• Title/Summary/Keyword: leaf development

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The Extrapolations to Reduce the Need for Pesticide Residues Trials on Continuous Harvesting Leafy Vegetables (농약 잔류 시험을 위한 연속수확 엽채소류의 외삽)

  • Son, Kyeong-Ae;Im, Geon-Jae;Hong, Su-Myeong;Kim, Chan Sub;Gil, Geun-Hwan;Jin, Yong-Duk;Kim, Jinba;Ihm, Yang Bin;Ko, Hyeon Seok;Kim, Jang Eok
    • The Korean Journal of Pesticide Science
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    • v.17 no.4
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    • pp.293-301
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    • 2013
  • This study was carried out to investigate the pesticide residue pattern among different leafy vegetables applied with foliar spraying under greenhouse and to check extrapolating from some residue trial data to other minor crops. Leafy vegetables used in this study were: Mustard greens (Brassica juncea L.), Kale (Brassica oleracea L.), Dacheongchae (a kind of pak-choi (Brassica rapa subsp. chinensis L.)), Leaf broccoli (Brassica oleracea var alboglabra), Perilla leaf (Perilla frutescens (L.) Britton var. Frutescens), Leaf lettuce (Lactuca sativa L.), Swiss chard (Beta vulgaris L. subsp. vulgaris) and Red leaf chicory (Cichorium intybus L. var. foliosum Hegi). These are cultivated all year under indoor or outdoor and cut the leaf from plant continuously during harvest time. The amounts of pesticide deposit in/on the continuous harvesting leafy vegetables were affected by the ratios of leaf area to weight. Ratio of perilla leaf was the largest among crops as 58 $cm^2/g$. The residue levels of 7 pesticides in/on perilla leaf were the highest than those of other crops through the statistical analysis from zero day to fifth day after last application. The representative crop in 8 crops was perilla leaf selected based on the amounts of daily consumption and the high residues. This study suggest that the continuous harvesting leafy greens should be separated from the one time harvesting leafy vegetables for the pesticide recommendations because of different harvesting habits and pre-harvest intervals.

Regulation of Leaf Polarity during Leaf Development (잎의 발생과정에 있어서의 극성제어)

  • Cho, Kiu-Hyung;Jun, Sang-Eun;Tsukaya , Hirokazu;Kim, Gyung-Tae
    • Korean Journal of Plant Taxonomy
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    • v.38 no.1
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    • pp.51-61
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    • 2008
  • Leaves are indeterminate organs and possess a lot of genes which is involved in establishing leaf polarities. These polarities are regulated relatively early during leaf development and defined relative to the factors intrinsic to the primordia and interactions with the shoot apical meristem (SAM). Recently, several genes that control the polarity of lateral organs have been identified. Our genetic study of deformed root and leaf1 (drl1) mutant, which produces narrow, filament‐like leaves and defective meristems, revealed that DRL1 is involved in the regulation of SAM activity and leaf polarity. The DRL1 gene was found to encode a novel protein showing homology to Elongator‐associate protein (EAP) of yeast KTI12. The amino acid sequence of DRL1 is universally conserved in prokaryotes and eukaryotes. DRL1 and the plant DRL1 homologs clearly formed a monophyletic clade, suggesting the evolutionary conservation of DRL1 homologs was maintained in the genomes of all land plants.

Cadmium and zinc interaction and phytoremediation potential of seven Salix caprea clones

  • Han, Sim-Hee;Kim, Du-Hyun;Lee, Jae-Cheon
    • Journal of Ecology and Environment
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    • v.33 no.3
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    • pp.245-251
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    • 2010
  • We evaluated the interaction between Cd and Zn in the bioaccumulation of seven clones of Salix caprea, which were exposed both to Cd and Zn alone and to a combination of Cd and Zn. Cadmium (Cd) and Zn concentration in the four treatments were administered in the following order: root > leaf > stem, and obvious differences were noted among the treatments and clones. The leaf Cd concentration of clone BH2 and stem Cd concentration of clone BH5 in the combined Cd and Zn treatment group was increased by 62% and 110%, respectively, relative of that of the Cd alone treatment group. On the other hand, the leaf and stem Zn concentration of clone BH8 in the combined Cd and Zn treatment group was reduced by 66% and 61%, respectively, relative to that of the Zn alone treatment group. Translocation of Cd and Zn from the root was higher in the leaf than in the stem, and the combined Cd and Zn treatment stimulated the translocation of Cd from the root to the leaf and stem, whereas it suppressed the translocation of Zn from the root to the leaf and stem. Therefore, the interaction effects were considered strongly synergistic with Cd in the presence of Zn, but proved antagonistic to Zn in the presence of Cd in the combined Cd and Zn treatment group. The phytoremediation potentials of the seven clones, which were estimated from standard indices of Cd and Zn concentration in Cd and Zn alone and the combined Cd and Zn treatment groups, were highest in clone BH3, and lowest in clone BH5. Therefore, we recognize S. caprea as an appropriate material for phytoremediation, and this is particularly the case with clone BH3. However, further research will be required to evaluate the effects of Cd and Zn on the physiological changes as well as tolerance mechanisms against metal toxicity in S. caprea clones.

