1{\alpha}$ (IL-1) in rats. An insufflation of IL-1 into the rat's trachea increased the acetyltransferase activity in the lung and the increase of PAF content was followed. As evidences of acute lung injury by neutrophilic respiratory burst, lung leak index, myeloperoxidase activity, numbers of neutrophils in the bronchoalveolar lavage fluid, neutrophilic adhesions to endothelial cells and NBT positive neutrophils were increased after IL-1 treatment. In addition, a direct instillation of PAF into the trachea caused acute lung leak and the experimental results showed a similar pattern in comparison with IL-1 induced acute lung injury. For the confirmation of oxidative stress during acute lung leak by IL-1 and PAF, a histochemical electron microscopy was performed. In IL-1 and PAF treated lungs of rats, the deposits of cerrous perhydroxide were found. To elucidate the role of PAF, an intravenous injection of PAF receptor antagonist, WEB 2086 was given immediately after IL-1 or PAF treatment. WEB 2086 decreased the production of hydrogen peroxide and the acute lung leak. In ultrastructural study, WEB 2086 mitigated the pathological changes induced by IL-1 or PAF. The nuclear factor kappa B (NFkB) was activated by PAF and this activation was inhibited by WEB 2086 almost completely. Based on these experimental results, it is suggested that the PAF produced in response to IL-1 through the remodeling pathway has the major role for acute lung injury by neutrophilic respiratory burst. In an additional experiment, we can also come to conclude that the activation of the NFkB by PAF is thought to be the fundamental mechanism to initiate the oxidative stress by neutrophils causing release of proinflammatory cytokines and activation of phospholipase $A_2$.

• International journal of steel structures
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• v.18 no.5
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• pp.1710-1722
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• 2018

Investigating remained damages from terrible earthquakes, it could be concluded that some events including explosion because of defect and failure in the building mechanical facilities or caused by gas leak, firing, aftershocks, etc., which are occurred during or a few time after the earthquake, will increase the effects of damages. In this paper, by introducing a complete risk analysis which included direct and indirect risks for earthquake (the main shock) and aftershock, the corresponding robustness index was created that called as "robustness index sequential critical events risk-based". One of the main properties of the intended robustness index is using progressive collapse percentage in its evaluation. Then, in a numerical example for a 4-storey moment resisting steel frame structure, a method is presented for obtaining all effective parameters in robustness index evaluation based on the intended risk and at last its results were reported.

• Ocean Systems Engineering
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• v.8 no.1
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• pp.41-55
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• 2018

Accidental oil and gas leak is a critical concern for the offshore industry because it can lead to severe consequences and as a result, it is imperative to evaluate the probabilities of occurrence of the consequences of the leakage in order to assess the risk. Event Tree Analysis (ETA) is a technique to identify the consequences that can result from the occurrence of a hazardous event. The probability of occurrence of the consequences is evaluated by the ETA, based on the failure probabilities of the sequential events. Conventional ETA deals with events with crisp failure probabilities. In offshore applications, it is often difficult to arrive at a single probability measure due to lack of data or imprecision in data. In such a scenario, fuzzy set theory can be applied to handle imprecision and data uncertainty. This paper presents fuzzy ETA (FETA) methodology to compute the probability of the outcomes initiated due to oil/gas leak in an actual offshore-onshore installation. Post FETA, sensitivity analysis by Fuzzy Weighted Index (FWI) method is performed to find the event that has the maximum contribution to the severe sequences. It is found that events of 'ignition', spreading of fire to 'equipment' and 'other areas' are the highest contributors to the severe consequences, followed by failure of 'leak detection' and 'fire detection' and 'fire water not being effective'. It is also found that the frequency of severe consequences that are catastrophic in nature obtained by ETA is one order less than that obtained by FETA, thereby implying that in ETA, the uncertainty does not propagate through the event tree. The ranking of severe sequences based on their probability, however, are identical in both ETA and FETA.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.19 no.7
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• pp.537-544
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• 2007

To facilitate comparative analysis on the effects of numerous parameters concerning design and operation of a desiccant rotor, it is firstly required to represent the dehumidification performance as numerical indices. In this work is proposed two performance indices of a desiccant rotor: the humidity effectiveness and the enthalpy-leak ratio. The humidity effectiveness represents the actual dehumidification as compared with the dehumidification in an ideal case, while the enthalpy-leak ratio represents the enthalpy transfer from the regeneration side to the dehumidification side. In an ideal case, the two indices approach one and zero, respectively. The effects of numerous parameters on the dehumidification performance of a desiccant rotor are investigated through numerical simulation and represented with the two indices. The results show that the performance indices are mainly determined by three nondimensional parameters each representing the thermal capacity, the sorption capacity, and the transfer capacity of a desiccant rotor.

• Tuberculosis and Respiratory Diseases
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• v.44 no.6
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• pp.1343-1352
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• 1997

Background : Heat-treated cells are known to be protected from lysis by TNF, which is considered to play a central role in the pathogenesis of sepsis-induced acute lung injury. The objective of the study was to investigate the effect of heat shock response by heat-pretreatment on the acute lung injury of the rats induced by intratracheally administered TNF-$\alpha$, Methods : We intratracheally instilled either saline or TNF (R&D, 500ng) with and without heat pretreatment in Sprague-Dawley rats weighing 250~350 g. The heated rats were raised their rectal temperature to $41^{\circ}C$ and was maintained thereafter for 13 minutes at 18 h before intratracheal administration of saline or TNF. After 5 h of intratracheal treatment, lung leak, lung myeloperoxidase activity (MPO) and heat shock proteins were measured in rats. Lung leak index was defined as counts per minute of $I^{25}$ in the right lung divided by counts per minutes of $I^{25}$ in 1.0 ml of blood. All data are expressed as means ${\pm}$SE. Results : There is no difference in acute lung leak index ($0.099{\pm}0.024$ vs $0.123{\pm}0.005$) among the rats given saline intratracheally with and without heat pretreatment, but MPO activity showed a decreased tendency in heat-pretreated rats ($4.58{\pm}0.79\;U/g$) compared with heat-unpretreated rats ($7.32{\pm}0.97\;U/g$) (P=0.064). Rats administered TNF intratracheally with heat-pretreatment had decreased lung leak index ($0.137{\pm}0.012$) and lung MPO activity ($5.51{\pm}1.04\;U/g$) compared with those of heat-unpretreated and TNF-administered rats ($0.186{\pm}0.016$, $14.34{\pm}1.22\;U/g$) (P<0.05 in each). There were no significant difference of lung leak index and MPO activity between TNF-treated rats with heat-pretreatment and saline-treated rats with and without heat-pretreatment. Conclusion : The heat shock response attenuated neutrophil recruitment and acute lung leak induced by intratracheal instillation of TNF-in rats.