Browse > Article
http://dx.doi.org/10.11110/kjpt.2013.43.1.12

Taxonomic position and genetic differentiation of Korean Astragalus mongholicus Bunge  

Choi, In-Su (Department of Biological Sciences, Inha University)
Kim, So-Young (Nature Conservation Research Division, National Institute of Environmental Research)
Choi, Byoung-Hee (Department of Biological Sciences, Inha University)
Publication Information
Korean Journal of Plant Taxonomy / v.43, no.1, 2013 , pp. 12-21 More about this Journal
Abstract
To clarify the taxonomic position for Astragalus nakaianus and provide correct scientific name for A. mongholicus cultivar in South Korea, we examined external morphological characters and sequence variations from ITS and five cp non-coding DNA regions. Genetic structure was also analyzed for 61 individuals from three populations using nine microsatellite loci. We found no significant difference between the South Korean cultivar and A. mongholicus var. dahuricus when morphology and ITS sequences were considered. Morphologically, A. nakaianus specimens varied somewhat from A. mongholicus var. mongholicus and var. dahuricus in habit, plant height, and lengths of leaf axis and leaflet. Although sequence data from ITS and cp noncoding DNA regions could not distinguished A. nakaianus from A. mongholicus, microsatellite analysis revealed strong structuring between the cultivar and A. nakaianus. Therefore, we conclude that the South Korean A. mongholicus cultivar should be treated as A. mongholicus var. dahuricus and that A. nakaianus should be merged into A. mongholicus as a variety, i.e., A. mongholicus var. nakaianus.
Keywords
Taxonomic position; Astragalus mongholicus cultivar; Astragalus nakaianus; external morphological characters; genetic differentiation;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Weising, K. N., H. Nybom, H. Wolff and G. Kahl. 2005. DNA Fingerprinting in Plants: Principles, Methods and Applications. Taylor & Francis, Boca Raton. Pp. 41-45.
2 White, T. J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A guide to methods and applications. Innis, M. A., D. H. Gelfaud, J. J. Sninsky and T. J. White (eds.), Academic Press, San Diego, Califonia. Pp. 315-322.
3 Xu, L. R. and D. Podlech. 2010. Astragalus L. In Flora of China, Vol. 10. Wu, Z.Y., P. H. Raven and D. Y. Hong (eds.), Science Press, Beijing. Pp. 328-453.
4 Yang, J. Y., H. K. Kim and S. J. Park. 2011. Development of genetic marker specific for Korean Hwanggi medicine (Radix Astragali). Food Science and Biotechnology 20: 1561-1567.   DOI
5 Yao, X. H., Q. Ye, P. W. Fritsch, B. C. Crux and H. Huang. 2008. Phylogeny of Sinojackia (Styracaceae) based on DNA sequence and microsatellite data: implications for taxonomy and conservation. Annals of Botany 101: 651-659.   DOI   ScienceOn
6 Yip, P. Y. and H. S. Kwan. 2006. Molecular identification of Astragalus membranaceus at the species and locality levels. Journal of Ethnopharmacology 106: 222-229.   DOI   ScienceOn
7 Zhao, Y. Z. 2006. Taxonomy and floristic geographical distribution of the Chinese medicinal Huangqi. Bulletin of Botanical Research 26: 532-538. (in Chinese)
8 Zhu, X. Y. 1996. An additional note on karyotype analysis of Astragalus penduliflorus Lam. complex (Fabaceae). Guihania 16: 61-63. (in Chinese)
9 Zhu, X. Y. 2005. Revision of the Astragalus penduliflorus complex (Leguminosae-Papilionoidae). Nordic Journal of Botany 23: 283-294.
10 Zhu, X. Y. and C. J. Chen. 1991. Taxonomic significance of peptides from seed proteins of Astragalus penduliflorus complex (Fabaceae) and related taxa. Cathaya 3: 65-72.
