• 식물병연구
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• 제8권1호
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• pp.50-54
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• 2002

딸기탄저병의 방제방법을 개발하기 위해 배지에서 약제별 균사생장억제정도를 검정한 결과 Tricylazole수화제가 가장 좋은 효과를 보여 주었으나 딸기잎에 접종하여 효과를 검정하였을 때는 Azoxystrobin 수화제가 가장 좋은 효과를 보여주었다. 포장에서 방제효과는 관주 및 엽면살포는 방제효과가 없었으나 Azoxystrobin 수화제 1,000배액에 10분간 침지시 우수한 방제효과를 보여 주었고 처리시간과 처리농도간 약효의 차이는 볼 수 없었다.

• 한국균학회지
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• 제50권3호
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• pp.235-241
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• 2022

Anthracnose crown rot (ACR) has been observed in greenhouses during the nursery and harvest seasons in Gangwon Province, Korea. Infected plants showed black leaf spot, dark sunken pink conidial masses on petioles, wilting, and eventually death. Five isolates were obtained from the lesions of strawberry plants and were identified as a Colletotrichum gloeosporioides species complex based on their cultural and morphological characteristics. Multilocus sequence analysis of actin, calmodulin, chitin synthase, glyceraldehyde-3-phophate dehydrogenase genes, and internal transcribed spacer rDNA regions showed that the isolates formed a monophyletic group with the type strain of C. siamense. Pathogenicity tests were performed on the isolate, and Koch's postulates were performed to verify the relationship between Colletotrichum sp. and the strawberry plant variety Seolhyang. The isolate was pathogenic to strawberry plants, which exhibited typical ACR symptoms. Based on morphological characteristics, pathogenicity, and DNA sequence analyses, the fungus isolated in Korea was identified as C. siamense. This is first time C. siamense has been confirmed in ever-bearing strawberry varieties in Korea.

• 한국식물병리학회지
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• 제11권1호
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• pp.30-38
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• 1995

Anthracnose fungi associated with soybean (Glycine max), small red bean (Vigna angularis), and green bean (Phaseolus radiatus var. aurea) were compared with respect to cultural characteristics, morphological aspects, and pathogenicity. From the legumes, four different species of Colletotrichum were identified based on their morphological characters : C. truncatum, C. destructivum, C. trifolii and C. gloeosporioides. Colonies of Colletotrichum spp.on culture media varied in colour and growth habits depending upon the media and isolates. In general, formation of conidia, setae, and perithecia on water agar leaf medium was better than that on potato dextrose agar medium under near ultraviolet light. In cross inoculation tests, isolates of C. truncatum, c. destructivum, C. trifolii and C. gloeosporioides could infect soybean, small red bean, and green bean at different growth stages. There were some differences in pathogenicity or susceptibility depending on crops tested. C. truncatum was the most virulent and major pathogenic species among the four species.

• 한국약용작물학회지
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• 제18권4호
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• pp.244-247
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• 2010

It's crucial to control Alternaria blight and Anthracnose emerging mostly on ginseng leaves during the rainy season to increase the organic ginseng products. The purpose of this study is to investigate the efficay of lime-brodeaux spray on the ginseng leaves and evaluate the growth and yield of the ginseng, and the contents of ginsenoside and 70% ethanol extracts from 3-year-old ginseng variety, Cheonpoong. Lime-bordeaux sprayings were conducted in the ratio of 6-6 in June, 8-8 from July to September every 15 days. After June 10, the spraying have no effects on the growth leaf and stem, and there was no significant increase in chlorophyll contents. The ratio of intact leaf and root were distinctly increased because Alternaria blight and Anthracnose were decreased by spraying lime-bordeaux mixture. Root weight per plant and root yield were increased by 15%, and 62% in 3-year old ginseng, respectively, because the ratio of intact leaf and root were higher by using lime-bordeaux mixture. Furthermore, spraying of lime-bordeaux mixture is prone to increase the ratio of rusty root in ginseng. Spraying of lime-bordeaux mixture decreased both of the contents of ginsenoside and 70% ethanol extract by 13.7%, and 15.2% in 3-year-old ginseng, respectively.

• 식물병연구
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• 제15권2호
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• pp.88-93
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• 2009

고추 유묘의 신초가 말라죽고 잎에 수침상점무의 증상으로부터 병원균을 분리하여 균총의 색깔과 형태, 포자의 모양과 크기를 관찰한 결과 균총의 색깔은 처음에는 핑크색 띄나 차츰 회색으로 변하였고, 분생포자는 방추형으로 크기가 $8.1-17.0{\times}2.0-3.8{\mu}m$이며 생장최적온도 $25-27^{\circ}C$이다. 종 특이적 프라이머를 이용하여 고추 유묘에서 분리한 균을 동정한 결과 C. acutatum에 특이적인 프라이머인 CaINT에서만 496 bp의 증폭산물을 얻었다. 이들 결과를 바탕으로 유묘에서 분리한 균은 Collectotrichum acutatum으로 동정하였다. 고추 유묘에서 분리한 균주를 고추묘 생육단계별로 병원성 검정한 결과 유묘 뿐 아니라 식물체 전 생육기에 잎 탄저병 증상을 나타내었으며 또한 고추 열매에서도 강한 병원성을 나타내었다.

