• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.3
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• pp.171-176
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• 2006

This experiment was carried out to breed the cold-tolerant variety of Italian ryegrass(Lolium multiflorum Lam.) in Grassland and Forages Division, National Livestock Research Institute, RDA, Suwon from 1987 to 2005. New variety, Kowinner as a tetraploid variety was dark green in leaf color and growth habit in autumn and early spring was semi-prostrate and medium, respectively Kowinner as a medium or late-heading variety was 19th May in heading date. Flag leaf length of Kowinner was 29 cm that was the same as control variety, Hwasan 101. Length of longest stem and plant length of Kowinner were 70 cm and 97 cm that was longer 3 cm than those of Hwasan 101, respectively. Stem width of Kowinner was thicker than that of Hwasan 101. Expecially, Kowinner was very good in regrowth. Cold tolerance of Kowinner was slightly weaker than that of Hwasan 101 and slightly stronger than that of Florida 80. Dry matter(DM) yield(12,884 kg/ha) of Kowinner was 8% more than that of Hwasan 101. In vitro dry matter digestibility and total digestible nutrient(TDN) of Kowinner were 76.2 and 63.3% that was 1.6 and 0.4% lower than those of Hwasan 101, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.275-280
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• 2005

This experiment was carried out to breed the cold-tolerant variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon from 1997 to 2002. Hwasan 104 as a tetraploid variety was dark green in leaf color and had semi-prostrate and medium growth habit in late autumn and early spring, respectively. Hwasan 104 was 19th May in heading date as a late-heading variety. Especially, Hwasan 104 was broader in flag leaf and longer 7 cm in plant length, and thicker in stem width than those of control variety, Hwasan 101. Cold tolerance of Hwasan 104 was better than that of Hwasan 101 registered as a cold-tolerant variety. Dry matter (DM) yield of Hwasan 104 was $5\%$ more than DM 9,348 kg/ha of Hwasan 101. In vitro dry matter digestibility and total digestible nutrient of Hwasan 104 were 74.3 and $63.1\%$ which are 3.3 and $1.5\%$ lower than those of Hwasan 101, respectively. Acid detergent fiber and neutral detergent fiber of Hwasan 104 were 32.6 and $55.8\%$ which are 1.9 and $2.4\%$ higher than those of Hwasan 101, respectively.

• Korean Journal of Community Nutrition
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• v.15 no.4
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• pp.525-535
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• 2010

The purpose of this study was to provide basic data for nutritional education for juveniles' desirable vegetable intake by analyzing their vegetable intake, dietary habit and nutrient intakes according to gender of middle school students in Chungnam. The average age of the subjects was 15.0, height was 162.5 cm, weight was 53.5 kg and their average BMI was $20.1kg/m^2$. The frequency of eating supper in girls was lower than that in boys. Many respondents answered that a meal-time was 10~20 minutes. Without gender difference, more than 90% respondents thought that vegetables were good for health. As for the preference of vegetable, subjects responded, "I am in the middle", "I like them", "I dislike them", "I like them very much", and "I dislike them very much" in order, without gender difference. The frequent eaten leaf vegetable was Chinese cabbage, the frequent eaten fruit vegetable was cucumber, and the frequent eaten root vegetable was radish. The favorite leaf vegetable was lettuce, the favorite fruit vegetable was corn, and the favorite root vegetable was sweet potato. The preference degrees of taro and ginger were very low. The reasons why they liked a vegetable were that it was delicious and they ate it at home often. In addition, the reason why they disliked vegetables was that they are untasty in flavor or texture and it showed that many students had a prejudice that vegetables were untasty. The intakes of plant protein, dietary fiber, ash and INQs of dietary fiber, calcium, vitamin C, folate, vitamin E in the group with high preference of vegetables were significantly higher than those of low preference group. The study results indicate that intake frequency and preference of root vegetables in juveniles are low and the major reasons of these results are taste and eating experience of vegetables. In addition, the intake amounts of dietary fiber and folate are poor in the subjects with low preference of vegetables. Therefore, families and schools should make efforts that juveniles can recognize the importance of vegetable intake and select various vegetables properly through the development of cooking methods and systematic nutrition education.