11 Earl, D. A. and B. M. von Holdt. 2012. STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources 4: 359-361.   DOI   ScienceOn
12 Baldwin, B. G. 1992. Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: an example from the Compositae. Molecular Phylogenetics and Evolution 1: 3-16.   DOI   ScienceOn
13 Baldwin, B. G., M. J. Sanderson, J. M. Porter, M. F. Wojciechowski, C. S. Campbell and M. J. Donoghue. 1995. The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Annals of the Missouri Botanical Garden 82: 247-277.   DOI   ScienceOn
14 Bowles, V. G., R. Mayerhofer, C. Davis, A. G. Good and J. C. Hall. 2010. A phylogenetic investigation of Carthamus combining sequence and microsatellite data. Plant Systematics and Evolution 287: 85-97.   DOI
15 Bunge, A. 1868. Generis Astragali species Gerontogeae. Memoires de l'Academie Imperiale des Sciences de Saint Petersbourg, Septieme Serie 11: 1-140.
16 Chaudhary, L. B., T. S. Rana and K. K. Anand. 2008. Current status of the systematics of Astragalus L. (Fabaceae) with special reference to the Himalayan species in India. Taiwania 53: 338-355.
17 Chen, C. J. and X. Y. Zhu. 1990. Karyotype of Astragalus penduliflorus Lam. complex (Leguminosae) and its cytotaxonomic significance. Cathaya 2: 139-150.
18 Choi, I. S. and B. H. Choi. Isolation and characterization of 10 microsatellite loci from Korean Astragalus mongholicus (Fabaceae). Journal of Genetics. In press.
19 Dong, T. T. X., X. Q. Ma, C. Clarke, Z. H. Song, Z. N. Ji, C. K. Lo and K. W. R. Tsim. 2003. Phylogeny of Astragalus in China: molecular evidence from the DNA sequences of 5S rRNA spacer, ITS, and 18S rRNA. Journal of Agricultural and Food Chemistry 51: 6709-6714.   DOI   ScienceOn
20 Evanno, G., S. Regnaut and J. Goudet. 2005. Detecting the number of clusters of individuals using the software structure: a simulation study. Molecular Ecology 14: 2611-2620.   DOI   ScienceOn
21 Fu, K. T., Y. C. Ho. and S. B. Ho. 1993. Astragalus. In Flora Reipublicae Popularis Sinicae, Vol. 42 (1). Fu, K. T. (eds.), Science Press, Beijing. Pp. 78-349. (in Chinese)
22 Guo, H. Y., W. W. Wang, N. Yang, B. L. Guo, S. Zhang, R. J. Yang, Y. Yuan, J. L. Yu, S. N. Hu, Q. S. Sun and J. Yu. 2010. DNA barcoding provides distinction between Radix Astragali and its adulterants. Science China Life Sciences 53: 992-999.   DOI   ScienceOn
23 Hancock, J. M. 1999. Microsatellites and other simple sequences: genomic context and mutational mechanisms. In Microsatellites: Evolution and Applications. Goldstein, D. B. and C. Schlotterer (eds.), Oxford University Press, New York. Pp. 1-9.
24 Im, R. J. 1998. Flora Coreana, Vol. 4. The Science and Technology Publishing House, Pyongyang. Pp. 123-124. (in Korean)
25 Kazempour Osaloo, S., A. A. Maassoumi and N. Murakami. 2003. Molecular systematics of the genus Astragalus L. (Fabaceae): phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacers and chloroplast gene ndhF sequences. Plant Systematics and Evolution 242: 1-32.   DOI
26 Kim, S. Y., H. W. Choi, C. S. Kim, J. S. Sung, J. Lee and J. W. Bang. 2006. Cytogenetic analyses of Astragalus species. Korean Journal of Medicinal Crop Science 14: 250-254. (in Korean)   과학기술학회마을
27 Kim, Y. G., S. Y. Son, N. S. Seong and B. H. Lee. 2000. Flowering habit, pollination patterns and seed setting in Astragalus membranaceus Bunge. Korean Journal of Crop Science 45: 171-175. (in Korean)   과학기술학회마을