• 한국약용작물학회지
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• 제23권4호
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• pp.284-291
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• 2015

This study was performed to investigate the effects of enhanced light transmission on plant growth, photosynthetic ability, and disease tolerance to leaf blight, anthracnose in ginseng (Panax ginseng C. A. Meyer, Araliacease family) during the early growth stage (April to June). The photosynthetic ratio, stomatal conductance, and stem diameter of plants grown under a shade net with 15% light transmission rate showed an increasing trend compared to the control plants (5% light transmission rate) although the growth of the aerial parts were not influenced significantly. Plant height, stem length, and leaf length of treated plants were not significantly different from those of the control plants. Root parameters, such as root length, diameter, and weight of treated plants increased significantly compared to the control. Yield performance ($187.4kg{\cdot}10a^{-1}$) of treated plants was 55.5% higher than that of the control ($150.4kg{\cdot}10a^{-1}$). Additionally, disease severity scores of treated plants were lower than those of the control plants, revealing higher survival rates. To retain high yield potential and enhance the level of disease tolerance in ginseng, we suggest the increase of light transmission rate during the early growth stage.

• 한국약용작물학회지
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• 제20권5호
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• pp.339-344
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• 2012

This study was carried out to research microorganisms having the antifungal activity against ginseng Alternaria blight pathogen Alternaria panax and ginseng anthracnose pathogen Colletotrichum gloeosporioides. Eleven Bacillus strains. were isolated from Korean traditional soybean paste and Kimchi. Among the 11 isolates, DJ5, KC1, KC2 and KC4 showing antagonistic activity on the mycelial growth of A. panax and C. gloeosporioides in pairing culture were finally selected as the antagonistic microorganisms. Based on 16s rRNA sequence and phylogenetic tree analysis, they were identified as Bacillus spp.. The selected microorganisms were investigated antagonistic activity by measured leaf-segment colonization in pot test. When Bacillus sp. were injected after A. panax treatment, KC1, KC2 and KC4 showed similar effect to chemical pesticides treated control. To measure preventive effect of Bacillus sp, antagonistic microorganisms were injected and C. gloeosporioides was treated in pot. When measuring the effectiveness for the prevention of Anthracnose, All Bacillus spp. showed approximately 83~90 % degree of superior preventive effect. In general, The four Bacillus spp. isolated from Korean traditional fermented foods showed therapeutic effect of Alternaria blight and preventive effect of Anthracnose.

• 한국균학회지
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• 제47권4호
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• pp.393-406
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• 2019

2017년 8월경, 강원도 홍천군, 정선군, 평창군 소재 무 재배 포장에서 잎자루와 잎의 주맥에 형성된 탄저병 병징이 관찰되었다. 회색 내지 짙은 갈색을 띤 1-2 mm 크기의 점무늬가 아래 잎과 잎의 주맥에 형성되었다. 이러한 점무늬는 점차 확대되고 합쳐지면서 갈색을 띤 불규칙한 병반으로 나타났다. 병든 무 잎에서 10개의 Colletotrichum균을 분리하였다. 분리 균주의 형태적 특성과 internal transcribed spacers and intervening 5.8S rDNA (ITS), partial beta-tubulin gene (TUB2), partial actin gene (ACT) 및 partial chitin synthase-1 gene (CHS-1) 부위를 사용한 다자위 분자 계통 분석(multilocus molecular phylogenetic analysis)에 의해 8개 균주는 Colletotrichum higginsianum, 2개 균주는C. truncatum으로 동정되었다. 무(시래기무, 오사리무)와 배추(품종: 추노, 스마트)를 대상으로 상처와 무상처로 구분하여 포자현탁액을 떨어뜨려서 접종하는 방법을 사용하여 병원성 검정을 수행하였다. 무에서는C. truncatum 1개 균주를 제외하고 상처와 무상처 접종에서 모두 병징을 나타냈고, 배추의 경우는C. higginsianum 접종구에서만 상처의 유무에 관계없이 병이 발생하였다. 국내에는 기존에 C. higginsianum은 무와 배추의 탄저병균으로 보고되었으며, C. truncatum에 의한 무 탄저병의 발생은 본 연구에서 국내 최초로 보고한다.

• The Plant Pathology Journal
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• 제24권2호
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• pp.125-130
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• 2008

Various kinds of leaf spots and blights were found in kiwifruit (Actinidia deliciosa) orchards on 2006 in Korea. Disease incidences were quite variable between open-field and rain-proof shelter. Rate of diseased leaves was recorded as about 70% at open-field orchards in late season but use of rain-proof vinyl shelters alleviated the disease incidences by 20%. Angular leaf spots appeared at early infection stage on June and several other symptoms were also recognized as the disease developed afterward. On September, brown leaf blights were the most frequent, followed by grayish brown ring spots, silvering gray leaf blights, zonate leaf blights, dark brown ring spots and angular leaf spots at open-field orchards. Four fungal species were frequently isolated from the disease symptoms. Phomopsis sp. was the most predominant fungus associated with the leaf spot and blight symptoms on kiwifruit, followed by Glomerella cingulata, Alternaria alternata and Pestalo-tiopsis sp. Phomopsis sp. was commonly isolated from angular leaf spots, silvering gray leaf blights, and zonate brown leaf blights. G. cingulata, A. alternata and Pestalotiopsis sp. were isolated from grayish brown ring spots (anthracnose), brown ring spots and zonate dark brown leaf blights. Typical symptoms appeared on the wounded and unwounded leaves, which were inoculated by each of Phomopsis sp., G. cingulata, and Pestalotiopsis sp., but A. alternata caused symptoms only on the wounded leaves.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 추계학술발표회
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• pp.342-343
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• 2011