• Korean Journal of Plant Taxonomy
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• v.43 no.1
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• pp.12-21
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• 2013

To clarify the taxonomic position for Astragalus nakaianus and provide correct scientific name for A. mongholicus cultivar in South Korea, we examined external morphological characters and sequence variations from ITS and five cp non-coding DNA regions. Genetic structure was also analyzed for 61 individuals from three populations using nine microsatellite loci. We found no significant difference between the South Korean cultivar and A. mongholicus var. dahuricus when morphology and ITS sequences were considered. Morphologically, A. nakaianus specimens varied somewhat from A. mongholicus var. mongholicus and var. dahuricus in habit, plant height, and lengths of leaf axis and leaflet. Although sequence data from ITS and cp noncoding DNA regions could not distinguished A. nakaianus from A. mongholicus, microsatellite analysis revealed strong structuring between the cultivar and A. nakaianus. Therefore, we conclude that the South Korean A. mongholicus cultivar should be treated as A. mongholicus var. dahuricus and that A. nakaianus should be merged into A. mongholicus as a variety, i.e., A. mongholicus var. nakaianus.

• Journal of Plant Biotechnology
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• v.3 no.2
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• pp.89-94
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• 2001

In vitro plant regeneration of inbred breeding line of hot pepper (Capsicum annuum L.) was established using leaf and petiole segments as explants. About 28 days old plants were excised and cultured on MS medium supplemented with TDZ and NAA or in combination with Zeatin. In all of the media compositions tested, combination of TDZ 0.5 mg/L, Zeatin 0.5 mg/L, and NAA 0.1 mg/L was found to be the best medium for shoot bud initiation. Young petiole was the most appropriate explant type for the plant regeneration as well as genetic transformation in hot pepper. In this study, HpMADS1 gene isolated from hot pepper was introduced using Agrobacterium-mediated transformation system. Based on the analysis of Southern blot and RT-PCR, HpMADS1 gene was integrated in the hot pepper genome. It has been known that floral organ development is controlled by a group of regulatory factors containing the MADS domain. Morphological characteristics in these transgenic plants, especially flowering habit, however, were not significantly altered, indicating this MADS gene, HpMADS1 may be non-functional in this case.

• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.249-256
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• 2007

A study was conducted to observe the variation and inheritance of agronomic traits and their interrelationship in mungbean. The objective of the study was to compare agronomic traits and hardseed percentage of 268 recombinant inbred lines(RILs) developed from the cross between wild Vigna subspecies sublobata "ACC 41" with the mungbean cultivar "Berken". The RIL population and their parents were evaluated under controlled conditions in a glass house at the University of Queensland, Brisbane, Australia. The results showed significant differences among the RILs and among the parents in all traits under study. Berken had a longer flowering date and a higher seed weight per plant, but less total leaf number and pod number per plant than ACC 41. A germination test between papers revealed that ACC 41 was 100% hard-seeded and did not germinate at all, while Berken germinated up to 100%. Their RILs distributed well between 0 to 100% hardseed. Upon scarification, all hardseed germinated within seven days. Narrowsense heritability estimates of total leave number, hardseedness, pod length, and pod width were highly heritable at 89.9, 98.9, 93.7, and 93.2%, respectively. The heritability of seed weight per plant and number of seeds per plant were lower at 63.1 and 58.4%, respectively. Seed weight per plant showed positive transgressive segregation when compared with ACC 41 and a positive correlation with 100 seed weight. While the number of seeds per pod showed a negative transgressive segregation when compared with Berken and a negative correlation with pod length and pod width. The RILs gave a 1:1 segregation ratio in leaflet shape, growth habit, and growth pattern, indicating that these traits were controlled by a single dominant gene.