28 Korea National Arboretum. 2008. Rare Plants Data Book in Korea. Geobook, Seoul. Pp. 110. (in Korean)
29 Lee, T. B. 2003. Coloured Flora of Korea, Vol. 1. Hyang Mun Sa, Seoul. Pp. 636. (in Korean)
30 Lee, Y. N. 1981. New taxa of Korean flora: 3. Korean Journal of Botany 24: 27-30.   과학기술학회마을
31 Lock, J. M. and K. Simpson. 1991. Legumes of West Asia, a Check List. Royal Botanical Gardens, Kew.
32 Lee, Y. N. 2006. New Flora of Korea, Vol. 2. Gyohaksa, Seoul. Pp. 738. (in Korean)
33 Lee, Y. N. and C. A. Tho. 1959. Comparative study of Astragalus membranaceus Bunge and Astragalus hallasanenesis. Journal of Korean Culture Research Institute 1: 359-366. (in Korean)
34 Litt, M. and J. A. Luty. 1989. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. American Journal of Human Genetics 44: 388-396.
35 McNeill, J., F. R. Barrie, W. R. Buck, V. Demoulin, W. Greuter, D. L. Hawksworth, P. S. Herendeen, S. Knapp, K. Marhold, J. Prado, W. F. Prud'homme van Reine, G. F. Smith, J. H. Wiersema and N. J. Turland. 2012. International Code of Nomenclature for Algae, Fungi, and Plants (Melbourne Code) adopted by the Eighteenth International Botanical Congress, Melbourne, Australia, 2011. Regnum Vegetabile vol. 154. Koeltz Scientific Books, Konigstein.
36 Moench, C. 1794. Methodus Plantas Horti Botanici et Agri Marburgensis. Marburgi Cattorum. Pp. 167.
37 Mort, M. E., J. K. Archibald, C. P. Randle, N. D. Levsen, T. R. O'Leary, K. Topalov, C. M. Wiegand and D. J. Crawford. 2007. Inferring phylogeny at low taxonomic levels: utility of rapidly evolving cpDNA and nuclear ITS loci. American Journal of Botany 94:173-183.   DOI   ScienceOn
38 Nakai, T. 1914. Flora of Quelpeart and Wangto Island. Government General of Chosen, Seoul. Pp. 55 (in Japanese)
39 Nishizawa, T. and Y. Watano. 2000. Primer pairs suitable for PCRSSCP analysis of chloroplast DNA in angiosperms. Journal of Phytogeography and Taxonomy 48: 63-66.
40 Ohashi, H. 2001. Leguminosae. In Flora of Japan, Vol. 2b. Iwatsuki, K., D. E. Boufford and H. Ohba (eds.), Kodansha, Tokyo. Pp. 213-279.
41 Sang, T., D. J. Crawford and T. F. Stuessy. 1997. Chloroplast DNA phylogeny, reticulate evolution and biogeography of Paeonia (Paeoniaceae). American Journal of Botany 84: 1120-1136.   DOI   ScienceOn
42 Peakall, R. and P. Smouse. 2006. GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288-295.   DOI   ScienceOn
43 Podlech, D. 1999. New Astragali and Oxytropis from North Africa and Asia, including some new combinations and remarks on some species. Sendtnera 6: 135-174.
44 Pritchard, J. K., M. Stephens and P. Donnelly. 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945-959.
45 Shaw, J., E. Lickey, J. T. Beck, S. B. Farmer, W. Liu, J. Miller, K. C. Siripun, C. T. Winder, E. E. Schilling and R. L. Small. 2005. The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. American Journal of Botany 92: 142-166.   DOI   ScienceOn
46 Shishkin, B. K. 1946. Leguminosae: Astragalus L. In Flora of the U.S.S.R., Vol. 12. Komarov, V. K. (eds.), Academy of Science of the U.S.S.R., Moskva-Leningrad. Pp. 1-681.
47 Taberlet, P., L. Gielly, G. Patou and J. Bouvet. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology 17: 1105-1109.   DOI   ScienceOn
48 Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin and D. G. Higguns. 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24: 4876-4882.
49 Toh, C. A. 1971. A cytotaxonomic study on the Astragalus membranaceus and Astragalus membranaceus var. alpinus. Korean Journal of Plant Taxonomy 3: 57-61. (in Korean)