• Journal of fish pathology
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• v.19 no.2
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• pp.99-108
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• 2006

Diseased tiger puffer (Takijugu rubripes) showed anorexia and severe anemia in the gills. In wet mount preparations Heterobothrium sp. with 4 sets of clamps on the opisthohaptor attached to the gill filaments of the diseased fish. From the drum-shaped filter for filtration of the culturing water and the sediment of aquarium held the diseased fish in the laboratory, their eggs forming long strings through connection of the bipolar filaments could be easily collected. The parasites with 4 pairs of clamps on the opisthohaptor were 2.38mm in length and 0.71 mm in width, but had no isthmus which was separated the opisthohapor from the body proper. The parasites could be easily distinguished from Heterobothrium tetrodonis and H. okamotoi in the absence of distinct isthmus, and resembled H. yamagutii described from the gills of Takifugu xanthopterus in Japan. Their eggs were yellowish spindle in shape and 180-200 x 5-6 Iffil in size, which were pointed at both ends. The eggs were linked to adjacent egg shells like a string of beads by the filaments. Onchomiracidia, ciliated larvae spawned from the eggs were 133 Iffil (120-146 Iffil) x 751ffil (68-80 Iffil) in size. The length of the ciliates was 12 Iffil in the anterior part of the larvae and 14 Iffil in the posterior part. Round or leaf-shaped young parasites attached on the secondary gill lamellae. There was a tendency that most of round smaller parasites without an opisthohaptor were found in the middle part of the gill lamellae while leaf-shaped larger ones with a opisthohaptor attached on the distal part of the gill filaments. Each clamp of mature parasites grasped one secondary gill filament which resulted in bending of the filaments and hyperplasia of the epithelium and mucous cells. In the branchial cavity around the pseudobranch, mature parasites grasped the adjacent ones by means of the clamps, and the epithelia of the branchial cavity around the parasites showed severe irregular hyperplasia and erosion with strongly PAS-positive mucous cells.

• The Korea Journal of Herbology
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• v.26 no.3
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• pp.57-63
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• 2011

Objectives : Peucedani Radix ('Jeonho' in Korean) is the root of Peucedanum praeruptorum or Angelica decursiva. However, the root of Anthriscus sylvestris has usually been distributed as Jeonho. This study was performed to determine the discriminative criteria of Jeonho, focused on distribution in Korean markets. Methods : We have determined identification keys to discriminate each Jeonho samples, through observation of external morphology of original plants, and the research of external and internal morphological features of dried Jeonho herbs. Results : Because of identical to the Korean plant name 'Jeonho', Anthrisci Radix had been came into use as a substitute of Jeonho in Korea during the time of Japanese colonial rule. The original plants of Jeonho and its substitute are discriminated with shape of leaf, leaf margin and color of stem. External morphological features of the medicinal herbs of Jeonho are different in the color of cross-sections, pellucid dot, white powder. Internal morphological points, such as fiber bundle of xylem, seconadary mudullary ray and ray of xylem were also used as discriminative criteria for Jeonho. Further details(e.g. identificaion keys) are in the article. Conclusions : We think that these discriminative criteria will be meaningful in identifying the substitutes and adulterants of Jeonho.

• Korean Journal of Plant Resources
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• v.25 no.2
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• pp.257-270
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• 2012

The taxonomic relationship of 3 taxa in $Carex$ sect. $Siderostictae$ were revealed by morphological and molecular studies. The morphological characteristics of Korean $Carex$ siderosticta Hance, which is a type species of sect. $Siderostictae$, was first reviewed in comparison with previous records in order to identify the difference. Then the distribution map of this species was formulated through previous floral literatures and voucher specimens. Although the leaf shape which is represented by length/width ratio showed a great variation depending on habitat condition, the rhizome creeping patterns, the presence of hair on the surface leaf margin and perigynium were shown to be good diagnostic characters to define delimitation of species level. The results from molecular study supported $C.$ $ciliatomarginata$ Nakai should be ranked independently on species level.

• Korean Journal of Plant Taxonomy
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• v.42 no.2
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• pp.178-183
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• 2012

We report a newly naturalized species of the genus Bidens L. (Asteraceae) in Korea, specifically Bidens subalternans DC. var. subalternans. This naturalized plant was discovered Geumjung-gu in Busan; Gyeongsan-si, Jillyang-eup in Gyeongbuk-do; and in Buk-gu in Daegu. Bidens subalternans var. subalternans is distinguishable from other Bidens in Korea by the following combination of characteristics: an odd-pinnate leaf, deeply lobed, narrow lanceolate, four lobes in the corolla, an oblong achene shape. This taxon is termed 'Wang-do-kka-bi-ba-neul' as a common Korean name based on its large size (in particular, the size of leaf). The key for all taxa of the genus Bidens in Korea is also provided here.

• Korean Journal of Plant Taxonomy
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• v.35 no.2
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• pp.115-127
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• 2005

Two anatomical features of leaves, transverse section of midribs and petioles, were examined for nine species of five genera of Thymelaeaceae, Daphne L. -4 spp., Diarthron Turcz. -1 sp., Edgeworthia Meisn. -1 sp., Stellera L. -1 sp., Wikstroemia Endl. -2 sp., using light microscopy and scanning electron microscopy. In terms of shape of upper side, three types were observed, i.e., flattened, depressed, and raised. Outline of petioles is mostly semi-circular or circular in transverse section, with two distinct wings/weakly developed wings or without wings. Other characters besides anatomical features of leaves such as vascular bundle tissues, crystal, tannin are also described in details. Finally, we briefly discussed the systematic significance of the anatomical features of leaves for identification of Korean Thymelaeaceae.