• Journal of Plant Biology
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• v.37 no.1
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• pp.43-51
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• 1994

Spore morphology of 14 species of Ophioglossaceae is examined, and descriptions and key based on spore morphology are provided. In addition, spores of one species of Angiopteris and three species of Osmunda are examined for comparision with those of Ophioglossaceae. Spore shape at proximal face is sub triangular or triquete in Botrychium and circular in Ophiog~ lossum, Angiopteris and Osmunda. Based on the wall sculpturing patterns, three groups can be recognized within Botrychium, and the differences of wall sculpturing among these groups are smaller as compared to those among Botrychium, Ophioglossum, Osmunda and Angiopteris. This result supports the previous infrafamilial system of Clausen, which was mainly based on the characteristics of vernation, habit, fertile and sterile segments of the leaf, and gametophyte. Spores of nine species of Ophioglossum, which are circular in proximal face and fossulate or foveolate in wall sculpturing, are quite uniform as compared to those of Botrychium. Therefore they should be kept under a single genus rather than split into four genera suggested by Nishida (1952).(1952).

• Korean Journal of Breeding Science
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• v.41 no.1
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• pp.36-39
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• 2009

A new mungbean cultivar "Dahyeon" (Vigna radiata (L.) Wilczek) was derived from the cross between Jeonnam11 and AV-1-3-34-204 at National Institute Crop Science (NICS) and Jeollanamdo Agricultural Research & Extension Services (JARES) in 2006. "ahyeon" has erect growth habit, heart leaflet, green hypocotyl, black and straight pod when matured, and dull green seed surface. The pods number per plant was 21.0, which was 4.0 more than check cultivar "wool" The plant height and thousand seed weight were 57cm and 48 g respectively. The sprout yield was up to 5% more than that of the check cultivar. It was field resistance to mungbean mottle virus, cercospora leaf spot, and powdery mildew. The average yield was 1.90 ton/ha, which was 17% more than the check cultivar.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.543-546
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• 2009

Intermediate parent "Wonkyo Ga-Dangwagi 1" (Malus domestica Borkh.) was released from Fruit Research Division, National Institute of Horticultural & Herbal Science, Korea in 2008. It was derived from the cross between "Starkrimson" and columnar type "McIntosh Wijcik" in 1992 after selection tests from 2000 to 2008. Trees are moderately vigorous and have spreading branching habit. It bears abundant flower buds on one-year-old branches and fruits mainly on spurs or short branches. Harvesting time of this variety is late September, 7 days later than that of "Hongro" in Suwon. The fruit is conical to narrow conical in shape and bright red in skin color. Fruit size is small with weight of 200~240g on an average and fruits have $14^{\circ}Bx$ soluble solid and medium acidity. It is moderately susceptible to alternaria leaf spot (Alternaria alternata). "Wonkyo Ga-Dangwagi 1" is a promising apple cultivar and will appeal to young people with good sugar-acid balance.

• Korean Journal of Breeding Science
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• v.43 no.3
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• pp.218-221
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• 2011

A mungbean cultivar 'Juhyeon' (Vigna radiata (L.) Wilczek) was derived from the cross between Keumseong and VC1834-4-B-2-B-3B-B at Jeollanamdo Agricultural Research & Extension Services (JARES) in 2009. 'Juhyeon' has an erect growth habit, heart leaflet, green hypocotyl, black and straight pod when matured, and dull green seed surface. The pod number per plant was 26, which was 8 more than check cultivar 'Owool'. The plant height and thousand seed weight were 66 cm and 47 g, respectively. This cultivar has field resistance to mungbean mottle virus, cercospora leaf spot, powdery mildew, and lodging. The sprout yield was up to 18% more than that of the check cultivar. The average yield was 1.84 MT/ha, which was 6% more than the check cultivar.