Effects of Ginseng Leaf Saponins on the Development of Morphine Tolerance and Dependence in Mice (인삼잎 사포닌이 몰핀의 내성 및 의존성 형성에 미치는 영향)

  • Kim, Hack-Seang;Kim, Sun-Hye;Lee, Myung-Koo;Choi, Kang-Ju;Kim, Suk-Chang
    • Journal of Ginseng Research
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    • v.13 no.1
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    • pp.8-13
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    • 1989
  • The effects of orally administered ginseng leaf saponins(GLS) on the analgesic action of morphine, the development of morphine induced tolerance and physical dependence, and the hepatic flutathione contents in mice were investigated. GLS antagonized the analgesic action of morphine and inhibited the development of morphine induced tolerance and physical dependence. It also inhibited the decrease in hepatic glutathione level induced by multiple injections of morphine.

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Antimicrobial and Antioxidant Activities in Different Parts and Cultivars of Broccoli (브로콜리 품종 및 부위에 따른 항균활성과 항산화 효과)

  • Park, Mi Young;Yoon, Moo Kyung;Kwak, Jung-Ho
    • Horticultural Science & Technology
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    • v.32 no.3
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    • pp.408-414
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    • 2014
  • The antimicrobial and antioxidant activities of different cultivars and parts of broccoli were investigated. The screening of antimicrobial activities for the floret and leaf extracts of 11 cultivars against selected gram positive (Staphylococcus aureus and Listeria monocytogenes) and gram negative bacteria (Escherichia coli O157:H7 and Salmonella enteritidis) were conducted. The AMG cultivar showed the highest antimicrobial activity against S. aureus with the floret and leaf extracts (12.83 and 13.00 mm). Antibacterial activity against L. monocytogenes was strongest with floret extract of NJ cultivar (13.58 mm) and leaf extract of YDR cultivar (13.92 mm). Moreover, the size of inhibition zone against L. monocytogenes was bigger than those of 4 kinds of pathogenic bacteria. Both floret and leaf extracts of Grd cultivar showed the highest antimicrobial activity against E. coli O157: H7, but there was no difference between floret and leaf extracts. Floret extract o f NJ cultivar and leaf extract of NY and 0c cultivars were effective against S. enteritidis whereas leaf extract exhibited better antimicrobial effect than floret extract. These results showed that floret extract of NJ and leaf extract of 0c had the highest antioxidant activity which was 39.90 and 43.64%, respectively. The antioxidant activity of leaf extract was 1.5 times higher than that of floret extract. All cultivars except NJ showed that electron donating ability of leaf extract was higher than that of floret extract.

Determination of Amino Acid Composition in Leaf, Stem, and Inflorescence of Alfalfa (Medicago sativa L.)

  • Muthusamy, Karnan;Ilavenil, Soundharrajan;Jung, Jeong Sung;Lee, Bae Hun;Nam, Cheol Hwan;Park, Hyung Soo;Choi, Ki Choon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.42 no.3
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    • pp.183-187
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    • 2022
  • Alfalfa is one of the most useful forage crops worldwide, containing a high level of amino acids that are essential to both human and animal health. However, amino acids and their concentrations may differ between plant parts. Hence, detecting amino acids in different plant parts would be useful in the development of diet supplements. The purpose of this study was to determine the amino acid content in alfalfa leaves, stems, and inflorescences using an amino acid analyzer. Asparagine and glutamic acid were the most abundant amino acids found in stems, leaves, and inflorescences than other amino acids. All parts of alfalfa had low concentrations of cysteine and methionine. All amino acids except asparagine were present in the highest concentration in leaves followed by inflorescences. Leaf had a rich amino acid content, namely asparagine, glutamic acid, leucine, proline, and lysine. However, the stem had a lower amino acid composition than the leaf or inflorescence. Overall, the data showed determining the amino acid content of forages provides a good approach to making animal feed with essential and specific amino acids and preventing excessive inclusion of amino acids.