• Korean Journal of Plant Resources
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• v.28 no.6
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• pp.697-703
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• 2015

Twenty apple germplasm accessions from the Korean Genebank were successfully cryopreserved using two-step freezing to back up genetic resources maintained by field collections. This study examined the morphological and genetic stability of cryopreserved dormant apple buds that were stored in liquid nitrogen, and then rewarmed and regrown. Whole plants were regenerated directly from dormant buds through budding without an intermediary callus phase. The cryopreserved buds produced high levels of shoot formation (76.2-100%), similar to those of noncryopreserved buds (91.3-100%), with no observed differences between cryopreserved and noncryopreserved materials. Three of the twenty cryopreserved apple germplasm accessions were used to assess morphological and genetic stability. No differences in morphological characteristics including shoot length, leaf shape, leaf width/length ratio, and root length were observed between controls (fresh control and noncryopreserved) and cryopreserved plantlets. The genetic stability of regenerants (before and after cryopreservation) was investigated using inter simple sequence repeat (ISSR) markers. The ISSR markers produced 253 bands using four primers, ISSR 810, SSR 835, ISSR 864, and ISSR 899. These markers showed monomorphic banding patterns and revealed no polymorphism between the mother plant and regenerants before and after cryopreservation, suggesting that cryopreservation using two-step freezing does not affect the genetic stability of apple germplasm. These results show that two-step freezing cryopreservation is a practical method for long-term storage of apple germplasms.

• Korean Journal of Agricultural Science
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• v.2 no.1
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• pp.143-149
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• 1975

To obtain some basic informations on the breeding of Kudzu, 42 individuals were selected and their morphological characteristics were investigated and analyzed. The shape of leaves was classified as 4 types and area of leaves into 5 steps. Internode length was distributed between 13 cm and 37 cm. Significant correlations were recognized between petiole and leaf area, between internode length and internode diameter and between internods diameter and leaf area.

• Nutritional Sciences
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• v.3 no.1
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• pp.3-10
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• 2000

The present study was aimed at investigating the nutritional and physiological significance of rice bran as a source of dietary fiber as compared to pectin and wheat bran. The parameters for comparison included hypertrophy and morphology of intestines, stool weights and villus marker enzyme activity. For 6 weeks, 10 Sprague Dawley male rats were given one of six experimental diets: 1% cellulose control (CC), 5% pectin (P5), 5% rice bran(RB5), 10% rice bran(RB10), 5% wheat bran (WB5) or 10% wheat bran (WB10) based on the level of dietary fiber. Among experimental groups, food efficiency ratio and body weight gain was comparable. RB10 increased cecal and colonic tissue weights and content weights of cecum and colon as much as P5 did. Stool weight was positiviely correlated with colonic tissue weight (r=0.727, P<0.001), with colonic content weight(r=0.647, P<0.001). Small intestine length increased most in the P5 group, followed by the RB10 group. The scanning electron micrograph of jejunal villi from rice bran groups showed a leaf-shaped, smooth and regular pattern, whereas that of CC group produced a rather long shape. The wheat bran groups showed an irregular leafshaped pattern, and the pectin group typically produced leaf-shaped villi with surface damage. The activities of villus marker enzymes (maltase and sucrase) were higher in the bran-fed rats than in the control or pectin-fed rats. The results indicate than not only dietary fiber amounts but also fiber sources are closely related to the physiology and morphology of the large and small intestines in rats. Rice bran exerted effects on fecal output and trophic effects on the intestines similar to those of pectin.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.587-590
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• 2009

New onion hybrid cultivar, "Yeongpunghwang" was developed from cross between "MOS8", male sterile line and "Mokpo 11", pollen line by Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA in 2008. The crossing line was performed yield test in 2003. IT was evaluated as "Mokpo 11" in the regional yield trial test(RYT) from 2006 to 2008 during three years. This line is semi-erect plant growth type and has green leaf colour. It has broad ovate bulb shape and yellow skin colour in bulb characteristics. Plant height, leaf number, and bolting rate was 78cm, 7.9, and 3.9%, respectively. Lodging date as harvesting indicator was on 26 May. The yield potential of "Yeongpunghwang" was out-yielding 6% compare to check variety ("Sunpower") by with 8,344 kg/10a in RYT. It showed moderated cold hardness in all regional location which was conducting RYT, including Muan, Goheung, Changyeong and Andong. So this variety was recommend as a leading variety at southern area of korea.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.291-294
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• 2008

Cymbidium 'Honey Girl' was developed in 2005 by the National Horticultural Research Institute, Rural Development Administration. This cultivar came from cross between Cym. 'Yakoto' and Cym. 'Marian Simplicity' in 1995. Fifty seven seedlings were obtained after planting and acclimatization in green house. In 1999, a line was selected by its performance such as flower color, leaf shape, flower stalk, and vigorous growth which was named 'Wongyo F1-13'. The line code '9521928'($V_2$) had uniformity and excellent characteristics. The selected line was named as 'Honey Girl' after second characteristics test. This cultivar had white color ('WN 155B') and medium sized leaves and flowers (average leaf length is about 57 cm and width of flower is about 7.7cm) with many flowers and erect flower stalks. Blooming begins from the end of November to December under the normal culture conditions.