Development of Indicator for Coastal and Estuarine Eutrophication Using Morphological Characteristics and Tissue N Content of Eelgrass, Zostera marina

  • Lee, Kun-Seop
    • ALGAE
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    • v.19 no.2
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    • pp.129-137
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    • 2004
  • Since cultural eutrophication has the detrimental effects on estuarine and coastal ecosystems, recognition of early stage of nutrient over-enrichment is critical for effective managements of the ecosystems. Since released nutrients into coastal ecosystems are diluted and dissipated through tidal action and rapid uptakes by marine plants, monitoring of in situ nutrient concentrations may not be useful for detecting early eutrophication on coastal and estuarine ecosystems. To develop an effective indicator of cultural eutrophication using marine plants, tissue N content and area normalized leaf mass of eelgrass, Zostera marina were examined in Kosung Bay and Koje Bay on the south coast of Korea from June 2001 to April 2003. Eelgrass tissue N content exhibited obvious seasonal variations. Leaf N content was highest during winter and early spring and lowest during summer. Eelgrass tissue N content was higher at Kosung Bay site, which has higher sediment organic content, than at Koje Bay site. Area normalized leaf mass showed reverse trend of leaf N content, and consequently, eelgrass leaf N content and leaf mass exhibited strong negative correlation at both study sites. The results of the present study suggested that the ratio of eelgrass leaf N content to area normalized leaf mass can be applied to assess environmental nitrogen conditions on the coastal and estuarine ecosystems.

Histological and Ultrastructural Study of Susceptible and Age-related Resistance Responses of Pepper Leaves to Colletotrichum cocodes Infection

  • Hong, Jeum-Kyu;Lee, Yeon-Kyeong;Jeun, Yong-Chull;Hwang, Byung-Kook
    • The Plant Pathology Journal
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    • v.17 no.3
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    • pp.128-140
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    • 2001
  • Infection of pepper leaves by Colletotrichum cocodes at the two- and eight-leaf stages caused susceptible and resistant lesions 96 h after inoculation, respectively. At the two-leaf stage, progressive symptom development occurred on the infected leaves. In contrast, localized necrotic spots were characteristic symptoms at the eight-leaf stage. Infected leaves at the two-leaf stage exhibited cell death accompanied by the accumulation of autofluorescent compounds. At the eight-leaf stage, pepper leaves infected by the anthracnose fungus displayed localized autofluorescence from the symptoms. Infection of pepper leaves by C. cocodes at the two-leaf stage resulted in its rapidand massive colonization of all the leaf tissues including the vascular tissue, together with cytoplasmic collapse, distortion of chloroplasts, and disruption of host cell walls. However, penetration of C. cocodes was very limited in the older leaf tissues of pepper plants at the eight-leaf stage. Fungal hyphae grew only in the intramural spaces of the epidermal cell walls at this stage. Occlusion of amorphous material in xylem vessels, aggregation of fibrillar material in inter-cellular spaces, and deposition of protein bodies were found as resistance responses to C. cocodes.

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Control of Late Leaf Spot of Groundnut (Arachis hypogaea) by Extracts from Non-Host Plant Species

  • Kishore, G.Krishna;Pande, S.;Rao, J.Narayana
    • The Plant Pathology Journal
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    • v.17 no.5
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    • pp.264-270
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    • 2001
  • The effects of leaf extracts of 14 different non-host plant species on in vitro conidial germination of Phaeoisariopsis personata, the causal organism of late leaf spot(LLS) of groundnut were evaluated. Aqueous and ethanol leaf extracts of Datura metel, Lawsonia inermis and aqueous leaf extracts of Sphaeranthus indicus at 25%(w/v) concentration completely inhibited the conidial germination of P. personata both at 24h and 48h after incubation. Aqueous leaf extracts of Blumea bifoliata, Eucalyptus globules, Ocimum sanctum and Pongamia pinnata, and ethanol leaf extracts of Azadirachta indica and S. indicus inhibited the conidial germination by >90%. Aqueous and ethanol leaf extract of L. inermis and S. indicus were highly inhibitory to conidial germination up to 1% concentration. Aqueous and ethanol leaf extracts of D. metel and ethanol leaf extract of A. indica were highly inhibitory to P. personata even at 0.01% (100 ppm) concentration. Ethanol leaf extract of A. indica up to $80^{\circ}$, aqueous leaf extracts of D. metel and S. indicus up to $100^{\circ}$, and L. inermis up to $60^{\circ}$, were highly stable and retained their fungitoxic effects. Extract of D. metel was antifungal even after 180 days when it was stored both at room temperature and $4^{\circ}$. Aqueous leaf extract of D. metel at 2% concentration effectively reduced the development of LLS by >60%, under greenhouse conditions both in prophylactic and simultaneous applications. Extracts of D. metel could be a potential economical and an eco-frendly alternative for control of late leaf spot, and its efficacy under field conditions is further being evaluated.

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